GIT2

GIT2
Identifiers
Aliases	GIT2 , CAT-2, CAT2, PKL, GIT ArfGAP 2
External IDs	OMIM: 608564 MGI: 1347053 HomoloGene: 41336 GeneCards: GIT2
Gene location (Human)
Chr.	Chromosome 12 (human)
End	109,996,389 bp
Gene location (Mouse)
Chr.	Chromosome 5 (mouse)
End	114,775,517 bp
RNA expression pattern
	Top expressed in
	monocyte; ; middle temporal gyrus; ; Brodmann area 23; ; blood; ; body of pancreas; ; visceral pleura; ; spleen; ; bone marrow; ; secondary oocyte; ; bone marrow cells;
	Top expressed in
	thymus; ; internal carotid artery; ; secondary oocyte; ; external carotid artery; ; cumulus cell; ; right lung; ; spleen; ; lymph node; ; blood; ; right lung lobe;
	More reference expression data
	; ;
	More reference expression data
Gene ontology
Molecular function	protein binding ; metal ion binding ; GTPase activator activity ;
Cellular component	focal adhesion ; nucleoplasm ;
Biological process	positive regulation of GTPase activity ; regulation of G protein-coupled receptor signaling pathway ;
	Sources:Amigo / QuickGO
Orthologs
	9815
	26431
	ENSG00000139436
	ENSMUSG00000041890
	Q14161
	Q9JLQ2
NM_001135213 ; NM_001135214 ; NM_014776 ; NM_057169 ; NM_057170 ;
	NM_139201 ; NM_001330153 ; NM_001330154
	NM_001077359 ; NM_001077360 ; NM_019834 ; NM_001347400
NP_001128685 ; NP_001128686 ; NP_001317082 ; NP_001317083 ; NP_055591 ;
	NP_476510 ; NP_476511 ; NP_631940
	NP_001070827 ; NP_001070828 ; NP_001334329 ; NP_062808
	Wikidata
View/Edit Human	View/Edit Mouse

Last updated January 12, 2024

ARF GTPase-activating protein GIT2 is an enzyme that in humans is encoded by the GIT2 gene.^[5]^[6]^[7]

Function

This gene encodes a member of the GIT protein family. GIT proteins interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. This gene undergoes extensive alternative splicing; although ten transcript variants have been described, the full length sequence has been determined for only four variants. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding.^[7]

Interactions

GIT2 has been shown to interact with GIT1.^[8]

Related Research Articles

ADP-ribosylation factor 6 (ARF6) is a member of the ADP ribosylation factor family of GTP-binding proteins. ARF6 has a variety of cellular functions that are frequently involved in trafficking of biological membranes and transmembrane protein cargo. ARF6 has specifically been implicated in endocytosis of plasma membrane proteins and also, to a lesser extent, plasma membrane protein recycling.

G-protein-coupled receptor kinase 2 (GRK2) is an enzyme that in humans is encoded by the ADRBK1 gene. GRK2 was initially called Beta-adrenergic receptor kinase, and is a member of the G protein-coupled receptor kinase subfamily of the Ser/Thr protein kinases that is most highly similar to GRK3(βARK2).

ADP-ribosylation factor 1 is a protein that in humans is encoded by the ARF1 gene.

Beta-arrestin-2, also known as arrestin beta-2, is an intracellular protein that in humans is encoded by the ARRB2 gene.

Arrestin, beta 1, also known as ARRB1, is a protein which in humans is encoded by the ARRB1 gene.

Rho guanine nucleotide exchange factor 7 is a protein that in humans is encoded by the ARHGEF7 gene.

Rho guanine nucleotide exchange factor 6 is a protein that, in humans, is encoded by the ARHGEF6 gene.

This gene encodes a member of the G protein-coupled receptor kinase subfamily of the Ser/Thr protein kinase family, and is most highly similar to GRK4 and GRK5. The protein phosphorylates the activated forms of G protein-coupled receptors to regulate their signaling.

ARF GTPase-activating protein GIT1 is an enzyme that in humans is encoded by the GIT1 gene.

Cytohesin-2 is a protein that in humans is encoded by the CYTH2 gene.

G protein-coupled receptor kinase 5 is a member of the G protein-coupled receptor kinase subfamily of the Ser/Thr protein kinases, and is most highly similar to GRK4 and GRK6. The protein phosphorylates the activated forms of G protein-coupled receptors to regulate their signaling.

Cytohesin-1 formerly known as Pleckstrin homology, Sec7 and coiled/coil domains 1 (PSCD1) is a protein that in humans is encoded by the CYTH1 gene.

