Bacterial adhesin

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Bacterial adhesins are cell-surface components or appendages of bacteria that facilitate adhesion or adherence to other cells or to surfaces, usually in the host they are infecting or living in. Adhesins are a type of virulence factor.

Contents

Adherence is an essential step in bacterial pathogenesis or infection, required for colonizing a new host. [1] Adhesion and bacterial adhesins are also a potential target either for prophylaxis or for the treatment of bacterial infections. [2]

Background

Bacteria are typically found attached to and living in close association with surfaces. During the bacterial lifespan, a bacterium is subjected to frequent shear-forces. In the crudest sense, bacterial adhesins serve as anchors allowing bacteria to overcome these environmental shear forces, thus remaining in their desired environment. However, bacterial adhesins do not serve as a sort of universal bacterial Velcro. Rather, they act as specific surface recognition molecules, allowing the targeting of a particular bacterium to a particular surface such as root tissue in plants, lacrimal duct tissues in mammals, or even tooth enamel. [3]

FimH is a bacterial adhesin that helps bacteria such as Escherichia coli to bind to host cells and their receptors (here: the human proteins CD48 and TLR4, or mannose residues). Bacterial Adhesin FimH - host interaction.png
FimH is a bacterial adhesin that helps bacteria such as Escherichia coli to bind to host cells and their receptors (here: the human proteins CD48 and TLR4, or mannose residues).

Most fimbria of gram-negative bacteria function as adhesins, but in many cases it is a minor subunit protein at the tip of the fimbriae that is the actual adhesin. In gram-positive bacteria, a protein or polysaccharide surface layer serves as the specific adhesin. To effectively achieve adherence to host surfaces, many bacteria produce multiple adherence factors called adhesins.

Bacterial adhesins provide species and tissue tropism. Adhesins are expressed by both pathogenic bacteria and saprophytic bacteria. This prevalence marks them as key microbial virulence factors in addition to a bacterium's ability to produce toxins and resist the immune defenses of the host.

Structures

Through the mechanisms of evolution, different species of bacteria have developed different solutions to the problem of attaching receptor specific proteins to the bacteria surface. Today many different types and subclasses of bacterial adhesins may be observed in the literature.

The typical structure of a bacterial adhesin is that of a fimbria or pilus. [3] The bacterial adhesin consists primarily of an intramembranous structural protein which provides a scaffold upon which several extracellular adhesins may be attached. [3] However, as in the case of the CFA1 fimbriae, the structural protein itself can sometimes act as an adhesin if a portion of the protein extends into the ECM.

FimH adhesin—structure

The best characterized bacterial adhesin is the type 1 fimbrial FimH adhesin. This adhesin is responsible for D-mannose sensitive adhesion. [3] The bacterium synthesizes a precursor protein consisting of 300 amino acids then processes the protein by removing several signal peptides ultimately leaving a 279 amino acid protein. [3] Mature FimH is displayed on the bacterial surface as a component of the type 1 fimbrial organelle. [3]

In 1999, the structure of FimH was resolved via x-ray crystallography. FimH is folded into two domains. The N terminal adhesive domain plays the main role in surface recognition while the C-terminal domain is responsible for organelle integration. [5] A tetra-peptide loop links the two domains. Additionally, a carbohydrate-binding pocket has been identified at the tip of the N-terminal adhesive domain. [5] This basic structure is conserved across type 1 fimbrial adhesins though recent studies have shown that in vitro induced mutations can lead to the addition of C-terminal domain specificity resulting in a bacterial adhesion with dual bending sites and related binding phenotypes. [6]

As virulence factors

The majority of bacterial pathogens exploit specific adhesion to host cells as their main virulence factor. "A large number of bacterial adhesins with individual receptor specificities have been identified." [3] Many bacterial pathogens are able to express an array of different adhesins. Expression of these adhesins at different phases during infection play the most important role in adhesion based virulence. [3] Numerous studies have shown that inhibiting a single adhesin in this coordinated effort can often be enough to make a pathogenic bacterium non-virulent. This has led to the exploration of adhesin activity interruption as a method of bacterial infection treatment.

