ELOC

ELOC
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1LM8, 1LQB, 1VCB, 2C9W, 2IZV, 2MA9, 3DCG, 3ZKJ, 3ZNG, 3ZRC, 3ZRF, 3ZTC, 3ZTD, 3ZUN, 4AJY, 4AWJ, 4B95, 4B9K, 4BKS, 4BKT, 4N9F, 4W9C, 4W9D, 4W9E, 4W9F, 4W9G, 4W9H, 4W9I, 4W9J, 4W9K, 4W9L, 4WQO, 5BO4
Identifiers
Aliases	ELOC , SIII, eloC, TCEB1, transcription elongation factor B subunit 1, elongin C
External IDs	OMIM: 600788; MGI: 1915173; HomoloGene: 38083; GeneCards: ELOC; OMA:ELOC - orthologs
Gene location (Human) Chr. Chromosome 8 (human) [1] Band 8q21.11 Start 73,939,169 bp [1] End 73,972,307 bp [1]
Gene location (Mouse) Chr. Chromosome 1 (mouse) [2] Band 1|1 A3 Start 16,711,949 bp [2] End 16,727,266 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in oocyte sperm right ventricle gingival epithelium palpebral conjunctiva left testis right testis tendon of biceps brachii biceps brachii amniotic fluid Top expressed in morula primary oocyte blastocyst primitive streak endothelial cell of lymphatic vessel epithelium of stomach yolk sac facial motor nucleus medial ganglionic eminence atrioventricular valve More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein binding ubiquitin-protein transferase activity Cellular component cytosol nucleus elongin complex nucleoplasm Biological process regulation of transcription by RNA polymerase II transcription elongation from RNA polymerase II promoter regulation of transcription, DNA-templated transcription by RNA polymerase II ubiquitin-dependent protein catabolic process transcription, DNA-templated regulation of transcription from RNA polymerase II promoter in response to hypoxia viral process positive regulation of transcription elongation from RNA polymerase II promoter post-translational protein modification protein ubiquitination Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 6921 67923 Ensembl ENSG00000154582 ENSMUSG00000079658 UniProt Q15369 P83940 RefSeq (mRNA) NM_001204857 NM_001204858 NM_001204859 NM_001204860 NM_001204861 NM_001204862 NM_001204863 NM_001204864 NM_005648 NM_001310470 NM_026456 RefSeq (protein) NP_001191786 NP_001191787 NP_001191788 NP_001191789 NP_001191790 NP_001191791 NP_001191792 NP_001191793 NP_005639 NP_001297399 NP_080732 Location (UCSC) Chr 8: 73.94 – 73.97 Mb Chr 1: 16.71 – 16.73 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Elongin C is a protein that in humans is encoded by the ELOC gene. ^{[5] [6]}

Function

Elongin C is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. ^[7]

Interactions

TCEB1 has been shown to interact with:

• TCEB2 ^{[8] [9] [10] [11]} and
• Von Hippel-Lindau tumor suppressor. ^{[9] [11] [12] [13] [14] [15] [16]}

