Melike Lakadamyali

Last updated
Melike Lakadamyali
NationalityCypriot
Alma mater
Known for Super-Resolution Microscopy
Single Molecule Biophysics
Scientific career
Fields Super-Resolution Microscopy
Single Molecule Biophysics
Institutions
Thesis Real-time Imaging of Viral Infection and Intracellular Transport in Live Cells  (2006)
Academic advisors Xiaowei Zhuang
Jeff Lichtman
Website Lakadamyali Lab

Melike Lakadamyali is a Cypriot physicist and an Associate Professor of Physiology and of Cell and Developmental Biology (secondary) at the University of Pennsylvania in Philadelphia, renowned for her work in super-resolution microscopy and Single Molecule Biophysics. She is the Group Leader of the Lakadamyali Lab. [1]

Contents

Education

From 1997 to 2001, Lakadamyali studied Physics at the University of Texas at Austin, USA. During the end of her university time, she gained some practical experience by working in the labs of Prof. Ken Shih and Prof. Josef A. Käs. From 2001 to 2006, she earned her Ph.D. in Physics at Harvard University, Cambridge, MA, USA, advised by Prof Xiaowei Zhuang, [2] [3] [4] focusing on the visualization of viral infection and intracellular transport in live cells. [5] [6] [7]

Career and research

For her postgraduate work, Lakadamyali worked as a postdoctoral researcher under Prof Jeff Lichtman at the Center for Brain Science at Harvard University, MA, USA. [8]

Between 2010 and 2016, Lakadamyali was a Group Leader at ICFO - The Institute of Photonic Sciences in Barcelona, Spain, holding a Junior (2010-2015) and Senior (2015-2016) Group Leader position, respectively. Her group's superresolution microscopy study investigating the genome gained widespread attention. It reveals that our genome needs to be regularly packaged and links these packaging differences to stem cell state. [9] [10] [11]

In 2017, Lakadamyali returned to the United States to work as an Assistant Professor of Physiology and of Cell and Developmental Biology (secondary) at the University of Pennsylvania in Philadelphia.

In 2020, Lakadamyali was promoted to Associate Professor of Physiology and in 2024 she was promoted to Full Professor.

Lakadamyali's area of research is focused on examining biology at the level of its macromolecular machines. She aims to obtain a quantitative and biophysical comprehension of how these machines propel critical cell biological processes. [12] [13] [14] Hence, she is also involved in designing sophisticated microscopy techniques that strive to surmount the current limitations of existing methods, thereby enabling them to observe the macromolecular machinery of the cell in motion with superior spatiotemporal resolution. [15] [16] [17]

Lakadamyali is a well-known microscopist and biophysicist. Hence, she is frequently invited to speak at workshops [18] [19] and conferences [20] [21] [22] in the field.

Since 2019, Lakadamyali has been a Reviewing Editor (Cell Biology) at eLife. [23]

Awards and honours

Related Research Articles

<span class="mw-page-title-main">Nucleosome</span> Basic structural unit of DNA packaging in eukaryotes

A nucleosome is the basic structural unit of DNA packaging in eukaryotes. The structure of a nucleosome consists of a segment of DNA wound around eight histone proteins and resembles thread wrapped around a spool. The nucleosome is the fundamental subunit of chromatin. Each nucleosome is composed of a little less than two turns of DNA wrapped around a set of eight proteins called histones, which are known as a histone octamer. Each histone octamer is composed of two copies each of the histone proteins H2A, H2B, H3, and H4.

ChIP-sequencing, also known as ChIP-seq, is a method used to analyze protein interactions with DNA. ChIP-seq combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. It can be used to map global binding sites precisely for any protein of interest. Previously, ChIP-on-chip was the most common technique utilized to study these protein–DNA relations.

Super-resolution microscopy is a series of techniques in optical microscopy that allow such images to have resolutions higher than those imposed by the diffraction limit, which is due to the diffraction of light. Super-resolution imaging techniques rely on the near-field or on the far-field. Among techniques that rely on the latter are those that improve the resolution only modestly beyond the diffraction-limit, such as confocal microscopy with closed pinhole or aided by computational methods such as deconvolution or detector-based pixel reassignment, the 4Pi microscope, and structured-illumination microscopy technologies such as SIM and SMI.

Xiaowei Zhuang is a Chinese-American biophysicist who is the David B. Arnold Jr. Professor of Science, Professor of Chemistry and Chemical Biology, and Professor of Physics at Harvard University, and an Investigator at the Howard Hughes Medical Institute. She is best known for her work in the development of Stochastic Optical Reconstruction Microscopy (STORM), a super-resolution fluorescence microscopy method, and the discoveries of novel cellular structures using STORM. She received a 2019 Breakthrough Prize in Life Sciences for developing super-resolution imaging techniques that get past the diffraction limits of traditional light microscopes, allowing scientists to visualize small structures within living cells. She was elected a Member of the American Philosophical Society in 2019 and was awarded a Vilcek Foundation Prize in Biomedical Science in 2020.

