NEK6

NEK6
Identifiers
Aliases	NEK6 , SID6-1512, NIMA related kinase 6
External IDs	OMIM: 604884 MGI: 1891638 HomoloGene: 49379 GeneCards: NEK6
Gene location (Human) Chr. Chromosome 9 (human) [1] Band 9q33.3 Start 124,257,606 bp [1] End 124,353,307 bp [1]
Gene location (Mouse) Chr. Chromosome 2 (mouse) [2] Band 2 B|2 24.41 cM Start 38,401,655 bp [2] End 38,484,618 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in sural nerve stromal cell of endometrium right lobe of liver appendix gallbladder pancreatic ductal cell monocyte bone marrow cells rectum caudate nucleus Top expressed in jejunum neural tube placenta yolk sac molar intestinal villus lens Paneth cell proximal tubule hepatobiliary system More reference expression data BioGPS n/a
Gene ontology Molecular function transferase activity nucleotide binding protein kinase activity signal transducer activity metal ion binding kinase activity protein binding kinesin binding ATP binding protein kinase binding magnesium ion binding protein serine/threonine kinase activity transcription corepressor binding ubiquitin protein ligase binding Cellular component cytoplasm nuclear speck spindle pole intracellular membrane-bounded organelle nucleoplasm microtubule organizing center microtubule cytoskeleton nucleus cytosol centrosome Biological process phosphorylation chromosome segregation spindle assembly cell division protein phosphorylation mitotic nuclear membrane disassembly peptidyl-serine phosphorylation regulation of mitotic cell cycle regulation of mitotic metaphase/anaphase transition positive regulation of I-kappaB kinase/NF-kappaB signaling protein autophosphorylation regulation of cellular senescence cell cycle apoptotic process signal transduction Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 10783 59126 Ensembl ENSG00000119408 ENSMUSG00000026749 UniProt Q9HC98 Q5TBG4 Q9ES70 RefSeq (mRNA) NM_001145001 NM_001166167 NM_001166168 NM_001166169 NM_001166170 NM_001166171 NM_014397 NM_001159631 NM_021606 RefSeq (protein) NP_001138473 NP_001159639 NP_001159640 NP_001159641 NP_001159642 NP_001159643 NP_055212 NP_001153103 NP_067619 Location (UCSC) Chr 9: 124.26 – 124.35 Mb Chr 2: 38.4 – 38.48 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Serine/threonine-protein kinase Nek6 is an enzyme that in humans is encoded by the NEK6 gene. ^{[5] [6]}

Function

The Aspergillus nidulans 'never in mitosis A' (NIMA) gene encodes a serine/threonine kinase that controls initiation of mitosis. NIMA-related kinases (NEKs) are a group of protein kinases that are homologous to NIMA. Evidence suggests that NEKs perform functions similar to those of NIMA. ^[6]

It is a protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4, STAT3 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence. ^[7]

Interactions

NEK6 has been shown to interact with NEK9. ^{[8] [9] [10]}

References

1. GRCh38: Ensembl release 89: ENSG00000119408 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000026749 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Li MZ, Yu L, Liu Q, Chu JY, Zhao SY (April 2000). "Assignment of NEK6, a NIMA-related gene, to human chromosome 9q33. 3→q34.11 by radiation hybrid mapping". Cytogenet Cell Genet. 87 (3–4): 271–2. doi:10.1159/000015445. PMID   10702691. S2CID   46463586.
6. "Entrez Gene: NEK6 NIMA (never in mitosis gene a)-related kinase 6".
7. Q9HC98
8. Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC   1847948 . PMID   17353931.
9. Belham C, Roig J, Caldwell JA, Aoyama Y, Kemp BE, Comb M, Avruch J (September 2003). "A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases". J. Biol. Chem. 278 (37): 34897–909. doi: 10.1074/jbc.M303663200 . PMID   12840024.
10. Roig J, Mikhailov A, Belham C, Avruch J (July 2002). "Nercc1, a mammalian NIMA-family kinase, binds the Ran GTPase and regulates mitotic progression". Genes Dev. 16 (13): 1640–58. doi:10.1101/gad.972202. PMC   186374 . PMID   12101123.

