The Atlantic, Suwannee River and Gulf Railroad Company was a railroad that ran westward from Starke, Florida, eventually terminating at Wannee, Florida, on the Suwannee River. It was later absorbed by the Seaboard Air Line Railroad becoming their Wannee Subdivision.
The South Florida Railroad was a railroad from Sanford, Florida, to Tampa, Florida, becoming part of the Plant System in 1893 and the Atlantic Coast Line Railroad in 1902. It served as the southernmost segment of the Atlantic Coast Line's main line. The line remains in service today and is now part of the Central Florida Rail Corridor in the Orlando metro area. The rest of the line remains under the ownership of CSX Transportation as part of their A Line.
The Plant System, named after its owner, Henry B. Plant, was a system of railroads and steamboats in the U.S. South, taken over by the Atlantic Coast Line Railroad in 1902. The original line of the system was the Savannah, Florida and Western Railway, running across southern Georgia. The Plant Investment Company was formed in 1882 to lease and buy other railroads and expand the system. Other major lines incorporated into the system include the Savannah and Charleston Railroad and the Brunswick and Western Railroad.
The Riceboro Southern Railway began operations in 2004 operating on about 33 miles of track, some of which is leased from CSX Transportation. The track on which it operates is part of the ex-Seaboard Air Line route from Savannah, Georgia to Jacksonville, Florida. It runs generally from Ogeechee, Georgia, where the line splits from the CSX Savannah Subdivision, which is the former Atlantic Coast Line Railroad's Savannah-Jacksonville route, and Riceboro. It does not have any of its own locomotives; it uses Georgia Central power.
The First Coast Railroad is a class III railroad operating in Florida and Georgia, owned by Genesee & Wyoming. The name is derived from its area of operations around the First Coast of Florida.
The Ocala Union Station is a bus station and former train station in Ocala, Florida, United States. It is located at 531 Northeast First Avenue, and was built in 1917 by both the Atlantic Coast Line and Seaboard Air Line Railroad. Prior to this, ACL and SAL had separate depots in Ocala. The former ACL station was originally built by the Florida Southern Railroad, while the former SAL station was built by the Florida Transit and Peninsular Railroad. On December 22, 1997, it was added to the U.S. National Register of Historic Places.
The Florida Railroad was the first railroad to connect the east and west coasts of Florida, running from Fernandina to Cedar Key. The line later became part of the Seaboard Air Line Railroad, and, where still in use, is operated by CSX Transportation and the First Coast Railroad. The highway corridor of SR 24, US 301, and SR A1A/SR 200 closely parallels the former Florida Railroad.
The Inland Lakes Railway, later known as Florida Rail Adventures, was a tourist railroad located in the northwestern portion of Central Florida. The railway operated several excursion trains ranging from a five course dinner train to sightseeing excursions throughout Lake and Orange Counties. The company operated through a lease agreement with the Florida Central Railroad, the owner of the tracks. Trains operated between the towns of Mount Dora, Tavares, Eustis, Lake Jem, and Orlando.
The Jacksonville & Southwestern Railroad (J&SW) was a railroad that served Florida from 1899 to 1904. It was purchased by the Atlantic Coast Line Railroad in 1904. The Atlantic Coast Line would extend the line further west and it would become their Jacksonville—Wilcox Line. Some of the original right-of-way was converted to a recreational path in the rails to trails program in the 1990s.
The Callahan Subdivision is a CSX Transportation railroad subdivision within the Jacksonville Division on the former Seaboard Air Line Railroad. The sub extends northward 20 miles from Baldwin, Florida, where the Jacksonville Terminal Subdivision's S Line and Florida Gulf & Atlantic Railroad meet just north of Baldwin Yard, a classification yard. According to Jacksonville Division Timetable Number 4 published in 2005, the sub runs from milepost SM 0.18 to milepost SM 20.0, where it joins the Nahunta Subdivision, a former Atlantic Coast Line Railroad, in Callahan, Florida. The line serves as a bypass to Jacksonville.
The Brooker Subdivision is a railroad line owned by CSX Transportation in Florida. The line runs from the CSX S Line at Wannee Junction in Starke to Newberry for a total of 39.6 miles. At its north end it continues south from the Wildwood Subdivision and at its south end it junctions at a wye with the Florida Northern Railroad.
CSX Transportation's Brooksville Subdivision is a railroad line in the Tampa Bay region of Florida. Today, the line runs from the Clearwater Subdivision in Sulphur Springs, Florida north to a point just north of Brooksville, a distance of 49.1 miles. Historically, the Brooksville Subdivision was owned and operated by CSX predecessor, the Seaboard Air Line Railroad. Under Seaboard ownership, the Brooksville Subdivision continued north as far as Waldo, where it intercepted the Seaboard Air Line main line.
The Kingsland Subdivision is a railroad line owned by CSX Transportation in Northeast Florida. The line begins in Jacksonville at a junction with the A Line near Moncrief Yard. From there, it heads east and then turns north near Panama Park. From Panama Park, the Kingsland Subdivision heads north to Yulee on a discontinuous piece of CSX's S Line. In Yulee, the line connects with the First Coast Railroad, which operates north to Seals, Georgia, on tracks that were previously part of the Kingsland Subdivision prior to 2005. The line's namesake is Kingsland, Georgia, which is located north of Yulee on the First Coast Railroad.
The Wildwood Subdivision is a railroad line owned by CSX Transportation in Florida. It runs along CSX's S Line from Baldwin south to Zephyrhills via Ocala and Wildwood for a total of 155.7 miles. The S Line is CSX's designation for the line that was the Seaboard Air Line Railroad main line from 1903 to 1967.
The Yeoman Subdivision is a railroad line owned by CSX Transportation in Florida. It runs along CSX’s S Line from Zephyrhills south to just east of Tampa via Plant City for a total of 31.2 miles.
The Florida Gulf and Atlantic Railroad is a shortline railroad owned and operated by RailUSA in the Florida Panhandle. The line consists of 430 miles of track: a main line from Baldwin, Florida, through Tallahassee to Pensacola, as well as a branch from Tallahassee north to Attapulgus, Georgia.
The Live Oak, Tampa and Charlotte Harbor Railroad was a historic railroad in Florida chartered by railroad tycoon Henry B. Plant. It was built as an extension of Plant's Live Oak and Rowlands Bluff Railroad. Together, the two lines ran from Live Oak, Florida to Gainesville via High Springs. The lines were completed in 1884.
The CSX A Line forms the backbone of the historic Atlantic Coast Line Railroad Main Line, the backbone of their network in the southeastern United States. The main line runs from Richmond, Virginia to Port Tampa just southwest of Tampa, Florida, a distance of nearly 900 miles. Along its route it passes through Petersburg, Rocky Mount, Florence, Charleston, Savannah, Jacksonville, and Orlando. With the exception of a short 61-mile segment in Greater Orlando, the entire line is owned by CSX Transportation.
The Seaboard Air Line Railroad’s Main Line was the backbone of the Seaboard Air Line Railroad's network in the southeastern United States. The main line ran from Richmond, Virginia to Tampa, Florida, a distance of over 800 miles. Along its route it passed through Petersburg, Raleigh, Columbia, Savannah, Jacksonville, and Ocala, Florida. While some segments of the line have been abandoned as of 2023, most of the line is still in service and is owned by the Seaboard Air Line's successor, CSX Transportation as their S-Line.
