DHX38

DHX38
Identifiers
Aliases	DHX38 , DDX38, PRP16, PRPF16, DEAH-box helicase 38, RP84
External IDs	OMIM: 605584 MGI: 1927617 HomoloGene: 8512 GeneCards: DHX38
Gene location (Human) Chr. Chromosome 16 (human) [1] Band 16q22.2 Start 72,093,613 bp [1] End 72,112,912 bp [1]
Gene location (Mouse) Chr. Chromosome 8 (mouse) [2] Band 8|8 D3 Start 110,274,643 bp [2] End 110,292,493 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in sural nerve Achilles tendon skin of abdomen left uterine tube ganglionic eminence gastric mucosa spleen left lobe of thyroid gland right uterine tube transverse colon Top expressed in yolk sac secondary oocyte spermatid spermatocyte lip primitive streak superior frontal gyrus ascending aorta mirror duodenum More reference expression data BioGPS More reference expression data
Gene ontology Molecular function nucleotide binding helicase activity protein binding nucleic acid binding hydrolase activity ATP binding RNA binding 3'-5' RNA helicase activity Cellular component catalytic step 2 spliceosome membrane nucleoplasm spliceosomal complex nucleus cytoplasm Biological process mRNA splicing, via spliceosome termination of RNA polymerase II transcription mRNA processing mRNA export from nucleus mRNA 3'-end processing RNA splicing RNA export from nucleus Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 9785 64340 Ensembl ENSG00000140829 ENSMUSG00000037993 UniProt Q92620 Q80X98 RefSeq (mRNA) NM_014003 NM_178380 RefSeq (protein) NP_054722 NP_848467 Location (UCSC) Chr 16: 72.09 – 72.11 Mb Chr 8: 110.27 – 110.29 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 is an enzyme that in humans is encoded by the DHX38 gene. ^{[5] [6] [7]}

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a member of the DEAD/H box family of splicing factors. This protein resembles yeast Prp16 more closely than other DEAD/H family members. It is an ATPase and essential for the catalytic step II in pre-mRNA splicing process. ^[7]

References

1. GRCh38: Ensembl release 89: ENSG00000140829 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000037993 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Zhou Z, Reed R (Jun 1998). "Human homologs of yeast prp16 and prp17 reveal conservation of the mechanism for catalytic step II of pre-mRNA splicing". EMBO J. 17 (7): 2095–106. doi:10.1093/emboj/17.7.2095. PMC   1170554 . PMID   9524131.
6. Nagase T, Seki N, Ishikawa K, Ohira M, Kawarabayasi Y, Ohara O, Tanaka A, Kotani H, Miyajima N, Nomura N (May 1997). "Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain". DNA Res. 3 (5): 321–9, 341–54. doi: 10.1093/dnares/3.5.321 . PMID   9039502.
7. "Entrez Gene: DHX38 DEAH (Asp-Glu-Ala-His) box polypeptide 38".

Further reading

• Schwer B, Guthrie C (1991). "PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosome". Nature. 349 (6309): 494–9. Bibcode:1991Natur.349..494S. doi:10.1038/349494a0. PMID   1825134. S2CID   4281489.
• Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi: 10.1101/gr.6.9.791 . PMID   8889548.
• Hillier LD, Lennon G, Becker M, et al. (1997). "Generation and analysis of 280,000 human expressed sequence tags". Genome Res. 6 (9): 807–28. doi: 10.1101/gr.6.9.807 . PMID   8889549.
• Gee S, Krauss SW, Miller E, et al. (1997). "Cloning of mDEAH9, a putative RNA helicase and mammalian homologue of Saccharomyces cerevisiae splicing factor Prp43". Proc. Natl. Acad. Sci. U.S.A. 94 (22): 11803–7. Bibcode:1997PNAS...9411803G. doi: 10.1073/pnas.94.22.11803 . PMC   23596 . PMID   9342318.
• Wang Y, Guthrie C (1998). "PRP16, a DEAH-box RNA helicase, is recruited to the spliceosome primarily via its nonconserved N-terminal domain". RNA. 4 (10): 1216–29. doi:10.1017/S1355838298980992. PMC   1369694 . PMID   9769096.
• Orphanides G, Wu WH, Lane WS, et al. (1999). "The chromatin-specific transcription elongation factor FACT comprises human SPT16 and SSRP1 proteins". Nature. 400 (6741): 284–8. Bibcode:1999Natur.400..284O. doi:10.1038/22350. PMID   10421373. S2CID   4300397.
• Loftus BJ, Kim UJ, Sneddon VP, et al. (1999). "Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q". Genomics. 60 (3): 295–308. doi:10.1006/geno.1999.5927. PMID   10493829.
• Jurica MS, Licklider LJ, Gygi SR, et al. (2002). "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis". RNA. 8 (4): 426–39. doi:10.1017/S1355838202021088. PMC   1370266 . PMID   11991638.
• Chagnon P, Michaud J, Mitchell G, et al. (2003). "A missense mutation (R565W) in cirhin (FLJ14728) in North American Indian childhood cirrhosis". Am. J. Hum. Genet. 71 (6): 1443–9. doi:10.1086/344580. PMC   378590 . PMID   12417987.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Obuse C, Yang H, Nozaki N, et al. (2004). "Proteomics analysis of the centromere complex from HeLa interphase cells: UV-damaged DNA binding protein 1 (DDB-1) is a component of the CEN-complex, while BMI-1 is transiently co-localized with the centromeric region in interphase". Genes Cells. 9 (2): 105–20. doi: 10.1111/j.1365-2443.2004.00705.x . PMID   15009096. S2CID   21813024.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC   528928 . PMID   15489334.
• Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC   1847948 . PMID   17353931.