Parallel-plate flow chamber

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A parallel-plate fluid flow chamber is a benchtop (in vitro) model that simulates fluid shear stresses on various cell types exposed to dynamic fluid flow in their natural, physiological environment. The metabolic response of cells in vitro is associated with the wall shear stress.

Contents

A typical parallel-plate flow chamber consists of a polycarbonate distributor, a silicon gasket, and a glass coverslip. The distributor, forming one side of the parallel-plate flow chamber, includes inlet port, outlet port, and a vacuum slot. The thickness of the gasket determines the height of the flow path. The glass coverslip forms another side of the parallel-plate flow chamber and can be coated with extracellular matrix (ECM) proteins, vascular cells, or biomaterials of interest. A vacuum forms a seal to hold these three parts and ensures a uniform channel height. [1]

Typically, the fluid enters one side of the chamber and leaves from an opposite side. The upper plate is usually transparent while the bottom is a prepared surface on which the cells have been cultured for a predetermined period. Cell behavior is viewed with either transmitted or reflective light microscope.

Equation

Within the chamber, fluid flow creates shear stress () at the chamber wall, and a typical equation describing this relationship as a function of flow rate, Q, and chamber height, h, can be derived from Navier-Stokes equations and continuity equation:

Parallel Plate Flow Chamber PPFC.png
Parallel Plate Flow Chamber

With the assumptions such as Newtonian Fluid, Incompressible, Laminar Flow and no slip boundary conditions, Navier-Stokes equations simplifies to:

Solving the first differential equation will provide:

Solving the second differential equation for no slip boundary condition the velocity profile is given by:

This can then be used in continuity equation that states:

Solving this integral will output:

When solving the equation for the change in pressure and plugging it into the first differential equation the shear stress can be calculated for the parallel plate flow chamber.

In which μ is the dynamic viscosity, and w the width of the flow chamber. In these methods, the shear stresses exerted on the cells are assumed approximately equal to the chamber wall shear stresses since cell height is approximately two orders of magnitude less than the chamber.

Advantages

The parallel-plate flow chamber, in its original design, is capable of producing well-defined wall shear-stress in the physiological range of 0.01-30 dyn/cm2. Shear stress is generated by flowing fluid (e.g., anticoagulated whole blood or isolated cell suspensions) through the chamber over the immobilized substrate under controlled kinematic conditions using a syringe pump. The advantages of the parallel-plate flow chamber are:

1. It makes possible study of the effects of constant shear-stress on cells over a defined time-period.

2. The device is simple in design, assembly, and operation.

3. The cells can be grown under flow conditions, and can be observed under a microscope, or visualized in real time, utilizing video microscopy., [2] [3]

PPFC Design

The initial design of the parallel flow chamber is based upon that described by Hochmuth and colleagues to study red blood cells. The parallel plate flow chamber was used in early studies on neutrophils by Wikinson et al. and Forrestor et al. to study their adhesive characteristic on absorbed plasma proteins. Lawrence et al. described one of the first parallel flow chamber assays to study neutrophil adhesion to endothelium. Since these earlier studies, numerous researchers have utilized the parallel plate flow chamber and modified versions of it to examine the dynamics of neutrophil adhesion to various substrates, including endothelial cells, platelets, leukocytes, transfected cell lines, and purified molecules. [4]

Application

The parallel-plate flow chamber is a widely used piece of equipment for studying cellular mechanics on the benchtop. Many researchers used parallel-plate flow chambers to investigate the dynamic adhesion between leukocytes (white blood cells) and endothelial cells (blood vessel lining cells) under definite shear stress. [5] In particular, some studies have been carried out to study leukocyte receptor-ligand interactions. [6] Interactions between cell receptors (selectins and/or integrins) and their ligands mediate rolling and are believed to play an important role in leukocyte adhesion. [7] Moreover, many researchers used parallel-plate flow chambers to provide shear stress and to mimic the environment of cancer cell growth outside of the body. [8] It is a versatile tool in understanding the mechanisms of proliferation, adhesion, and metastasis of cancer cells. Parallel plate flow chambers are widely used also for drug testing in the cellular chemotaxis assay [9] and for novel targeted drug delivery systems based on leukocyte-endothelium adhesion processes.

