TUT7 | |||||||||||||||||||||||||||||||||||||||||||||||||||
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Aliases | TUT7 , PAPD6, zinc finger CCHC-type containing 6, TENT3B, ZCCHC6, terminal uridylyl transferase 7 | ||||||||||||||||||||||||||||||||||||||||||||||||||
External IDs | OMIM: 613467 MGI: 2387179 HomoloGene: 51941 GeneCards: TUT7 | ||||||||||||||||||||||||||||||||||||||||||||||||||
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Wikidata | |||||||||||||||||||||||||||||||||||||||||||||||||||
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Terminal uridylyltransferase 7 (TUT7), also known as "zinc finger, CCHC domain containing 6", is an enzyme that in humans is encoded by the ZCCHC6 gene located on chromosome 9. [5] [6] The ZCCHC6 protein mediates the terminal uridylation of RNA transcripts with short poly-A tails and is involved in mRNA and microRNA degradation
The ZCCHC6 gene contains 33 exons with at least six known isoforms due to alternative splicing. The ZCCHC6 gene encodes for a protein that is 171 kDa in molecular weight and is localized to the cytoplasm.
It catalyzes the following reaction, requiring Mg2+ and Mn2+ as co-factors.
UTP + RNA(n) = diphosphate + RNA(n+1) [7]
Uridylation catalyzed by ZCCHC6 takes place readily on deadenylated mRNAs inside the cells. [8] Purified ZZHC6 selectively recognizes and uridylates RNA molecules possessing short poly(A) tails (less than 25 nucleotides) in vitro. In cells depleted of ZCCHC6, the majority of mRNAs lose the signature oligo(U) tails that are characteristic of ZCCHC6 reactivity, and the half-life of mRNA molecules are accordingly prolonged. [8]
In addition to mRNA degradation, uridylation is also thought to function in pre-microRNA maturation, with some group II pre-microRNA requiring 3' mono-uridylation for Dicer processing. [9] ZCCHC6 is thought to work in redundancy with ZCCHC11 to mediate the biogenesis of the let-7 microRNA through uridylation. [10]
Genetic Inactivation of ZCCHC6 Suppresses Interleukin-6 Expression and Reduces the Severity of Experimental Osteoarthritis in Mice. [11]
Cleavage and polyadenylation specificity factor subunit 5 (CPSF5) is an enzyme that in humans is encoded by the NUDT21 gene. It belongs to the Nudix family of hydrolases.
Spermatid perinuclear RNA-binding protein is a protein that in humans is encoded by the STRBP gene.
Deubiquitinating protein VCIP135 is a protein that in humans is encoded by the VCPIP1 gene.
Polyadenylate-binding protein-interacting protein 2 is a protein that in humans is encoded by the PAIP2 gene.
Myotubularin-related protein 6 is a protein that in humans is encoded by the MTMR6 gene.
Zinc finger CCHC domain-containing protein 2 is a protein that in humans is encoded by the ZCCHC2 gene.
Poly [ADP-ribose] polymerase 8 is an enzyme that in humans is encoded by the PARP8 gene.
LisH domain-containing protein ARMC9 is a protein that in humans is encoded by the ARMC9 gene.
Transmembrane protein 47 is a protein that in humans is encoded by the TMEM47 gene.
TCDD-inducible poly [ADP-ribose] polymerase is an enzyme that in humans is encoded by the TIPARP gene.
N-acetyltransferase 9 is an enzyme that in humans is encoded by the NAT9 gene.
Synaptotagmin-13 is a protein that in humans is encoded by the SYT13 gene.
RNA-binding protein MEX3B is a protein that in humans is encoded by the MEX3B gene.
Zinc finger protein 346 is a protein that in humans is encoded by the ZNF346 gene.
Mitochondrial pyruvate carrier 2 (MPC2) also known as brain protein 44 (BRP44) is a protein that in humans is encoded by the MPC2 gene. It is part of the Mitochondrial Pyruvate Carrier (MPC) protein family. This protein is involved in transport of pyruvate across the inner membrane of mitochondria in preparation for the pyruvate dehydrogenase reaction.
Zinc finger protein 471 is a protein that in humans is encoded by the ZNF471 gene.
DEP domain-containing mTOR-interacting protein (DEPTOR) also known as DEP domain-containing protein 6 (DEPDC6) is a protein that in humans is encoded by the DEPTOR gene.
Zinc finger protein 34 is a protein that in humans is encoded by the ZNF34 gene.
Integrator complex subunit 7 is a protein that in humans is encoded by the INTS7 gene.
Sodium/bile acid cotransporter 7 is a protein which in humans is encoded by the SLC10A7 gene.