PTRF

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CAVIN1
Identifiers
Aliases	CAVIN1 , CAVIN, CGL4, cavin-1, FKSG13, PTRF, polymerase I and transcript release factor, caveolae associated protein 1
External IDs	OMIM: 603198; MGI: 1277968; HomoloGene: 7499; GeneCards: CAVIN1; OMA:CAVIN1 - orthologs
Gene location (Human) Chr. Chromosome 17 (human) [1] Band 17q21.2 Start 42,402,449 bp [1] End 42,423,256 bp [1]
Gene location (Mouse) Chr. Chromosome 11 (mouse) [2] Band 11 D|11 63.95 cM Start 100,847,559 bp [2] End 100,861,713 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in right coronary artery tendon of biceps brachii popliteal artery tibial arteries Descending thoracic aorta saphenous vein ascending aorta left coronary artery body of uterus gastric mucosa Top expressed in lactiferous gland ascending aorta aortic valve umbilical cord white adipose tissue ankle joint gastrula subcutaneous adipose tissue plantaris muscle tunica media of zone of aorta More reference expression data BioGPS More reference expression data
Gene ontology Molecular function rRNA binding rRNA primary transcript binding protein binding RNA binding identical protein binding Cellular component cytoplasm cytosol membrane nucleoplasm endoplasmic reticulum mitochondrion caveola membrane raft nucleus plasma membrane intracellular membrane-bounded organelle protein-containing complex Biological process termination of RNA polymerase I transcription regulation of transcription, DNA-templated transcription initiation from RNA polymerase I promoter DNA-templated transcription, termination transcription, DNA-templated rRNA transcription positive regulation of cell motility protein secretion Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 284119 19285 Ensembl ENSG00000177469 ENSMUSG00000004044 UniProt Q6NZI2 O54724 RefSeq (mRNA) NM_012232 NM_008986 NM_001359105 NM_027160 RefSeq (protein) NP_036364 NP_033012 NP_001346034 Location (UCSC) Chr 17: 42.4 – 42.42 Mb Chr 11: 100.85 – 100.86 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Polymerase I and transcript release factor, also known as Cavin1, Cavin-1 or PTRF, is a protein which in humans is encoded by the PTRF gene. ^{[5] [6]}

Function

PTRF (Cavin1) has been shown to be crucial for caveola formation and function. ^[7] Deformation caveolae promotes the translocation of cavin-1 from caveolae to the nucleus ^[8]

Termination of RNA polymerase I catalyzed transcription is a 2-step process that involves pausing of transcription elongation and release of both the pre-ribosomal RNA and Pol I from the DNA template. The pausing is mediated by TTF1 and PTRF. ^{[9] [10]}

PTRF is a soluble protein containing putative leucine zipper, nuclear localization signal, and PEST domains. ^[11]

Interactions

PTRF (Cavin1) forms trimers with Cavin2 and Cavin3 in caveola formation and has been shown to interact with other membrane associating proteins such as EHD2 and caveolins. ^[12]

PTRF has been shown to interact with ZNF148. ^[13]

