TASP1

TASP1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2A8I, 2A8J, 2A8L, 2A8M
Identifiers
Aliases	TASP1 , C20orf13, dJ585I14.2, taspase 1, SULEHS
External IDs	OMIM: 608270; MGI: 1923062; HomoloGene: 9795; GeneCards: TASP1; OMA:TASP1 - orthologs
Gene location (Human) Chr. Chromosome 20 (human) [1] Band 20p12.1 Start 13,389,392 bp [1] End 13,638,932 bp [1]
Gene location (Mouse) Chr. Chromosome 2 (mouse) [2] Band 2|2 F3 Start 139,675,400 bp [2] End 139,908,725 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in popliteal artery tibial arteries testicle Achilles tendon secondary oocyte epithelium of nasopharynx Descending thoracic aorta tibia ascending aorta bronchial epithelial cell Top expressed in spermatid Region I of hippocampus proper pineal gland barrel cortex spermatocyte lacrimal gland dentate gyrus of hippocampal formation granule cell lens right kidney proximal tubule More reference expression data BioGPS More reference expression data
Gene ontology Molecular function peptidase activity hydrolase activity threonine-type endopeptidase activity identical protein binding Cellular component cytoplasm Biological process positive regulation of transcription, DNA-templated proteolysis protein maturation Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 55617 75812 Ensembl ENSG00000089123 ENSMUSG00000039033 UniProt Q9H6P5 Q8R1G1 RefSeq (mRNA) NM_017714 NM_001323602 NM_001323603 NM_001323604 NM_001159640 NM_001159641 NM_001289611 NM_175225 RefSeq (protein) NP_001310531 NP_001310532 NP_001310533 NP_060184 NP_001153112 NP_001153113 NP_001276540 NP_780434 Location (UCSC) Chr 20: 13.39 – 13.64 Mb Chr 2: 139.68 – 139.91 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Threonine aspartase 1 is an enzyme that in humans is encoded by the TASP1 gene. ^{[5] [6]}

Function

This gene encodes an endopeptidase that cleaves specific substrates following aspartate residues. The encoded protein undergoes posttranslational autoproteolytic processing to generate alpha and beta subunits, which reassemble into the active alpha2-beta2 heterotetramer. It is required to cleave MLL, a protein required for the maintenance of HOX gene expression, and TFIIA, a basal transcription factor. Cleavage of TFIIA has been found to drive spermatogenesis. ^[7]

Alternatively spliced transcript variants have been described, but their biological validity has not been determined. ^[6]

Clinical significance

Taspase1 is overexpressed in primary human cancers and functions as a non-oncogene addiction protease that coordinates cancer cell proliferation and apoptosis. Therefore, Taspase1 may serve as a novel anti-cancer therapeutic target. ^[8]

References

1. GRCh38: Ensembl release 89: ENSG00000089123 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000039033 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Hsieh JJ, Cheng EH, Korsmeyer SJ (Nov 2003). "Taspase1: a threonine aspartase required for cleavage of MLL and proper HOX gene expression". Cell. 115 (3): 293–303. doi: 10.1016/S0092-8674(03)00816-X . PMID   14636557. S2CID   18952302.
6. "Entrez Gene: TASP1 taspase, threonine aspartase, 1".
7. Oyama, T; Sasagawa, S; Takeda, S; Hess, R. A.; Lieberman, P. M.; Cheng, E. H.; Hsieh, J. J. (2013). "Cleavage of TFIIA by Taspase1 activates TRF2-specified mammalian male germ cell programs". Developmental Cell. 27 (2): 188–200. doi:10.1016/j.devcel.2013.09.025. PMC   3947863 . PMID   24176642.
8. Chen DY, Liu H, Takeda S, Tu HC, Sasagawa S, Van Tine BA, Lu D, Cheng EH, Hsieh JJ (July 2010). "Taspase1 functions as a non-oncogene addiction protease that coordinates cancer cell proliferation and apoptosis". Cancer Res. 70 (13): 5358–67. doi:10.1158/0008-5472.CAN-10-0027. PMC   2909739 . PMID   20516119.

Further reading

• Zhou H, Spicuglia S, Hsieh JJ, et al. (2006). "Uncleaved TFIIA is a substrate for taspase 1 and active in transcription". Mol. Cell. Biol. 26 (7): 2728–35. doi:10.1128/MCB.26.7.2728-2735.2006. PMC   1430320 . PMID   16537915.
• Khan JA, Dunn BM, Tong L (2006). "Crystal structure of human Taspase1, a crucial protease regulating the function of MLL". Structure. 13 (10): 1443–52. doi: 10.1016/j.str.2005.07.006 . PMID   16216576.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC   528928 . PMID   15489334.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi: 10.1038/ng1285 . PMID   14702039.
• Hsieh JJ, Ernst P, Erdjument-Bromage H, et al. (2003). "Proteolytic cleavage of MLL generates a complex of N- and C-terminal fragments that confers protein stability and subnuclear localization". Mol. Cell. Biol. 23 (1): 186–94. doi:10.1128/MCB.23.1.186-194.2003. PMC   140678 . PMID   12482972.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Deloukas P, Matthews LH, Ashurst J, et al. (2002). "The DNA sequence and comparative analysis of human chromosome 20". Nature. 414 (6866): 865–71. Bibcode:2001Natur.414..865D. doi: 10.1038/414865a . PMID   11780052.