USP11

USP11
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4MEL
Identifiers
Aliases	USP11 , UHX1, ubiquitin specific peptidase 11
External IDs	OMIM: 300050 MGI: 2384312 HomoloGene: 31252 GeneCards: USP11
Gene location (Human) Chr. X chromosome (human) [1] Band Xp11.3 Start 47,232,866 bp [1] End 47,248,328 bp [1]
Gene location (Mouse) Chr. X chromosome (mouse) [2] Band X|X A1.3 Start 20,570,145 bp [2] End 20,586,778 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in middle temporal gyrus pituitary gland anterior pituitary entorhinal cortex postcentral gyrus superior frontal gyrus nucleus accumbens dorsolateral prefrontal cortex cingulate gyrus hypothalamus Top expressed in dorsomedial hypothalamic nucleus arcuate nucleus paraventricular nucleus of hypothalamus median eminence ventromedial nucleus supraoptic nucleus lateral hypothalamus habenula ventral tegmental area subiculum More reference expression data BioGPS n/a
Gene ontology Molecular function peptidase activity cysteine-type endopeptidase activity cysteine-type peptidase activity protein binding hydrolase activity thiol-dependent deubiquitinase Cellular component cytoplasm nucleus nucleoplasm cytosol chromosome Biological process ubiquitin-dependent protein catabolic process proteolysis protein deubiquitination Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 8237 236733 Ensembl ENSG00000102226 ENSMUSG00000031066 UniProt P51784 Q99K46 RefSeq (mRNA) NM_004651 NM_001371072 NM_145628 NM_001358931 RefSeq (protein) NP_001358001 NP_663603 NP_001345860 Location (UCSC) Chr X: 47.23 – 47.25 Mb Chr X: 20.57 – 20.59 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Ubiquitin carboxyl-terminal hydrolase or Ubiquitin specific protease 11 is an enzyme that in humans is encoded by the USP11 gene. ^{[5] [6]} USP11 belongs to the Ubiquitin specific proteases family (USPs) which is a sub-family of the Deubiquitinating enzymes (DUBs).USPs are multiple domain proteases and belong to the C19 cysteine proteases sub‒family. Depending on their domain architecture and position there is different homology between the various members. Generally the largest domain is the catalytic domain which harbours the three residue catalytic triad that is included inside conserved motifs (Cys and His boxes). The catalytic domain also contains sequences that are not related with the catalysis function and their role is mostly not clearly understood at present, the length of these sequences varies for each USP and therefore the length of the whole catalytic domain can range from approximately 295 to 850 amino acids. ^[7] Particular sequences inside the catalytic domain or at the N‒terminus of some USPs have been characterised as UBL (Ubiquitin like) and DUSP (domain present in ubiquitin‒specific proteases) domains respectively. In some cases, regarding the UBL domains, it has been reported to have a catalysis enhancing function as in the case of USP7. ^[8] In addition, a so‒called DU domain module is the combination of a DUSP domain followed by a UBL domain separated by a linker and is found in USP11 as well as in USP15 and USP4.

USP11 is 963aa protein with a MW of approximately 109.8 kDa and a pI of ~5.28; it shares significant homology with USP15 and along with USP4 forms the DU subfamily. Nevertheless, alignment of the three USPs confirms that USP15 and USP4 are the closest homologues with the identity reaching ~73 % between their UBL1 domains whereas USP11 is the most distant member with an identity of only ~32.3 % when compared to USP15. An UBL2 domain insertion (285aa) is present within the catalytic domain, which encompasses amino acids 310‒931, and the catalytic triad consists of a cysteine, a histidine and an aspartic acid.

Function

Protein ubiquitination controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. This dynamic process, involving ubiquitin conjugating enzymes and deubiquitinating enzymes, adds and removes ubiquitin. Deubiquitinating enzymes are cysteine proteases that specifically cleave ubiquitin from ubiquitin-conjugated protein substrates. This gene encodes a deubiquitinating enzyme which lies in a gene cluster on chromosome Xp11.23 ^[6]

Interactions

USP11 has been shown to interact with RANBP9. ^[9]

