SPPL2A

SPPL2A
Identifiers
Aliases	SPPL2A , IMP3, PSL2, signal peptide peptidase like 2A, IMD86
External IDs	OMIM: 608238; MGI: 1913802; HomoloGene: 36411; GeneCards: SPPL2A; OMA:SPPL2A - orthologs
Gene location (Human) Chr. Chromosome 15 (human) [1] Band 15q21.2 Start 50,702,266 bp [1] End 50,765,709 bp [1]
Gene location (Mouse) Chr. Chromosome 2 (mouse) [2] Band 2|2 F1 Start 126,890,391 bp [2] End 126,933,235 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in mucosa of ileum secondary oocyte tibialis anterior muscle Skeletal muscle tissue of biceps brachii vastus lateralis muscle Skeletal muscle tissue of rectus abdominis deltoid muscle cartilage tissue rectum mucosa of colon Top expressed in body of femur submandibular gland ascending aorta aortic valve calvaria stroma of bone marrow left lobe of liver knee joint mesenteric lymph nodes muscle of thigh More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein homodimerization activity aspartic-type endopeptidase activity peptidase activity protein binding hydrolase activity aspartic endopeptidase activity, intramembrane cleaving Cellular component integral component of membrane endosome late endosome membrane late endosome membrane intracellular membrane-bounded organelle integral component of cytoplasmic side of endoplasmic reticulum membrane lysosomal membrane Golgi-associated vesicle membrane lysosome integral component of lumenal side of endoplasmic reticulum membrane extracellular exosome plasma membrane Biological process membrane protein proteolysis proteolysis membrane protein ectodomain proteolysis membrane protein intracellular domain proteolysis regulation of immune response regulation of tumor necrosis factor-mediated signaling pathway Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 84888 66552 Ensembl ENSG00000138600 ENSMUSG00000027366 UniProt Q8TCT8 Q9JJF9 RefSeq (mRNA) NM_032802 NM_023220 RefSeq (protein) NP_116191 NP_075709 Location (UCSC) Chr 15: 50.7 – 50.77 Mb Chr 2: 126.89 – 126.93 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Signal peptide peptidase-like 2A, also known as SPPL2A, is a human gene. ^[5]

Function

This gene is a member of the signal peptide peptidase-like protease (SPPL) family and encodes a lysosomal/late endosomal membrane protein ^[6] with the conserved active site motifs 'YD' and 'GxGD' in adjacent transmembrane domains (TMDs). This protein plays a role in innate and adaptive immunity by cleaving TNFα in activated dendritic cells. ^{[7] [8]} A pseudogene of this gene also lies on chromosome 15. ^[5]

References

1. GRCh38: Ensembl release 89: ENSG00000138600 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000027366 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: SPPL2A signal peptide peptidase-like 2A".
6. Behnke J, Schneppenheim J, Koch-Nolte F, Haag F, Saftig P, Schröder B (2011). "Signal-peptide-peptidase-like 2a (SPPL2a) is targeted to lysosomes/late endosomes by a tyrosine motif in its C-terminal tail". FEBS Letters. 585 (19): 2951–7. doi: 10.1016/j.febslet.2011.08.043 . PMID   21896273.
7. Friedmann E, Hauben E, Maylandt K, et al. (2006). "SPPL2a and SPPL2b promote intramembrane proteolysis of TNFalpha in activated dendritic cells to trigger IL-12 production". Nat. Cell Biol. 8 (8): 843–8. doi:10.1038/ncb1440. PMID   16829952. S2CID   129089.
8. Fluhrer R, Grammer G, Israel L, et al. (2006). "A gamma-secretase-like intramembrane cleavage of TNFalpha by the GxGD aspartyl protease SPPL2b" (PDF). Nat. Cell Biol. 8 (8): 894–6. doi:10.1038/ncb1450. PMID   16829951. S2CID   23712486.

Further reading

• Weihofen A, Binns K, Lemberg MK, et al. (2002). "Identification of signal peptide peptidase, a presenilin-type aspartic protease". Science. 296 (5576): 2215–8. Bibcode:2002Sci...296.2215W. doi:10.1126/science.1070925. PMID   12077416. S2CID   45633906.
• Grigorenko AP, Moliaka YK, Korovaitseva GI, Rogaev EI (2002). "Novel class of polytopic proteins with domains associated with putative protease activity". Biochemistry Mosc. 67 (7): 826–35. doi:10.1023/A:1016365227942. PMID   12139484. S2CID   11785597.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Granneman S, Gallagher JE, Vogelzangs J, et al. (2003). "The human Imp3 and Imp4 proteins form a ternary complex with hMpp10, which only interacts with the U3 snoRNA in 60–80S ribonucleoprotein complexes". Nucleic Acids Res. 31 (7): 1877–87. doi:10.1093/nar/gkg300. PMC   152815 . PMID   12655004.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi: 10.1038/ng1285 . PMID   14702039.
• Friedmann E, Lemberg MK, Weihofen A, et al. (2005). "Consensus analysis of signal peptide peptidase and homologous human aspartic proteases reveals opposite topology of catalytic domains compared with presenilins". J. Biol. Chem. 279 (49): 50790–8. doi: 10.1074/jbc.M407898200 . hdl: 2262/89311 . PMID   15385547.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC   528928 . PMID   15489334.
• Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID   16189514. S2CID   4427026.
• Otsuki T, Ota T, Nishikawa T, et al. (2007). "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries". DNA Res. 12 (2): 117–26. doi: 10.1093/dnares/12.2.117 . PMID   16303743.
• Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: Large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC   1356129 . PMID   16344560.
• Kirkin V, Cahuzac N, Guardiola-Serrano F, et al. (2007). "The Fas ligand intracellular domain is released by ADAM10 and SPPL2a cleavage in T-cells". Cell Death Differ. 14 (9): 1678–87. doi: 10.1038/sj.cdd.4402175 . PMID   17557115.