MED28

MED28
Identifiers
Aliases	MED28 , 1500003D12Rik, EG1, magicin, mediator complex subunit 28
External IDs	OMIM: 610311; MGI: 1914249; HomoloGene: 11873; GeneCards: MED28; OMA:MED28 - orthologs
Gene location (Human) Chr. Chromosome 4 (human) [1] Band 4p15.32 Start 17,614,641 bp [1] End 17,634,105 bp [1]
Gene location (Mouse) Chr. Chromosome 5 (mouse) [2] Band 5|5 B3 Start 45,677,571 bp [2] End 45,686,618 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in amniotic fluid buccal mucosa cell ventricular zone monocyte palpebral conjunctiva embryo ganglionic eminence thymus gastrocnemius muscle cartilage tissue Top expressed in primary oocyte medial ganglionic eminence spermatid right kidney zygote proximal tubule spermatocyte endocardial cushion seminiferous tubule arcuate nucleus More reference expression data BioGPS More reference expression data
Gene ontology Molecular function actin binding protein binding Cellular component cytoplasm mediator complex cytoskeleton membrane nucleus cortical actin cytoskeleton nucleoplasm Biological process stem cell population maintenance regulation of transcription, DNA-templated negative regulation of smooth muscle cell differentiation transcription, DNA-templated Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 80306 66999 Ensembl ENSG00000118579 ENSMUSG00000015804 UniProt Q9H204 Q920D3 RefSeq (mRNA) NM_025205 NM_025895 RefSeq (protein) NP_079481 NP_080171 Location (UCSC) Chr 4: 17.61 – 17.63 Mb Chr 5: 45.68 – 45.69 Mb PubMed search [3] [4]
Wikidata
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Med28
Identifiers
Symbol	Med28
Pfam	PF11594
InterPro	IPR021640
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

Mediator of RNA polymerase II transcription subunit 28 is an enzyme that in humans is encoded by the MED28 gene. ^{[5] [6] [7]} It forms part of the Mediator complex.

Function

Subunit Med28 of the Mediator may function as a scaffolding protein within Mediator by maintaining the stability of a submodule within the head module, and components of this submodule act together in a gene-regulatory programme to suppress smooth muscle cell differentiation. Thus, mammalian Mediator subunit Med28 functions as a repressor of smooth muscle-cell differentiation, which could have implications for disorders associated with abnormalities in smooth muscle cell growth and differentiation, including atherosclerosis, asthma, hypertension, and smooth muscle tumours. ^[8]

Interactions

MED28 has been shown to interact with Merlin, ^[6] Grb2 ^[6] and MED26. ^[9]

See also

• Mediator complex

References

1. GRCh38: Ensembl release 89: ENSG00000118579 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000015804 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Liu C, Zhang L, Shao ZM, Beatty P, Sartippour M, Lane TF, Barsky SH, Livingston E, Nguyen M (Jan 2002). "Identification of a novel endothelial-derived gene EG-1". Biochemical and Biophysical Research Communications. 290 (1): 602–12. doi:10.1006/bbrc.2001.6119. PMID   11779215.
6. Wiederhold T, Lee MF, James M, Neujahr R, Smith N, Murthy A, Hartwig J, Gusella JF, Ramesh V (Nov 2004). "Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2". Oncogene. 23 (54): 8815–25. doi: 10.1038/sj.onc.1208110 . PMID   15467741.
7. "Entrez Gene: MED28 mediator of RNA polymerase II transcription, subunit 28 homolog (S. cerevisiae)".
8. Beyer KS, Beauchamp RL, Lee MF, Gusella JF, Näär AM, Ramesh V (Nov 2007). "Mediator subunit MED28 (Magicin) is a repressor of smooth muscle cell differentiation". The Journal of Biological Chemistry. 282 (44): 32152–7. doi: 10.1074/jbc.M706592200 . PMID   17848560.
9. Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC (Jun 2004). "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology". Molecular Cell. 14 (5): 685–91. doi: 10.1016/j.molcel.2004.05.006 . PMID   15175163.

This article incorporates text from the public domain Pfam and InterPro: IPR021640

Further reading

• Suzuki H, Fukunishi Y, Kagawa I, Saito R, Oda H, Endo T, Kondo S, Bono H, Okazaki Y, Hayashizaki Y (Oct 2001). "Protein-protein interaction panel using mouse full-length cDNAs". Genome Research. 11 (10): 1758–65. doi:10.1101/gr.180101. PMC   311163 . PMID   11591653.
• Sato S, Tomomori-Sato C, Banks CA, Sorokina I, Parmely TJ, Kong SE, Jin J, Cai Y, Lane WS, Brower CS, Conaway RC, Conaway JW (Apr 2003). "Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3". The Journal of Biological Chemistry. 278 (17): 15123–7. doi: 10.1074/jbc.C300054200 . PMID   12584197.
• Tomomori-Sato C, Sato S, Parmely TJ, Banks CA, Sorokina I, Florens L, Zybailov B, Washburn MP, Brower CS, Conaway RC, Conaway JW (Feb 2004). "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2". The Journal of Biological Chemistry. 279 (7): 5846–51. doi: 10.1074/jbc.M312523200 . PMID   14638676.
• Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC (Jun 2004). "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology". Molecular Cell. 14 (5): 685–91. doi: 10.1016/j.molcel.2004.05.006 . PMID   15175163.
• Lu M, Zhang L, Maul RS, Sartippour MR, Norris A, Whitelegge J, Rao JY, Brooks MN (Jul 2005). "The novel gene EG-1 stimulates cellular proliferation". Cancer Research. 65 (14): 6159–66. doi:10.1158/0008-5472.CAN-04-4016. PMID   16024617.
• Lee MF, Beauchamp RL, Beyer KS, Gusella JF, Ramesh V (Sep 2006). "Magicin associates with the Src-family kinases and is phosphorylated upon CD3 stimulation". Biochemical and Biophysical Research Communications. 348 (3): 826–31. doi:10.1016/j.bbrc.2006.07.126. PMID   16899217.
• Lu M, Zhang L, Sartippour MR, Norris AJ, Brooks MN (Oct 2006). "EG-1 interacts with c-Src and activates its signaling pathway". International Journal of Oncology. 29 (4): 1013–8. doi: 10.3892/ijo.29.4.1013 . PMID   16964398.
• Beyer KS, Beauchamp RL, Lee MF, Gusella JF, Näär AM, Ramesh V (Nov 2007). "Mediator subunit MED28 (Magicin) is a repressor of smooth muscle cell differentiation". The Journal of Biological Chemistry. 282 (44): 32152–7. doi: 10.1074/jbc.M706592200 . PMID   17848560.