In vitro compartmentalization

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In vitro compartmentalization (IVC) is an emulsion-based technology that generates cell-like compartments in vitro . These compartments are designed such that each contains no more than one gene. When the gene is transcribed and/or translated, its products (RNAs and/or proteins) become 'trapped' with the encoding gene inside the compartment. By coupling the genotype (DNA) and phenotype (RNA, protein), compartmentalization allows the selection and evolution of phenotype.

Contents

History

In vitro compartmentalization method was first developed by Dan Tawfik and Andrew Griffiths. [1] Based on the idea that Darwinian evolution relies on the linkage of genotype to phenotype, Tawfik and Griffiths designed aqueous compartments of water-in-oil (w/o) emulsions to mimic cellular compartments that can link genotype and phenotype. Emulsions of cell-like compartments were formed by adding in vitro transcription/translation reaction mixture to stirred mineral oil containing surfactants. The mean droplet diameter was measured to be 2.6 μm by laser diffraction. As a proof of concept, Tawfik and Griffiths designed a selection experiment using a pool of DNA sequences, including the gene encoding HaeIII DNA methyltransferase (M.HaeIII) in the presence of 107-fold excess of genes encoding a different enzyme folA. The 3’ of each DNA sequences was purposely designed to contain a HaeIII recognition site which, in the presence of expressed methyltransferase, would be methylated and, thus, resistant to restriction enzyme digestion. By selecting for DNA sequences that survive the endonuclease digestion, Tawfik and Griffiths found that the M.HaeIII genes were enriched by at least 1000-fold over the folA genes within the first round of selection.

Method

Comparison of in vivo cell compartmentalisation (left) versus in vitro compartmentalization (right) using double emulsion droplets and fluorescent sorting. 1) In an in vivo system, a library of genes is transformed into bacteria to produce the protein variants and dispersed into a water-in-oil (w/o) emulsion aiming to have each droplet contain maximum one cell. 2) In an in vitro system, no cells are used and instead protein variants are produced via in vitro transcription/translation aiming to have one gene per droplet. 3,4) In both cases, droplets are then resuspended in water to make a water-in-oil-in-water (w/o/w) emulsion, have a fluorogenic substrate added, and passed in front of the laser and detector in a fluorescence activated cell sorter. This causes genes that encode active protein variants to be isolated and amplified, sequenced and used for further rounds of selection or directed evolution. Double emulsion in vitro compartmentalization.gif
Comparison of in vivo cell compartmentalisation (left) versus in vitro compartmentalization (right) using double emulsion droplets and fluorescent sorting.
1) In an in vivo system, a library of genes is transformed into bacteria to produce the protein variants and dispersed into a water‐in‐oil (w/o) emulsion aiming to have each droplet contain maximum one cell.
2) In an in vitro system, no cells are used and instead protein variants are produced via in vitro transcription/translation aiming to have one gene per droplet.
3,4) In both cases, droplets are then resuspended in water to make a water‐in‐oil‐in‐water (w/o/w) emulsion, have a fluorogenic substrate added, and passed in front of the laser and detector in a fluorescence activated cell sorter. This causes genes that encode active protein variants to be isolated and amplified, sequenced and used for further rounds of selection or directed evolution.

Emulsion technology

Water-in-oil (w/o) emulsions are created by mixing aqueous and oil phases with the help of surfactants. A typical IVC emulsion is formed by first generating oil-surfactant mixture by stirring, and then gradually adding the aqueous phase to the oil-surfactant mixture. For stable emulsion formation, a mixture of HLB (hydrophile-lipophile balance) and low HLB surfactants are needed. [3] Some combinations of surfactants used to generate oil-surfactant mixture are mineral oil / 0.5% Tween 80 / 4.5% Span 80 / sodium deoxycholate [1] and a more heat stable version, light mineral oil / 0.4% Tween 80 / 4.5% Span 80 / 0.05% Triton X-100. [4] The aqueous phase containing transcription and/or translation components is slowly added to the oil surfactants, and the formation of w/o is facilitated by homogenizing, stirring or using hand extruding device.