Vaccines based on adhesins

The study of adhesins as a point of exploitation for vaccines comes from early studies which indicated that an important component of protective immunity against certain bacteria came from an ability to prevent adhesin binding. [7] Additionally, adhesins are attractive vaccine candidates because they are often essential to infection and are surface-located, making them readily accessible to antibodies.

The effectiveness of anti-adhesin antibodies is illustrated by studies with FimH, the adhesin of uropathogenic Escherichia coli (UPEC). Work with E. coli stems from observations of human acquired immunity. Children in third world countries may suffer from several episodes of E. coli associated diarrhea during the first three years of life. If the child survives this initial period of susceptibility, infection rates typically drop substantially. Field studies show that this acquired immunity is directed primarily against bacterial adhesins. [3]

Recent studies from Worcester Polytechnic Institute show that the consumption of cranberry juice may inhibit the action of UPEC adhesins. Using atomic force microscopy researchers have shown that adhesion forces decrease with time following cranberry juice consumption. [8] This research has opened the door to further exploration of orally administered vaccines which exploit bacterial adhesins.

A number of problems create challenges for the researcher exploring the anti-adhesin immunity concept. First, a large number of different bacterial adhesins target the same human tissues. Further, an individual bacterium can produce multiple different types of adhesin, at different times, in different places, and in response to different environmental triggers. [3] Finally, many adhesins present as different immunologically distinct antigenic varieties, even within the same clone (as is the case in Neisseria gonorrhoeae ). [9]

Despite these challenges, progress is being made in the creation of anti-adhesion vaccines. In animal models, passive immunization with anti FimH-antibodies and vaccination with the protein significantly reduced colonization by UPEC. [10] Moreover, the Bordetella pertussis adhesins FHA and pertactin are components of three of the four acellular pertussis vaccines currently licensed for use in the U.S. Additionally, anti-adhesion vaccines are being explored as a solution to urinary tract infection (UTIs). The use of synthetic FimH adhesion peptides was shown to prevent urogenital mucosal infection by E. coli in mice. [11]

Specific examples

Dr family

Adhesin_Dr
PDB 1ut1 EBI.jpg
drae adhesin from escherichia coli
Identifiers
SymbolAdhesin_Dr
Pfam PF04619
Pfam clan CL0204
InterPro IPR006713
Available protein structures:
Pfam   structures / ECOD  
PDB RCSB PDB; PDBe; PDBj
PDBsum structure summary

The Dr family of adhesins bind to the Dr blood group antigen component of decay-accelerating factor (DAF). [12] These proteins contain both fimbriated and afimbriated adherence structures and mediate adherence of uropathogenic Escherichia coli to the urinary tract. [13] They do so by inducing the development of long cellular extensions that wrap around the bacteria. [12] They also confer the mannose-resistant hemaglutination phenotype, which can be inhibited by chloramphenicol. The N-terminal portion of the mature protein is thought to be responsible for chloramphenicol sensitivity. [14] Also, they induce activation of several signal transduction cascades, including activation of PI-3 kinase. [12]

The Dr family of adhesins are particularly associated with cystitis and pregnancy-associated pyelonephritis. [12]

Multivalent adhesion molecules

Multivalent adhesion molecules (MAMs) are a widespread family of adhesins found in Gram negative bacteria, including E. coli, Vibrio, Yersinia, and Pseudomonas aeruginosa. [15] MAMs contain tandem repeats of mammalian cell entry (MCE) domains which specifically bind to extracellular matrix proteins and anionic lipids on host tissues. Since they are abundant in many pathogens of clinical importance, multivalent adhesion molecules are a potential target for prophylactic or therapeutic anti-infectives. The use of a MAM targeting adhesion inhibitor was shown to significantly decrease the colonization of burn wounds by multidrug resistant Pseudomonas aeruginosa in rats. [16]

