| 2023 Challenger Ciudad de Guayaquil | |
|---|---|
| Date | 30 October – 5 November |
| Edition | 19th |
| Surface | Clay |
| Location | Guayaquil, Ecuador |
| Champions | |
| Singles | |
 Alejandro Tabilo | |
| Doubles | |
 Arklon Huertas del Pino / Conner Huertas del Pino | |
The 2023 Challenger Ciudad de Guayaquil was a professional tennis tournament played on clay courts. It was the 19th edition of the tournament which was part of the 2023 ATP Challenger Tour. It took place in Guayaquil, Ecuador between 30 October and 5 November 2023.
| Country | Player | Rank1 | Seed |
|---|---|---|---|
 ARG | Federico Coria | 76 | 1 |
 COL | Daniel Elahi Galán | 98 | 2 |
| ARG | Facundo Díaz Acosta | 109 | 3 |
| ARG | Juan Manuel Cerúndolo | 114 | 4 |
| CHI | Alejandro Tabilo | 122 | 5 |
 BOL | Hugo Dellien | 129 | 6 |
 BRA | Felipe Meligeni Alves | 144 | 7 |
| ARG | Camilo Ugo Carabelli | 153 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
Gonzalo Bueno 
Gerard Campana Lee
Max Houkes Nicolás Mejía 
Carlos Taberner Juan Bautista Torres The following player received entry as a lucky loser:
Alejandro Tabilo def. Daniel Elahi Galán 6–2, 6–2. Arklon Huertas del Pino / Conner Huertas del Pino def. 
Luca Margaroli / Santiago Rodríguez Taverna 6–3, 6–1.