MCM3AP

MCM3AP
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4DHX
Identifiers
Aliases	MCM3AP , GANP, MAP80, SAC3, minichromosome maintenance complex component 3 associated protein, PNRIID
External IDs	OMIM: 603294; MGI: 1930089; HomoloGene: 2902; GeneCards: MCM3AP; OMA:MCM3AP - orthologs
Gene location (Human) Chr. Chromosome 21 (human) [1] Band 21q22.3 Start 46,235,133 bp [1] End 46,286,297 bp [1]
Gene location (Mouse) Chr. Chromosome 10 (mouse) [2] Band 10|10 C1 Start 76,304,761 bp [2] End 76,351,691 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in tendon of biceps brachii tibial nerve right uterine tube gastrocnemius muscle body of uterus gastric mucosa right ovary right lobe of thyroid gland cerebellar hemisphere right hemisphere of cerebellum Top expressed in saccule otic vesicle zygote primary oocyte otic placode secondary oocyte Rostral migratory stream granulocyte renal corpuscle ventricular zone More reference expression data BioGPS More reference expression data
Gene ontology Molecular function transferase activity DNA binding acyltransferase activity nucleic acid binding chromatin binding histone acetyltransferase activity protein binding H3 histone acetyltransferase activity histone binding Cellular component cytosol nuclear pore nuclear membrane nuclear envelope nucleus nucleoplasm cytoplasm transcription export complex 2 nuclear pore nuclear basket chromosome Biological process protein transport mRNA transport protein import into nucleus immune system process transport mRNA export from nucleus poly(A)+ mRNA export from nucleus somatic hypermutation of immunoglobulin genes histone acetylation nucleosome organization histone H3 acetylation Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 8888 54387 Ensembl ENSG00000160294 ENSMUSG00000001150 UniProt O60318 Q9WUU9 RefSeq (mRNA) NM_003906 NM_019434 RefSeq (protein) NP_003897 NP_062307 Location (UCSC) Chr 21: 46.24 – 46.29 Mb Chr 10: 76.3 – 76.35 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

80 kDa MCM3-associated protein is a protein that in humans is encoded by the MCM3AP gene. ^{[5] [6]}

Function

The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. The protein encoded by this gene is an MCM3 binding protein. It was reported to have phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. This protein was demonstrated to be an acetyltransferase that acetylates MCM3 and plays a role in DNA replication. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that this protein may also facilitate MCM3 nuclear localization. ^[6]

Interactions

MCM3AP has been shown to interact with:

• MCM3 ^{[5] [7] [8]} and
• TRIB3. ^[9]

References

1. GRCh38: Ensembl release 89: ENSG00000160294 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000001150 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Takei Y, Tsujimoto G (September 1998). "Identification of a novel MCM3-associated protein that facilitates MCM3 nuclear localization". J. Biol. Chem. 273 (35): 22177–80. doi: 10.1074/jbc.273.35.22177 . PMID   9712829.
6. "Entrez Gene: MCM3AP MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) associated protein".
7. Takei Y, Assenberg M, Tsujimoto G, Laskey R (November 2002). "The MCM3 acetylase MCM3AP inhibits initiation, but not elongation, of DNA replication via interaction with MCM3". J. Biol. Chem. 277 (45): 43121–5. doi: 10.1074/jbc.C200442200 . PMID   12226073.
8. Kuwahara K, Yoshida M, Kondo E, Sakata A, Watanabe Y, Abe E, Kouno Y, Tomiyasu S, Fujimura S, Tokuhisa T, Kimura H, Ezaki T, Sakaguchi N (April 2000). "A novel nuclear phosphoprotein, GANP, is up-regulated in centrocytes of the germinal center and associated with MCM3, a protein essential for DNA replication". Blood. 95 (7): 2321–8. doi:10.1182/blood.V95.7.2321. PMID   10733502.
9. Zhou Y, Li L, Liu Q, Xing G, Kuai X, Sun J, Yin X, Wang J, Zhang L, He F (May 2008). "E3 ubiquitin ligase SIAH1 mediates ubiquitination and degradation of TRB3". Cell. Signal. 20 (5): 942–8. doi:10.1016/j.cellsig.2008.01.010. PMID   18276110.