The emulsion quality can be determined by light microscopy and/or dynamic light scattering techniques. The emulsion is quite diverse, and greater homogenization speeds helps to produce smaller droplets with narrower size distribution. However, homogenization speeds has to be controlled, since speed over 13,500 r.p.m tends to result in a significant loss of enzyme activity on the level of transcription. The most widely used emulsion formation gives droplets with a mean diameter of 2-3μm, and an average volume of ~5 femtoliters, or 1010 aqueous droplet per ml of emulsions. [5] The ratio of genes to droplets is designed such that most of the droplets contains no more than a single gene statistically.

In vitro transcription/translation

IVC enables the miniaturization of large-scale techniques that can now be done on the micro scale including coupled in vitro transcription and translation (IVTT) experiments. Streamlining and integrating transcription and translation allows for fast and highly controllable experimental designs. [6] [7] [8] IVTT can be done both in bulk emulsions and in microdroplets by utilizing droplet-based microfluidics. Microdroplets, droplets on the scale of pico to femtoliters, have been successfully used as single DNA molecule vessels. [9] [10] This droplet technology allows high throughput analysis with many different selection pressures in a single experimental setup. [6] [10] IVTT in microdroplets is preferred when overexpression of a desired protein would be toxic to a host cell minimizing the utility of the transcription and translation mechanisms. [11]

IVC has used bacterial cell, wheat germ and rabbit reticulocyte (RRL) extracts for transcription and translation. It is also possible to use bacterial reconstituted translation system such as PURE in which translation components are individually purified and later combined. When expressing eukaryote or complex proteins, it is desirable to use eukaryotic translation systems such as wheat germ extract or more superior alternative, RRL extract. In order to use RRL for transcription and translation, traditional emulsion formulation cannot be used as it abolishes translation. Instead, a novel emulsion formulation: 4% Abil EM90 / light mineral oil was developed and demonstrated to be functional in expressing luciferase and human telomerase. [12]

Breaking emulsion and coupling of genotype and phenotype

Once transcription and/or translation has completed in the droplets, emulsion will be broken by successive steps of removing mineral oil and surfactants to allow for subsequent selection. At this stage, it is crucial to have a method to ‘track’ each gene products to the encoding gene as they become free floating in a heterogeneous population of molecules. There are three major approaches to track down each phenotype to its genotype. [13] The first method is to attach each DNA molecule with a biotin group and an additional coding sequence for streptavidin (STABLE display). [14] All the newly formed proteins/peptides will be in fusion with streptavidin molecules and bind to their biotinylated coding sequence. An improved version attached two biotin molecules to the ends of a DNA molecule to increase the avidity between DNA molecule and streptavidin-fused peptides, and used a low GC content synthetic streptavidin gene to increase efficiency and specificity during PCR amplification. [15] The second method is to covalently link DNA and protein. Two strategies have been demonstrated. The first is to form M.HaeIII fusion proteins. [16] Each expressed protein/polypeptide will be in fusion with Hae III DNA methyltransferase domain, which is able to bind covalently to DNA fragments containing the sequence 5′-GGC*-3′, where C* is 5-fluoro-2 deoxycytidine. The second strategy is to use monomeric mutant of VirD2 enzyme. [17] When a protein/peptide is expressed in fusion with Agrobacterium protein VirD2, it will bind to its DNA coding sequence that has a single-stranded overhang comprising VirD2 T-border recognition sequences. The third method is to link phenotype and genotype via beads. [18] The beads used will be coated with streptavidin to allow for the binding of biotinylated DNA, in addition, the beads will also display cognate binding partner to the affinity tag that will be expressed in fusion with the protein/peptide.

Selection

Depending on the phenotype to be selected, difference selection strategies will be used. Selection strategy can be divided into three major categories: selection for binding, selection for catalysis and selection for regulation. [19] The phenotype to be selected can range from RNA to peptide to protein. By selecting for binding, the most commonly evolved phenotypes are peptide/proteins that have selective affinity to a specific antibody or DNA molecule. An example is the selection of proteins that have affinity to zinc finger DNA by Sepp et al. [20] By selecting for catalytic proteins/RNAs, new variants with novel or improved enzymatic property are usually isolated. For example, new ribozyme variants with trans-ligase activity were selected and exhibited multiple turnovers. [21] By selecting for regulation, inhibitors of DNA nucleases can be selected, such as protein inhibitors of the Colicin E7 DNase. [22]