N. gonorroheae

N. gonorrhoeae is host restricted almost entirely to humans. [3] "Extensive studies have established type 4 fimbrial adhesins of N. gonorrhoeae virulence factors." [3] These studies have shown that only strains capable of expressing fimbriae are pathogenic. High survival of polymorphonuclear neutrophils (PMNs) characterizes Neisseria gonorrhoeae infections. Additionally, recent studies out of Stockholm have shown that Neisseria can hitchhike on PMNs using their adhesin pili thus hiding them from neutrophil phagocytic activity. This action facilitates the spread of the pathogen throughout the epithelial cell layer. [17]

E. coli

Escherichia coli strains most known for causing diarrhea can be found in the intestinal tissue of pigs and humans where they express the K88 and CFA1. [18] to attach to the intestinal lining. Additionally, UPEC causes about 90% of urinary tract infections. [19] Of those E. coli which cause UTIs, 95% express type 1 fimbriae. FimH in E. coli overcomes the antibody based immune response by natural conversion from the high to the low affinity state. Through this conversion, FimH adhesion may shed the antibodies bound to it. Escherichia coli FimH provides an example of conformation specific immune response which enhances impact on the protein. [19] By studying this particular adhesion, researchers hope to develop adhesion-specific vaccines which may serve as a model for antibody-mediation of pathogen adhesion. [19]

See also

Related Research Articles

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A pilus is a hair-like cell-surface appendage found on many bacteria and archaea. The terms pilus and fimbria can be used interchangeably, although some researchers reserve the term pilus for the appendage required for bacterial conjugation. All conjugative pili are primarily composed of pilin – fibrous proteins, which are oligomeric.

<i>Neisseria gonorrhoeae</i> Species of bacterium

Neisseria gonorrhoeae, also known as gonococcus (singular) or gonococci (plural), is a species of Gram-negative diplococci bacteria first isolated by Albert Neisser in 1879. An obligate human pathogen, it primarily colonizes the mucosal lining of the urogenital tract; however, it is also capable of adhering to the mucosa of the nose, pharynx, rectum, and conjunctiva. It causes the sexually transmitted genitourinary infection gonorrhea as well as other forms of gonococcal disease including disseminated gonococcemia, septic arthritis, and gonococcal ophthalmia neonatorum.

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<span class="mw-page-title-main">Lipopolysaccharide</span> Class of molecules found in the outer membrane of gram-negative bacteria

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<i>Neisseria meningitidis</i> Species of bacterium that can cause meningitis

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Pathogenic <i>Escherichia coli</i> Strains of E. coli that can cause disease

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<span class="mw-page-title-main">Trimeric autotransporter adhesin</span> Proteins found on the outer membrane of Gram-negative bacteria

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Enteroaggregative Escherichia coli are a pathotype of Escherichia coli which cause acute and chronic diarrhea in both the developed and developing world. They may also cause urinary tract infections. EAEC are defined by their "stacked-brick" pattern of adhesion to the human laryngeal epithelial cell line HEp-2. The pathogenesis of EAEC involves the aggregation of and adherence of the bacteria to the intestinal mucosa, where they elaborate enterotoxins and cytotoxins that damage host cells and induce inflammation that results in diarrhea.

Chaperone-usher fimbriae (CU) are linear, unbranching, outer-membrane pili secreted by gram-negative bacteria through the chaperone-usher system rather than through type IV secretion or extracellular nucleation systems. These fimbriae are built up out of modular pilus subunits, which are transported into the periplasm in a Sec dependent manner. Chaperone-usher secreted fimbriae are important pathogenicity factors facilitating host colonisation, localisation and biofilm formation in clinically important species such as uropathogenic Escherichia coli and Pseudomonas aeruginosa.