Related Research Articles

<span class="mw-page-title-main">Navier–Stokes equations</span> Equations describing the motion of viscous fluid substances

The Navier–Stokes equations are partial differential equations which describe the motion of viscous fluid substances. They were named after French engineer and physicist Claude-Louis Navier and the Irish physicist and mathematician George Gabriel Stokes. They were developed over several decades of progressively building the theories, from 1822 (Navier) to 1842–1850 (Stokes).

<span class="mw-page-title-main">Boundary layer</span> Layer of fluid in the immediate vicinity of a bounding surface

In physics and fluid mechanics, a boundary layer is the thin layer of fluid in the immediate vicinity of a bounding surface formed by the fluid flowing along the surface. The fluid's interaction with the wall induces a no-slip boundary condition. The flow velocity then monotonically increases above the surface until it returns to the bulk flow velocity. The thin layer consisting of fluid whose velocity has not yet returned to the bulk flow velocity is called the velocity boundary layer.

A Newtonian fluid is a fluid in which the viscous stresses arising from its flow are at every point linearly correlated to the local strain rate — the rate of change of its deformation over time. Stresses are proportional to the rate of change of the fluid's velocity vector.

A continuity equation or transport equation is an equation that describes the transport of some quantity. It is particularly simple and powerful when applied to a conserved quantity, but it can be generalized to apply to any extensive quantity. Since mass, energy, momentum, electric charge and other natural quantities are conserved under their respective appropriate conditions, a variety of physical phenomena may be described using continuity equations.

<span class="mw-page-title-main">Shear stress</span> Component of stress coplanar with a material cross section

Shear stress is the component of stress coplanar with a material cross section. It arises from the shear force, the component of force vector parallel to the material cross section. Normal stress, on the other hand, arises from the force vector component perpendicular to the material cross section on which it acts.

In physics and engineering, a constitutive equation or constitutive relation is a relation between two or more physical quantities that is specific to a material or substance or field, and approximates its response to external stimuli, usually as applied fields or forces. They are combined with other equations governing physical laws to solve physical problems; for example in fluid mechanics the flow of a fluid in a pipe, in solid state physics the response of a crystal to an electric field, or in structural analysis, the connection between applied stresses or loads to strains or deformations.

<span class="mw-page-title-main">Shear modulus</span> Ratio of shear stress to shear strain

In materials science, shear modulus or modulus of rigidity, denoted by G, or sometimes S or μ, is a measure of the elastic shear stiffness of a material and is defined as the ratio of shear stress to the shear strain:

In physics, shear rate is the rate at which a progressive shear strain is applied to some material, causing shearing to the material.

In fluid dynamics, Couette flow is the flow of a viscous fluid in the space between two surfaces, one of which is moving tangentially relative to the other. The relative motion of the surfaces imposes a shear stress on the fluid and induces flow. Depending on the definition of the term, there may also be an applied pressure gradient in the flow direction.

<span class="mw-page-title-main">Stokes flow</span> Type of fluid flow

Stokes flow, also named creeping flow or creeping motion, is a type of fluid flow where advective inertial forces are small compared with viscous forces. The Reynolds number is low, i.e. . This is a typical situation in flows where the fluid velocities are very slow, the viscosities are very large, or the length-scales of the flow are very small. Creeping flow was first studied to understand lubrication. In nature, this type of flow occurs in the swimming of microorganisms and sperm. In technology, it occurs in paint, MEMS devices, and in the flow of viscous polymers generally.

Fluid mechanics is the branch of physics concerned with the mechanics of fluids and the forces on them. It has applications in a wide range of disciplines, including mechanical, aerospace, civil, chemical, and biomedical engineering, as well as geophysics, oceanography, meteorology, astrophysics, and biology.

In physics and fluid mechanics, a Blasius boundary layer describes the steady two-dimensional laminar boundary layer that forms on a semi-infinite plate which is held parallel to a constant unidirectional flow. Falkner and Skan later generalized Blasius' solution to wedge flow, i.e. flows in which the plate is not parallel to the flow.

The derivation of the Navier–Stokes equations as well as their application and formulation for different families of fluids, is an important exercise in fluid dynamics with applications in mechanical engineering, physics, chemistry, heat transfer, and electrical engineering. A proof explaining the properties and bounds of the equations, such as Navier–Stokes existence and smoothness, is one of the important unsolved problems in mathematics.

