Sperm-associated antigen 9

SPAG9
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2W83
Identifiers
Aliases	SPAG9 , CT89, HLC-6, HLC4, JIP-4, JIP4, JLP, PHET, PIG6, HLC6, sperm associated antigen 9
External IDs	OMIM: 605430; MGI: 1918084; HomoloGene: 2954; GeneCards: SPAG9; OMA:SPAG9 - orthologs
Gene location (Human) Chr. Chromosome 17 (human) [1] Band 17q21.33 Start 50,962,174 bp [1] End 51,120,868 bp [1]
Gene location (Mouse) Chr. Chromosome 11 (mouse) [2] Band 11|11 D Start 93,886,917 bp [2] End 94,016,911 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in Achilles tendon ventricular zone sural nerve ganglionic eminence corpus callosum internal globus pallidus inferior ganglion of vagus nerve endothelial cell cartilage tissue subthalamic nucleus Top expressed in otolith organ utricle superior cervical ganglion sciatic nerve trigeminal ganglion substantia nigra mammillary body superior colliculus cerebellar vermis lobe of cerebellum More reference expression data BioGPS More reference expression data
Gene ontology Molecular function JUN kinase binding MAP-kinase scaffold activity mitogen-activated protein kinase p38 binding protein binding kinesin binding signaling receptor complex adaptor activity Cellular component integral component of membrane microtubule organizing center acrosomal vesicle perinuclear region of cytoplasm extracellular exosome cytoplasmic vesicle cytoplasm cytosol lysosomal membrane Biological process positive regulation of muscle cell differentiation positive regulation of cell migration retrograde transport, endosome to Golgi positive regulation of neuron differentiation spermatogenesis negative regulation of protein homodimerization activity striated muscle cell differentiation protein homooligomerization positive regulation of MAPK cascade vesicle-mediated transport lysosome localization Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 9043 70834 Ensembl ENSG00000008294 ENSMUSG00000020859 UniProt O60271 Q58A65 RefSeq (mRNA) NM_001130527 NM_001130528 NM_001251971 NM_003971 NM_172345 NM_001025428 NM_001025429 NM_001025430 NM_001199203 NM_001199204 NM_001199205 NM_027569 NM_001363157 RefSeq (protein) NP_001123999 NP_001124000 NP_001238900 NP_003962 NP_001020599 NP_001020600 NP_001020601 NP_001186132 NP_001186133 NP_001186134 NP_081845 NP_001350086 Location (UCSC) Chr 17: 50.96 – 51.12 Mb Chr 11: 93.89 – 94.02 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

C-jun-amino-terminal kinase-interacting protein 4 is a scaffold protein that in humans is encoded by the SPAG9 gene. ^{[5] [6] [7]}

Function

Extracellular signals are transduced into cells through mitogen-activated protein kinases. The structural organization of these kinases into specific signaling domains is facilitated by scaffolding proteins involved in closely tethering different kinases so that successive phosphorylation events can occur. The protein encoded by this gene is a scaffolding protein that brings together mitogen-activated protein kinases and their transcription factor targets for the activation of specific signaling pathways. This gene which is abundantly expressed in testicular haploid germ cells encodes a protein that is recognized by sperm-agglutinating antibodies and implicated in infertility. ^[7]

Clinical significance

SPAG9 is a potential biomarker for early cervical carcinoma ^[8] bladder cancer, ^[9] and lung cancer. ^[10]

Interactions

SPAG9 has been shown to interact with MAX. ^[11]

References

1. GRCh38: Ensembl release 89: ENSG00000008294 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000020859 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Shankar S, Mohapatra B, Suri A (Feb 1998). "Cloning of a novel human testis mRNA specifically expressed in testicular haploid germ cells, having unique palindromic sequences and encoding a leucine zipper dimerization motif". Biochemical and Biophysical Research Communications. 243 (2): 561–5. doi:10.1006/bbrc.1997.7943. PMID   9480848.
6. Bowman AB, Kamal A, Ritchings BW, Philp AV, McGrail M, Gindhart JG, Goldstein LS (Nov 2000). "Kinesin-dependent axonal transport is mediated by the sunday driver (SYD) protein". Cell. 103 (4): 583–94. doi: 10.1016/S0092-8674(00)00162-8 . PMID   11106729. S2CID   247102.
7. "Entrez Gene: SPAG9 sperm associated antigen 9".
8. Garg M, Kanojia D, Salhan S, Suri S, Gupta A, Lohiya NK, Suri A (2009). "Sperm-associated antigen 9 is a biomarker for early cervical carcinoma". Cancer. 115 (12): 2671–83. doi: 10.1002/cncr.24293 . PMID   19326449. S2CID   23461660.
9. Kanojia D, Garg M, Saini S, Agarwal S, Parashar D, Jagadish N, Seth A, Bhatnagar A, Gupta A, Kumar R, Lohiya NK, Suri A (2013). "Sperm associated antigen 9 plays an important role in bladder transitional cell carcinoma". PLOS ONE. 8 (12) e81348. Bibcode:2013PLoSO...881348K. doi: 10.1371/journal.pone.0081348 . PMC   3857194 . PMID   24349057.
10. Ren B, Wei X, Zou G, He J, Xu G, Xu F, Huang Y, Zhu H, Li Y, Ma G, Yu P (2016). "Cancer testis antigen SPAG9 is a promising marker for the diagnosis and treatment of lung cancer". Oncology Reports. 35 (5): 2599–605. doi:10.3892/or.2016.4645. PMC   4811394 . PMID   26934841.
11. Lee CM, Onésime D, Reddy CD, Dhanasekaran N, Reddy EP (Oct 2002). "JLP: A scaffolding protein that tethers JNK/p38MAPK signaling modules and transcription factors". Proceedings of the National Academy of Sciences of the United States of America. 99 (22): 14189–94. Bibcode:2002PNAS...9914189L. doi: 10.1073/pnas.232310199 . PMC   137859 . PMID   12391307.