References

1. GRCh38: Ensembl release 89: ENSG00000154582 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000079658 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Garrett KP, Haque D, Conaway RC, Conaway JW (Feb 1995). "A human cDNA encoding the small subunit of RNA polymerase II transcription factor SIII". Gene. 150 (2): 413–4. doi:10.1016/0378-1119(94)90467-7. PMID   7821821.
6. Duan DR, Pause A, Burgess WH, Aso T, Chen DY, Garrett KP, Conaway RC, Conaway JW, Linehan WM, Klausner RD (Oct 1995). "Inhibition of transcription elongation by the VHL tumor suppressor protein". Science. 269 (5229): 1402–6. Bibcode:1995Sci...269.1402D. doi:10.1126/science.7660122. PMID   7660122. S2CID   22334719.
7. "Entrez Gene: TCEB1 transcription elongation factor B (SIII), polypeptide 1 (15kDa, elongin C)".
8. Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID   16189514. S2CID   4427026.
9. Ohh M, Takagi Y, Aso T, Stebbins CE, Pavletich NP, Zbar B, Conaway RC, Conaway JW, Kaelin WG (Dec 1999). "Synthetic peptides define critical contacts between elongin C, elongin B, and the von Hippel-Lindau protein". J. Clin. Invest. 104 (11): 1583–91. doi:10.1172/JCI8161. PMC   481054 . PMID   10587522.
10. Krumm A, Groudine M (Sep 1995). "Tumor suppression and transcription elongation: the dire consequences of changing partners". Science. 269 (5229): 1400–1. Bibcode:1995Sci...269.1400K. doi:10.1126/science.7660121. PMID   7660121. S2CID   39758696.
11. Li Z, Na X, Wang D, Schoen SR, Messing EM, Wu G (Feb 2002). "Ubiquitination of a novel deubiquitinating enzyme requires direct binding to von Hippel-Lindau tumor suppressor protein". J. Biol. Chem. 277 (7): 4656–62. doi: 10.1074/jbc.M108269200 . PMID   11739384.
12. Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3: 89. doi:10.1038/msb4100134. PMC   1847948 . PMID   17353931.
13. Tsuchiya H, Iseda T, Hino O (Jul 1996). "Identification of a novel protein (VBP-1) binding to the von Hippel-Lindau (VHL) tumor suppressor gene product". Cancer Res. 56 (13): 2881–5. PMID   8674032.
14. Min JH, Yang H, Ivan M, Gertler F, Kaelin WG, Pavletich NP (Jun 2002). "Structure of an HIF-1alpha -pVHL complex: hydroxyproline recognition in signaling". Science. 296 (5574): 1886–9. Bibcode:2002Sci...296.1886M. doi: 10.1126/science.1073440 . PMID   12004076. S2CID   19641938.
15. Hacker KE, Lee CM, Rathmell WK (2008). Zhang B (ed.). "VHL type 2B mutations retain VBC complex form and function". PLOS ONE. 3 (11) e3801. Bibcode:2008PLoSO...3.3801H. doi: 10.1371/journal.pone.0003801 . PMC   2583047 . PMID   19030229.
16. Kim BY, Kim H, Cho EJ, Youn HD (Feb 2008). "Nur77 upregulates HIF-alpha by inhibiting pVHL-mediated degradation". Exp. Mol. Med. 40 (1): 71–83. doi:10.3858/emm.2008.40.1.71. PMC   2679322 . PMID   18305400.

