YARS

This article is about the enzyme. For the U.S. military installation, see Youngstown Air Reserve Station. For the Russian intercontinental ballistic missile, see RS-24 Yars.

YARS1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1N3L, 1NTG, 1Q11, 4Q93, 4QBT
Identifiers
Aliases	YARS1 , CMTDIC, TYRRS, YRS, YTS, tyrosyl-tRNA synthetase, tyrosyl-tRNA synthetase 1, YARS, IMNEPD2
External IDs	OMIM: 603623 MGI: 2147627 HomoloGene: 2730 GeneCards: YARS1
Gene location (Human) Chr. Chromosome 1 (human) [1] Band 1p35.1 Start 32,775,237 bp [1] End 32,818,031 bp [1]
Gene location (Mouse) Chr. Chromosome 4 (mouse) [2] Band 4|4 D2.2 Start 129,083,553 bp [2] End 129,113,400 bp [2]
RNA expression pattern Bgee Top expressed in Right adrenal gland islet of Langerhans Left adrenal gland prefrontal cortex endothelial cell Stromal cell of endometrium caudate nucleus putamen upper lobe of left lung Brodmann area 9 More reference expression data BioGPS More reference expression data
Gene ontology Molecular function aminoacyl-tRNA ligase activity nucleotide binding signal transducer activity ligase activity GO:0001948 protein binding interleukin-8 receptor binding ATP binding tyrosine-tRNA ligase activity tRNA binding RNA binding Cellular component extracellular space cytoplasm nuclear body cytosol aminoacyl-tRNA synthetase multienzyme complex methionyl glutamyl tRNA synthetase complex Biological process tyrosyl-tRNA aminoacylation protein biosynthesis apoptotic process signal transduction tRNA aminoacylation for protein translation Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	8565	107271
Ensembl	ENSG00000134684	ENSMUSG00000028811
UniProt	P54577	Q91WQ3
RefSeq (mRNA)	NM_003680	NM_134151
RefSeq (protein)	NP_003671	NP_598912
Location (UCSC)	Chr 1: 32.78 – 32.82 Mb	Chr 4: 129.08 – 129.11 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Tyrosyl-tRNA synthetase, cytoplasmic, also known as Tyrosine-tRNA ligase , is an enzyme that in humans is encoded by the YARS gene. ^{[5] [6] [7]}

Living cells translate DNA sequences into RNA sequences and then into protein sequences. Proteins are chains of amino acids, such as tyrosine. As the protein grows, each amino acid is added to the end by an enzyme called transfer RNA (tRNA). Each amino acid has its own tRNA, and tyrosyl-tRNA synthetase is the tRNA that adds tyrosine to the end of a growing protein.

Aminoacyl-tRNA synthetases catalyze the aminoacylation of transfer RNA (tRNA) by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine. ^[7]

Recently, tyrosyl-tRNA synthetase has been demonstrated as the biologically and functionally significant target for resveratrol. ^[8]

For a comparison of cytoplasmic human tyrosyl-tRNA synthetase with its mitochondrial counterpart and with tyrosyl-tRNA synthetases of other biological kingdoms and organisms, see the Wikipedia page on Tyrosine-tRNA ligase and a general review on their structures and functions. ^[9]

References

1. GRCh38: Ensembl release 89: ENSG00000134684 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000028811 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Ribas de Pouplana L, Frugier M, Quinn CL, Schimmel P (Feb 1996). "Evidence that two present-day components needed for the genetic code appeared after nucleated cells separated from eubacteria". Proc Natl Acad Sci U S A. 93 (1): 166–70. Bibcode:1996PNAS...93..166R. doi: 10.1073/pnas.93.1.166 . PMC   40199 . PMID   8552597.
6. Kleeman TA, Wei D, Simpson KL, First EA (Jun 1997). "Human tyrosyl-tRNA synthetase shares amino acid sequence homology with a putative cytokine". J Biol Chem. 272 (22): 14420–5. doi: 10.1074/jbc.272.22.14420 . PMID   9162081.
7. "Entrez Gene: YARS tyrosyl-tRNA synthetase".
8. Sajish, Mathew; Schimmel, Paul (2015). "A human tRNA synthetase is a potent PARP1-activating effector target for resveratrol". Nature. 519 (7543): 370–373. Bibcode:2015Natur.519..370S. doi:10.1038/nature14028. PMC   4368482 . PMID   25533949.
9. Bedouelle, Hugues (2013). Tyrosyl-tRNA Synthetases. In: Madame Curie Bioscience Database [NCBI NBK6553]. Austin (TX): Landes Bioscience.

