ECT2

ECT2
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3L46, 4N40
Identifiers
Aliases	ECT2 , ARHGEF31, epithelial cell transforming 2
External IDs	OMIM: 600586; MGI: 95281; HomoloGene: 7298; GeneCards: ECT2; OMA:ECT2 - orthologs
Gene location (Mouse) Chr. Chromosome 3 (mouse) [1] Band 3|3 A3 Start 27,151,371 bp [1] End 27,208,027 bp [1]
RNA expression pattern Bgee Human Mouse (ortholog) n/a Top expressed in medial ganglionic eminence zygote secondary oocyte primary oocyte ventricular zone tail of embryo cumulus cell genital tubercle abdominal wall Gonadal ridge BioGPS More reference expression data
Gene ontology Molecular function protein homodimerization activity GTPase activator activity signal transducer activity protein binding guanyl-nucleotide exchange factor activity Cellular component cytoplasm centralspindlin complex cell-cell junction bicellular tight junction spindle cell junction midbody cleavage furrow mitotic spindle cytoskeleton nucleus cytosol Biological process cellular response to calcium ion cell differentiation intracellular signal transduction activation of GTPase activity nervous system development cell division positive regulation of cytokinesis positive regulation of GTPase activity positive regulation of neuron differentiation bicellular tight junction assembly cell morphogenesis positive regulation of apoptotic process cellular response to ionizing radiation protein transport positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of protein import into nucleus cell cycle regulation of protein kinase activity regulation of Rho protein signal transduction regulation of attachment of spindle microtubules to kinetochore regulation of small GTPase mediated signal transduction protein homooligomerization activation of protein kinase activity cellular response to hydrogen peroxide transport mitotic cytokinesis cytokinesis G protein-coupled receptor signaling pathway Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 1894 13605 Ensembl ENSG00000114346 ENSMUSG00000027699 UniProt Q9H8V3 Q07139 RefSeq (mRNA) NM_001258315 NM_001258316 NM_018098 NM_001177625 NM_001177626 NM_007900 RefSeq (protein) NP_001245244 NP_001245245 NP_060568 NP_001336023 NP_001336024 NP_001336025 NP_001336026 NP_001336027 NP_001336028 NP_001336029 NP_001336030 NP_001336031 NP_001336032 NP_001336033 NP_001171096 NP_001171097 NP_031926 Location (UCSC) n/a Chr 3: 27.15 – 27.21 Mb PubMed search [2] [3]
Wikidata
View/Edit Human View/Edit Mouse

Protein ECT2 is a protein that in humans is encoded by the ECT2 gene. ^{[4] [5] [6]}

Function

The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. ^[6]

Interactions

ECT2 has been shown to interact with PARD6A. ^[7]

References

1. GRCm38: Ensembl release 89: ENSMUSG00000027699 – Ensembl, May 2017
2. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
3. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. Miki T, Smith CL, Long JE, Eva A, Fleming TP (Apr 1993). "Oncogene ect2 is related to regulators of small GTP-binding proteins". Nature. 362 (6419): 462–5. Bibcode:1993Natur.362..462M. doi:10.1038/362462a0. PMID   8464478. S2CID   722736.
5. Tatsumoto T, Xie X, Blumenthal R, Okamoto I, Miki T (Dec 1999). "Human ECT2 is an exchange factor for Rho GTPases, phosphorylated in G2/M phases, and involved in cytokinesis". J. Cell Biol. 147 (5): 921–8. doi:10.1083/jcb.147.5.921. PMC   2169345 . PMID   10579713.
6. "Entrez Gene: ECT2 epithelial cell transforming sequence 2 oncogene".
7. Liu XF, Ishida H, Raziuddin R, Miki T (Aug 2004). "Nucleotide exchange factor ECT2 interacts with the polarity protein complex Par6/Par3/protein kinase Czeta (PKCzeta) and regulates PKCzeta activity". Mol. Cell. Biol. 24 (15): 6665–75. doi:10.1128/MCB.24.15.6665-6675.2004. PMC   444862 . PMID   15254234.