<span class="mw-page-title-main">David L. Spector</span>

David L. Spector is a cell and molecular biologist best recognized for his research on gene expression and nuclear dynamics. He is currently a Professor at Cold Spring Harbor Laboratory (CSHL). From 2007 to 2023, he served as Director of Research of CSHL.

Sir David Klenerman is a British biophysical chemist and a professor of biophysical chemistry at the Department of Chemistry at the University of Cambridge and a Fellow of Christ's College, Cambridge.

H3K4me3 is an epigenetic modification to the DNA packaging protein Histone H3 that indicates tri-methylation at the 4th lysine residue of the histone H3 protein and is often involved in the regulation of gene expression. The name denotes the addition of three methyl groups (trimethylation) to the lysine 4 on the histone H3 protein.

H3K9me3 is an epigenetic modification to the DNA packaging protein Histone H3. It is a mark that indicates the tri-methylation at the 9th lysine residue of the histone H3 protein and is often associated with heterochromatin.

H3K4me1 is an epigenetic modification to the DNA packaging protein Histone H3. It is a mark that indicates the mono-methylation at the 4th lysine residue of the histone H3 protein and often associated with gene enhancers.

H3K36me3 is an epigenetic modification to the DNA packaging protein Histone H3. It is a mark that indicates the tri-methylation at the 36th lysine residue of the histone H3 protein and often associated with gene bodies.

H4K20me is an epigenetic modification to the DNA packaging protein Histone H4. It is a mark that indicates the mono-methylation at the 20th lysine residue of the histone H4 protein. This mark can be di- and tri-methylated. It is critical for genome integrity including DNA damage repair, DNA replication and chromatin compaction.

H4K12ac is an epigenetic modification to the DNA packaging protein histone H4. It is a mark that indicates the acetylation at the 12th lysine residue of the histone H4 protein. H4K12ac is involved in learning and memory. It is possible that restoring this modification could reduce age-related decline in memory.

H3K9ac is an epigenetic modification to the DNA packaging protein Histone H3. It is a mark that indicates the acetylation at the 9th lysine residue of the histone H3 protein.

H3K36me2 is an epigenetic modification to the DNA packaging protein Histone H3. It is a mark that indicates the di-methylation at the 36th lysine residue of the histone H3 protein.

H3K36me is an epigenetic modification to the DNA packaging protein Histone H3, specifically, the mono-methylation at the 36th lysine residue of the histone H3 protein.

<span class="mw-page-title-main">Beat Fierz</span> Swiss chemist and molecular biologist

Beat Fierz is a Swiss chemist and molecular biologist, currently an Associate Professor at the EPFL. His research is focused on understanding dynamic processes of large molecular systems, particularly in chromatin regulation.

Timothy John Richmond is a Swiss/American molecular biologist, biochemist, and biophysicist.

<span class="mw-page-title-main">Nicolas H. Thomä</span> German structural and chemical biologist

Nicolas H. Thomä is a German researcher, full professor at the EPFL School of Life Sciences and Director of the Paternot Chair for Cancer Research in Lausanne, Switzerland. He is a biochemist and structural biologist and a leading researcher in the fields of ubiquitin ligase biology and DNA repair.

Francisco Balzarotti is an Argentinian scientist known for his work in super-resolution microscopy, particularly MINFLUX. He is a Group Leader at the Research Institute of Molecular Pathology (IMP) in Vienna, Austria.

Gaia Pigino is the Associate Head of the Structural Biology Research Center and Research Group Leader of the Pigino Group at the Human Technopole in Milan, Italy.