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Harry Lee Thompson Mobley, Ph.D, is the Frederick G. Novy Distinguished University Professor of Microbiology and Immunology at the University of Michigan Medical School. His research focused on elucidating the mechanisms by which Gram-negative bacilli that include E. coli, Proteus mirabilis, Klebsiella pneumoniae, Citrobacter freundii, Serratia marcescens, Acinetobacter baumannii, and Helicobacter pylori colonize initial sites of infections that include the urinary tract, the lungs, and the gastrointestinal tract, in some cases, disseminating systemically and entering the bloodstream and the blood-filtering organs including the spleen and liver. For decades, the lab studied urinary tract infection including both “uncomplicated” UTI in otherwise healthy women and “complicated” UTI such as catheter-associated UTI. Bacterial infections of the bladder can ascend to the kidneys and enter renal capillaries to gain access to the bloodstream and infect blood-filtering organs. His research focused on the mechanism by which Gram-negative bacilli colonize the human host, elude the innate immune response, and disseminate from primary sites of infection including the urinary tract into the bloodstream.

References

  1. ↑ Coutte L, Alonso S, Reveneau N, Willery E, Quatannens B, Locht C, Jacob-Dubuisson F (2003). "Role of adhesin release for mucosal colonization by a bacterial pathogen". J Exp Med. 197 (6): 735–42. doi:10.1084/jem.20021153. PMC   2193847 . PMID   12629063.
  2. ↑ Krachler, AM; Orth, K (2014). "Targeting the bacteria-host interface: strategies in anti-adhesion therapy". Virulence. 4 (4): 284–94. doi:10.4161/viru.24606. PMC   3710331 . PMID   23799663.
  3. 1 2 3 4 5 6 7 8 9 10 11 12 Klemm P, Schembri MA (March 2000). "Bacterial adhesins: function and structure". Int. J. Med. Microbiol. 290 (1): 27–35. doi:10.1016/S1438-4221(00)80102-2. PMID   11043979.
  4. ↑ Kline, Kimberly A.; Fälker, Stefan; Dahlberg, Sofia; Normark, Staffan; Henriques-Normark, Birgitta (2009). "Bacterial Adhesins in Host-Microbe Interactions". Cell Host & Microbe. 5 (6): 580–592. doi: 10.1016/j.chom.2009.05.011 . PMID   19527885.
  5. 1 2 Choudhury D, Thompson A, Stojanoff V, et al. (August 1999). "X-ray structure of the FimC-FimH chaperone-adhesin complex from uropathogenic Escherichia coli". Science. 285 (5430): 1061–6. doi:10.1126/science.285.5430.1061. PMID   10446051.
  6. ↑ Schembri MA, Klemm P (May 1998). "Heterobinary adhesins based on the Escherichia coli FimH fimbrial protein". Appl. Environ. Microbiol. 64 (5): 1628–33. Bibcode:1998ApEnM..64.1628S. doi:10.1128/AEM.64.5.1628-1633.1998. PMC   106206 . PMID   9572927.
  7. ↑ Levine, M. M.; Giron, J. A.; Noriega, E. R. (1994). "Fimbrial vaccines". In Klemm, Per (ed.). Fimbriae : adhesion, genetics, biogenesis, and vaccines. Boca Raton: CRC Press. pp. 255–270. ISBN   978-0849348945.
  8. ↑ Tao Y, Pinzón-Arango PA, Howell AB, Camesano TA (2011). "Oral consumption of cranberry juice cocktail inhibits molecular-scale adhesion of clinical uropathogenic Escherichia coli". J Med Food. 14 (7–8): 739–45. doi:10.1089/jmf.2010.0154. PMC   3133681 . PMID   21480803.
  9. ↑ Davies, J. K.; Koomey, J. M.; Seifert, H. S. (1994). "Pili (fimbriae) of Neisseria gonorrhoeae". In Klemm, Per (ed.). Fimbriae : adhesion, genetics, biogenesis, and vaccines. Boca Raton: CRC Press. pp. 147–155. ISBN   978-0849348945.
