NUTF2

NUTF2
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1GY5,%%s1GY6, 5BXQ, 1A2K, 1OUN
Identifiers
Aliases	NUTF2 , NTF2, PP15, NTF-2, nuclear transport factor 2
External IDs	OMIM: 605813; MGI: 3704482; HomoloGene: 110745; GeneCards: NUTF2; OMA:NUTF2 - orthologs
Gene location (Human) Chr. Chromosome 16 (human) [1] Band 16q22.1 Start 67,846,923 bp [1] End 67,872,567 bp [1]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in muscle of thigh ascending aorta popliteal artery tibial arteries left coronary artery C1 segment Descending thoracic aorta right coronary artery anterior cingulate cortex stromal cell of endometrium n/a More reference expression data BioGPS n/a
Gene ontology Molecular function protein binding structural constituent of nuclear pore identical protein binding nuclear import signal receptor activity Cellular component cytoplasm nuclear pore central transport channel nuclear outer membrane intracellular anatomical structure extracellular exosome nucleus nuclear inner membrane nuclear pore nucleoplasm cytosol membrane nuclear membrane Biological process negative regulation of vascular endothelial growth factor production protein transport positive regulation of protein import into nucleus protein export from nucleus protein import into nucleus mRNA transport protein localization to nuclear pore transport nucleocytoplasmic transport Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 10204 621832 Ensembl ENSG00000102898 n/a UniProt P61970 P61971 RefSeq (mRNA) NM_005796 NM_001322038 NM_001322039 NM_001322040 NM_001322041 XM_001473957 RefSeq (protein) NP_001308967 NP_001308968 NP_001308969 NP_001308970 NP_005787 NP_005787.1 NP_001308967.1 NP_001308968.1 NP_001308969.1 NP_001308970.1 NP_080808 NP_001344158 NP_001344159 Location (UCSC) Chr 16: 67.85 – 67.87 Mb n/a PubMed search [2] [3]
Wikidata
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Nuclear transport factor 2 is a protein that in humans is encoded by the NUTF2 gene. ^{[4] [5] [6]}

Function

The protein encoded by this gene is a cytosolic factor that facilitates protein transport into the nucleus. It interacts with the nuclear pore complex glycoprotein p62. This encoded protein acts at a relative late stage of nuclear protein import, subsequent to the initial docking of nuclear import ligand at the nuclear envelope. It is thought to be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import. ^[6]

Interactions

NUTF2 has been shown to interact with Nucleoporin 62 ^{[4] [7]} and RAN. ^{[8] [9]}

References

1. GRCh38: Ensembl release 89: ENSG00000102898 – Ensembl, May 2017
2. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
3. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. Paschal BM, Gerace L (Jun 1995). "Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62". J Cell Biol. 129 (4): 925–937. doi:10.1083/jcb.129.4.925. PMC   2120498 . PMID   7744965.
5. Grundmann U, Nerlich C, Rein T, Lottspeich F, Küpper HA (Jul 1988). "Isolation of cDNA coding for the placental protein 15 (PP15)". Nucleic Acids Res. 16 (10): 4721. doi:10.1093/nar/16.10.4721. PMC   336660 . PMID   3380696.
6. "Entrez Gene: NUTF2 nuclear transport factor 2".
7. Hu T, Guan T, Gerace L (Aug 1996). "Molecular and functional characterization of the p62 complex, an assembly of nuclear pore complex glycoproteins". J. Cell Biol. 134 (3): 589–601. doi:10.1083/jcb.134.3.589. PMC   2120945 . PMID   8707840.
8. Cushman I, Bowman BR, Sowa ME, Lichtarge O, Quiocho FA, Moore MS (Nov 2004). "Computational and biochemical identification of a nuclear pore complex binding site on the nuclear transport carrier NTF2". J. Mol. Biol. 344 (2): 303–310. doi:10.1016/j.jmb.2004.09.043. PMID   15522285.
9. Stewart M, Kent HM, McCoy AJ (Apr 1998). "Structural basis for molecular recognition between nuclear transport factor 2 (NTF2) and the GDP-bound form of the Ras-family GTPase Ran". J. Mol. Biol. 277 (3): 635–646. doi:10.1006/jmbi.1997.1602. PMID   9533885.

Further reading

• Bukrinsky MI, Haffar OK (1998). "HIV-1 nuclear import: matrix protein is back on center stage, this time together with Vpr". Mol. Med. 4 (3): 138–43. doi:10.1007/BF03401911. PMC   2230352 . PMID   9562972.
• Stewart M (2001). "Insights into the molecular mechanism of nuclear trafficking using nuclear transport factor 2 (NTF2)". Cell Struct. Funct. 25 (4): 217–225. doi: 10.1247/csf.25.217 . PMID   11129791.
• Bohn H, Johannsen R, Kraus W (1981). "[New placental protein (PP15) with immunosuppressive properties (author's transl)]". Arch. Gynecol. 230 (2): 167–72. doi:10.1007/BF02108272. PMID   7458421. S2CID   9104166.
• Hu T, Guan T, Gerace L (1996). "Molecular and functional characterization of the p62 complex, an assembly of nuclear pore complex glycoproteins". J. Cell Biol. 134 (3): 589–601. doi:10.1083/jcb.134.3.589. PMC   2120945 . PMID   8707840.
• Bullock TL, Clarkson WD, Kent HM, Stewart M (1996). "The 1.6 angstroms resolution crystal structure of nuclear transport factor 2 (NTF2)". J. Mol. Biol. 260 (3): 422–431. doi:10.1006/jmbi.1996.0411. PMID   8757804.
• Clarkson WD, Corbett AH, Paschal BM, Kent HM, McCoy AJ, Gerace L, Silver PA, Stewart M (1997). "Nuclear protein import is decreased by engineered mutants of nuclear transport factor 2 (NTF2) that do not bind GDP-Ran". J. Mol. Biol. 272 (5): 716–730. doi:10.1006/jmbi.1997.1255. PMID   9368653.
• Stewart M, Kent HM, McCoy AJ (1998). "Structural basis for molecular recognition between nuclear transport factor 2 (NTF2) and the GDP-bound form of the Ras-family GTPase Ran". J. Mol. Biol. 277 (3): 635–646. doi:10.1006/jmbi.1997.1602. PMID   9533885.
• Ribbeck K, Lipowsky G, Kent HM, Stewart M, Görlich D (1999). "NTF2 mediates nuclear import of Ran". EMBO J. 17 (22): 6587–6598. doi:10.1093/emboj/17.22.6587. PMC   1171005 . PMID   9822603.
• Steggerda SM, Black BE, Paschal BM (2000). "Monoclonal antibodies to NTF2 inhibit nuclear protein import by preventing nuclear translocation of the GTPase Ran". Mol. Biol. Cell. 11 (2): 703–19. doi:10.1091/mbc.11.2.703. PMC   14804 . PMID   10679025.
• Freire J, Covelo G, Sarandeses C, Díaz-Jullien C, Freire M (2001). "Identification of nuclear-import and cell-cycle regulatory proteins that bind to prothymosin alpha". Biochem. Cell Biol. 79 (2): 123–131. doi:10.1139/bcb-79-2-123. PMID   11310559.
• Cushman I, Bowman BR, Sowa ME, Lichtarge O, Quiocho FA, Moore MS (2004). "Computational and biochemical identification of a nuclear pore complex binding site on the nuclear transport carrier NTF2". J. Mol. Biol. 344 (2): 303–310. doi:10.1016/j.jmb.2004.09.043. PMID   15522285.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–1178. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID   16189514. S2CID   4427026.

External links

• NUTF2 human gene location in the UCSC Genome Browser .
• NUTF2 human gene details in the UCSC Genome Browser .