References

1. GRCh38: Ensembl release 89: ENSG00000177469 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000004044 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: PTRF polymerase I and transcript release factor".
6. "CAVIN1 Symbol Report | HUGO Gene Nomenclature Committee". www.genenames.org. Retrieved 2017-05-30.
7. Hill MM, Bastiani M, Luetterforst R, Kirkham M, Kirkham A, Nixon SJ, et al. (January 2008). "PTRF-Cavin, a conserved cytoplasmic protein required for caveola formation and function". Cell. 132 (1): 113–124. doi:10.1016/j.cell.2007.11.042. PMC   2265257 . PMID   18191225.
8. Qifti A, Balaji S, Scarlata S (June 2022). "Deformation of caveolae impacts global transcription and translation processes through relocalization of cavin-1". The Journal of Biological Chemistry. 298 (6): 102005. doi: 10.1016/j.jbc.2022.102005 . PMC   9168624 . PMID   35513070.
9. Mason SW, Sander EE, Grummt I (January 1997). "Identification of a transcript release activity acting on ternary transcription complexes containing murine RNA polymerase I". The EMBO Journal. 16 (1): 163–172. doi:10.1093/emboj/16.1.163. PMC   1169623 . PMID   9009277.
10. Jansa P, Mason SW, Hoffmann-Rohrer U, Grummt I (May 1998). "Cloning and functional characterization of PTRF, a novel protein which induces dissociation of paused ternary transcription complexes". The EMBO Journal. 17 (10): 2855–2864. doi:10.1093/emboj/17.10.2855. PMC   1170626 . PMID   9582279.
11. Aboulaich N, Vainonen JP, Strålfors P, Vener AV (October 2004). "Vectorial proteomics reveal targeting, phosphorylation and specific fragmentation of polymerase I and transcript release factor (PTRF) at the surface of caveolae in human adipocytes". The Biochemical Journal. 383 (Pt 2): 237–248. doi:10.1042/BJ20040647. PMC   1134064 . PMID   15242332.
12. Kovtun O, Tillu VA, Ariotti N, Parton RG, Collins BM (April 2015). "Cavin family proteins and the assembly of caveolae". Journal of Cell Science. 128 (7): 1269–1278. doi:10.1242/jcs.167866. PMC   4379724 . PMID   25829513.
13. Hasegawa T, Takeuchi A, Miyaishi O, Xiao H, Mao J, Isobe K (April 2000). "PTRF (polymerase I and transcript-release factor) is tissue-specific and interacts with the BFCOL1 (binding factor of a type-I collagen promoter) zinc-finger transcription factor which binds to the two mouse type-I collagen gene promoters". The Biochemical Journal. 347 Pt 1 (Pt 1): 55–59. doi:10.1042/0264-6021:3470055. PMC   1220930 . PMID   10727401.

Further reading

• Jansa P, Mason SW, Hoffmann-Rohrer U, Grummt I (May 1998). "Cloning and functional characterization of PTRF, a novel protein which induces dissociation of paused ternary transcription complexes". The EMBO Journal. 17 (10): 2855–2864. doi:10.1093/emboj/17.10.2855. PMC   1170626 . PMID   9582279.
• Hasegawa T, Takeuchi A, Miyaishi O, Xiao H, Mao J, Isobe K (April 2000). "PTRF (polymerase I and transcript-release factor) is tissue-specific and interacts with the BFCOL1 (binding factor of a type-I collagen promoter) zinc-finger transcription factor which binds to the two mouse type-I collagen gene promoters". The Biochemical Journal. 347 Pt 1 (Pt 1): 55–59. doi:10.1042/0264-6021:3470055. PMC   1220930 . PMID   10727401.
• Aboulaich N, Vainonen JP, Strålfors P, Vener AV (October 2004). "Vectorial proteomics reveal targeting, phosphorylation and specific fragmentation of polymerase I and transcript release factor (PTRF) at the surface of caveolae in human adipocytes". The Biochemical Journal. 383 (Pt 2): 237–248. doi:10.1042/BJ20040647. PMC   1134064 . PMID   15242332.
• Rush J, Moritz A, Lee KA, Guo A, Goss VL, Spek EJ, et al. (January 2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nature Biotechnology. 23 (1): 94–101. doi:10.1038/nbt1046. PMID   15592455. S2CID   7200157.
• Zhang Y, Wolf-Yadlin A, Ross PL, Pappin DJ, Rush J, Lauffenburger DA, White FM (September 2005). "Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules". Molecular & Cellular Proteomics. 4 (9): 1240–1250. doi: 10.1074/mcp.M500089-MCP200 . PMID   15951569.
• Aboulaich N, Ortegren U, Vener AV, Strålfors P (November 2006). "Association and insulin regulated translocation of hormone-sensitive lipase with PTRF". Biochemical and Biophysical Research Communications. 350 (3): 657–661. doi:10.1016/j.bbrc.2006.09.094. PMID   17026959.
• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (November 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–648. doi: 10.1016/j.cell.2006.09.026 . PMID   17081983. S2CID   7827573.