Gary Glish | |
|---|---|
 | |
| Born | August 23, 1954 |
| Alma mater | Wabash College (B.A.) (1976) Purdue University (Ph.D.) [1] (1980) |
| Scientific career | |
| Fields | Chemistry |
| Institutions | University of North Carolina at Chapel Hill |
| Doctoral advisor | Graham Cooks |
Gary Glish (born August 23, 1954) is an American analytical chemist at the University of North Carolina at Chapel Hill. [1] He is a leading researcher in the fields of mass spectrometry, ion chemistry, and biomolecule analysis.
Gary L. Glish was born on August 23, 1954, in Bay City, Michigan, and grew up in Kalamazoo, Michigan. He received a B.A. in chemistry and a B.A. in economics from Wabash in 1976. [2] He accomplished his Ph.D. in chemistry at Purdue University in 1980. [2] Upon graduating, Glish decided to start a career in analytical chemistry, working with the new technologies afforded by computers to create analytical instrumentation.
Glish designed and built the first ever quadrupole/time of flight (Q/TOF) mass spectrometer [3] and has contributed extensively to the development of the quadrupole ion trap mass spectrometer, including the first interfacing of electrospray ionization [4] and MALDI. [5]
After graduating from Purdue, Glish worked at Oak Ridge National Laboratory as Research Staff and Group Leader from 1980 to 1992. [6] He also served on the Board of Directors for the Asilomar Conference on Mass Spectrometry from 1987 to 1989. He has a long history with the American Society for Mass Spectrometry from 1987 to 2010, first serving as Vice President for Arrangements, then Associate Editor for the Journal of the American Society for Mass Spectrometry, Vice President for Programs, and ultimately President. [6]
Glish started teaching at the University of North Carolina at Chapel Hill in the Department of Chemistry in 1992. [1] He currently leads the Glish Group in research focusing on mass spectrometry analytical methods. [7]
Research by the Glish Group focuses on development and applications of mass spectrometry analytical instrumentation. One of the most prominent technologies used by Glish is differential ion mobility spectrometry, or DIMS. [8] This separation method relies on the differing mobilities of ions in electric fields of varying strengths. [8] DIMS/MS is used by the Glish Group to analyze lipids, leukemia antigen peptides, and aerosol compounds found in e-cigarettes. [6] [9]
Mass spectrometry (MS) is an analytical technique that is used to measure the mass-to-charge ratio of ions. The results are presented as a mass spectrum, a plot of intensity as a function of the mass-to-charge ratio. Mass spectrometry is used in many different fields and is applied to pure samples as well as complex mixtures.
An ion source is a device that creates atomic and molecular ions. Ion sources are used to form ions for mass spectrometers, optical emission spectrometers, particle accelerators, ion implanters and ion engines.
Electrospray ionization (ESI) is a technique used in mass spectrometry to produce ions using an electrospray in which a high voltage is applied to a liquid to create an aerosol. It is especially useful in producing ions from macromolecules because it overcomes the propensity of these molecules to fragment when ionized. ESI is different from other ionization processes since it may produce multiple-charged ions, effectively extending the mass range of the analyser to accommodate the kDa-MDa orders of magnitude observed in proteins and their associated polypeptide fragments.
Tandem mass spectrometry, also known as MS/MS or MS2, is a technique in instrumental analysis where two or more stages of analysis using one or more mass analyzer are performed with an additional reaction step in between these analyses to increase their abilities to analyse chemical samples. A common use of tandem MS is the analysis of biomolecules, such as proteins and peptides.
In mass spectrometry, Orbitrap is an ion trap mass analyzer consisting of an outer barrel-like electrode and a coaxial inner spindle-like electrode that traps ions in an orbital motion around the spindle. The image current from the trapped ions is detected and converted to a mass spectrum using the Fourier transform of the frequency signal.
Thermospray is a soft ionization source by which a solvent flow of liquid sample passes through a very thin heated column to become a spray of fine liquid droplets. As a form of atmospheric pressure ionization in mass spectrometry these droplets are then ionized via a low-current discharge electrode to create a solvent ion plasma. A repeller then directs these charged particles through the skimmer and acceleration region to introduce the aerosolized sample to a mass spectrometer. It is particularly useful in liquid chromatography-mass spectrometry (LC-MS).