  10. ↑ Langermann S, Möllby R, Burlein J, Palaszynski S, Auguste C, DeFusco A, Strouse R, Schenerman M, Hultgren S, Pinkner J, Winberg J, Guldevall L, Söderhäll M, Ishikawa K, Normark S, Koenig S (2000). "Vaccination with FimH adhesin protects cynomolgus monkeys from colonization and infection by uropathogenic Escherichia coli". J Infect Dis. 181 (2): 774–8. doi:10.1086/315258. PMID   10669375.
  11. ↑ Langermann S, Palaszynski S, Barnhart M, et al. (April 1997). "Prevention of mucosal Escherichia coli infection by FimH-adhesin-based systemic vaccination". Science. 276 (5312): 607–11. doi:10.1126/science.276.5312.607. PMID   9110982.
  12. 1 2 3 4 Identified Virulence Factors of UPEC : Adherence, State Key Laboratory for Moleclular Virology and Genetic Engineering, Beijing. Retrieved July 2011
  13. ↑ Zhang L, Foxman B, Tallman P, Cladera E, Le Bouguenec C, Marrs CF (June 1997). "Distribution of drb genes coding for Dr binding adhesins among uropathogenic and fecal Escherichia coli isolates and identification of new subtypes". Infection and Immunity. 65 (6): 2011–8. doi:10.1128/iai.65.6.2011-2018.1997. PMC   175278 . PMID   9169726.
  14. ↑ Swanson TN, Bilge SS, Nowicki B, Moseley SL (January 1991). "Molecular structure of the Dr adhesin: nucleotide sequence and mapping of receptor-binding domain by use of fusion constructs". Infection and Immunity. 59 (1): 261–8. doi:10.1128/iai.59.1.261-268.1991. PMC   257736 . PMID   1670929.
  15. ↑ Krachler, Anne Marie; Ham, Hyeilin; Orth, Kim (2011-07-12). "Outer membrane adhesion factor multivalent adhesion molecule 7 initiates host cell binding during infection by gram-negative pathogens". Proceedings of the National Academy of Sciences of the United States of America. 108 (28): 11614–11619. Bibcode:2011PNAS..10811614K. doi: 10.1073/pnas.1102360108 . ISSN   1091-6490. PMC   3136308 . PMID   21709226.
  16. ↑ Huebinger, Ryan M.; Stones, Daniel H.; de Souza Santos, Marcela; Carlson, Deborah L.; Song, Juquan; Vaz, Diana Pereira; Keen, Emma; Wolf, Steven E.; Orth, Kim (2016-12-20). "Targeting bacterial adherence inhibits multidrug-resistant Pseudomonas aeruginosa infection following burn injury". Scientific Reports. 6: 39341. Bibcode:2016NatSR...639341H. doi:10.1038/srep39341. ISSN   2045-2322. PMC   5171828 . PMID   27996032.
  17. ↑ Söderholm N, Vielfort K, Hultenby K, Aro H (2011). "Pathogenic Neisseria hitchhike on the uropod of human neutrophils". PLOS ONE. 6 (9): e24353. Bibcode:2011PLoSO...624353S. doi: 10.1371/journal.pone.0024353 . PMC   3174955 . PMID   21949708.
  18. ↑ Gaastra W, de Graaf FK (June 1982). "Host-specific fimbrial adhesins of noninvasive enterotoxigenic Escherichia coli strains". Microbiol. Rev. 46 (2): 129–61. doi:10.1128/mr.46.2.129-161.1982. PMC   281536 . PMID   6126799.
  19. 1 2 3 Tchesnokova V, Aprikian P, Kisiela D, et al. (October 2011). "Type 1 fimbrial adhesin FimH elicits an immune response that enhances cell adhesion of Escherichia coli". Infect. Immun. 79 (10): 3895–904. doi:10.1128/IAI.05169-11. PMC   3187269 . PMID   21768279.

Adhesins are also used in cell communication, and bind to surface communicators. Can also be used to bind to other bacteria.

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