| 2023 Cali Open | |
|---|---|
| Date | 19 – 24 June |
| Edition | 2nd |
| Surface | Clay |
| Location | Cali, Colombia |
| Champions | |
| Singles | |
 Federico Delbonis | |
| Doubles | |
Guido Andreozzi /  Cristian Rodríguez | |
The 2023 Cali Open was a professional tennis tournament played on clay courts. It was the second edition of the tournament which was part of the 2023 ATP Challenger Tour. It took place in Cali, Colombia between 19 and 24 June 2023.
| Country | Player | Rank1 | Seed |
|---|---|---|---|
 CHI | Alejandro Tabilo | 148 | 1 |
| ARG | Román Andrés Burruchaga | 222 | 2 |
 CAN | Alexis Galarneau | 224 | 3 |
| COL | Nicolás Mejía | 247 | 4 |
| ARG | Santiago Rodríguez Taverna | 249 | 5 |
| ARG | Federico Delbonis | 264 | 6 |
 BRA | João Lucas Reis da Silva | 265 | 7 |
| ARG | Guido Andreozzi | 279 | 8 |
The following players received wildcards into the singles main draw:
Nicolás Mejía Johan Alexander Rodríguez Adrià Soriano BarreraThe following players received entry into the singles main draw using protected rankings:
The following players received entry into the singles main draw as alternates:
The following players received entry from the qualifying draw:
Pedro Boscardin Dias Alejandro Hoyos Gonzalo Lama Juan Ignacio Londero Ignacio Monzón Matías Soto Federico Delbonis def. Guido Andreozzi 6–4, 6–7(6–8), 6–3. Guido Andreozzi / Cristian Rodríguez def. Orlando Luz / 
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