Serena Williams was the defending champion, but withdrew due to injury.
Players and pairs who neither have high enough rankings nor receive wild cards may participate in a qualifying tournament held one week before the annual Wimbledon Tennis Championships.
Alona Bondarenko was the defending champion, but chose not to participate this year due to a wrist injury.
6th seed Jarmila Groth won in the final 6–4, 6–3, against Bethanie Mattek-Sands.
Jelena Janković was the defending champion, but lost to compatriot Ana Ivanovic in the fourth round.
Li Na was the defending champion, but lost the final against Victoria Azarenka, 6–2, 1–6, 6–3.
Caroline Wozniacki was the defending champion, but she chose not to participate.
Agnieszka Radwańska won the title, defeating Simona Halep 7–5, 6–0 in the final.
Petra Kvitová was the defending champion, but could not participate as Victoria Azarenka participated.
Serena Williams successfully defended her title by defeating Maria Sharapova in the final, 6–1, 6–4.
Petra Kvitová was the defending champion, but she lost to Sorana Cîrstea in the quarterfinals.
Agnieszka Radwańska was the defending champion, but she lost in the second round to Bethanie Mattek-Sands.
Petra Kvitová was the defending champion, but lost in the second round to Carla Suárez Navarro.
Victoria Azarenka was the defending champion, but withdrew because of a right knee injury.
Simona Halep was the defending champion, but lost in the second round to Magdaléna Rybáriková.
Serena Williams was the defending champion, but withdrew before her quarterfinal match with a knee injury.
Petra Kvitová was the defending champion, but lost in the third round to Daria Gavrilova.
Belinda Bencic was the defending champion, but withdrew with a left wrist injury before the tournament began.
Venus Williams was the defending champion, but lost in the third round to Svetlana Kuznetsova.
Elina Svitolina was the defending champion and successfully defended her title, defeating Simona Halep in a rematch of the previous year's final, 6–0, 6–4.
Gabriela Dabrowski and Xu Yifan were the defending champions, but lost in the first round to Simona Halep and Raluca Olaru.
Garbiñe Muguruza defeated Barbora Krejčíková in the final, 7–6(8–6), 6–3 to win the women's singles tennis event at the 2021 Dubai Tennis Championships. Muguruza dropped just one set the entire tournament, to Aryna Sabalenka in the quarterfinals.
Renata Voráčová (second round)
Anna Lapushchenkova (first round)
Edina Gallovits (second round)
Arantxa Rus (second round)
Zuzana Kučová (qualifying competition)
Bojana Jovanovski (second round)
Michelle Larcher de Brito (second round)
Kaia Kanepi (qualified)
Valérie Tétreault (first round)
Maša Zec Peškirič (first round)
Bethanie Mattek-Sands (qualifying competition, lucky loser)
Patricia Mayr (first round)
Zhang Shuai (qualified)
Chanelle Scheepers (qualified)
Kathrin Wörle (qualifying competition)
Sesil Karatantcheva (first round)
