Brettanomyces claussenii

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Brettanomyces claussenii
Scientific classification OOjs UI icon edit-ltr.svg
Domain: Eukaryota
Kingdom: Fungi
Division: Ascomycota
Class: Saccharomycetes
Order: Saccharomycetales
Family: Pichiaceae
Genus: Brettanomyces
Species:
B. claussenii
Binomial name
Brettanomyces claussenii
Custers

Brettanomyces claussenii (anamorph of Dekkera claussenii) is a wild yeast of the genus Brettanomyces which has a negative Pasteur effect. It and Brettanomyces anomalus share identical mtDNA. In the wild, it is found on the skins of fruit. It has been shown to be useful for wine and beer fermentation as well as ethanol production.

Contents

History

In 1889, Seyffert of the Kalinkin Brewery in St.Petersburg was the first to isolate a " Torula " from English beer which produced the typical "English" taste in lager beer, and in 1899 JW Tullo at Guinness described two types of "secondary yeast" in Irish stout. [1] However, N. Hjelte Claussen at the Carlsberg brewery was the first to publish a description in 1904, following a 1903 patent (UK patent GB190328184) that was the first patented microorganism in history. [2] Claussen named the genus Brettanomyces, which is Greek for British Fungus. For the most part Brettanomyces is viewed as a contaminant, as it forms compounds that lead to 'off-flavors in both wine and beer. [3] However, several wines and beers use strains of Brettanomyces in low concentrations in order to achieve the desired taste.

Brettanomyces claussenii is used for brewing beer. Originally isolated from strong English stock beer, it is a key component in some Belgian ales and sour beers . [4] It is said that fermentation with Brettanomyces claussenii will help a beer achieve English character.

An alternative theory is that Professor Claussen named Brettanomyces after his 'beloved' Brittany, not Britain. [5]

Taxonomy

Brettanomyces claussenii is a yeast in the kingdom fungi, the phylum ascomycota and the subphylum saccharomycotina, the so-called true yeasts. Yeasts in this subphylum reproduce asexually through budding. Baker's yeast and Brewer's yeast are in saccharomycotina as well.

The genus Dekkera can be used interchangeably with Brettanomyces when describing species; Dekkera are the teleomorphic (spore-forming) versions of the Brettanomyces species. Dekkera claussenii differs from other Dekkera species in its lack of blastesis and inability to ferment lactose. [6]

Examination of the Mitochondrial DNA of the genus Brettanomyces showed identical genomes in three pairs of species: Dekkera bruxellensis/Brettanomyces lambicus, Brettanomyces abstinens/Brettanomyces custerianus and Brettanomyces anomalus/Brettanomyces clausenii. It is suspected that the genus Dekkera has similar taxonomical redundancies. [7]

Uses

Brettanomyces claussenii is used for a variety of purposes. As a yeast, it is able to ferment cereals and fruits to produce beers and wines with unique flavor profiles. Brettanomyces claussenii can ferment a variety of other substances as well.

Ethanol fuel is becoming more prevalent as an alternative fuel source for automobiles. Using waste wood and agricultural residue is one way to produce ethanol without using crops that could potentially be food sources, such as corn. Combined with Pichia Stipitis R, Brettanomyces claussenii has been shown to produce ethanol from lignocellusic material. The material is prehydrolysed with sulfur dioxide and then simultaneously saccharified and fermented. This fermentation is rapid and efficient, producing between 360 and 370 liters of ethanol per ton of prehydrolysed lignocellulosic material. [8] However, Brettanomyces claussenii is unable to convert lactose to ethanol, like several other yeasts can. [9]

In clusters of Brettanomyces claussenii a negative pasteur effect has been demonstrated. The rate of alcohol fermentation is reduced under anaerobic conditions and stimulated by the presence atmospheric oxygen. The fermentation rate is sensitive to alkali concentrations. For example, a heavily potassium solution will increase the rate of fermentation while increasing sodium will slow the processes. [10]

See also

Related Research Articles

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Yeasts are eukaryotic, single-celled microorganisms classified as members of the fungus kingdom. The first yeast originated hundreds of millions of years ago, and at least 1,500 species are currently recognized. They are estimated to constitute 1% of all described fungal species.

<span class="mw-page-title-main">Lactic acid fermentation</span> Series of interconnected biochemical reactions

Lactic acid fermentation is a metabolic process by which glucose or other six-carbon sugars are converted into cellular energy and the metabolite lactate, which is lactic acid in solution. It is an anaerobic fermentation reaction that occurs in some bacteria and animal cells, such as muscle cells.

<span class="mw-page-title-main">Zymology</span> Study of fermentation and its uses

Zymology, also known as zymurgy, is an applied science that studies the biochemical process of fermentation and its practical uses. Common topics include the selection of fermenting yeast and bacteria species and their use in brewing, wine making, fermenting milk, and the making of other fermented foods.

