
Conservation and restoration of rail vehicles aims to preserve historic rail vehicles. It may concern trains that have been removed from service and later restored to their past condition, or have never been removed from service, like UP 844, the only U.S. steam locomotive to never be retired. They are often operated in present-day service as moving examples of living history, as opposed to static exhibits. The majority of restored trains are operated at heritage railways and railway museums, although they can also be found on the main lines or branch lines of the commercial working railway, operated by specialist railtour companies or museum groups.
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The Minnesota Transportation Museum is a transportation museum in Saint Paul, Minnesota, United States.
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The Connecticut Trolley Museum, also known as the Warehouse Point Trolley Museum, is the oldest incorporated museum dedicated to electric railroading in the United States. Founded in October 1940, the museum is located in East Windsor, Connecticut and is open to the public April through December. The museum features static and moving displays, and self-guided tours of the state's trolley history.
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Old Pueblo Trolley is a non-profit, educational corporation based in Tucson, in the U.S. state of Arizona, that is dedicated to the preservation of Arizona's mass transit history. The name also commonly refers to the heritage streetcar line which OPT began operating in 1993, on which service is currently indefinitely suspended. OPT consists of three divisions that each fill a specific role in preserving the state's mass transit history. The divisions are the Street Railway Division, Motor Bus Division and the Museum Division.
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The Fort Collins Municipal Railway operated streetcars in Fort Collins, Colorado, from 1919 until 1951. Since 1984, a section of one of the former routes has been in operation as a seasonal heritage streetcar service, under the same name, running primarily on Spring and Summer weekends. The heritage service is operated by volunteers from the Fort Collins Municipal Railway Society (FCMRS). The streetcar in use on the heritage line, Birney "Safety" Streetcar No. 21, is listed on the National Register of Historic Places.
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The M-Line Trolley is a heritage streetcar line in the Uptown neighborhood of Dallas, Texas. The trolley line, which has been in service since 1989, is notable for its use of restored historic streetcar vehicles, as opposed to modern replicas.
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The Shore Line Trolley Museum is a trolley museum located in East Haven, Connecticut. Incorporated in 1945, it is the oldest continuously operating trolley museum in the United States. The museum includes exhibits on trolley history in the visitors' center and offers rides on restored trolleys along its 1.5 mi (2.4 km) track as the Branford Electric Railway. In addition to trolleys, the museum also operates restored subway cars, a small number of both trolleybuses and conventional buses.
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The East Troy Electric Railroad is an interurban heritage railroad owned and operated by the East Troy Railroad Museum. Passenger excursions run on a 7-mile (11 km) stretch of track from East Troy to Mukwonago, Wisconsin.

The Pennsylvania Trolley Museum is a museum in Washington, Pennsylvania, dedicated to the operation and preservation of streetcars and trolleys. The museum primarily contains historic trolleys from Pennsylvania, but its collection includes examples from nearby Toledo, New Orleans, and even an open-sided car from Brazil. Many have been painstakingly restored to operating condition. Other unique cars either awaiting restoration or that are incompatible with the 5' 2-1/2" Pennsylvania trolley gauge track are on display in a massive trolley display building. Notable examples of static display include a J.G. Brill “Brilliner” car, locomotives, and a horse car from the early days of Pittsburgh’s public transit systems.
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The Fort Smith Trolley Museum is a streetcar and railroad museum in Fort Smith, in the U.S. state of Arkansas, which includes an operating heritage streetcar line. The museum opened in 1985, and operation of its streetcar line began in 1991. Four vehicles in its collection, a streetcar and three steam locomotives, are listed on the National Register of Historic Places (NRHP). The now approximately three-quarters-mile-long (1.2 km) streetcar line also passes four NRHP-listed sites, including the Fort Smith National Historic Site, the Fort Smith National Cemetery, the West Garrison Avenue Historic District and the 1907 Atkinson-Williams Warehouse Building, which now houses the Fort Smith Museum of History.
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The National Capital Trolley Museum (NCTM) is a 501(c)(3) nonprofit organization that operates historic street cars, trolleys and trams for the public on a regular schedule. Located in Montgomery County, Maryland, the museum's primary mission is to preserve and interpret the history of the electric street and interurban railways of the National Capital region.
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The Phoenix Street Railway provided streetcar service in Phoenix, Arizona, United States, from 1888 to 1948. The motto was "Ride a Mile and Smile the While."
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The Virginia Museum of Transportation (VMT) is a museum in Downtown Roanoke, Virginia, that is devoted to the topic of transportation.
The Boston Street Railway Association (BSRA) is a non-profit organization in Boston, Massachusetts, whose central objective is preserving transportation history in Boston and throughout New England. They host monthly membership meetings, publish a bimonthly transit news magazine, and are restoring an ex-Boston Type 5 streetcar. They also regularly publish their own books and materials, as well as organize fan trips on Boston's MBTA and to the Seashore Trolley Museum in Kennebunkport, Maine. Funding for the organization is provided primarily through tax-deductible member and individual donations, as well as through grants and donations from other organizations and groups.
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The New York Museum of Transportation (NYMT), founded in 1975, is a non-profit organization located at 6393 East River Road, in the Rochester suburb of Rush. A private rail line built by volunteers connects NYMT with the Rochester & Genesee Valley Railroad Museum, over a distance of two miles. This demonstration railway allows both museums to offer train rides with their collections of vintage railroad equipment. NYMT operates the only electric trolley ride in New York State, not to be confused with the similarly named Trolley Museum of New York located in Kingston, New York.
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The Southeastern Railway Museum is a railroad museum located in Duluth, Georgia, in suburban Atlanta.
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The Lake Shore Electric Railway was an attempt to start an electric railway museum in Cleveland, Ohio.
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The Southern California Railway Museum, formerly known as the Orange Empire Railway Museum, is a railroad museum in Perris, California, United States. It was founded in 1956 at Griffith Park in Los Angeles before moving to the former Pinacate Station as the "Orange Empire Trolley Museum" in 1958. It was renamed "Orange Empire Railway Museum" in 1975 after merging with a museum then known as the California Southern Railroad Museum, and adopted its current name in 2019. The museum also operates a heritage railroad on the museum grounds.
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The Manchester Street Railway was a light interurban railway that ran from Manchester to Nashua, New Hampshire.