Further reading

• Nagase T, Ishikawa K, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (1998). "Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Res. 5 (1): 31–9. doi: 10.1093/dnares/5.1.31 . PMID   9628581.
• Kuwahara K, Yoshida M, Kondo E, Sakata A, Watanabe Y, Abe E, Kouno Y, Tomiyasu S, Fujimura S, Tokuhisa T, Kimura H, Ezaki T, Sakaguchi N (2000). "A novel nuclear phosphoprotein, GANP, is up-regulated in centrocytes of the germinal center and associated with MCM3, a protein essential for DNA replication". Blood. 95 (7): 2321–8. doi:10.1182/blood.V95.7.2321. PMID   10733502.
• Abe E, Kuwahara K, Yoshida M, Suzuki M, Terasaki H, Matsuo Y, Takahashi EI, Sakaguchi N (2000). "Structure, expression, and chromosomal localization of the human gene encoding a germinal center-associated nuclear protein (GANP) that associates with MCM3 involved in the initiation of DNA replication". Gene. 255 (2): 219–27. doi:10.1016/S0378-1119(00)00336-X. PMID   11024281.
• Takei Y, Swietlik M, Tanoue A, Tsujimoto G, Kouzarides T, Laskey R (2001). "MCM3AP, a novel acetyltransferase that acetylates replication protein MCM3". EMBO Rep. 2 (2): 119–23. doi:10.1093/embo-reports/kve026. PMC   1083822 . PMID   11258703.
• Kuwahara K, Tomiyasu S, Fujimura S, Nomura K, Xing Y, Nishiyama N, Ogawa M, Imajoh-Ohmi S, Izuta S, Sakaguchi N (2001). "Germinal center-associated nuclear protein (GANP) has a phosphorylation-dependent DNA-primase activity that is up-regulated in germinal center regions". Proc. Natl. Acad. Sci. U.S.A. 98 (18): 10279–83. Bibcode:2001PNAS...9810279K. doi: 10.1073/pnas.181335698 . PMC   56952 . PMID   11526238.
• EL-Gazzar MA, Maeda K, Nomiyama H, Nakao M, Kuwahara K, Sakaguchi N (2001). "PU.1 is involved in the regulation of B lineage-associated and developmental stage-dependent expression of the germinal center-associated DNA primase GANP". J. Biol. Chem. 276 (51): 48000–8. doi: 10.1074/jbc.M106696200 . PMID   11641399.
• Kono Y, Maeda K, Kuwahara K, Yamamoto H, Miyamoto E, Yonezawa K, Takagi K, Sakaguchi N (2002). "MCM3-binding GANP DNA-primase is associated with a novel phosphatase component G5PR". Genes Cells. 7 (8): 821–34. doi: 10.1046/j.1365-2443.2002.00562.x . PMID   12167160. S2CID   21946802.
• Takei Y, Assenberg M, Tsujimoto G, Laskey R (2002). "The MCM3 acetylase MCM3AP inhibits initiation, but not elongation, of DNA replication via interaction with MCM3". J. Biol. Chem. 277 (45): 43121–5. doi: 10.1074/jbc.C200442200 . PMID   12226073.
• Nakayama M, Kikuno R, Ohara O (2002). "Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs". Genome Res. 12 (11): 1773–84. doi:10.1101/gr.406902. PMC   187542 . PMID   12421765.
• Sakaguchi N, Fujimura S, Kuwahara K (2002). "Involvement of GANP in B cell activation in T cell-dependent antigen response". Dev. Immunol. 9 (3): 169–72. doi:10.1080/1044667031000137647. PMC   2276099 . PMID   12885157.
• Colland F, Jacq X, Trouplin V, Mougin C, Groizeleau C, Hamburger A, Meil A, Wojcik J, Legrain P, Gauthier JM (2004). "Functional proteomics mapping of a human signaling pathway". Genome Res. 14 (7): 1324–32. doi:10.1101/gr.2334104. PMC   442148 . PMID   15231748.
• Hu YH, Warnatz HJ, Vanhecke D, Wagner F, Fiebitz A, Thamm S, Kahlem P, Lehrach H, Yaspo ML, Janitz M (2006). "Cell array-based intracellular localization screening reveals novel functional features of human chromosome 21 proteins". BMC Genomics. 7: 155. doi: 10.1186/1471-2164-7-155 . PMC   1526728 . PMID   16780588.

External links

• PDBe-KB provides an overview of all the structure information available in the PDB for Human Germinal-center associated nuclear protein (MCM3AP)