Agrobacterium tumefaciens is the causal agent of crown gall disease in over 140 species of eudicots. It is a rod-shaped, Gram-negative soil bacterium. Symptoms are caused by the insertion of a small segment of DNA, from a plasmid into the plant cell, which is incorporated at a semi-random location into the plant genome. Plant genomes can be engineered by use of Agrobacterium for the delivery of sequences hosted in T-DNA binary vectors.
Agrobacterium is a genus of Gram-negative bacteria established by H. J. Conn that uses horizontal gene transfer to cause tumors in plants. Agrobacterium tumefaciens is the most commonly studied species in this genus. Agrobacterium is well known for its ability to transfer DNA between itself and plants, and for this reason it has become an important tool for genetic engineering.
The transfer DNA is the transferred DNA of the tumor-inducing (Ti) plasmid of some species of bacteria such as Agrobacterium tumefaciens and Agrobacterium rhizogenes(actually an Ri plasmid). The T-DNA is transferred from bacterium into the host plant's nuclear DNA genome. The capability of this specialized tumor-inducing (Ti) plasmid is attributed to two essential regions required for DNA transfer to the host cell. The T-DNA is bordered by 25-base-pair repeats on each end. Transfer is initiated at the right border and terminated at the left border and requires the vir genes of the Ti plasmid.
Mary-Dell Chilton is one of the founders of modern plant biotechnology.
A tumour inducing (Ti) plasmid is a plasmid found in pathogenic species of Agrobacterium, including A. tumefaciens, A. rhizogenes, A. rubi and A. vitis.
Genetically modified crops are plants used in agriculture, the DNA of which has been modified using genetic engineering methods. Plant genomes can be engineered by physical methods or by use of Agrobacterium for the delivery of sequences hosted in T-DNA binary vectors. In most cases, the aim is to introduce a new trait to the plant which does not occur naturally in the species. Examples in food crops include resistance to certain pests, diseases, environmental conditions, reduction of spoilage, resistance to chemical treatments, or improving the nutrient profile of the crop. Examples in non-food crops include production of pharmaceutical agents, biofuels, and other industrially useful goods, as well as for bioremediation.
Gene delivery is the process of introducing foreign genetic material, such as DNA or RNA, into host cells. Gene delivery must reach the genome of the host cell to induce gene expression. Successful gene delivery requires the foreign gene delivery to remain stable within the host cell and can either integrate into the genome or replicate independently of it. This requires foreign DNA to be synthesized as part of a vector, which is designed to enter the desired host cell and deliver the transgene to that cell's genome. Vectors utilized as the method for gene delivery can be divided into two categories, recombinant viruses and synthetic vectors.
Jozef Stefaan "Jeff", Baron Schell was a Belgian molecular biologist.
A transfer DNA (T-DNA) binary system is a pair of plasmids consisting of a T-DNA binary vector and a virhelper plasmid. The two plasmids are used together to produce genetically modified plants. They are artificial vectors that have been derived from the naturally occurring Ti plasmid found in bacterial species of the genus Agrobacterium, such as A. tumefaciens. The binary vector is a shuttle vector, so-called because it is able to replicate in multiple hosts.
Chad Alexander Mirkin is an American chemist. He is the George B. Rathmann professor of chemistry, professor of medicine, professor of materials science and engineering, professor of biomedical engineering, and professor of chemical and biological engineering, and director of the International Institute for Nanotechnology and Center for Nanofabrication and Molecular Self-Assembly at Northwestern University.
Plant genetics is the study of genes, genetic variation, and heredity specifically in plants. It is generally considered a field of biology and botany, but intersects frequently with many other life sciences and is strongly linked with the study of information systems. Plant genetics is similar in many ways to animal genetics but differs in a few key areas.
Paula Therese Hammond is a David H. Koch Professor in Engineering and the Head of the Department of Chemical Engineering at the Massachusetts Institute of Technology (MIT). Her laboratory designs polymers and nanoparticles for drug delivery and energy-related applications including batteries and fuel cells. She is an intramural faculty member of the Koch Institute for Integrative Cancer Research and an Associate Editor of ACS Nano.
Barbara HohnForMemRS is an Austrian molecular biologist, particularly known for her research into the Agrobacterium tumefaciens.
Yamuna Krishnan is a professor at the Department of Chemistry, University of Chicago, where she has worked since August 2014. She was born to P.T. Krishnan and Mini in Parappanangadi, in the Malappuram district of Kerala, India. She was earlier a Reader in National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India. Krishnan won the Shanti Swarup Bhatnagar Prize for science and technology, the highest science award in India in the year 2013 in the Chemical Science category.
Robert Thomas Fraley was Executive Vice President and Chief Technology Officer at Monsanto, where he helped to develop the first genetically modified seeds. He retired from Monsanto in June 2018. He advocates for the use of GMO products to address global food insecurity and reduce the environmental footprint of agriculture.
Christy Lynn Haynes is a chemist at the University of Minnesota. She works at the interface of analytical, biological, and nanomaterials chemistry.
Research has shown nanoparticles to be a groundbreaking tool for tackling many arising global issues, the agricultural industry being no exception. In general, a nanoparticle is defined as any particle where one characteristic dimension is 100nm or less. Because of their unique size, these particles begin to exhibit properties that their larger counterparts may not. Due to their scale, quantum mechanical interactions become more important than classic mechanical forces, allowing for the prevalence of unique physical and chemical properties due to their extremely high surface-to-body ratio. Properties such as cation exchange capacity, enhanced diffusion, ion adsorption, and complexation are enhanced when operating at nanoscale.
Elizabeth E. Hood is a plant geneticist and the Lipscomb Distinguished Professor of Agriculture at Arkansas State University. In 2018 she was elected a fellow of the American Association for the Advancement of Science.
Alice Cheung is an American biochemist who is a Professor of Molecular Biology at the University of Massachusetts Amherst. Her research considers the molecular and cellular biology of polarization. She was elected a Fellow of the American Association for the Advancement of Science in 2020.
Rizia Bardhan is an American biomolecular engineer who is an Associate Professor of Chemical & Biological Engineering at Iowa State University. She is Associate Editor of ACS Applied Materials & Interfaces.
