The 40th General Assembly of Prince Edward Island was in session from March 12, 1924, to June 2, 1927. The Conservative Party led by James David Stewart formed the government.
Louis L. Jenkins was elected speaker.
There were four sessions of the 40th General Assembly:
| Session | Start | End |
|---|---|---|
| 1st | March 12, 1924 | April 11, 1924 |
| 2nd | March 17, 1925 | April 9, 1925 |
| 3rd | March 9, 1926 | March 31, 1926 |
| 4th | March 15, 1927 | April 12, 1927 |
| District | Assemblyman | Party | First elected / previously elected | |
|---|---|---|---|---|
| 1st Kings | Augustine A. MacDonald | Conservative | 1915, 1923 | |
| 2nd Kings | Harvey D. McEwen | Conservative | 1915, 1923 | |
| 3rd Kings | Leslie Hunter | Conservative | 1923 | |
| 4th Kings | Maynard F. McDonald | Conservative | 1923 | |
| 5th Kings | J. Howard MacDonald | Conservative | 1923 | |
| District | Councillor | Party | First elected / previously elected | |
| 1st Kings | Harry D. McLean | Conservative | 1916 | |
| 2nd Kings | James B. McDonald | Conservative | 1923 | |
| 3rd Kings | John Alexander Macdonald [1] | Conservative | 1908, 1923 | |
| H. Frank McPhee (1926) | Independent | 1926 | ||
| 4th Kings | Albert P. Prowse [2] | Conservative | 1899, 1904, 1923 | |
| Norman MacLeod (1926) | Conservative | 1926 | ||
| 5th Kings | James David Stewart | Conservative | 1917 |
| District | Assemblyman | Party | First elected / previously elected | |
|---|---|---|---|---|
| 1st Prince | Jeremiah Blanchard | Liberal | 1922 | |
| 2nd Prince | Albert Charles Saunders | Liberal | 1915 | |
| 3rd Prince | Adrien Arsenault | Conservative | 1922 | |
| 4th Prince | Whitefield Bentley | Conservative | 1923 | |
| 5th Prince | James A. MacNeill | Conservative | 1908, 1923 | |
| District | Councillor | Party | First elected / previously elected | |
| 1st Prince | Wilfred Tanton | Conservative | 1923 | |
| 2nd Prince | William H. Dennis | Liberal | 1915 | |
| 3rd Prince | Thomas MacNutt | Conservative | 1922 | |
| 4th Prince | John H. Myers | Conservative | 1912, 1923 | |
| 5th Prince | Creelman McArthur [3] | Liberal | 1919 | |
| George D. Pope(1926) | Conservative | 1926 |
| District | Assemblyman | Party | First elected / previously elected | |
|---|---|---|---|---|
| 1st Queens | Murdock Kennedy | Conservative | 1906 | |
| 2nd Queens | John Buntain | Conservative | 1912, 1923 | |
| 3rd Queens | Leonard J. Wood | Conservative | 1904, 1915, 1923 | |
| 4th Queens | James C. Irving | Liberal | 1919 | |
| 5th Queens | W. Chester S. McLure | Conservative | 1923 | |
| District | Councillor | Party | First elected / previously elected | |
| 1st Queens | Alexander McNevin | Conservative | 1915, 1923 | |
| 2nd Queens | Louis Jenkins | Conservative | 1912, 1923 | |
| 3rd Queens | J. Augustine MacDonald | Conservative | 1923 | |
| 4th Queens | Shaw McMillan | Conservative | 1923 | |
| 5th Queens | William Joseph Parnell MacMillan | Conservative | 1923 |
Notes: