SF3A3

SF3A3
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	2DT7
Identifiers
Aliases	SF3A3 , PRP9, PRPF9, SAP61, SF3a60, splicing factor 3a subunit 3
External IDs	OMIM: 605596 MGI: 1922312 HomoloGene: 4949 GeneCards: SF3A3
Gene location (Human)
Chr.	Chromosome 1 (human)
End	37,990,075 bp
Gene location (Mouse)
Chr.	Chromosome 4 (mouse)
End	124,626,253 bp
RNA expression pattern
	Top expressed in
	testicle; ; sural nerve; ; lung; ; Achilles tendon; ; kidney; ; thymus; ; stomach;
	More reference expression data
	More reference expression data
Gene ontology
Molecular function	zinc ion binding ; GO:0001948 protein binding ; metal ion binding ; nucleic acid binding ; RNA binding ;
Cellular component	nuclear speck ; catalytic step 2 spliceosome ; spliceosomal complex ; nucleus ; nucleoplasm ; U2 snRNP ; U2-type precatalytic spliceosome ;
Biological process	RNA splicing, via transesterification reactions ; mRNA splicing, via spliceosome ; mRNA processing ; mRNA 3'-splice site recognition ; RNA splicing ; U2-type prespliceosome assembly ;
	Sources:Amigo / QuickGO
Orthologs
	10946
	75062
	ENSG00000183431
	ENSMUSG00000028902
	Q12874
	Q9D554
	NM_006802 ; NM_001320830
	NM_029157
	NP_001307759 ; NP_006793
	NP_083433
	Wikidata
View/Edit Human	View/Edit Mouse

Last updated April 09, 2022

Splicing factor 3A subunit 3 is a protein that in humans is encoded by the SF3A3 gene.^[5]^[6]^[7]

This gene encodes subunit 3 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 3 interacts with subunit 1 through its amino-terminus while the zinc finger domain of subunit 3 plays a role in its binding to the 15S U2 snRNP. This gene has a pseudogene on chromosome 20.^[7]

Interactions

SF3A3 has been shown to interact with SF3A1.^[6]^[8]

Related Research Articles

A spliceosome is a large ribonucleoprotein (RNP) complex found primarily within the nucleus of eukaryotic cells. The spliceosome is assembled from small nuclear RNAs (snRNA) and numerous proteins. Small nuclear RNA (snRNA) molecules bind to specific proteins to form a small nuclear ribonucleoprotein complex, which in turn combines with other snRNPs to form a large ribonucleoprotein complex called a spliceosome. The spliceosome removes introns from a transcribed pre-mRNA, a type of primary transcript. This process is generally referred to as splicing. An analogy is a film editor, who selectively cuts out irrelevant or incorrect material from the initial film and sends the cleaned-up version to the director for the final cut.

U4 spliceosomal RNA Non-coding RNA component of the spliceosome

The U4 small nuclear Ribo-Nucleic Acid is a non-coding RNA component of the major or U2-dependent spliceosome – a eukaryotic molecular machine involved in the splicing of pre-messenger RNA (pre-mRNA). It forms a duplex with U6, and with each splicing round, it is displaced from the U6 snRNA in an ATP-dependent manner, allowing U6 to re-fold and create the active site for splicing catalysis. A recycling process involving protein Brr2 releases U4 from U6, while protein Prp24 re-anneals U4 and U6. The crystal structure of a 5′ stem-loop of U4 in complex with a binding protein has been solved.

Splicing factor U2AF 65 kDa subunit is a protein that in humans is encoded by the U2AF2 gene.

Splicing factor U2AF 35 kDa subunit is a protein that in humans is encoded by the U2AF1 gene.

Splicing factor 3 subunit 1 is a protein that in humans is encoded by the SF3A1 gene.

Heterogeneous nuclear ribonucleoprotein F is a protein that in humans is encoded by the HNRNPF gene.

Splicing factor 1 also known as zinc finger protein 162 (ZFM162) is a protein that in humans is encoded by the SF1 gene.

