Triton X-100

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Triton X-100
Triton X-100.svg
IUPAC name
Other names
  • Polyethylene glycol p-(1,1,3,3-tetramethylbutyl)-phenyl ether
  • Octyl phenol ethoxylate
  • Polyoxyethylene octyl phenyl ether
  • 4-Octylphenol polyethoxylate
  • Mono 30
  • TX-100
  • t-Octylphenoxypolyethoxyethanol
  • Octoxynol-9
3D model (JSmol)
ECHA InfoCard 100.123.919 OOjs UI icon edit-ltr-progressive.svg
PubChem CID
  • CC(C)(C)CC(C)(C)C1=CC=C(C=C1)OCCO
n (n = 9-10)
Molar mass 647 g mol−1
Appearanceviscous colourless liquid
Density 1.07 g/cm3
Melting point 6 °C (43 °F; 279 K)
Boiling point 270 °C (518 °F; 543 K)
Surface tension:
0.22 mM [1]
Vapor pressure < 1 mmHg (130 Pa) at 20 °C
1.490-1.494 [2]
Flash point 251 °C (484 °F; 524 K)
Safety data sheet (SDS) MSDS
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Yes check.svgY  verify  (what is  Yes check.svgYX mark.svgN ?)

Triton X-100 (C
n) is a nonionic surfactant that has a hydrophilic polyethylene oxide chain (on average it has 9.5 ethylene oxide units) and an aromatic hydrocarbon lipophilic or hydrophobic group. The hydrocarbon group is a 4-(1,1,3,3-tetramethylbutyl)-phenyl group. Triton X-100 is closely related to IGEPAL CA-630 or former Nonidet P-40, which might differ from it mainly in having slightly shorter ethylene oxide chains. Thus Triton X-100 is slightly more hydrophilic than Igepal CA-630; these two detergents may not be considered to be functionally interchangeable for most applications. [3]


Triton X-100 was originally a registered trademark of Rohm & Haas Co. It was subsequently purchased by Union Carbide and then acquired by Dow Chemical Company upon the acquisition of Union Carbide. Soon afterward (in 2009), Dow also acquired Rohm & Haas Co.

Physical properties

Undiluted Triton X-100 is a clear viscous fluid (less viscous than undiluted glycerol). Undiluted Triton X-100 has a viscosity of about 270 centipoise at 25 °C which comes down to about 80 centipoise at 50 °C. Triton X-100 is soluble at 25 °C in water, toluene, xylene, trichloroethylene, ethylene glycol, ethyl ether, ethyl alcohol, isopropyl alcohol, and ethylene dichloride. Triton X-100 is insoluble in kerosene, mineral spirits, and naphtha, unless a coupling agent like oleic acid is used. [4]


Triton X-100 is a commonly used detergent in laboratories. [5] Triton X-100 is widely used to lyse cells to extract protein or organelles, or to permeabilize the membranes of living cells. [6]

Some applications include:

Apart from laboratory use, Triton X-100 can be found in several types of cleaning compounds, [7] ranging from heavy-duty industrial products to gentle detergents. It is also a popular ingredient in homemade vinyl record cleaning fluids together with distilled water and isopropyl alcohol. [8]

Regulation in the European Union

In December 2012, the European Chemicals Agency (ECHA) included the substance group “4-(1,1,3,3-tetramethylbutyl)phenol, ethoxylated” – which includes Triton X-100 – in the Candidate List of substances of very high concern [9] of the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) Regulation which addresses the production, import and use of chemical substances and their potential impacts on human health and the environment. [10] A Triton X-100 degradation product has indeed turned out to be ecotoxic as it possesses hormone-like (estrogeno-mimetic) activity that may act on wildlife. [11] The ECHA finally included the substance group in the Authorisation List (Annex XIV), [12] mandating the pharmaceutical and other industries to replace this detergent by the “sunset date” January 4, 2021, thereby affecting EU manufacturers, importers, and downstream users, as well as non-European manufacturers exporting their products into the EU.

Alternatives for viral inactivation

Since the inclusion of Triton X-100 in the candidate list of substances of very high concern for authorization, pharmaceutical companies, as well as bioprocessing research groups, are in need of an alternative detergent which must at the same time be eco-friendly and effective. Ideally, a Triton X-100 replacement should generate minimal manufacturing process change, because only then the necessary updates of regulatory filings for medicines could be realized without additional animal experiments or even clinical studies. Therefore, an alternative virus-inactivating detergent should have physico-chemical properties similar to Triton X-100, should be soluble, easy to remove, eco-friendly, but not degrade to toxic metabolites. In a recent study, [13] two alternatives for antiviral treatment in biopharmaceutical manufacturing have been identified: Triton X-100 reduced, as well as a novel compound which was named Nereid (after the mermaids in Greek mythology). As reflected by the name, Nereid can be seen as just another relative of the Triton X-100 family, however, due to a small molecular difference, it does not degrade into phenolic compounds the way that Triton X-100 does. The virus inactivation studies comprised experiments with several relevant viruses under various conditions. It turned out that at room temperature, where most virus inactivation steps in biopharmaceutical manufacturing are conducted, both Triton X-100 reduced and Nereid showed similar virus inactivating performances as Triton X-100. In contrast, for some processes that are conducted at cold temperatures, Nereid and Triton X-100 gave better results than Triton X-100 reduced.

Related Research Articles

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  10. "REACH Legislation". European Chemicals Agency. An agency of the European Union. Retrieved December 14, 2019.
  11. White, R.; Jobling, S.; Hoare, S. A.; Sumpter, J. P.; Parker, M. G. (1 July 1994). "Environmentally persistent alkylphenolic compounds are estrogenic". Endocrinology. 135 (1): 175–182. doi: 10.1210/endo.135.1.8013351 . PMID   8013351.
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