References

1. GRCh38: Ensembl release 89: ENSG00000102226 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000031066 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Puente XS, Sánchez LM, Overall CM, López-Otín C (July 2003). "Human and mouse proteases: a comparative genomic approach". Nature Reviews. Genetics. 4 (7): 544–58. doi:10.1038/nrg1111. PMID   12838346. S2CID   2856065.
6. "Entrez Gene: USP11 ubiquitin specific peptidase 11".
7. Nijman SM, Luna-Vargas MP, Velds A, Brummelkamp TR, Dirac AM, Sixma TK, Bernards R (December 2005). "A genomic and functional inventory of deubiquitinating enzymes". Cell. 123 (5): 773–86. doi:10.1016/j.cell.2005.11.007. hdl: 1874/20959 . PMID   16325574. S2CID   15575576.
8. Faesen AC, Dirac AM, Shanmugham A, Ovaa H, Perrakis A, Sixma TK (October 2011). "Mechanism of USP7/HAUSP activation by its C-terminal ubiquitin-like domain and allosteric regulation by GMP-synthetase". Molecular Cell. 44 (1): 147–59. doi: 10.1016/j.molcel.2011.06.034 . hdl: 11858/00-001M-0000-002D-C745-C . PMID   21981925.
9. Ideguchi H, Ueda A, Tanaka M, Yang J, Tsuji T, Ohno S, Hagiwara E, Aoki A, Ishigatsubo Y (October 2002). "Structural and functional characterization of the USP11 deubiquitinating enzyme, which interacts with the RanGTP-associated protein RanBPM". The Biochemical Journal. 367 (Pt 1): 87–95. doi:10.1042/BJ20011851. PMC   1222860 . PMID   12084015.

Further reading

• D'Andrea A, Pellman D (1999). "Deubiquitinating enzymes: a new class of biological regulators". Critical Reviews in Biochemistry and Molecular Biology. 33 (5): 337–52. doi:10.1080/10409239891204251. PMID   9827704.
• Swanson DA, Freund CL, Ploder L, McInnes RR, Valle D (April 1996). "A ubiquitin C-terminal hydrolase gene on the proximal short arm of the X chromosome: implications for X-linked retinal disorders". Human Molecular Genetics. 5 (4): 533–8. doi: 10.1093/hmg/5.4.533 . PMID   8845848.
• Brandau O, Nyakatura G, Jedele KB, Platzer M, Achatz H, Ross M, Murken J, Rosenthal A, Meindl A (1999). "UHX1 and PCTK1: precise characterisation and localisation within a gene-rich region in Xp11.23 and evaluation as candidate genes for retinal diseases mapped to Xp21.1-p11.2". European Journal of Human Genetics. 6 (5): 459–66. doi: 10.1038/sj.ejhg.5200207 . PMID   9801870.
• Stoddart KL, Jermak C, Nagaraja R, Schlessinger D, Bech-Hansen NT (February 1999). "Physical map covering a 2 Mb region in human xp11.3 distal to DX6849". Gene. 227 (1): 111–6. doi:10.1016/S0378-1119(98)00564-2. PMID   9931462.
• Thiselton DL, McDowall J, Brandau O, Ramser J, d'Esposito F, Bhattacharya SS, Ross MT, Hardcastle AJ, Meindl A (April 2002). "An integrated, functionally annotated gene map of the DXS8026-ELK1 interval on human Xp11.3-Xp11.23: potential hotspot for neurogenetic disorders". Genomics. 79 (4): 560–72. doi:10.1006/geno.2002.6733. PMID   11944989.
• Ideguchi H, Ueda A, Tanaka M, Yang J, Tsuji T, Ohno S, Hagiwara E, Aoki A, Ishigatsubo Y (October 2002). "Structural and functional characterization of the USP11 deubiquitinating enzyme, which interacts with the RanGTP-associated protein RanBPM". The Biochemical Journal. 367 (Pt 1): 87–95. doi:10.1042/BJ20011851. PMC   1222860 . PMID   12084015.
• Angelats C, Wang XW, Jermiin LS, Copeland NG, Jenkins NA, Baker RT (January 2003). "Isolation and characterization of the mouse ubiquitin-specific protease Usp15". Mammalian Genome. 14 (1): 31–46. doi:10.1007/s00335-002-3035-0. PMID   12532266. S2CID   23215004.
• Bouwmeester T, Bauch A, Ruffner H, Angrand PO, Bergamini G, Croughton K, Cruciat C, Eberhard D, Gagneur J, Ghidelli S, Hopf C, Huhse B, Mangano R, Michon AM, Schirle M, Schlegl J, Schwab M, Stein MA, Bauer A, Casari G, Drewes G, Gavin AC, Jackson DB, Joberty G, Neubauer G, Rick J, Kuster B, Superti-Furga G (February 2004). "A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway". Nature Cell Biology. 6 (2): 97–105. doi:10.1038/ncb1086. PMID   14743216. S2CID   11683986.
• Schoenfeld AR, Apgar S, Dolios G, Wang R, Aaronson SA (September 2004). "BRCA2 is ubiquitinated in vivo and interacts with USP11, a deubiquitinating enzyme that exhibits prosurvival function in the cellular response to DNA damage". Molecular and Cellular Biology. 24 (17): 7444–55. doi:10.1128/MCB.24.17.7444-7455.2004. PMC   506974 . PMID   15314155.
• Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. doi:10.1038/msb4100134. PMC   1847948 . PMID   17353931.