ADP-ribosylation factor GTPase-activating protein 1 is an enzyme that in humans is encoded by the ARFGAP1 gene. Two transcript variants encoding different isoforms have been found for this gene.

Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 is a protein that in humans is encoded by the AGAP2 gene.

ADP-ribosylation factor 5 is a protein that in humans is encoded by the ARF5 gene.

ADP-ribosylation factor 4 is a protein that in humans is encoded by the ARF4 gene.

Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 is a protein that in humans is encoded by the ASAP2 gene.

ADP-ribosylation factor-related protein 1 is a protein that in humans is encoded by the ARFRP1 gene.

Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 is an enzyme that in humans is encoded by the AGAP1 gene.

G-protein-coupled receptor kinase 3 (GRK3) is an enzyme that in humans is encoded by the ADRBK2 gene. GRK3 was initially called Beta-adrenergic receptor kinase 2 (βARK-2), and is a member of the G protein-coupled receptor kinase subfamily of the Ser/Thr protein kinases that is most highly similar to GRK2.

References

1 2 3 GRCh38: Ensembl release 89: ENSG00000139436 - Ensembl, May 2017
1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000041890 - Ensembl, May 2017
↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ Premont RT, Claing A, Vitale N, Freeman JL, Pitcher JA, Patton WA, Moss J, Vaughan M, Lefkowitz RJ (Nov 1998). "beta2-Adrenergic receptor regulation by GIT1, a G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein". Proceedings of the National Academy of Sciences of the United States of America. 95 (24): 14082–7. Bibcode:1998PNAS...9514082P. doi: 10.1073/pnas.95.24.14082 . PMC 24330 . PMID 9826657.
↑ Premont RT, Claing A, Vitale N, Perry SJ, Lefkowitz RJ (Jul 2000). "The GIT family of ADP-ribosylation factor GTPase-activating proteins. Functional diversity of GIT2 through alternative splicing". The Journal of Biological Chemistry. 275 (29): 22373–80. doi: 10.1074/jbc.275.29.22373 . PMID 10896954.
1 2 "Entrez Gene: GIT2 G protein-coupled receptor kinase interactor 2".
↑ Kim S, Ko J, Shin H, Lee JR, Lim C, Han JH, Altrock WD, Garner CC, Gundelfinger ED, Premont RT, Kaang BK, Kim E (Feb 2003). "The GIT family of proteins forms multimers and associates with the presynaptic cytomatrix protein Piccolo" (PDF). The Journal of Biological Chemistry. 278 (8): 6291–300. doi: 10.1074/jbc.M212287200 . PMID 12473661.