Further reading

• Ichijo H (Nov 1999). "From receptors to stress-activated MAP kinases". Oncogene. 18 (45): 6087–93. doi: 10.1038/sj.onc.1203129 . PMID   10557099.
• Nakajima D, Okazaki N, Yamakawa H, Kikuno R, Ohara O, Nagase T (Jun 2002). "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones". DNA Research. 9 (3): 99–106. doi: 10.1093/dnares/9.3.99 . PMID   12168954.
• Nagase T, Ishikawa K, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (Feb 1998). "Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Research. 5 (1): 31–9. doi: 10.1093/dnares/5.1.31 . PMID   9628581.
• Lee CM, Onésime D, Reddy CD, Dhanasekaran N, Reddy EP (Oct 2002). "JLP: A scaffolding protein that tethers JNK/p38MAPK signaling modules and transcription factors". Proceedings of the National Academy of Sciences of the United States of America. 99 (22): 14189–94. Bibcode:2002PNAS...9914189L. doi: 10.1073/pnas.232310199 . PMC   137859 . PMID   12391307.
• Yasuoka H, Ihn H, Medsger TA, Hirakata M, Kawakami Y, Ikeda Y, Kuwana M (Dec 2003). "A novel protein highly expressed in testis is overexpressed in systemic sclerosis fibroblasts and targeted by autoantibodies". Journal of Immunology. 171 (12): 6883–90. doi: 10.4049/jimmunol.171.12.6883 . PMID   14662895.
• Bouwmeester T, Bauch A, Ruffner H, Angrand PO, Bergamini G, Croughton K, Cruciat C, Eberhard D, Gagneur J, Ghidelli S, Hopf C, Huhse B, Mangano R, Michon AM, Schirle M, Schlegl J, Schwab M, Stein MA, Bauer A, Casari G, Drewes G, Gavin AC, Jackson DB, Joberty G, Neubauer G, Rick J, Kuster B, Superti-Furga G (Feb 2004). "A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway". Nature Cell Biology. 6 (2): 97–105. doi:10.1038/ncb1086. PMID   14743216. S2CID   11683986.
• Brill LM, Salomon AR, Ficarro SB, Mukherji M, Stettler-Gill M, Peters EC (May 2004). "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry". Analytical Chemistry. 76 (10): 2763–72. doi:10.1021/ac035352d. PMID   15144186.
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (Aug 2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the United States of America. 101 (33): 12130–5. Bibcode:2004PNAS..10112130B. doi: 10.1073/pnas.0404720101 . PMC   514446 . PMID   15302935.
• Jagadish N, Rana R, Selvi R, Mishra D, Garg M, Yadav S, Herr JC, Okumura K, Hasegawa A, Koyama K, Suri A (Jul 2005). "Characterization of a novel human sperm-associated antigen 9 (SPAG9) having structural homology with c-Jun N-terminal kinase-interacting protein". The Biochemical Journal. 389 (Pt 1): 73–82. doi:10.1042/BJ20041577. PMC   1184539 . PMID   15693750.
• Jagadish N, Rana R, Mishra D, Kumar M, Suri A (Jun 2005). "Sperm associated antigen 9 (SPAG9): a new member of c-Jun NH2 -terminal kinase (JNK) interacting protein exclusively expressed in testis". The Keio Journal of Medicine. 54 (2): 66–71. doi: 10.2302/kjm.54.66 . PMID   16077255.
• Guinn BA, Bland EA, Lodi U, Liggins AP, Tobal K, Petters S, Wells JW, Banham AH, Mufti GJ (Oct 2005). "Humoral detection of leukaemia-associated antigens in presentation acute myeloid leukaemia". Biochemical and Biophysical Research Communications. 335 (4): 1293–304. doi:10.1016/j.bbrc.2005.08.024. PMID   16112646.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID   16189514. S2CID   4427026.
• Rana R, Jagadish N, Garg M, Mishra D, Dahiya N, Chaurasiya D, Suri A (Feb 2006). "Small interference RNA-mediated knockdown of sperm associated antigen 9 having structural homology with c-Jun N-terminal kinase-interacting protein". Biochemical and Biophysical Research Communications. 340 (1): 158–64. doi:10.1016/j.bbrc.2005.11.168. PMC   7092841 . PMID   16356479.
• Rana R, Jagadish N, Garg M, Mishra D, Dahiya N, Chaurasiya D, Suri A (Nov 2006). "Immunogenicity study of recombinant human sperm-associated antigen 9 in bonnet macaque (Macaca radiata)". Human Reproduction. 21 (11): 2894–900. doi: 10.1093/humrep/del068 . PMID   16959808.