| Actor | Character | Description | Season 1 | Season 2 |
|---|
| Deepa Balu | Dr.Reena Vijay | A compassionate and resilient surgical intern (now senior resident) who is the estranged daughter of a renowned surgeons, navigating her career while trying to reconnect with her parents and complexities of their strained relationship. She often finds herself in trouble for prioritizing her patients' well-being over strict rules. | Main |
| Kanishka Vijaykumar Y | Guest |
| Anumol | Dr.Radhi Thiyagarajan | A strict and workaholic chief surgeon known for her professional brilliance, who struggles to balance her career with a complicated personal life and a strained relationship with her past. | Main |
| Sachana Namidass Y | | Guest |
| Charukesh | Arjun | The supportive and loving son of the hospital's founder, who becomes the CEO and Reena's love interest, navigating professional challenges while being a steadfast presence in her life. | Main |
| Karthik Kumar | Dr.Vijay | Reena's biological father and Radhi's former lover, whose unexpected return adds a layer of complexity and drama to the lives of the two main characters. | Mentioned | Main |
| Roshan A Y | | Guest |
| Mohan Raman | Thiyagarajan / "Thayagu" | Radhi's father | Guest | Main |
| Sabaresh | Dr.Ramakrishna "Rocky" | A lighthearted and humorous medical intern who provides a comedic touch and much-needed relief to the intense atmosphere of the hospital. | Main |
| Yogalakshmi | Dr.Tejasri "Teju" | A relatable medical intern and a close friend to Reena, who struggles with the immense pressure of her residency while trying to maintain a normal personal life. | Main |
| RG Ram | Dr.Naveen | A fellow medical intern. He is often part of the group of interns, navigating the challenges of their residency and personal lives. | Main |
| T. M. Karthik | Dr.Preetham | A manipulative and ill-willed doctor who tries to create discord among the hospital staff to usurp the CEO's position. | | Main |
| Amit Bhargav | Madhan | A recurring character who is always present and often adds to the drama. His character is noted for not giving off "villain vibes" despite being one of the more straight-up antagonists in the show. | Main | Recurring |
| Jayarao | RK | The chairman and founder of RK Multispeciality Hospital and Arjun's father. He is a key figure in the hospital's power dynamics and is concerned about his son's reckless behavior, hoping a good marriage will bring him stability. His declining health becomes a central point of drama in the series. | Main | Mentioned |
| Gurulakshman | Dr.Ravi | A senior intern at the hospital. He is often seen alongside fellow senior intern Dr. Anitha, and their close relationship is a topic of speculation among the other interns. He is a dedicated professional but can be emotionally vulnerable, as shown when he freezes during a critical surgery. | Main | Mentioned |
| Padine Kumar | Dr.Anitha | Another senior medical intern. She is a versatile and dedicated professional who often works closely with Dr. Ravi. Her character goes through a "rollercoaster" of emotional shifts throughout the series. | Main |
| Sharmila Thapa | Aruna | A senior nurse in RK Hospital | Main |
| Sarvhaa | Dr.Guna | Another medical intern who is a close friend of Reena and Rocky. He is depicted as a supportive and reliable member of the intern group. | Main |
| Chandrasekar Koneru | Dev | Radhi's husband | Main |
| Diyansh K | Sid | Radhi and Dev's son | Main |
| Riya Manoj | Divya "Divi" | Radhi and Dev's daughter | Main |
| Ashwathy Agnihothri | Manasa | a psychologist; She returns and informs Madhan (one of her brother) that she wants the hospital back, suggesting she has a past connection to the hospital and an agenda of her own. | Mentioned | Main |
| Akshathaa Ajit | Dr.Nilofer | A new medical intern who joins RK Multispeciality Hospital in the second season. She is a dedicated young doctor whose personal life, including an engagement, becomes a part of the series' storyline. | | Main |
| Shivaraman M | Dr.Kiran | A new medical intern who joins the hospital in the second season. He is depicted as being interested in his colleague, Nilofer. | | Main |
| Abdool Lee | Dr.Kamalakannan | A doctor at the hospital who gets confused by Amaya's twin sister, Abinaya, leading to a key moment where Amaya's lies are exposed. | | Main |
| Roshni | Amaya | One of the nurses in RK Hospital and a mysterious character with a hidden identity, whose lies about having a twin sister are eventually exposed, causing major plot twists. | | Main |
| Abinaya | an Unemployed; Amaya's twin | | Recurring |
| Kavithalayaa Krishnan | Ganesh | Thayagu's friend and Radhi's neighbor who adopted Reena as his daughter | Main |
| Devishree | Sharadha | Ganesh's wife | Main |
| Sindhu Shyam | Jennifer | A Classical dancer; Suryakant's ex-wife; Guna's mother | Recurring |
| Giri Dwarakish | Dr.Ramanathan "Cinema Doctor" | An amusing character known for his love of cinema. He is a senior figure at the hospital who provides a lighter, more entertaining dynamic to the series. | Main | Recurring |
| Syamantha Kiran | Suchitra Madhan "Suchi" | Madhan's wife | Recurring |
| Varshini Suresh | Sonia | Reena's best friend since childhood who settled in Kolkata by marriage | Recurring |
| Sherin Farhana Y | Guest |
| Samridha Sam | Agalya Madhan | Madhan and Suchitra's daughter | Recurring |
| Riyaz Khan | Suryakant | An actor; Jennifer's husband and Guna's father | Guest |
| Aruljothi Arockiaraj | Charu | Ramanathan's daughter; Rocky's love interest | Recurring |
| Revathi Elango | Bharathi | Ravi's cousin turned wife | Main | |
| Gaayathri Krishnan | Rani | Ravi's mother | Recurring | |
| Bose Venkat | – | | Guest | |
| Saravanan Rajavel | — | | Guest | |
| Yogi | Murugan | Reena's hostel warden | Guest | |
| Dasarathy | MNM | RK's business-rival; Father of Madhan, Manasa and Naveen | Guest | Mentioned |
| Indumathy Manikandan | Pallavi | RK's wife and Arjun's mother | Guest | Mentioned |
