Transcription factor IIIB 50 kDa subunit

BRF2
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4ROD, 4ROE, 4ROC
Identifiers
Aliases	BRF2 , BRFU, TFIIIB50, RNA polymerase III transcription initiation factor 50 kDa subunit, RNA polymerase III transcription initiation factor subunit, BRF2 RNA polymerase III transcription initiation factor subunit
External IDs	OMIM: 607013; MGI: 1913903; HomoloGene: 10127; GeneCards: BRF2; OMA:BRF2 - orthologs
Gene location (Human) Chr. Chromosome 8 (human) [1] Band 8p11.23 Start 37,843,268 bp [1] End 37,849,861 bp [1]
Gene location (Mouse) Chr. Chromosome 8 (mouse) [2] Band 8|8 A2 Start 27,613,392 bp [2] End 27,618,708 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in Skeletal muscle tissue of rectus abdominis tibialis anterior muscle deltoid muscle Skeletal muscle tissue of biceps brachii gastrocnemius muscle quadriceps femoris muscle vastus lateralis muscle decidua beta cell muscle of thigh Top expressed in primary oocyte spermatid spermatocyte zygote secondary oocyte interventricular septum seminiferous tubule morula hand superior cervical ganglion More reference expression data BioGPS More reference expression data
Gene ontology Molecular function metal ion binding RNA polymerase III type 3 promoter sequence-specific DNA binding transcription factor binding Cellular component nucleus nucleoplasm transcription factor TFIIIB complex Biological process regulation of transcription, DNA-templated transcription, DNA-templated regulation of transcription by RNA polymerase III cellular response to oxidative stress transcription preinitiation complex assembly DNA-templated transcription, initiation RNA polymerase III preinitiation complex assembly Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 55290 66653 Ensembl ENSG00000104221 ENSMUSG00000031487 UniProt Q9HAW0 Q3UAW9 RefSeq (mRNA) NM_018310 NM_025686 RefSeq (protein) NP_060780 NP_079962 Location (UCSC) Chr 8: 37.84 – 37.85 Mb Chr 8: 27.61 – 27.62 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Transcription factor IIIB 50 kDa subunit (TFIIIB50) also known as b-related factor 2 (BRF-2) is a protein that in humans is encoded by the BRF2 gene. ^{[5] [6] [7]}

Function

This gene encodes one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. The product of this gene, a TFIIB-like factor, is directly recruited to the TATA box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein. ^[7]

References

1. GRCh38: Ensembl release 89: ENSG00000104221 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000031487 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Yu Y, Zhang C, Zhou G, Wu S, Qu X, Wei H, Xing G, Dong C, Zhai Y, Wan J, Ouyang S, Li L, Zhang S, Zhou K, Zhang Y, Wu C, He F (Aug 2001). "Gene expression profiling in human fetal liver and identification of tissue- and developmental-stage-specific genes through compiled expression profiles and efficient cloning of full-length cDNAs". Genome Res. 11 (8): 1392–403. doi:10.1101/gr.175501. PMC   311073 . PMID   11483580.
6. Cabart P, Murphy S (Nov 2001). "BRFU, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with TATA-binding protein". J Biol Chem. 276 (46): 43056–64. doi: 10.1074/jbc.M108515200 . PMID   11564744.
7. "Entrez Gene: BRF2 BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like".

Further reading

• Sutcliffe JE, Cairns CA, McLees A, et al. (1999). "RNA polymerase III transcription factor IIIB is a target for repression by pocket proteins p107 and p130". Mol. Cell. Biol. 19 (6): 4255–61. doi:10.1128/mcb.19.6.4255. PMC   104385 . PMID   10330166.
• Schramm L, Pendergrast PS, Sun Y, Hernandez N (2000). "Different human TFIIIB activities direct RNA polymerase III transcription from TATA-containing and TATA-less promoters". Genes Dev. 14 (20): 2650–63. doi:10.1101/gad.836400. PMC   316990 . PMID   11040218.
• Teichmann M, Wang Z, Roeder RG (2001). "A stable complex of a novel transcription factor IIB- related factor, human TFIIIB50, and associated proteins mediate selective transcription by RNA polymerase III of genes with upstream promoter elements". Proc. Natl. Acad. Sci. U.S.A. 97 (26): 14200–5. doi: 10.1073/pnas.97.26.14200 . PMC   18895 . PMID   11121026.
• Hinkley CS, Hirsch HA, Gu L, et al. (2003). "The small nuclear RNA-activating protein 190 Myb DNA binding domain stimulates TATA box-binding protein-TATA box recognition". J. Biol. Chem. 278 (20): 18649–57. doi: 10.1074/jbc.M204247200 . PMID   12621023.
• Saxena A, Ma B, Schramm L, Hernandez N (2005). "Structure-function analysis of the human TFIIB-related factor II protein reveals an essential role for the C-terminal domain in RNA polymerase III transcription". Mol. Cell. Biol. 25 (21): 9406–18. doi:10.1128/MCB.25.21.9406-9418.2005. PMC   1265830 . PMID   16227591.

External links

• FactorBook BRF2