Electron-transfer dissociation (ETD) is a method of fragmenting multiply-charged gaseous macromolecules in a mass spectrometer between the stages of tandem mass spectrometry (MS/MS). Similar to electron-capture dissociation, ETD induces fragmentation of large, multiply-charged cations by transferring electrons to them. ETD is used extensively with polymers and biological molecules such as proteins and peptides for sequence analysis. Transferring an electron causes peptide backbone cleavage into c- and z-ions while leaving labile post translational modifications (PTM) intact. The technique only works well for higher charge state peptide or polymer ions (z>2). However, relative to collision-induced dissociation (CID), ETD is advantageous for the fragmentation of longer peptides or even entire proteins. This makes the technique important for top-down proteomics. The method was developed by Hunt and coworkers at the University of Virginia.
Robert Graham Cooks is the Henry Bohn Hass Distinguished Professor of Chemistry in the Aston Laboratories for Mass Spectrometry at Purdue University. He is an ISI Highly Cited Chemist, with over 1,000 publications and an H-index of 144.
Christie G. Enke is a United States academic chemist who made pioneering contributions to the field of analytical chemistry.
Laser spray ionization refers to one of several methods for creating ions using a laser interacting with a spray of neutral particles or ablating material to create a plume of charged particles. The ions thus formed can be separated by m/z with mass spectrometry. Laser spray is one of several ion sources that can be coupled with liquid chromatography-mass spectrometry for the detection of larger molecules.
Ion mobility spectrometry–mass spectrometry (IMS-MS) is an analytical chemistry method that separates gas phase ions based on their interaction with a collision gas and their masses. In the first step, the ions are separated according to their mobility through a buffer gas on a millisecond timescale using an ion mobility spectrometer. The separated ions are then introduced into a mass analyzer in a second step where their mass-to-charge ratios can be determined on a microsecond timescale. The effective separation of analytes achieved with this method makes it widely applicable in the analysis of complex samples such as in proteomics and metabolomics.
A triple quadrupole mass spectrometer (TQMS), is a tandem mass spectrometer consisting of two quadrupole mass analyzers in series, with a (non-mass-resolving) radio frequency (RF)–only quadrupole between them to act as a cell for collision-induced dissociation. This configuration is often abbreviated QqQ, here Q1q2Q3.
A hybrid mass spectrometer is a device for tandem mass spectrometry that consists of a combination of two or more m/z separation devices of different types.
Extractive electrospray ionization (EESI) is a spray-type, ambient ionization source in mass spectrometry that uses two colliding aerosols, one of which is generated by electrospray. In standard EESI, syringe pumps provide the liquids for both an electrospray and a sample spray. In neutral desorption EESI (ND-EESI), the liquid for the sample aerosol is provided by a flow of nitrogen.
Jennifer S. Brodbelt is an American chemist known for her research using mass spectrometry to characterize organic compounds, especially biopolymers and proteins.
John Michael Ramsey is an American analytical chemist at the University of North Carolina at Chapel Hill. He currently holds the position of Minnie N. Goldby Distinguished Professor of Chemistry. His current research with the university focuses on microscale and nanoscale devices such as microchip electrospray, microscale Ion trap mass spectrometers, and microfluidic point of care devices. He is ranked #2 in the "Giants of Nano" field on The Analytical Scientist Power List.
Peter Nemes is a Hungarian-American chemist, who is active in the fields of bioanalytical chemistry, mass spectrometry, cell/developmental biology, neuroscience, and biochemistry.
SCIEX is a manufacturer of mass spectrometry instrumentation used in biomedical and environmental applications. Originally started by scientists from the University of Toronto Institute for Aerospace Studies, it is now part of Danaher Corporation with the SCIEX R&D division still located in Toronto, Canada.
Lidia Nikolaevna Gall was a Russian mass spectrometrist, credited as one of the inventors for electrospray ionization source and high-performance mass analyzers.