Further reading

• Kobayashi T, Cohen P (1999). "Activation of serum- and glucocorticoid-regulated protein kinase by agonists that activate phosphatidylinositide 3-kinase is mediated by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and PDK2". Biochem. J. 339 ( Pt 2) (2): 319–28. doi:10.1042/0264-6021:3390319. PMC   1220160 . PMID   10191262.
• Kandli M, Feige E, Chen A, Kilfin G, Motro B (2001). "Isolation and characterization of two evolutionarily conserved murine kinases (Nek6 and nek7) related to the fungal mitotic regulator, NIMA". Genomics. 68 (2): 187–96. doi:10.1006/geno.2000.6293. PMID   10964517.
• Belham C, Comb MJ, Avruch J (2001). "Identification of the NIMA family kinases NEK6/7 as regulators of the p70 ribosomal S6 kinase". Curr. Biol. 11 (15): 1155–67. Bibcode:2001CBio...11.1155B. doi: 10.1016/S0960-9822(01)00369-4 . PMID   11516946. S2CID   15509230.
• Kimura M, Okano Y (2001). "Identification and assignment of the human NIMA-related protein kinase 7 gene (NEK7) to human chromosome 1q31.3". Cytogenet. Cell Genet. 94 (1–2): 33–8. doi:10.1159/000048779. PMID   11701951. S2CID   27666742.
• Lizcano JM, Deak M, Morrice N, Kieloch A, Hastie CJ, Dong L, Schutkowski M, Reimer U, Alessi DR (2002). "Molecular basis for the substrate specificity of NIMA-related kinase-6 (NEK6). Evidence that NEK6 does not phosphorylate the hydrophobic motif of ribosomal S6 protein kinase and serum- and glucocorticoid-induced protein kinase in vivo". J. Biol. Chem. 277 (31): 27839–49. doi: 10.1074/jbc.M202042200 . PMID   12023960.
• Hashimoto Y, Akita H, Hibino M, Kohri K, Nakanishi M (2002). "Identification and characterization of Nek6 protein kinase, a potential human homolog of NIMA histone H3 kinase". Biochem. Biophys. Res. Commun. 293 (2): 753–8. doi:10.1016/S0006-291X(02)00297-8. PMID   12054534.
• Roig J, Mikhailov A, Belham C, Avruch J (2002). "Nercc1, a mammalian NIMA-family kinase, binds the Ran GTPase and regulates mitotic progression". Genes Dev. 16 (13): 1640–58. doi:10.1101/gad.972202. PMC   186374 . PMID   12101123.
• Matsuda A, Suzuki Y, Honda G, Muramatsu S, Matsuzaki O, Nagano Y, Doi T, Shimotohno K, Harada T, Nishida E, Hayashi H, Sugano S (2003). "Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways". Oncogene. 22 (21): 3307–18. doi:10.1038/sj.onc.1206406. PMID   12761501. S2CID   38880905.
• Belham C, Roig J, Caldwell JA, Aoyama Y, Kemp BE, Comb M, Avruch J (2003). "A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases". J. Biol. Chem. 278 (37): 34897–909. doi: 10.1074/jbc.M303663200 . PMID   12840024.
• Yin MJ, Shao L, Voehringer D, Smeal T, Jallal B (2004). "The serine/threonine kinase Nek6 is required for cell cycle progression through mitosis". J. Biol. Chem. 278 (52): 52454–60. doi: 10.1074/jbc.M308080200 . PMID   14563848.
• Barrios-Rodiles M, Brown KR, Ozdamar B, Bose R, Liu Z, Donovan RS, Shinjo F, Liu Y, Dembowy J, Taylor IW, Luga V, Przulj N, Robinson M, Suzuki H, Hayashizaki Y, Jurisica I, Wrana JL (2005). "High-throughput mapping of a dynamic signaling network in mammalian cells". Science. 307 (5715): 1621–5. Bibcode:2005Sci...307.1621B. doi:10.1126/science.1105776. PMID   15761153. S2CID   39457788.
• Chen J, Li L, Zhang Y, Yang H, Wei Y, Zhang L, Liu X, Yu L (2006). "Interaction of Pin1 with Nek6 and characterization of their expression correlation in Chinese hepatocellular carcinoma patients". Biochem. Biophys. Res. Commun. 341 (4): 1059–65. doi:10.1016/j.bbrc.2005.12.228. PMID   16476580.
• Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC   1847948 . PMID   17353931.
• Lee EJ, Hyun SH, Chun J, Kang SS (2007). "Human NIMA-related kinase 6 is one of the Fe65 WW domain binding proteins". Biochem. Biophys. Res. Commun. 358 (3): 783–8. doi:10.1016/j.bbrc.2007.04.203. PMID   17512906.