Further reading

• Aso T, Lane WS, Conaway JW, Conaway RC (1995). "Elongin (SIII): a multisubunit regulator of elongation by RNA polymerase II". Science. 269 (5229): 1439–43. Bibcode:1995Sci...269.1439A. doi:10.1126/science.7660129. PMID   7660129. S2CID   37649908.
• Kibel A, Iliopoulos O, DeCaprio JA, Kaelin WG (1995). "Binding of the von Hippel-Lindau tumor suppressor protein to Elongin B and C". Science. 269 (5229): 1444–6. Bibcode:1995Sci...269.1444K. doi:10.1126/science.7660130. PMID   7660130. S2CID   25410108.
• Bradsher JN, Jackson KW, Conaway RC, Conaway JW (1994). "RNA polymerase II transcription factor SIII. I. Identification, purification, and properties". J. Biol. Chem. 268 (34): 25587–93. doi: 10.1016/S0021-9258(19)74431-7 . PMID   8244996.
• Pause A, Lee S, Worrell RA, Chen DY, Burgess WH, Linehan WM, Klausner RD (1997). "The von Hippel-Lindau tumor-suppressor gene product forms a stable complex with human CUL-2, a member of the Cdc53 family of proteins". Proc. Natl. Acad. Sci. U.S.A. 94 (6): 2156–61. Bibcode:1997PNAS...94.2156P. doi: 10.1073/pnas.94.6.2156 . PMC   20057 . PMID   9122164.
• Lonergan KM, Iliopoulos O, Ohh M, Kamura T, Conaway RC, Conaway JW, Kaelin WG (1998). "Regulation of hypoxia-inducible mRNAs by the von Hippel-Lindau tumor suppressor protein requires binding to complexes containing elongins B/C and Cul2". Mol. Cell. Biol. 18 (2): 732–41. doi:10.1128/mcb.18.2.732. PMC   108784 . PMID   9447969.
• Zhang JG, Farley A, Nicholson SE, Willson TA, Zugaro LM, Simpson RJ, Moritz RL, Cary D, Richardson R, Hausmann G, Kile BJ, Kent SB, Alexander WS, Metcalf D, Hilton DJ, Nicola NA, Baca M (1999). "The conserved SOCS box motif in suppressors of cytokine signaling binds to elongins B and C and may couple bound proteins to proteasomal degradation". Proc. Natl. Acad. Sci. U.S.A. 96 (5): 2071–6. Bibcode:1999PNAS...96.2071Z. doi: 10.1073/pnas.96.5.2071 . PMC   26738 . PMID   10051596.
• Stebbins CE, Kaelin WG, Pavletich NP (1999). "Structure of the VHL-ElonginC-ElonginB complex: implications for VHL tumor suppressor function". Science. 284 (5413): 455–61. Bibcode:1999Sci...284..455S. doi:10.1126/science.284.5413.455. PMID   10205047.
• Pause A, Peterson B, Schaffar G, Stearman R, Klausner RD (1999). "Studying interactions of four proteins in the yeast two-hybrid system: structural resemblance of the pVHL/elongin BC/hCUL-2 complex with the ubiquitin ligase complex SKP1/cullin/F-box protein". Proc. Natl. Acad. Sci. U.S.A. 96 (17): 9533–8. Bibcode:1999PNAS...96.9533P. doi: 10.1073/pnas.96.17.9533 . PMC   22243 . PMID   10449727.
• Iwai K, Yamanaka K, Kamura T, Minato N, Conaway RC, Conaway JW, Klausner RD, Pause A (1999). "Identification of the von Hippel-lindau tumor-suppressor protein as part of an active E3 ubiquitin ligase complex". Proc. Natl. Acad. Sci. U.S.A. 96 (22): 12436–41. Bibcode:1999PNAS...9612436I. doi: 10.1073/pnas.96.22.12436 . PMC   22941 . PMID   10535940.
• Ohh M, Takagi Y, Aso T, Stebbins CE, Pavletich NP, Zbar B, Conaway RC, Conaway JW, Kaelin WG (2000). "Synthetic peptides define critical contacts between elongin C, elongin B, and the von Hippel-Lindau protein". J. Clin. Invest. 104 (11): 1583–91. doi:10.1172/JCI8161. PMC   481054 . PMID   10587522.
• Aso T, Yamazaki K, Amimoto K, Kuroiwa A, Higashi H, Matsuda Y, Kitajima S, Hatakeyama M (2000). "Identification and characterization of Elongin A2, a new member of the Elongin family of transcription elongation factors, specifically expressed in the testis". J. Biol. Chem. 275 (9): 6546–52. doi: 10.1074/jbc.275.9.6546 . PMID   10692460.
• Adryan B, Decker HJ, Papas TS, Hsu T (2000). "Tracheal development and the von Hippel-Lindau tumor suppressor homolog in Drosophila". Oncogene. 19 (24): 2803–11. doi: 10.1038/sj.onc.1203611 . PMID   10851083.
• Kamura T, Sato S, Iwai K, Czyzyk-Krzeska M, Conaway RC, Conaway JW (2000). "Activation of HIF1alpha ubiquitination by a reconstituted von Hippel-Lindau (VHL) tumor suppressor complex". Proc. Natl. Acad. Sci. U.S.A. 97 (19): 10430–5. Bibcode:2000PNAS...9710430K. doi: 10.1073/pnas.190332597 . PMC   27041 . PMID   10973499.
• Aso T, Yamazaki K, Aigaki T, Kitajima S (2000). "Drosophila von Hippel-Lindau tumor suppressor complex possesses E3 ubiquitin ligase activity". Biochem. Biophys. Res. Commun. 276 (1): 355–61. doi:10.1006/bbrc.2000.3451. PMID   11006129.
• Kamura T, Burian D, Yan Q, Schmidt SL, Lane WS, Querido E, Branton PE, Shilatifard A, Conaway RC, Conaway JW (2001). "Muf1, a novel Elongin BC-interacting leucine-rich repeat protein that can assemble with Cul5 and Rbx1 to reconstitute a ubiquitin ligase". J. Biol. Chem. 276 (32): 29748–53. doi: 10.1074/jbc.M103093200 . PMID   11384984.
• Li Z, Na X, Wang D, Schoen SR, Messing EM, Wu G (2002). "Ubiquitination of a novel deubiquitinating enzyme requires direct binding to von Hippel-Lindau tumor suppressor protein". J. Biol. Chem. 277 (7): 4656–62. doi: 10.1074/jbc.M108269200 . PMID   11739384.
• Yamazaki K, Guo L, Sugahara K, Zhang C, Enzan H, Nakabeppu Y, Kitajima S, Aso T (2002). "Identification and biochemical characterization of a novel transcription elongation factor, Elongin A3". J. Biol. Chem. 277 (29): 26444–51. doi: 10.1074/jbc.M202859200 . PMID   11994304.
• Porkka K, Saramäki O, Tanner M, Visakorpi T (2002). "Amplification and overexpression of Elongin C gene discovered in prostate cancer by cDNA microarrays". Lab. Invest. 82 (5): 629–37. doi: 10.1038/labinvest.3780457 . PMID   12004003.