Hele-Shaw flow is defined as flow taking place between two parallel flat plates separated by a narrow gap satisfying certain conditions, named after Henry Selby Hele-Shaw, who studied the problem in 1898. Various problems in fluid mechanics can be approximated to Hele-Shaw flows and thus the research of these flows is of importance. Approximation to Hele-Shaw flow is specifically important to micro-flows. This is due to manufacturing techniques, which creates shallow planar configurations, and the typically low Reynolds numbers of micro-flows.

In nonideal fluid dynamics, the Hagen–Poiseuille equation, also known as the Hagen–Poiseuille law, Poiseuille law or Poiseuille equation, is a physical law that gives the pressure drop in an incompressible and Newtonian fluid in laminar flow flowing through a long cylindrical pipe of constant cross section. It can be successfully applied to air flow in lung alveoli, or the flow through a drinking straw or through a hypodermic needle. It was experimentally derived independently by Jean Léonard Marie Poiseuille in 1838 and Gotthilf Heinrich Ludwig Hagen, and published by Hagen in 1839 and then by Poiseuille in 1840–41 and 1846. The theoretical justification of the Poiseuille law was given by George Stokes in 1845.

The Herschel–Bulkley fluid is a generalized model of a non-Newtonian fluid, in which the strain experienced by the fluid is related to the stress in a complicated, non-linear way. Three parameters characterize this relationship: the consistency k, the flow index n, and the yield shear stress . The consistency is a simple constant of proportionality, while the flow index measures the degree to which the fluid is shear-thinning or shear-thickening. Ordinary paint is one example of a shear-thinning fluid, while oobleck provides one realization of a shear-thickening fluid. Finally, the yield stress quantifies the amount of stress that the fluid may experience before it yields and begins to flow.

<span class="mw-page-title-main">Viscosity</span> Resistance of a fluid to shear deformation

The viscosity of a fluid is a measure of its resistance to deformation at a given rate. For liquids, it corresponds to the informal concept of "thickness": for example, syrup has a higher viscosity than water. Viscosity is defined scientifically as a force multiplied by a time divided by an area. Thus its SI units are newton-seconds per square meter, or pascal-seconds.

In fluid mechanics, dynamic similarity is the phenomenon that when there are two geometrically similar vessels with the same boundary conditions and the same Reynolds and Womersley numbers, then the fluid flows will be identical. This can be seen from inspection of the underlying Navier-Stokes equation, with geometrically similar bodies, equal Reynolds and Womersley Numbers the functions of velocity (u’,v’,w’) and pressure (P’) for any variation of flow.

<span class="mw-page-title-main">Falkner–Skan boundary layer</span> Boundary layer that forms on a wedge

In fluid dynamics, the Falkner–Skan boundary layer describes the steady two-dimensional laminar boundary layer that forms on a wedge, i.e. flows in which the plate is not parallel to the flow. It is also representative of flow on a flat plate with an imposed pressure gradient along the plate length, a situation often encountered in wind tunnel flow. It is a generalization of the flat plate Blasius boundary layer in which the pressure gradient along the plate is zero.

In fluid mechanics, the thin-film equation is a partial differential equation that approximately predicts the time evolution of the thickness h of a liquid film that lies on a surface. The equation is derived via lubrication theory which is based on the assumption that the length-scales in the surface directions are significantly larger than in the direction normal to the surface. In the non-dimensional form of the Navier-Stokes equation the requirement is that terms of order ε2 and ε2Re are negligible, where ε ≪ 1 is the aspect ratio and Re is the Reynolds number. This significantly simplifies the governing equations. However, lubrication theory, as the name suggests, is typically derived for flow between two solid surfaces, hence the liquid forms a lubricating layer. The thin-film equation holds when there is a single free surface. With two free surfaces, the flow must be treated as a viscous sheet.

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  6. Taite, Lakeshia J.; Rowland, Maude L.; Ruffino, Katie A.; Smith, Bryan R. E.; Lawrence, Michael B.; West, Jennifer L. "Bioactive Hydrogel Substrates: Probing Leukocyte Receptor–Ligand Interactions in Parallel Plate Flow Chamber Studies". Annals of Biomedical Engineering. Vol. 34 Issue 11. 2006-11-02
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