The Florida Northern Railroad Company, Inc. is one of several short line railroads run by Regional Rail, LLC. It has connections to CSX at Ocala, Florida, running north to Lowell, Florida, and south to Candler, Florida. It was formerly run by CSX as their Ocala Subdivision.
The Florida Central and Peninsular Railroad was the final name of a system of railroads throughout Florida, becoming part of the Seaboard Air Line Railway in 1900. The system, including some of the first railroads in Florida, stretched from Jacksonville west through Tallahassee and south to Tampa. Much of the FC&P network is still in service under the ownership of CSX Transportation.
The Riceboro Southern Railway began operations in 2004 operating on about 33 miles of track, some of which is leased from CSX Transportation. The track on which it operates is part of the ex-Seaboard Air Line route from Savannah, Georgia to Jacksonville, Florida. It runs generally from Ogeechee, Georgia, where the line splits from the CSX Savannah Subdivision, which is the former Atlantic Coast Line Railroad's Savannah-Jacksonville route, and Riceboro. It does not have any of its own locomotives; it uses Georgia Central power.
The First Coast Railroad is a class III railroad operating in Florida and Georgia, owned by Genesee & Wyoming. The name is derived from its area of operations around the First Coast of Florida.
The Jacksonville & Southwestern Railroad (J&SW) was a railroad that served Florida from 1899 to 1904. It was purchased by the Atlantic Coast Line Railroad in 1904. The Atlantic Coast Line would extend the line further west and it would become their Jacksonville—Wilcox Line. Some of the original right-of-way was converted to a recreational path in the rails to trails program in the 1990s.
The Callahan Subdivision is a CSX Transportation railroad subdivision within the Jacksonville Division on the former Seaboard Air Line Railroad. The sub extends northward 20 miles from Baldwin, Florida, where the Jacksonville Terminal Subdivision's S Line and Florida Gulf & Atlantic Railroad meet just north of Baldwin Yard, a classification yard. According to Jacksonville Division Timetable Number 4 published in 2005, the sub runs from milepost SM 0.18 to milepost SM 20.0, where it joins the Nahunta Subdivision, a former Atlantic Coast Line Railroad, in Callahan, Florida. The line serves as a bypass to Jacksonville.
The Brooker Subdivision is a railroad line owned by CSX Transportation in Florida. The line runs from the CSX S Line at Wannee Junction in Starke to Newberry for a total of 39.6 miles. At its north end it continues south from the Wildwood Subdivision and at its south end it junctions at a wye with the Florida Northern Railroad.
CSX Transportation's Brooksville Subdivision is a railroad line in the Tampa Bay region of Florida. Today, the line runs from the Clearwater Subdivision in Sulphur Springs, Florida north to a point just north of Brooksville, a distance of 49.1 miles. Historically, the Brooksville Subdivision was owned and operated by CSX predecessor, the Seaboard Air Line Railroad. Under Seaboard ownership, the Brooksville Subdivision continued north as far as Waldo, where it intercepted the Seaboard Air Line main line.
The Jacksonville Terminal Subdivision is a group of railroad lines owned by CSX Transportation in and around Jacksonville, which was historically a major railroad hub. The Jacksonville Terminal Subdivision includes about 13.0 miles of track.
The Kingsland Subdivision is a railroad line owned by CSX Transportation in Northeast Florida. The line begins in Jacksonville at a junction with the A Line near Moncrief Yard. From there, it heads east and then turns north near Panama Park. From Panama Park, the Kingsland Subdivision heads north to Yulee on a discontinuous piece of CSX's S Line. In Yulee, the line connects with the First Coast Railroad, which operates north to Seals, Georgia, on tracks that were previously part of the Kingsland Subdivision prior to 2005. The line's namesake is Kingsland, Georgia, which is located north of Yulee on the First Coast Railroad.
The Wildwood Subdivision is a railroad line owned by CSX Transportation in Florida. It runs along CSX's S Line from Baldwin south to Zephyrhills via Ocala and Wildwood for a total of 155.7 miles. The S Line is CSX's designation for the line that was the Seaboard Air Line Railroad main line from 1903 to 1967.
The Tampa Northern Railroad was a historic railroad line running from just east of downtown Tampa north to the city of Brooksville in Hernando County. The line continues to operate today and is under the ownership of the CSX Corporation, who operates it as their Brooksville Subdivision north of Sulphur Springs and part of their Clearwater Subdivision south of there.
The Plant City, Arcadia, and Gulf Railroad was a railroad line that once operated from Plant City, Florida, south to Welcome, a distance of about 13 miles. The line remains in service today and is owned by CSX Transportation, which it operates as their Plant City Subdivision.
The Live Oak, Tampa and Charlotte Harbor Railroad was a historic railroad in Florida chartered by railroad tycoon Henry B. Plant. It was built as an extension of Plant's Live Oak and Rowlands Bluff Railroad. Together, the two lines ran from Live Oak, Florida to Gainesville via High Springs. The lines were completed in 1884.
The Gross Cutoff was a rail line built by the Seaboard Air Line Railroad in northern Florida. It ran from the Seaboard Air Line Railroad’s main line at a point known as Gross to Callahan connecting two pre-existing tracks.
The CSX A Line forms the backbone of the historic Atlantic Coast Line Railroad Main Line, the backbone of their network in the southeastern United States. The main line runs from Richmond, Virginia to Port Tampa just southwest of Tampa, Florida, a distance of nearly 900 miles. Along its route it passes through Petersburg, Rocky Mount, Florence, Charleston, Savannah, Jacksonville, and Orlando. With the exception of a short 61-mile segment in Greater Orlando, the entire line is owned by CSX Transportation.
The Seaboard Air Line Railroad's Sarasota Subdivision was a rail line that ran from the company's main line at Turkey Creek south to Palmetto, Bradenton, Sarasota, and Venice. The line was built in phases from 1901 to 1911.
The Atlantic Coast Line Railroad's DuPont—Lakeland Line was a historic rail line in southern Georgia and the northern west coast of Florida. On employee timetables, the line was actually divided into the DuPont—High Springs Line and the High Springs—Lakeland Line. The line was primarily used for freight, though some passenger services ran on parts of it in Florida. While parts of the line were built as early as 1863, the full line was not complete until 1913. Parts of the line in Florida are still active today.
The Seaboard Air Line Railroad’s Main Line was the backbone of the Seaboard Air Line Railroad's network in the southeastern United States. The main line ran from Richmond, Virginia to Tampa, Florida, a distance of over 800 miles. Along its route it passed through Petersburg, Raleigh, Columbia, Savannah, Jacksonville, and Ocala, Florida. While some segments of the line have been abandoned as of 2023, most of the line is still in service and is owned by the Seaboard Air Line's successor, CSX Transportation as their S-Line.
