Grancalcin

GCA
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1F4O, 1F4Q, 1K94, 1K95
Identifiers
Aliases	GCA , GCL, grancalcin
External IDs	OMIM: 607030 MGI: 1918521 HomoloGene: 22702 GeneCards: GCA
Gene location (Human) Chr. Chromosome 2 (human) [1] Band 2q24.2 Start 162,318,840 bp [1] End 162,371,595 bp [1]
Gene location (Mouse) Chr. Chromosome 2 (mouse) [2] Band 2|2 C1.3 Start 62,494,629 bp [2] End 62,524,453 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in monocyte blood bone marrow bone marrow cells cancellous bone spleen germinal epithelium right lung periodontal fiber corpus callosum Top expressed in morula pineal gland primary motor cortex olfactory epithelium seminal vesicula sciatic nerve retinal pigment epithelium blastocyst Epithelium of stomach myocardium of ventricle More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein binding protein homodimerization activity metal ion binding calcium-dependent cysteine-type endopeptidase activity protein heterodimerization activity calcium ion binding Cellular component plasma membrane extracellular exosome membrane extracellular region cytosol azurophil granule lumen cytoplasm Biological process membrane fusion proteolysis neutrophil degranulation Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 25801 227960 Ensembl ENSG00000115271 ENSMUSG00000026893 UniProt P28676 Q8VC88 RefSeq (mRNA) NM_012198 NM_001330265 NM_001330266 NM_001330267 NM_001330268 NM_001330270 NM_001330271 NM_145523 RefSeq (protein) NP_001317194 NP_001317195 NP_001317196 NP_001317197 NP_001317199 NP_001317200 NP_036330 NP_663498 Location (UCSC) Chr 2: 162.32 – 162.37 Mb Chr 2: 62.49 – 62.52 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Grancalcin is a protein that in humans is encoded by the GCA gene. ^{[5] [6] [7] [8]}

This gene product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation. ^[8]

Interactions

GCA (gene) has been shown to interact with SRI. ^[7]

References

1. GRCh38: Ensembl release 89: ENSG00000115271 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000026893 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Boyhan A, Casimir CM, French JK, Teahan CG, Segal AW (Mar 1992). "Molecular cloning and characterization of grancalcin, a novel EF-hand calcium-binding protein abundant in neutrophils and monocytes". J Biol Chem. 267 (5): 2928–33. doi: 10.1016/S0021-9258(19)50675-5 . PMID   1737748.
6. Teahan CG, Totty NF, Segal AW (Oct 1992). "Isolation and characterization of grancalcin, a novel 28 kDa EF-hand calcium-binding protein from human neutrophils". Biochem J. 286. ( Pt 2) (2): 549–54. doi:10.1042/bj2860549. PMC   1132932 . PMID   1530588.
7. Hansen C, Tarabykina S, la Cour JM, Lollike K, Berchtold MW (Jun 2003). "The PEF family proteins sorcin and grancalcin interact in vivo and in vitro". FEBS Lett. 545 (2–3): 151–4. doi: 10.1016/S0014-5793(03)00518-0 . PMID   12804766. S2CID   42572647.
8. "Entrez Gene: GCA grancalcin, EF-hand calcium binding protein".

Further reading

• Lim J, Hao T, Shaw C, et al. (2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell. 125 (4): 801–14. doi: 10.1016/j.cell.2006.03.032 . PMID   16713569. S2CID   13709685.
• Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID   16189514. S2CID   4427026.
• Hillier LW, Graves TA, Fulton RS, et al. (2005). "Generation and annotation of the DNA sequences of human chromosomes 2 and 4". Nature. 434 (7034): 724–31. Bibcode:2005Natur.434..724H. doi: 10.1038/nature03466 . PMID   15815621.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC   528928 . PMID   15489334.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Jia J, Borregaard N, Lollike K, Cygler M (2002). "Structure of Ca(2+)-loaded human grancalcin". Acta Crystallogr. D. 57 (Pt 12): 1843–9. doi: 10.1107/S0907444901016511 . PMID   11717497.
• Lollike K, Johnsen AH, Durussel I, et al. (2001). "Biochemical characterization of the penta-EF-hand protein grancalcin and identification of L-plastin as a binding partner". J. Biol. Chem. 276 (21): 17762–9. doi: 10.1074/jbc.M100965200 . PMID   11279160.
• Jia J, Han Q, Borregaard N, et al. (2000). "Crystal structure of human grancalcin, a member of the penta-EF-hand protein family". J. Mol. Biol. 300 (5): 1271–81. doi:10.1006/jmbi.2000.3925. PMID   10903868.
• Maki M, Narayana SV, Hitomi K (1998). "A growing family of the Ca2+-binding proteins with five EF-hand motifs". Biochem. J. 328. ( Pt 2): 718–20. PMC   1218977 . PMID   9441591.