TRIM45

TRIM45
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2DS4
Identifiers
Aliases	TRIM45 , RNF99, tripartite motif containing 45
External IDs	OMIM: 609318 MGI: 1918187 HomoloGene: 11865 GeneCards: TRIM45
Gene location (Human) Chr. Chromosome 1 (human) [1] Band 1p13.1 Start 117,111,060 bp [1] End 117,122,587 bp [1]
Gene location (Mouse) Chr. Chromosome 3 (mouse) [2] Band 3|3 F2.2 Start 100,829,518 bp [2] End 100,844,236 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in secondary oocyte right uterine tube gastrocnemius muscle tibialis anterior muscle deltoid muscle body of pancreas biceps brachii quadriceps femoris muscle vastus lateralis muscle thyroid gland Top expressed in otolith organ utricle olfactory epithelium seminiferous tubule internal carotid artery external carotid artery spermatid Jacobson's organ hand trigeminal ganglion More reference expression data BioGPS n/a
Gene ontology Molecular function zinc ion binding metal ion binding Cellular component intracellular anatomical structure nucleus nucleoplasm intercellular bridge cytoplasm cytosol Biological process bone development Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 80263 229644 Ensembl ENSG00000134253 ENSMUSG00000033233 UniProt Q9H8W5 Q6PFY8 RefSeq (mRNA) NM_001145635 NM_025188 NM_001165952 NM_001165953 NM_194343 RefSeq (protein) NP_001139107 NP_079464 NP_001159424 NP_001159425 NP_919324 Location (UCSC) Chr 1: 117.11 – 117.12 Mb Chr 3: 100.83 – 100.84 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

tripartite motif containing 45, also known as TRIM45, is a human gene. ^[5]

This gene encodes a member of the tripartite motif family. The encoded protein may function as a [transcriptional repressor of the mitogen-activated protein kinase pathway. Alternatively spliced transcript variants have been described. ^[5]

Model organisms

Trim45 knockout mouse phenotype

Characteristic	Phenotype
Homozygote viability	Abnormal
Recessive lethal study	Abnormal
Body weight	Normal
Anxiety	Normal
Neurological assessment	Normal
Grip strength	Normal
Hot plate	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Body temperature	Normal
Eye morphology	Normal
Clinical chemistry	Abnormal [6]
Plasma immunoglobulins	Normal
Haematology	Normal
Peripheral blood lymphocytes	Normal
Micronucleus test	Normal
Heart weight	Normal
Tail epidermis wholemount	Normal
Skin Histopathology	Normal
Brain histopathology	Normal
MicroCT & Quantitative Faxitron	Abnormal
Salmonella infection	Normal [7]
Citrobacter infection	Normal [8]
All tests and analysis from [9] [10]

Model organisms have been used in the study of TRIM45 function. A conditional knockout mouse line, called Trim45^{tm1a(KOMP)Wtsi [11] [12]} was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists. ^{[13] [14] [15]}

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion. ^{[9] [16]} Twenty six tests were carried out on mutant mice and three significant abnormalities were observed. ^[9] No homozygous mutant embryos were identified during gestation, and therefore none survived until weaning. The remaining tests were carried out on heterozygous mutant adult mice; males had increased circulating magnesium levels while animals of both sex displayed increased bone strength. ^[9]

References

1. GRCh38: Ensembl release 89: ENSG00000134253 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000033233 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: tripartite motif containing 45" . Retrieved 2011-08-30.
6. "Clinical chemistry data for Trim45". Wellcome Trust Sanger Institute.
7. "Salmonella infection data for Trim45". Wellcome Trust Sanger Institute.
8. "Citrobacter infection data for Trim45". Wellcome Trust Sanger Institute.
9. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. S2CID   85911512.
10. Mouse Resources Portal, Wellcome Trust Sanger Institute.
11. "International Knockout Mouse Consortium".
12. "Mouse Genome Informatics".
13. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC   3572410 . PMID   21677750.
14. Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi: 10.1038/474262a . PMID   21677718.
15. Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi: 10.1016/j.cell.2006.12.018 . PMID   17218247. S2CID   18872015.
16. van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biology. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC   3218837 . PMID   21722353.

Further reading

• Sardiello M, Cairo S, Fontanella B, Ballabio A, Meroni G (2008). "Genomic analysis of the TRIM family reveals two groups of genes with distinct evolutionary properties". BMC Evolutionary Biology. 8: 225. doi:10.1186/1471-2148-8-225. PMC   2533329 . PMID   18673550.
• Wang Y, Li Y, Qi X, Yuan W, Ai J, Zhu C, Cao L, Yang H, Liu F, Wu X, Liu M (Oct 2004). "TRIM45, a novel human RBCC/TRIM protein, inhibits transcriptional activities of ElK-1 and AP-1". Biochemical and Biophysical Research Communications. 323 (1): 9–16. doi:10.1016/j.bbrc.2004.08.048. PMID   15351693.
• Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID   8125298.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID   9373149.