Splicing factor 3B subunit 1 is a protein that in humans is encoded by the SF3B1 gene.

U5 small nuclear ribonucleoprotein 200 kDa helicase is an enzyme that in humans is encoded by the SNRNP200 gene.

Splicing factor 3A subunit 2 is a protein that in humans is encoded by the SF3A2 gene.

Transcription elongation regulator 1, also known as TCERG1, is a protein which in humans is encoded by the TCERG1 gene.

Splicing factor 3B subunit 2 is a protein that in humans is encoded by the SF3B2 gene.

Splicing factor 3B subunit 4 is a protein that in humans is encoded by the SF3B4 gene.

Splicing factor 3B subunit 3 is a protein that in humans is encoded by the SF3B3 gene.

Pre-mRNA-processing factor 6 is a protein that in humans is encoded by the PRPF6 gene.

U2 small nuclear ribonucleoprotein B is a protein that in humans is encoded by the SNRPB2 gene.

Serine/threonine-protein kinase PRP4 homolog is an enzyme that in humans is encoded by the PRPF4B gene.

U4/U6 small nuclear ribonucleoprotein Prp4 is a protein that in humans is encoded by the PRPF4 gene. The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors. PRPF4 is 1 of several proteins that associate with U4 and U6 snRNPs.[supplied by OMIM]

Splicing factor 3B, 14 kDa subunit, also known as SF3B14, is a human gene.

Probable ATP-dependent RNA helicase DDX23 is an enzyme that in humans is encoded by the DDX23 gene.

References

1 2 3 GRCh38: Ensembl release 89: ENSG00000183431 - Ensembl, May 2017
1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000028902 - Ensembl, May 2017
↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ Kramer A; Legrain P; Mulhauser F; Groning K; Brosi R; Bilbe G (February 1995). "Splicing factor SF3a60 is the mammalian homologue of PRP9 of S.cerevisiae: the conserved zinc finger-like motif is functionally exchangeable in vivo". Nucleic Acids Res. 22 (24): 5223–8. doi:10.1093/nar/22.24.5223. PMC 332064 . PMID 7816610.
1 2 Chiara MD; Champion-Arnaud P; Buvoli M; Nadal-Ginard B; Reed R (August 1994). "Specific protein-protein interactions between the essential mammalian spliceosome-associated proteins SAP 61 and SAP 114". Proc Natl Acad Sci U S A. 91 (14): 6403–7. Bibcode:1994PNAS...91.6403C. doi: 10.1073/pnas.91.14.6403 . PMC 44210 . PMID 8022796.
1 2 "Entrez Gene: SF3A3 splicing factor 3a, subunit 3, 60kDa".
↑ Nesic, D; Krämer A (October 2001). "Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation". Mol. Cell. Biol. United States. 21 (19): 6406–17. doi:10.1128/MCB.21.19.6406-6417.2001. ISSN 0270-7306. PMC 99788 . PMID 11533230.

