SSX2IP

SSX2IP
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1T2M, 1XZ9, 2AIN, 2EXG
Identifiers
Aliases	SSX2IP , ADIP, synovial sarcoma, X breakpoint 2 interacting protein, SSX family member 2 interacting protein, hMsd1
External IDs	OMIM: 608690; MGI: 2139150; HomoloGene: 8522; GeneCards: SSX2IP; OMA:SSX2IP - orthologs
Gene location (Human) Chr. Chromosome 1 (human) [1] Band 1p22.3 Start 84,643,706 bp [1] End 84,690,803 bp [1]
Gene location (Mouse) Chr. Chromosome 3 (mouse) [2] Band 3|3 H2 Start 146,110,397 bp [2] End 146,145,899 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in left testis right testis middle temporal gyrus sperm Brodmann area 23 jejunal mucosa Brodmann area 46 endothelial cell duodenum secondary oocyte Top expressed in Ileal epithelium seminiferous tubule spermatid olfactory epithelium lens spermatocyte fetal liver hematopoietic progenitor cell CA3 field retinal pigment epithelium entorhinal cortex More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein binding protein domain specific binding Cellular component cytoplasm microtubule organizing center adherens junction cell junction cell projection cytoskeleton cell leading edge nucleus ciliary basal body centriolar satellite protein-containing complex Biological process regulation of cell motility intraciliary transport involved in cilium assembly regulation of Rac protein signal transduction cell adhesion cell projection organization cilium assembly centrosome cycle Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 117178 99167 Ensembl ENSG00000117155 ENSMUSG00000036825 UniProt Q9Y2D8 Q8VC66 RefSeq (mRNA) NM_001166293 NM_001166294 NM_001166295 NM_001166417 NM_014021 NM_001253768 NM_001253769 NM_001253770 NM_138744 NM_001355661 RefSeq (protein) NP_001159765 NP_001159766 NP_001159767 NP_001159889 NP_054740 NP_001240697 NP_001240698 NP_001240699 NP_620083 NP_001342590 Location (UCSC) Chr 1: 84.64 – 84.69 Mb Chr 3: 146.11 – 146.15 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Afadin- and alpha-actinin-binding protein is a protein that in humans is encoded by the SSX2IP gene. ^[5] It has been shown that it functions together with WDR8 in centrosome maturation, ensuring proper spindle length and orientation. ^{[6] [7] [8] [9] [10]} The SSX2IP-WDR8 complex additionally promotes ciliary vesicle docking during ciliogenesis. ^{[11] [12]}

Interactions

SSX2IP has been initially described as a protein to interact with MLLT4 ^[13] and Actinin, alpha 1. ^[13] In the context of centrosome maturation and ciliogenesis it has been shown to interact with WDR8. ^{[8] [9] [11]}

References

1. GRCh38: Ensembl release 89: ENSG00000117155 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000036825 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: SSX2IP synovial sarcoma, X breakpoint 2 interacting protein".
6. Bärenz F, Inoue D, Yokoyama H, Tegha-Dunghu J, Freiss S, Draeger S, et al. (July 2013). "The centriolar satellite protein SSX2IP promotes centrosome maturation". The Journal of Cell Biology. 202 (1): 81–95. doi:10.1083/jcb.201302122. PMC   3704989 . PMID   23816619.
7. Yukawa M, Ikebe C, Toda T (May 2015). "The Msd1-Wdr8-Pkl1 complex anchors microtubule minus ends to fission yeast spindle pole bodies". The Journal of Cell Biology. 209 (4): 549–62. doi:10.1083/jcb.201412111. PMC   4442821 . PMID   25987607.
8. Inoue D, Stemmer M, Thumberger T, Ruppert T, Bärenz F, Wittbrodt J, Gruss OJ (January 2017). "Expression of the novel maternal centrosome assembly factor Wdr8 is required for vertebrate embryonic mitoses". Nature Communications. 8 14090. Bibcode:2017NatCo...814090I. doi:10.1038/ncomms14090. PMC   5253655 . PMID   28098238.
9. Hori A, Morand A, Ikebe C, Frith D, Snijders AP, Toda T (2015-12-04). "The conserved Wdr8-hMsd1/SSX2IP complex localises to the centrosome and ensures proper spindle length and orientation". Biochemical and Biophysical Research Communications. 468 (1–2): 39–45. doi:10.1016/j.bbrc.2015.10.169. PMC   4669408 . PMID   26545777.
10. Hori A, Peddie CJ, Collinson LM, Toda T (June 2015). "Centriolar satellite- and hMsd1/SSX2IP-dependent microtubule anchoring is critical for centriole assembly". Molecular Biology of the Cell. 26 (11): 2005–19. doi:10.1091/mbc.E14-11-1561. PMC   4472012 . PMID   25833712.
11. Kurtulmus B, Wang W, Ruppert T, Neuner A, Cerikan B, Viol L, Dueñas-Sánchez R, Gruss OJ, Pereira G (February 2016). "WDR8 is a centriolar satellite and centriole-associated protein that promotes ciliary vesicle docking during ciliogenesis". Journal of Cell Science. 129 (3): 621–36. doi: 10.1242/jcs.179713 . PMID   26675238.
12. Klinger M, Wang W, Kuhns S, Bärenz F, Dräger-Meurer S, Pereira G, Gruss OJ (February 2014). "The novel centriolar satellite protein SSX2IP targets Cep290 to the ciliary transition zone". Molecular Biology of the Cell. 25 (4): 495–507. doi:10.1091/mbc.E13-09-0526. PMC   3923641 . PMID   24356449.
13. Asada M, Irie K, Morimoto K, Yamada A, Ikeda W, Takeuchi M, Takai Y (February 2003). "ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions". The Journal of Biological Chemistry. 278 (6): 4103–11. doi: 10.1074/jbc.M209832200 . PMID   12446711.