Further reading

• Takai S, Long JE, Yamada K, Miki T (1995). "Chromosomal localization of the human ECT2 proto-oncogene to 3q26.1-->q26.2 by somatic cell analysis and fluorescence in situ hybridization". Genomics. 27 (1): 220–2. doi:10.1006/geno.1995.1033. PMID   7665179.
• Hillier LD, Lennon G, Becker M, Bonaldo MF, Chiapelli B, Chissoe S, Dietrich N, DuBuque T, Favello A, Gish W, Hawkins M, Hultman M, Kucaba T, Lacy M, Le M, Le N, Mardis E, Moore B, Morris M, Parsons J, Prange C, Rifkin L, Rohlfing T, Schellenberg K, Bento Soares M, Tan F, Thierry-Meg J, Trevaskis E, Underwood K, Wohldman P, Waterston R, Wilson R, Marra M (1996). "Generation and analysis of 280,000 human expressed sequence tags". Genome Res. 6 (9): 807–28. doi: 10.1101/gr.6.9.807 . PMID   8889549.
• Kimura K, Tsuji T, Takada Y, Miki T, Narumiya S (2000). "Accumulation of GTP-bound RhoA during cytokinesis and a critical role of ECT2 in this accumulation". J. Biol. Chem. 275 (23): 17233–6. doi: 10.1074/jbc.C000212200 . PMID   10837491.
• Wennerberg K, Ellerbroek SM, Liu RY, Karnoub AE, Burridge K, Der CJ (2002). "RhoG signals in parallel with Rac1 and Cdc42". J. Biol. Chem. 277 (49): 47810–7. doi: 10.1074/jbc.M203816200 . PMID   12376551.
• Matsuda A, Suzuki Y, Honda G, Muramatsu S, Matsuzaki O, Nagano Y, Doi T, Shimotohno K, Harada T, Nishida E, Hayashi H, Sugano S (2003). "Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways". Oncogene. 22 (21): 3307–18. doi:10.1038/sj.onc.1206406. PMID   12761501. S2CID   38880905.
• Saito S, Tatsumoto T, Lorenzi MV, Chedid M, Kapoor V, Sakata H, Rubin J, Miki T (2003). "Rho exchange factor ECT2 is induced by growth factors and regulates cytokinesis through the N-terminal cell cycle regulator-related domains". J. Cell. Biochem. 90 (4): 819–36. doi:10.1002/jcb.10688. PMID   14587037. S2CID   1295995.
• Hara T, Ishida H, Raziuddin R, Dorkhom S, Kamijo K, Miki T (2004). "Novel kelch-like protein, KLEIP, is involved in actin assembly at cell-cell contact sites of Madin-Darby canine kidney cells". Mol. Biol. Cell. 15 (3): 1172–84. doi:10.1091/mbc.E03-07-0531. PMC   363103 . PMID   14668487.
• Liu XF, Ishida H, Raziuddin R, Miki T (2004). "Nucleotide exchange factor ECT2 interacts with the polarity protein complex Par6/Par3/protein kinase Czeta (PKCzeta) and regulates PKCzeta activity". Mol. Cell. Biol. 24 (15): 6665–75. doi:10.1128/MCB.24.15.6665-6675.2004. PMC   444862 . PMID   15254234.
• Kim JE, Billadeau DD, Chen J (2005). "The tandem BRCT domains of Ect2 are required for both negative and positive regulation of Ect2 in cytokinesis". J. Biol. Chem. 280 (7): 5733–9. doi: 10.1074/jbc.M409298200 . PMID   15545273.
• Oceguera-Yanez F, Kimura K, Yasuda S, Higashida C, Kitamura T, Hiraoka Y, Haraguchi T, Narumiya S (2005). "Ect2 and MgcRacGAP regulate the activation and function of Cdc42 in mitosis". J. Cell Biol. 168 (2): 221–32. doi:10.1083/jcb.200408085. PMC   2171585 . PMID   15642749.
• Yüce O, Piekny A, Glotzer M (2005). "An ECT2-centralspindlin complex regulates the localization and function of RhoA". J. Cell Biol. 170 (4): 571–82. doi:10.1083/jcb.200501097. PMC   2171506 . PMID   16103226.
• Niiya F, Xie X, Lee KS, Inoue H, Miki T (2005). "Inhibition of cyclin-dependent kinase 1 induces cytokinesis without chromosome segregation in an ECT2 and MgcRacGAP-dependent manner". J. Biol. Chem. 280 (43): 36502–9. doi: 10.1074/jbc.M508007200 . PMID   16118207.
• Zhao WM, Fang G (2005). "MgcRacGAP controls the assembly of the contractile ring and the initiation of cytokinesis". Proc. Natl. Acad. Sci. U.S.A. 102 (37): 13158–63. Bibcode:2005PNAS..10213158Z. doi: 10.1073/pnas.0504145102 . PMC   1201590 . PMID   16129829.
• Hara T, Abe M, Inoue H, Yu LR, Veenstra TD, Kang YH, Lee KS, Miki T (2006). "Cytokinesis regulator ECT2 changes its conformation through phosphorylation at Thr-341 in G2/M phase". Oncogene. 25 (4): 566–78. doi: 10.1038/sj.onc.1209078 . PMID   16170345.
• Niiya F, Tatsumoto T, Lee KS, Miki T (2006). "Phosphorylation of the cytokinesis regulator ECT2 at G2/M phase stimulates association of the mitotic kinase Plk1 and accumulation of GTP-bound RhoA". Oncogene. 25 (6): 827–37. doi:10.1038/sj.onc.1209124. PMID   16247472. S2CID   28079334.
• Nishimura Y, Yonemura S (2006). "Centralspindlin regulates ECT2 and RhoA accumulation at the equatorial cortex during cytokinesis". J. Cell Sci. 119 (Pt 1): 104–14. doi:10.1242/jcs.02737. PMID   16352658. S2CID   14865639.