Advantages

Comparing to other in vitro display technologies, IVC has two major advantages. The first advantage is its ability to control reactions within the droplets. Hydrophobic and hydrophilic components can be delivered to each droplet in a step-wise fashion without compromising the chemical integrity of the droplet, and thus by controlling what to be added and when to be added, the reaction in each droplet is controlled. In addition, depending on the nature of the reaction to be carried out, the pH of each droplet can also be changed. More recently, photocaged substrates were used and their participation in a reaction was regulated by photo-activation. [19] The second advantage is that IVC allows the selection of catalytic molecules. As an example, Griffiths et al. was able to select for phosphotriesterase variants with higher Kcat by detecting product formation and amount using anti-product antibody and flow cytometry respectively. [23]

Related Research Articles

An emulsion is a mixture of two or more liquids that are normally immiscible owing to liquid-liquid phase separation. Emulsions are part of a more general class of two-phase systems of matter called colloids. Although the terms colloid and emulsion are sometimes used interchangeably, emulsion should be used when both phases, dispersed and continuous, are liquids. In an emulsion, one liquid is dispersed in the other. Examples of emulsions include vinaigrettes, homogenized milk, liquid biomolecular condensates, and some cutting fluids for metal working.

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<span class="mw-page-title-main">Gene expression</span> Conversion of a genes sequence into a mature gene product or products

Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product that enables it to produce end products, proteins or non-coding RNA, and ultimately affect a phenotype. These products are often proteins, but in non-protein-coding genes such as transfer RNA (tRNA) and small nuclear RNA (snRNA), the product is a functional non-coding RNA. Gene expression is summarized in the central dogma of molecular biology first formulated by Francis Crick in 1958, further developed in his 1970 article, and expanded by the subsequent discoveries of reverse transcription and RNA replication.

<span class="mw-page-title-main">Morpholino</span> Chemical compound

A Morpholino, also known as a Morpholino oligomer and as a phosphorodiamidate Morpholino oligomer (PMO), is a type of oligomer molecule used in molecular biology to modify gene expression. Its molecular structure contains DNA bases attached to a backbone of methylenemorpholine rings linked through phosphorodiamidate groups. Morpholinos block access of other molecules to small specific sequences of the base-pairing surfaces of ribonucleic acid (RNA). Morpholinos are used as research tools for reverse genetics by knocking down gene function.

<span class="mw-page-title-main">Phage display</span> Biological technique to evolve proteins using bacteriophages

Phage display is a laboratory technique for the study of protein–protein, protein–peptide, and protein–DNA interactions that uses bacteriophages to connect proteins with the genetic information that encodes them. In this technique, a gene encoding a protein of interest is inserted into a phage coat protein gene, causing the phage to "display" the protein on its outside while containing the gene for the protein on its inside, resulting in a connection between genotype and phenotype. The proteins that the phages are displaying can then be screened against other proteins, peptides or DNA sequences, in order to detect interaction between the displayed protein and those of other molecules. In this way, large libraries of proteins can be screened and amplified in a process called in vitro selection, which is analogous to natural selection.

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mRNA display

mRNA display is a display technique used for in vitro protein, and/or peptide evolution to create molecules that can bind to a desired target. The process results in translated peptides or proteins that are associated with their mRNA progenitor via a puromycin linkage. The complex then binds to an immobilized target in a selection step. The mRNA-protein fusions that bind well are then reverse transcribed to cDNA and their sequence amplified via a polymerase chain reaction. The result is a nucleotide sequence that encodes a peptide with high affinity for the molecule of interest.

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References

  1. 1 2 Tawfik DS, Griffiths AD (July 1998). "Man-made cell-like compartments for molecular evolution". Nature Biotechnology. 16 (7): 652–6. doi:10.1038/nbt0798-652. PMID   9661199. S2CID   25527137.
  2. ↑ Ferrer M, Beloqui A, Vieites JM, Guazzaroni ME, Berger I, Aharoni A (January 2009). "Interplay of metagenomics and in vitro compartmentalization". Microbial Biotechnology. 2 (1): 31–9. doi:10.1111/j.1751-7915.2008.00057.x. PMC   3815420 . PMID   21261880.
  3. ↑ Rothe A, Surjadi RN, Power BE (December 2006). "Novel proteins in emulsions using in vitro compartmentalization". Trends in Biotechnology. 24 (12): 587–92. doi:10.1016/j.tibtech.2006.10.007. PMID   17055094.
  4. ↑ Ghadessy FJ, Ong JL, Holliger P (April 2001). "Directed evolution of polymerase function by compartmentalized self-replication". Proceedings of the National Academy of Sciences of the United States of America. 98 (8): 4552–7. Bibcode:2001PNAS...98.4552G. doi: 10.1073/pnas.071052198 . PMC   31872 . PMID   11274352.
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  6. 1 2 Castro-Roa D, Zenkin N (September 2015). "Methodology for the analysis of transcription and translation in transcription-coupled-to-translation systems in vitro". Methods. 86: 51–9. doi: 10.1016/j.ymeth.2015.05.029 . PMID   26080048.
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  10. 1 2 Courtois F, Olguin LF, Whyte G, Bratton D, Huck WT, Abell C, Hollfelder F (February 2008). "An integrated device for monitoring time-dependent in vitro expression from single genes in picolitre droplets". ChemBioChem. 9 (3): 439–46. doi:10.1002/cbic.200700536. PMID   18232037. S2CID   8684268.
  11. ↑ Colin PY, Zinchenko A, Hollfelder F (August 2015). "Enzyme engineering in biomimetic compartments". Current Opinion in Structural Biology. 33: 42–51. doi:10.1016/j.sbi.2015.06.001. PMID   26311177.
  12. ↑ Ghadessy FJ, Holliger P (March 2004). "A novel emulsion mixture for in vitro compartmentalization of transcription and translation in the rabbit reticulocyte system". Protein Engineering, Design & Selection. 17 (3): 201–4. doi: 10.1093/protein/gzh025 . PMID   14990785.
  13. ↑ Leemhuis H, Stein V, Griffiths AD, Hollfelder F (August 2005). "New genotype–phenotype linkages for directed evolution of functional proteins". Current Opinion in Structural Biology. 15 (4): 472–8. doi:10.1016/j.sbi.2005.07.006. PMID   16043338.
  14. ↑ Yonezawa M, Doi N, Higashinakagawa T, Yanagawa H (March 2004). "DNA display of biologically active proteins for in vitro protein selection". Journal of Biochemistry. 135 (3): 285–8. doi:10.1093/jb/mvh034. PMID   15113826.
  15. ↑ Yonezawa M, Doi N, Kawahashi Y, Higashinakagawa T, Yanagawa H (October 2003). "DNA display for in vitro selection of diverse peptide libraries". Nucleic Acids Research. 31 (19): 118e–118. doi:10.1093/nar/gng119. PMC   206484 . PMID   14500846.
  16. ↑ Bertschinger J, Neri D (September 2004). "Covalent DNA display as a novel tool for directed evolution of proteins in vitro". Protein Engineering, Design & Selection. 17 (9): 699–707. doi: 10.1093/protein/gzh082 . hdl: 20.500.11850/34052 . PMID   15522920.
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  19. 1 2 Griffiths AD, Tawfik DS (September 2006). "Miniaturising the laboratory in emulsion droplets". Trends in Biotechnology. 24 (9): 395–402. doi:10.1016/j.tibtech.2006.06.009. PMID   16843558.
  20. ↑ Sepp A, Choo Y (November 2005). "Cell-free selection of zinc finger DNA-binding proteins using in vitro compartmentalization". Journal of Molecular Biology. 354 (2): 212–9. doi:10.1016/j.jmb.2005.09.051. PMID   16242713.
  21. ↑ Levy M, Griswold KE, Ellington AD (October 2005). "Direct selection of trans-acting ligase ribozymes by in vitro compartmentalization". RNA. 11 (10): 1555–62. doi:10.1261/rna.2121705. PMC   1370839 . PMID   16131588.
  22. ↑ Bernath K, Magdassi S, Tawfik DS (February 2005). "Directed evolution of protein inhibitors of DNA-nucleases by in vitro compartmentalization (IVC) and nano-droplet delivery". Journal of Molecular Biology. 345 (5): 1015–26. doi:10.1016/j.jmb.2004.11.017. PMID   15644201.
  23. ↑ Griffiths AD, Tawfik DS (January 2003). "Directed evolution of an extremely fast phosphotriesterase by in vitro compartmentalization". The EMBO Journal. 22 (1): 24–35. doi:10.1093/emboj/cdg014. PMC   140064 . PMID   12505981.
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