Further reading

• Nagase T, Ishikawa K, Suyama M, Kikuno R, Hirosawa M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (February 1999). "Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Research. 6 (1): 63–70. doi: 10.1093/dnares/6.1.63 . PMID   10231032.
• Hartley JL, Temple GF, Brasch MA (November 2000). "DNA cloning using in vitro site-specific recombination". Genome Research. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC   310948 . PMID   11076863.
• Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, Böcher M, Blöcker H, Bauersachs S, Blum H, Lauber J, Düsterhöft A, Beyer A, Köhrer K, Strack N, Mewes HW, Ottenwälder B, Obermaier B, Tampe J, Heubner D, Wambutt R, Korn B, Klein M, Poustka A (March 2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Research. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC   311072 . PMID   11230166.
• Simpson JC, Wellenreuther R, Poustka A, Pepperkok R, Wiemann S (September 2000). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Reports. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC   1083732 . PMID   11256614.
• de Bruijn DR, dos Santos NR, Kater-Baats E, Thijssen J, van den Berk L, Stap J, Balemans M, Schepens M, Merkx G, van Kessel AG (July 2002). "The cancer-related protein SSX2 interacts with the human homologue of a Ras-like GTPase interactor, RAB3IP, and a novel nuclear protein, SSX2IP". Genes, Chromosomes & Cancer. 34 (3): 285–98. doi:10.1002/gcc.10073. PMID   12007189. S2CID   11734893.
• Asada M, Irie K, Morimoto K, Yamada A, Ikeda W, Takeuchi M, Takai Y (February 2003). "ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions". The Journal of Biological Chemistry. 278 (6): 4103–11. doi: 10.1074/jbc.M209832200 . PMID   12446711.
• Wiemann S, Arlt D, Huber W, Wellenreuther R, Schleeger S, Mehrle A, Bechtel S, Sauermann M, Korf U, Pepperkok R, Sültmann H, Poustka A (October 2004). "From ORFeome to biology: a functional genomics pipeline". Genome Research. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC   528930 . PMID   15489336.
• Guinn BA, Bland EA, Lodi U, Liggins AP, Tobal K, Petters S, Wells JW, Banham AH, Mufti GJ (October 2005). "Humoral detection of leukaemia-associated antigens in presentation acute myeloid leukaemia". Biochemical and Biophysical Research Communications. 335 (4): 1293–304. doi:10.1016/j.bbrc.2005.08.024. PMID   16112646.
• Mehrle A, Rosenfelder H, Schupp I, del Val C, Arlt D, Hahne F, Bechtel S, Simpson J, Hofmann O, Hide W, Glatting KH, Huber W, Pepperkok R, Poustka A, Wiemann S (January 2006). "The LIFEdb database in 2006". Nucleic Acids Research. 34 (Database issue): D415-8. doi:10.1093/nar/gkj139. PMC   1347501 . PMID   16381901.
• Nousiainen M, Silljé HH, Sauer G, Nigg EA, Körner R (April 2006). "Phosphoproteome analysis of the human mitotic spindle". Proceedings of the National Academy of Sciences of the United States of America. 103 (14): 5391–6. Bibcode:2006PNAS..103.5391N. doi: 10.1073/pnas.0507066103 . PMC   1459365 . PMID   16565220.