The Florida Midland Railroad Company, Inc. is one of several short line railroads operated by Regional Rail, LLC in Florida. The Florida Midland Railroad operates two former CSX Transportation railroad lines including their former Lake Wales Subdivision, and their former Bartow Subdivision. It once had a third line from Wildwood to Leesburg that is now mostly abandoned. Florida Midland Railroad began operating the lines in 1987 and uses locomotives branded for the Florida Central Railroad, its sister railroad.
The Florida Central and Peninsular Railroad was the final name of a system of railroads throughout Florida, becoming part of the Seaboard Air Line Railway in 1900. The system, including some of the first railroads in Florida, stretched from Jacksonville west through Tallahassee and south to Tampa. Much of the FC&P network is still in service under the ownership of CSX Transportation.
The Riceboro Southern Railway began operations in 2004 operating on about 33 miles of track, some of which is leased from CSX Transportation. The track on which it operates is part of the ex-Seaboard Air Line route from Savannah, Georgia to Jacksonville, Florida. It runs generally from Ogeechee, Georgia, where the line splits from the CSX Savannah Subdivision, which is the former Atlantic Coast Line Railroad's Savannah-Jacksonville route, and Riceboro. It does not have any of its own locomotives; it uses Georgia Central power.
The First Coast Railroad is a class III railroad operating in Florida and Georgia, owned by Genesee & Wyoming. The name is derived from its area of operations around the First Coast of Florida.
The Florida Railroad was the first railroad to connect the east and west coasts of Florida, running from Fernandina to Cedar Key. The line later became part of the Seaboard Air Line Railroad, and, where still in use, is operated by CSX Transportation and the First Coast Railroad. The highway corridor of SR 24, US 301, and SR A1A/SR 200 closely parallels the former Florida Railroad.
The Callahan Subdivision is a CSX Transportation railroad subdivision within the Jacksonville Division on the former Seaboard Air Line Railroad. The sub extends northward 20 miles from Baldwin, Florida, where the Jacksonville Terminal Subdivision's S Line and Florida Gulf & Atlantic Railroad meet just north of Baldwin Yard, a classification yard. According to Jacksonville Division Timetable Number 4 published in 2005, the sub runs from milepost SM 0.18 to milepost SM 20.0, where it joins the Nahunta Subdivision, a former Atlantic Coast Line Railroad, in Callahan, Florida. The line serves as a bypass to Jacksonville.
The Brooker Subdivision is a railroad line owned by CSX Transportation in Florida. The line runs from the CSX S Line at Wannee Junction in Starke to Newberry for a total of 39.6 miles. At its north end it continues south from the Wildwood Subdivision and at its south end it junctions at a wye with the Florida Northern Railroad.
The Jacksonville Terminal Subdivision is a group of railroad lines owned by CSX Transportation in and around Jacksonville, which was historically a major railroad hub. The Jacksonville Terminal Subdivision includes about 13.0 miles of track.
The Kingsland Subdivision is a railroad line owned by CSX Transportation in Northeast Florida. The line begins in Jacksonville at a junction with the A Line near Moncrief Yard. From there, it heads east and then turns north near Panama Park. From Panama Park, the Kingsland Subdivision heads north to Yulee on a discontinuous piece of CSX's S Line. In Yulee, the line connects with the First Coast Railroad, which operates north to Seals, Georgia, on tracks that were previously part of the Kingsland Subdivision prior to 2005. The line's namesake is Kingsland, Georgia, which is located north of Yulee on the First Coast Railroad.
The Nahunta Subdivision is a railroad line owned by CSX Transportation in Florida and Georgia. The line runs along CSX's A Line from Ogeechee, Georgia, to Dinsmore, Florida, for a total of 129.2 miles (207.9 km). At its north end it continues south from the Savannah Subdivision and at its south end it continues south as the Jacksonville Terminal Subdivision A-Line. The line is double-tracked from Folkston, Georgia south. All of Amtrak's Florida service also traverses the line.
The Savannah Subdivision is a railroad line owned by CSX Transportation in the U.S. state of Georgia. Through the middle of Savannah, the Savannah Subdivision splits into an east route and a west route. The East Route runs from Savannah, Georgia, to Ogeechee, Georgia, for a total of 15.6 miles (25.1 km). At its north end it continues south from the Charleston Subdivision and the Columbia Subdivision both of the Florence Division and at its south end it continues south as the Nahunta Subdivision. The West Route is located in Savannah, Georgia, and is 9.7 miles (15.6 km) in length. At its north end it branches off of the Savannah Subdivision East Route and at its south end it comes back into the Savannah Subdivision East Route.
CSX Transportation's Valrico Subdivision is a railroad line in Central Florida. It serves as CSX's main route through a region of Central Florida known as the Bone Valley, which contains the largest known deposits of phosphate in the United States.
The Wildwood Subdivision is a railroad line owned by CSX Transportation in Florida. It runs along CSX's S Line from Baldwin south to Zephyrhills via Ocala and Wildwood for a total of 155.7 miles. The S Line is CSX's designation for the line that was the Seaboard Air Line Railroad main line from 1903 to 1967.
The PD Subdivision is a railroad line owned by CSX Transportation in Florida and Alabama. The line runs from Pensacola, Florida, north to Flomaton, Alabama, for a total of 41.0 miles (66.0 km). At its south end it connects to the Florida Gulf & Atlantic Railroad and at its north end it continues north as the M&M Subdivision.
The Charleston Subdivision is a railroad territory owned by CSX Transportation in the U.S. states of South Carolina and Georgia. The line from Florence, South Carolina, to Savannah, Georgia, for a total of 195.8 miles. At its north end it continues south from the South End Subdivision and at its south end it continues south as the Savannah Subdivision of the Jacksonville Division.
The Plant City, Arcadia, and Gulf Railroad was a railroad line that once operated from Plant City, Florida, south to Welcome, a distance of about 13 miles. The line remains in service today and is owned by CSX Transportation, which it operates as their Plant City Subdivision.
The South End Subdivision is a railroad line owned by CSX Transportation in the U.S. states of North Carolina and South Carolina. The line runs from Rocky Mount, North Carolina, to Florence, South Carolina, for a total of 172.8 miles (278.1 km). At its north end the line continues south from the North End Subdivision and at its south end the line continues south as the Charleston Subdivision. The South End Subdivision is a part of CSX's A Line, one of their mainline which ultimately extends from Richmond, Virginia to Tampa, Florida.
The Winston and Bone Valley Railroad was a railroad line running the Bone Valley region of Central Florida. It connected to the South Florida Railroad main line near Lakeland. A vast majority of the line remains in service by CSX Transportation, who operates it today as their Bone Valley Subdivision.
The Seaboard Air Line Railroad's Sarasota Subdivision was a rail line that ran from the company's main line at Turkey Creek south to Palmetto, Bradenton, Sarasota, and Venice. The line was built in phases from 1901 to 1911.
The Seaboard Air Line Railroad’s Main Line was the backbone of the Seaboard Air Line Railroad's network in the southeastern United States. The main line ran from Richmond, Virginia to Tampa, Florida, a distance of over 800 miles. Along its route it passed through Petersburg, Raleigh, Columbia, Savannah, Jacksonville, and Ocala, Florida. While some segments of the line have been abandoned as of 2023, most of the line is still in service and is owned by the Seaboard Air Line's successor, CSX Transportation as their S-Line.