References

  1. ↑ "Lakadamyali Lab". Lakadamyali Lab. Retrieved 2023-09-04.
  2. ↑ "Curriculum Vitae: Melike Lakadamyali" (PDF). static1.squarespace.com. Retrieved 4 September 2023.
  3. ↑ "Melike Lakadamyali". AcademiaNet. Retrieved 4 September 2023.
  4. 1 2 "Cell scientist to watch − Melike Lakadamyali | Journal of Cell Science | The Company of Biologists". Journals.biologists.com. 2017-05-15. Retrieved 2023-09-04.
  5. ↑ Lakadamyali, Melike; Rust, Michael J.; Babcock, Hazen P.; Zhuang, Xiaowei (2003-08-05). "Visualizing infection of individual influenza viruses". Proceedings of the National Academy of Sciences. 100 (16): 9280–9285. Bibcode:2003PNAS..100.9280L. doi: 10.1073/pnas.0832269100 . ISSN   0027-8424. PMC   170909 . PMID   12883000.
  6. ↑ Rust, M. J.; Lakadamyali, M.; Brandenburg, B.; Zhuang, X. (2011-09-01). "Single-Particle Virus Tracking". Cold Spring Harbor Protocols. 2011 (9). Cshprotocols.cshlp.org: pdb.prot065631. doi:10.1101/pdb.prot065631. PMID   21880810 . Retrieved 2023-09-04.
  7. ↑ Rust, M. J.; Lakadamyali, M.; Brandenburg, B.; Zhuang, X. (2011-09-01). "Single-Virus Tracking in Live Cells". Cold Spring Harbor Protocols. 2011 (9). Cshprotocols.cshlp.org: pdb.top065623. doi:10.1101/pdb.top065623. PMID   21880811 . Retrieved 2023-09-04.
  8. ↑ Lakadamyali, M.; Babcock, H.; Bates, M.; Zhuang, X.; Lichtman, J. (2012-01-24). "3D Multicolor Super-Resolution Imaging Offers Improved Accuracy in Neuron Tracing | PLOS ONE". PLOS ONE. 7 (1). Journals.plos.org: e30826. doi: 10.1371/journal.pone.0030826 . PMC   3265519 . PMID   22292051.
  9. ↑ Ricci, Maria Aurelia; Manzo, Carlo; García-Parajo, María Filomena; Lakadamyali, Melike; Pia Cosma, Maria (12 March 2015). "Chromatin Fibers Are Formed by Heterogeneous Groups of Nucleosomes In Vivo". Cell . 160 (6): 1145–1158. doi: 10.1016/j.cell.2015.01.054 . hdl: 10803/298724 . PMID   25768910.
  10. ↑ "Nucleosome clutches / Cell, March 12, 2015 (Vol. 160, Issue 6)". YouTube. Retrieved 2023-09-04.
  11. ↑ "Centre for Genomic Regulation Website". Crg.eu. 2015-03-12. Retrieved 2023-09-04.
  12. ↑ "Super resolution microscopy reveals how elongating RNA polymerase II and nascent RNA interact with nucleosome clutches | Nucleic Acids Research | Oxford Academic". Academic.oup.com. Retrieved 2023-09-04.
  13. ↑ Gómez-García, Pablo Aurelio; Portillo-Ledesma, Stephanie; Neguembor, Maria Victoria; Pesaresi, Martina; Oweis, Walaa; Rohrlich, Talia; Wieser, Stefan; Meshorer, Eran; Schlick, Tamar; Cosma, Maria Pia; Lakadamyali, Melike (2021). "Mesoscale Modeling and Single-Nucleosome Tracking Reveal Remodeling of Clutch Folding and Dynamics in Stem Cell Differentiation". Cell Reports. 34 (2). doi:10.1016/j.celrep.2020.108614. PMC   7842188 . PMID   33440158.
  14. ↑ Lakadamyali, Melike; Cosma, Maria Pia (2020). "Visualizing the genome in high resolution challenges our textbook understanding". Nature Methods. 17 (4): 371–379. doi:10.1038/s41592-020-0758-3. hdl: 10230/43808 . ISSN   1548-7105. PMID   32123395. S2CID   211729851.
  15. ↑ "Melike Lakadamyali | Faculty | About Us | Perelman School of Medicine | Perelman School of Medicine at the University of Pennsylvania". Med.upenn.edu. Retrieved 2023-09-04.
  16. ↑ Bond, Charles; Santiago-Ruiz, Adriana N.; Tang, Qing; Lakadamyali, Melike (2022-01-20). "Technological advances in super-resolution microscopy to study cellular processes". Molecular Cell. 82 (2): 315–332. doi:10.1016/j.molcel.2021.12.022. PMC   8852216 . PMID   35063099.
  17. ↑ Hugelier, Siewert; Colosi, P.L.; Lakadamyali, Melike (2023-05-09). "Quantitative Single-Molecule Localization Microscopy". Annual Review of Biophysics. 52 (1): 139–160. doi: 10.1146/annurev-biophys-111622-091212 . ISSN   1936-122X. PMID   37159293.
  18. ↑ "In situ structural biology: from cryo-EM to multi-scale modelling – Course and Conference Office". Embl.org. Retrieved 2023-09-04.
  19. ↑ Home. "22nd Single Molecule Workshop". PicoQuant. Retrieved 2023-09-04.
  20. ↑ "JCS2023: Imaging Cell Dynamics - The Company of Biologists". Biologists.com. Retrieved 2023-09-04.
  21. ↑ "Seeing is Believing – Imaging the Molecular Processes of Life – Course and Conference Office". Embl.org. 2019-10-12. Retrieved 2023-09-04.
  22. ↑ "Genome Organization & Nuclear Function 2022 | CSHL". meetings.cshl.edu. Retrieved 2023-09-04.
  23. ↑ "Editors for Cell Biology". eLife. Retrieved 2023-09-04.
  24. ↑ "EMBO Young Investigator Program". ICFO. Retrieved 2023-09-04.
  25. ↑ "Physical Sciences and Engineering - List of Principal Investigators" (PDF). erc.europa.eu. Retrieved 4 September 2023.
  26. ↑ "On the move: Motor-cargo and motor-microtubule interactions studied with quantitative, high spatio-temporal resolution microscopy in vivo". CORDIS. Retrieved 4 September 2023.
  27. ↑ "ERC Starting Grant Awarded to Prof. Melike Lakadamyali". ICFO. Retrieved 2023-09-04.
  28. ↑ Marx, Vivien (2017-08-01). "Melike Lakadamyali". Nature Methods. 14 (8): 755. doi: 10.1038/nmeth.4369 . ISSN   1548-7105.
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