Vitellin

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Vitellin is a protein found in the egg yolk. It is a phosphoprotein. [1] Vitellin is a generic name for major of many yolk proteins. [2]

Contents

Vitellin has been known since the 1900s. [3] The periodic acid-Schiff method and Sudan black B dye was used to help determine that Vitellin is a glycolipoprotein because it stained positive when tested. This protein was found to weigh ~540 kDa, given the weight of each of its 4 major subunits. [4]

Vitellin is essential in the fertilization process, and embryonic development in egg-laying organisms.

This phosphoprotein acts as a membrane, 1-3.5μm, that encloses the egg that comprises at least five glycoproteins that resemble the zona pellucida evident in mammalian organisms. When the egg is fertilized, it buds off from the gamete surface, which results in the fertilization of the membrane in most invertebrates, amphibians, birds, and fishes. During fertilization, the acrosome of the sperm interacts with the vitelline envelope that has species-specific recognition and binding for the sperm. The vitelline membrane consists of two major layers found below the ovary and the outer layer found in the oviduct. This membrane supports the yolk and separates from the albumen, or egg white. The proteins that primarily compose the vitelline membrane are the lysozyme and ovomucin foundational for membrane growth during embryonic development. Aside from structural functions, it is also a barrier that permits the diffusion of water and nutrients, and in chickens especially, it is a barrier against microbial infection. Vitellin comprises a vast fraction of the proteins found in eggs, and due to this, they are easily characterized with biochemical methods in order to elucidate molecular, developments, and physiological regulation studies.

Vitellin was studied in honey bees and was found that it contributed to the health of the embryo in the form of immunity in the embryonic stages. This immunity came from two of the four domains of this protein structure. The honey bees, the domain of unknown function 1943 (DUF1943), and the von Willebrand factor (vWF) type D domain were linked to pathogen recognition and increased immunity in the embryonic honey bees. [5]

Allergen

Vitellin is an umbrella term for many vitellin sub proteins and some of these sub proteins are linked with egg allergies. [6]

See also

Related Research Articles

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Vitellogenin is a precursor of egg yolk that transports protein and some lipid from the liver through the blood to the growing oocytes where it becomes part of the yolk. Normally, it is only found in the blood or hemolymph of females, and can therefore be used as a biomarker in vertebrates of exposure to environmental estrogens which stimulate elevated levels in males as well as females. "Vitellogenin" is a synonymous term for the gene and the expressed protein. The protein product is classified as a glycolipoprotein, having properties of a sugar, fat and protein. It belongs to a family of several lipid transport proteins.

The vitelline membrane or vitelline envelope is a structure surrounding the outer surface of the plasma membrane of an ovum or, in some animals, the extracellular yolk and the oolemma. It is composed mostly of protein fibers, with protein receptors needed for sperm binding which, in turn, are bound to sperm plasma membrane receptors. The species-specificity between these receptors contributes to prevention of breeding between different species. It is called zona pellucida in mammals. Between the vitelline membrane and zona pellucida is a fluid-filled perivitelline space.

Vitelline may refer to:

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Zona pellucida sperm-binding protein 2 is a protein that in humans is encoded by the ZP2 gene.

Oocyteactivation is a series of processes that occur in the oocyte during fertilization.

Egg lysin is a protein that creates a hole in the envelope of the egg thereby allowing the sperm to pass through the envelope and fuse with the egg.

References

  1. Vitellins at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
  2. KUNKEL, JOSEPH G.; JOHN H. NORDIN (1985). "Yolk Proteins. 2.2 Vitellin, the major yolk protein" (PDF). Retrieved 5 November 2016.
  3. CALVERY, HERBERT O.; ABRAHAM WHITE (31 October 1931). "Vitellin of Hen's Egg" (PDF). Journal of Biological Chemistry. 94 (3): 635. doi: 10.1016/S0021-9258(18)76387-4 . Retrieved 5 November 2016.
  4. QUINITIO, EMILIA T.; HARA, AKIHIKO; YAMAUCHI, KOHEI; FUJI, AKIRA (1 June 1990). "Isolation and characterization of vitellin from the ovary of Penaeus monodon". Invertebrate Reproduction & Development. 17 (3): 221–227. Bibcode:1990InvRD..17..221Q. doi:10.1080/07924259.1990.9672114. ISSN   0792-4259.
  5. Leipart, Vilde; Montserrat-Canals, Mateu; Cunha, Eva S.; Luecke, Hartmut; Herrero-Galán, Elías; Halskau, Øyvind; Amdam, Gro V. (January 2022). "Structure prediction of honey bee vitellogenin: a multi-domain protein important for insect immunity". FEBS Open Bio. 12 (1): 51–70. doi:10.1002/2211-5463.13316. ISSN   2211-5463. PMC   8727950 . PMID   34665931.
  6. Puglisi, Gregory; Frieri, Marianne (1 November 2007). "Update on hidden food allergens and food labeling" . Allergy and Asthma Proceedings. 28 (6): 634–639. doi:10.2500/aap.2007.6.3066. ISSN   1088-5412. PMID   18201426.

Hagedorn, H. H., and J. G. Kunkel. "Vitellogenin and vitellin in insects." Annual review of entomology 24.1 (1979): 475-505. https://doi.org/10.1146/annurev.en.24.010179.002355

Zhu, Jiang, Leslie S. Indrasith, and Okitsugu Yamashita. "Characterization of vitellin, egg-specific protein and 30 kDa protein from Bombyx eggs, and their fates during oogenesis and embryogenesis." Biochimica et Biophysica Acta (BBA) - General Subjects 882.3 (1986): 427-436. https://doi.org/10.1016/0304-4165(86)90267-9