Maruyama K; Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Bonaldo MF; Lennon G; Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi: 10.1101/gr.6.9.791 . PMID 8889548.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Neubauer G, King A, Rappsilber J, et al. (1998). "Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex". Nat. Genet. 20 (1): 46–50. doi:10.1038/1700. PMID 9731529. S2CID 585778.
Das R; Zhou Z; Reed R (2000). "Functional association of U2 snRNP with the ATP-independent spliceosomal complex E". Mol. Cell. 5 (5): 779–87. doi: 10.1016/S1097-2765(00)80318-4 . PMID 10882114.
Will CL, Schneider C, MacMillan AM, et al. (2001). "A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site". EMBO J. 20 (16): 4536–46. doi:10.1093/emboj/20.16.4536. PMC 125580 . PMID 11500380.
Nesic D; Krämer A (2001). "Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation". Mol. Cell. Biol. 21 (19): 6406–17. doi:10.1128/MCB.21.19.6406-6417.2001. PMC 99788 . PMID 11533230.
Jurica MS, Licklider LJ, Gygi SR, et al. (2002). "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis". RNA. 8 (4): 426–39. doi:10.1017/S1355838202021088. PMC 1370266 . PMID 11991638.
Will CL, Urlaub H, Achsel T, et al. (2002). "Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein". EMBO J. 21 (18): 4978–88. doi:10.1093/emboj/cdf480. PMC 126279 . PMID 12234937.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC 139241 . PMID 12477932.
Dubois T; Zemlickova E; Howell S; Aitken A (2003). "Centaurin-alpha 1 associates in vitro and in vivo with nucleolin". Biochem. Biophys. Res. Commun. 301 (2): 502–8. doi:10.1016/S0006-291X(02)03010-3. PMID 12565890.
Li J, Hawkins IC, Harvey CD, et al. (2003). "Regulation of alternative splicing by SRrp86 and its interacting proteins". Mol. Cell. Biol. 23 (21): 7437–47. doi:10.1128/MCB.23.21.7437-7447.2003. PMC 207616 . PMID 14559993.
Nesic D; Tanackovic G; Krämer A (2005). "A role for Cajal bodies in the final steps of U2 snRNP biogenesis". J. Cell Sci. 117 (Pt 19): 4423–33. doi: 10.1242/jcs.01308 . PMID 15316075.
Lin KT; Lu RM; Tarn WY (2004). "The WW domain-containing proteins interact with the early spliceosome and participate in pre-mRNA splicing in vivo". Mol. Cell. Biol. 24 (20): 9176–85. doi:10.1128/MCB.24.20.9176-9185.2004. PMC 517884 . PMID 15456888.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928 . PMID 15489334.
Rush J, Moritz A, Lee KA, et al. (2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nat. Biotechnol. 23 (1): 94–101. doi:10.1038/nbt1046. PMID 15592455. S2CID 7200157.
Andersen JS, Lam YW, Leung AK, et al. (2005). "Nucleolar proteome dynamics". Nature. 433 (7021): 77–83. Bibcode:2005Natur.433...77A. doi:10.1038/nature03207. PMID 15635413. S2CID 4344740.
Tanackovic G; Krämer A (2005). "Human splicing factor SF3a, but not SF1, is essential for pre-mRNA splicing in vivo". Mol. Biol. Cell. 16 (3): 1366–77. doi:10.1091/mbc.E04-11-1034. PMC 551499 . PMID 15647371.

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[refGRCh38Ensembl-1] 1 2 3 GRCh38: Ensembl release 89: ENSG00000183431 - Ensembl, May 2017

[refGRCm38Ensembl-2] 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000028902 - Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid7816610-5] Kramer A; Legrain P; Mulhauser F; Groning K; Brosi R; Bilbe G (February 1995). "Splicing factor SF3a60 is the mammalian homologue of PRP9 of S.cerevisiae: the conserved zinc finger-like motif is functionally exchangeable in vivo". Nucleic Acids Res. 22 (24): 5223–8. doi:10.1093/nar/22.24.5223. PMC 332064 . PMID 7816610.

[pmid8022796-6] 1 2 Chiara MD; Champion-Arnaud P; Buvoli M; Nadal-Ginard B; Reed R (August 1994). "Specific protein-protein interactions between the essential mammalian spliceosome-associated proteins SAP 61 and SAP 114". Proc Natl Acad Sci U S A. 91 (14): 6403–7. Bibcode:1994PNAS...91.6403C. doi: 10.1073/pnas.91.14.6403 . PMC 44210 . PMID 8022796.

[entrez-7] 1 2 "Entrez Gene: SF3A3 splicing factor 3a, subunit 3, 60kDa".

[pmid11533230-8] Nesic, D; Krämer A (October 2001). "Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation". Mol. Cell. Biol. United States. 21 (19): 6406–17. doi:10.1128/MCB.21.19.6406-6417.2001. ISSN 0270-7306. PMC 99788 . PMID 11533230.

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SF3A3

Contents

Interactions

Related Research Articles

References

Further reading