The South Florida Railroad was a railroad from Sanford, Florida, to Tampa, Florida, becoming part of the Plant System in 1893 and the Atlantic Coast Line Railroad in 1902. It served as the southernmost segment of the Atlantic Coast Line's main line. The line remains in service today and is now part of the Central Florida Rail Corridor in the Orlando metro area. The rest of the line remains under the ownership of CSX Transportation as part of their A Line.
The Florida Central and Peninsular Railroad was the final name of a system of railroads throughout Florida, becoming part of the Seaboard Air Line Railway in 1900. The system, including some of the first railroads in Florida, stretched from Jacksonville west through Tallahassee and south to Tampa. Much of the FC&P network is still in service under the ownership of CSX Transportation.
The Tampa Southern Railroad was a subsidiary of the Atlantic Coast Line Railroad (ACL) originally running from Uceta Yard in Tampa south to Palmetto, Bradenton, and Sarasota with a later extension southeast to Fort Ogden in the Peace River valley built shortly after. It was one of many rail lines completed during the Florida land boom of the 1920s. Most of the remaining trackage now serves as CSX Transportation's Palmetto Subdivision. Another short portion just east of Sarasota also remains that is now operated by Seminole Gulf Railway.
The Florida Western and Northern Railroad was a subsidiary of the Seaboard Air Line Railroad that expanded their network in the 1920s by building a rail line from Coleman, Florida all the way to West Palm Beach via Auburndale and Sebring, a distance of 204 miles. The line would be extended to Miami by the Seaboard-All Florida Railway, another Seaboard Air Line subsidiary, shortly after with the full line from Coleman to Miami becoming the Seaboard Air Line's Miami Subdivision. The line is still in service today from Auburndale to West Palm Beach and is now operated by Seaboard successor CSX Transportation as their Auburndale Subdivision.
The Brewster Subdivision is a railroad line owned by CSX Transportation in Florida. The line runs from the Valrico Subdivision at Edison Junction south through Bradley Junction to Arcadia for a total of 47.2 miles. It junctions with the Achan Subdivision and the Agricola Spur at Bradley Junction, and connects to the Seminole Gulf Railway in Arcadia.
The Brooker Subdivision is a railroad line owned by CSX Transportation in Florida. The line runs from the CSX S Line at Wannee Junction in Starke to Newberry for a total of 39.6 miles. At its north end it continues south from the Wildwood Subdivision and at its south end it junctions at a wye with the Florida Northern Railroad.
CSX Transportation's Brooksville Subdivision is a railroad line in the Tampa Bay region of Florida. Today, the line runs from the Clearwater Subdivision in Sulphur Springs, Florida north to a point just north of Brooksville, a distance of 49.1 miles. Historically, the Brooksville Subdivision was owned and operated by CSX predecessor, the Seaboard Air Line Railroad. Under Seaboard ownership, the Brooksville Subdivision continued north as far as Waldo, where it intercepted the Seaboard Air Line main line.
CSX Transportation's Carters Subdivision is a segment of CSX's A Line running from Poinciana southwest to Lakeland. The Carters Subdivision ends just west of Downtown Lakeland at Lakeland Junction, where it continues west as the Lakeland Subdivision and connects to the Vitis Subdivision.
The Lakeland Subdivision is a CSX Transportation rail line in Florida. It runs along CSX's A Line from just west of Lakeland and heads west through Plant City to the community of Mango in Hillsborough County, just east of Tampa.
The Tampa Terminal Subdivision is a group of railroad lines owned by CSX Transportation in and around Tampa, Florida. The Tampa Terminal Subdivision covers track around Yeoman and Uceta Yards and is located at the end of two of CSX's U.S. East Coast main lines to Richmond, Virginia, the A Line and the S Line.
CSX Transportation's Valrico Subdivision is a railroad line in Central Florida. It serves as CSX's main route through a region of Central Florida known as the Bone Valley, which contains the largest known deposits of phosphate in the United States.
The Wildwood Subdivision is a railroad line owned by CSX Transportation in Florida. It runs along CSX's S Line from Baldwin south to Zephyrhills via Ocala and Wildwood for a total of 155.7 miles. The S Line is CSX's designation for the line that was the Seaboard Air Line Railroad main line from 1903 to 1967.
The Yeoman Subdivision is a railroad line owned by CSX Transportation in Florida. It runs along CSX’s S Line from Zephyrhills south to just east of Tampa via Plant City for a total of 31.2 miles.
The Winston and Bone Valley Railroad was a railroad line running the Bone Valley region of Central Florida. It connected to the South Florida Railroad main line near Lakeland. A vast majority of the line remains in service by CSX Transportation, who operates it today as their Bone Valley Subdivision.
The Atlantic Coast Line Railroad's Haines City Branch was a railroad line running from their main line in Haines City, Florida south through southern Central Florida. The line notably ran through Lake Wales, Avon Park, Sebring, and Immokalee and would stretch as far south as Everglades City upon its completion in 1928. Everglades City would be the southernmost point the entire Atlantic Coast Line Railroad system would ever reach. The Haines City Branch was one of the Atlantic Coast Line's major additions to its Florida network, much of which was previously part of the Plant System.
The Live Oak, Tampa and Charlotte Harbor Railroad was a historic railroad in Florida chartered by railroad tycoon Henry B. Plant. It was built as an extension of Plant's Live Oak and Rowlands Bluff Railroad. Together, the two lines ran from Live Oak, Florida to Gainesville via High Springs. The lines were completed in 1884.
The Atlantic Coast Line Railroad's Lakeland—Fort Myers Line was one of the railroad company's secondary main lines in Central and Southwest Florida. It was built incrementally in the late 1800s and early 1900s. Parts of the line are still active today.
The Seaboard Air Line Railroad's Sarasota Subdivision was a rail line that ran from the company's main line at Turkey Creek south to Palmetto, Bradenton, Sarasota, and Venice. The line was built in phases from 1901 to 1911.
The Atlantic Coast Line Railroad's DuPont—Lakeland Line was a historic rail line in southern Georgia and the northern west coast of Florida. On employee timetables, the line was actually divided into the DuPont—High Springs Line and the High Springs—Lakeland Line. The line was primarily used for freight, though some passenger services ran on parts of it in Florida. While parts of the line were built as early as 1863, the full line was not complete until 1913. Parts of the line in Florida are still active today.
The Seaboard Air Line Railroad’s Main Line was the backbone of the Seaboard Air Line Railroad's network in the southeastern United States. The main line ran from Richmond, Virginia to Tampa, Florida, a distance of over 800 miles. Along its route it passed through Petersburg, Raleigh, Columbia, Savannah, Jacksonville, and Ocala, Florida. While some segments of the line have been abandoned as of 2022, most of the line is still in service and is owned by the Seaboard Air Line's successor, CSX Transportation as their S Line.