Further reading

• Ewalt KL, Schimmel P (2002). "Activation of angiogenic signaling pathways by two human tRNA synthetases". Biochemistry. 41 (45): 13344–9. doi:10.1021/bi020537k. PMID   12416978.
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID   8125298.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID   9373149.
• Wakasugi K, Quinn CL, Tao N, Schimmel P (1998). "Genetic code in evolution: switching species-specific aminoacylation with a peptide transplant". EMBO J. 17 (1): 297–305. doi:10.1093/emboj/17.1.297. PMC   1170380 . PMID   9427763.
• Wakasugi K, Schimmel P (1999). "Two distinct cytokines released from a human aminoacyl-tRNA synthetase". Science. 284 (5411): 147–51. Bibcode:1999Sci...284..147W. doi:10.1126/science.284.5411.147. PMID   10102815.
• Wakasugi K, Schimmel P (1999). "Highly differentiated motifs responsible for two cytokine activities of a split human tRNA synthetase". J. Biol. Chem. 274 (33): 23155–9. doi: 10.1074/jbc.274.33.23155 . PMID   10438485.
• Austin J, First EA (2002). "Catalysis of tyrosyl-adenylate formation by the human tyrosyl-tRNA synthetase". J. Biol. Chem. 277 (17): 14812–20. doi: 10.1074/jbc.M103396200 . PMID   11856731.
• Austin J, First EA (2002). "Potassium functionally replaces the second lysine of the KMSKS signature sequence in human tyrosyl-tRNA synthetase". J. Biol. Chem. 277 (23): 20243–8. doi: 10.1074/jbc.M201923200 . PMID   11927599.
• Wakasugi K, Slike BM, Hood J, et al. (2002). "Induction of angiogenesis by a fragment of human tyrosyl-tRNA synthetase". J. Biol. Chem. 277 (23): 20124–6. doi: 10.1074/jbc.C200126200 . PMID   11956181.
• Austin J, First EA (2002). "Comparison of the catalytic roles played by the KMSKS motif in the human and Bacillus stearothermophilus trosyl-tRNA synthetases". J. Biol. Chem. 277 (32): 28394–9. doi: 10.1074/jbc.M204404200 . PMID   12016229.
• Yang XL, Skene RJ, McRee DE, Schimmel P (2003). "Crystal structure of a human aminoacyl-tRNA synthetase cytokine". Proc. Natl. Acad. Sci. U.S.A. 99 (24): 15369–74. doi: 10.1073/pnas.242611799 . PMC   137723 . PMID   12427973.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Jia J, Li B, Jin Y, Wang D (2003). "Expression, purification, and characterization of human tyrosyl-tRNA synthetase". Protein Expr. Purif. 27 (1): 104–8. doi:10.1016/S1046-5928(02)00576-4. PMID   12509991.
• Gevaert K, Goethals M, Martens L, et al. (2004). "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides". Nat. Biotechnol. 21 (5): 566–9. doi:10.1038/nbt810. PMID   12665801. S2CID   23783563.
• Jordanova A, Thomas FP, Guergueltcheva V, et al. (2004). "Dominant intermediate Charcot-Marie-Tooth type C maps to chromosome 1p34-p35". Am. J. Hum. Genet. 73 (6): 1423–30. doi:10.1086/379792. PMC   1180404 . PMID   14606043.
• Yang XL, Otero FJ, Skene RJ, et al. (2004). "Crystal structures that suggest late development of genetic code components for differentiating aromatic side chains". Proc. Natl. Acad. Sci. U.S.A. 100 (26): 15376–80. doi: 10.1073/pnas.2136794100 . PMC   307575 . PMID   14671330.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi: 10.1038/ng1285 . PMID   14702039.

External links

• Overview of all the structural information available in the PDB for UniProt : P54577 (Tyrosine--tRNA ligase, cytoplasmic) at the PDBe-KB .