Nakajima D, Okazaki N, Yamakawa H, Kikuno R, Ohara O, Nagase T (Jun 2002). "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones". DNA Research. 9 (3): 99–106. doi: 10.1093/dnares/9.3.99 . PMID 12168954.
Hoefen RJ, Berk BC (Apr 2006). "The multifunctional GIT family of proteins". Journal of Cell Science. 119 (Pt 8): 1469–75. doi: 10.1242/jcs.02925 . PMID 16598076.
Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Nagase T, Seki N, Tanaka A, Ishikawa K, Nomura N (Aug 1995). "Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1". DNA Research. 2 (4): 167–74, 199–210. doi: 10.1093/dnares/2.4.167 . PMID 8590280.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Turner CE, Brown MC, Perrotta JA, Riedy MC, Nikolopoulos SN, McDonald AR, Bagrodia S, Thomas S, Leventhal PS (May 1999). "Paxillin LD4 motif binds PAK and PIX through a novel 95-kD ankyrin repeat, ARF-GAP protein: A role in cytoskeletal remodeling". The Journal of Cell Biology. 145 (4): 851–63. doi:10.1083/jcb.145.4.851. PMC 2133183 . PMID 10330411.
Bagrodia S, Bailey D, Lenard Z, Hart M, Guan JL, Premont RT, Taylor SJ, Cerione RA (Aug 1999). "A tyrosine-phosphorylated protein that binds to an important regulatory region on the cool family of p21-activated kinase-binding proteins". The Journal of Biological Chemistry. 274 (32): 22393–400. doi: 10.1074/jbc.274.32.22393 . PMID 10428811.
Vitale N, Patton WA, Moss J, Vaughan M, Lefkowitz RJ, Premont RT (May 2000). "GIT proteins, A novel family of phosphatidylinositol 3,4, 5-trisphosphate-stimulated GTPase-activating proteins for ARF6". The Journal of Biological Chemistry. 275 (18): 13901–6. doi: 10.1074/jbc.275.18.13901 . PMID 10788515.
Hoja MR, Wahlestedt C, Höög C (Aug 2000). "A visual intracellular classification strategy for uncharacterized human proteins". Experimental Cell Research. 259 (1): 239–46. doi:10.1006/excr.2000.4948. PMID 10942595.
Ku GM, Yablonski D, Manser E, Lim L, Weiss A (Feb 2001). "A PAK1-PIX-PKL complex is activated by the T-cell receptor independent of Nck, Slp-76 and LAT". The EMBO Journal. 20 (3): 457–65. doi:10.1093/emboj/20.3.457. PMC 133476 . PMID 11157752.
Mazaki Y, Hashimoto S, Okawa K, Tsubouchi A, Nakamura K, Yagi R, Yano H, Kondo A, Iwamatsu A, Mizoguchi A, Sabe H (Mar 2001). "An ADP-ribosylation factor GTPase-activating protein Git2-short/KIAA0148 is involved in subcellular localization of paxillin and actin cytoskeletal organization". Molecular Biology of the Cell. 12 (3): 645–62. doi:10.1091/mbc.12.3.645. PMC 30970 . PMID 11251077.
Kim S, Ko J, Shin H, Lee JR, Lim C, Han JH, Altrock WD, Garner CC, Gundelfinger ED, Premont RT, Kaang BK, Kim E (Feb 2003). "The GIT family of proteins forms multimers and associates with the presynaptic cytomatrix protein Piccolo" (PDF). The Journal of Biological Chemistry. 278 (8): 6291–300. doi: 10.1074/jbc.M212287200 . PMID 12473661.
Brill LM, Salomon AR, Ficarro SB, Mukherji M, Stettler-Gill M, Peters EC (May 2004). "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry". Analytical Chemistry. 76 (10): 2763–72. doi:10.1021/ac035352d. PMID 15144186.
Premont RT, Perry SJ, Schmalzigaug R, Roseman JT, Xing Y, Claing A (Sep 2004). "The GIT/PIX complex: an oligomeric assembly of GIT family ARF GTPase-activating proteins and PIX family Rac1/Cdc42 guanine nucleotide exchange factors". Cellular Signalling. 16 (9): 1001–11. doi:10.1016/j.cellsig.2004.02.002. PMID 15212761.
Jin J, Smith FD, Stark C, Wells CD, Fawcett JP, Kulkarni S, Metalnikov P, O'Donnell P, Taylor P, Taylor L, Zougman A, Woodgett JR, Langeberg LK, Scott JD, Pawson T (Aug 2004). "Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization". Current Biology. 14 (16): 1436–50. doi: 10.1016/j.cub.2004.07.051 . PMID 15324660. S2CID 2371325.

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[refGRCh38Ensembl-1] 1 2 3 GRCh38: Ensembl release 89: ENSG00000139436 - Ensembl, May 2017

[refGRCm38Ensembl-2] 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000041890 - Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid9826657-5] Premont RT, Claing A, Vitale N, Freeman JL, Pitcher JA, Patton WA, Moss J, Vaughan M, Lefkowitz RJ (Nov 1998). "beta2-Adrenergic receptor regulation by GIT1, a G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein". Proceedings of the National Academy of Sciences of the United States of America. 95 (24): 14082–7. Bibcode:1998PNAS...9514082P. doi: 10.1073/pnas.95.24.14082 . PMC 24330 . PMID 9826657.

[pmid10896954-6] Premont RT, Claing A, Vitale N, Perry SJ, Lefkowitz RJ (Jul 2000). "The GIT family of ADP-ribosylation factor GTPase-activating proteins. Functional diversity of GIT2 through alternative splicing". The Journal of Biological Chemistry. 275 (29): 22373–80. doi: 10.1074/jbc.275.29.22373 . PMID 10896954.

[entrez-7] 1 2 "Entrez Gene: GIT2 G protein-coupled receptor kinase interactor 2".

[pmid12473661-8] Kim S, Ko J, Shin H, Lee JR, Lim C, Han JH, Altrock WD, Garner CC, Gundelfinger ED, Premont RT, Kaang BK, Kim E (Feb 2003). "The GIT family of proteins forms multimers and associates with the presynaptic cytomatrix protein Piccolo" (PDF). The Journal of Biological Chemistry. 278 (8): 6291–300. doi: 10.1074/jbc.M212287200 . PMID 12473661.

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GIT2

Contents

Function

Interactions

Related Research Articles

References

Further reading