<i>Brettanomyces</i> Genus of fungi

Brettanomyces is a non-spore forming genus of yeast in the family Saccharomycetaceae, and is often colloquially referred to as "Brett". The genus name Dekkera is used interchangeably with Brettanomyces, as it describes the teleomorph or spore forming form of the yeast, but is considered deprecated under the one fungus, one name change. The cellular morphology of the yeast can vary from ovoid to long "sausage" shaped cells. The yeast is acidogenic, and when grown on glucose rich media under aerobic conditions, produces large amounts of acetic acid. Brettanomyces is important to both the brewing and wine industries due to the sensory compounds it produces.

<span class="mw-page-title-main">Malolactic fermentation</span> Process in winemaking

Malolactic conversion is a process in winemaking in which tart-tasting malic acid, naturally present in grape must, is converted to softer-tasting lactic acid. Malolactic fermentation is most often performed as a secondary fermentation shortly after the end of the primary fermentation, but can sometimes run concurrently with it. The process is standard for most red wine production and common for some white grape varieties such as Chardonnay, where it can impart a "buttery" flavor from diacetyl, a byproduct of the reaction.

<span class="mw-page-title-main">Ethanol fermentation</span> Biological process that produces ethanol and carbon dioxide as by-products

Ethanol fermentation, also called alcoholic fermentation, is a biological process which converts sugars such as glucose, fructose, and sucrose into cellular energy, producing ethanol and carbon dioxide as by-products. Because yeasts perform this conversion in the absence of oxygen, alcoholic fermentation is considered an anaerobic process. It also takes place in some species of fish where it provides energy when oxygen is scarce.

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<i>Saccharomyces</i> Genus of fungi

Saccharomyces is a genus of fungi that includes many species of yeasts. Saccharomyces is from Greek σάκχαρον (sugar) and μύκης (fungus) and means sugar fungus. Many members of this genus are considered very important in food production where they are known as brewer's yeast, baker's yeast and sourdough starter among others. They are unicellular and saprotrophic fungi. One example is Saccharomyces cerevisiae, which is used in making bread, wine, and beer, and for human and animal health. Other members of this genus include the wild yeast Saccharomyces paradoxus that is the closest relative to S. cerevisiae, Saccharomyces bayanus, used in making wine, and Saccharomyces cerevisiaevar. boulardii, used in medicine.

<i>Brettanomyces bruxellensis</i> Species of fungus

Brettanomyces bruxellensis is a yeast associated with the Senne valley near Brussels, Belgium. Despite its Latin species name, B. bruxellensis is found all over the globe. In the wild, it is often found on the skins of fruit.

<span class="mw-page-title-main">Saccharomycotina</span> Subdivision of fungi

Saccharomycotina is a subdivision (subphylum) of the division (phylum) Ascomycota in the kingdom Fungi. It comprises most of the ascomycete yeasts. The members of Saccharomycotina reproduce by budding and they do not produce ascocarps.

<span class="mw-page-title-main">Fermentation</span> Metabolic process producing energy in the absence of oxygen

Fermentation is a metabolic process that produces chemical changes in organic substances through the action of enzymes. In biochemistry, fermentation is narrowly defined as the extraction of energy from carbohydrates in the absence of oxygen, while in food production, it may more broadly refer to any process in which the activity of microorganisms brings about a desirable change to a foodstuff or beverage. The science of fermentation is known as zymology.

<span class="mw-page-title-main">Fermentation in food processing</span> Converting carbohydrates to alcohol or acids using anaerobic microorganisms

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Symbiotic culture of bacteria and yeast (SCOBY) is a culinary symbiotic fermentation culture (starter) consisting of lactic acid bacteria (LAB), acetic acid bacteria (AAB), and yeast which arises in the preparation of sour foods and beverages such as kombucha. Beer and wine also undergo fermentation with yeast, but the lactic acid bacteria and acetic acid bacteria components unique to SCOBY are usually viewed as a source of spoilage rather than a desired addition. Both LAB and AAB enter on the surface of barley and malt in beer fermentation and grapes in wine fermentation; LAB lowers the pH of the beer/wine while AAB takes the ethanol produced from the yeast and oxidizes it further into vinegar, resulting in a sour taste and smell. AAB are also responsible for the formation of the cellulose SCOBY.

<span class="mw-page-title-main">Sour beer</span> Beer with a tart or sour taste

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<span class="mw-page-title-main">Yeast in winemaking</span> Yeasts used for alcoholic fermentation of wine

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References

  1. Gilliland, R. B. (1961). "Brettanomyces. I. Occurrence, Characteristics, and Effects on Beer Flavour". Journal of the Institute of Brewing. 67 (3): 257–261. doi: 10.1002/j.2050-0416.1961.tb01791.x .
  2. Jan Steensels; et al. (2015). "Brettanomyces yeasts – From spoilage organisms to valuable contributors to industrial fermentations". International Journal of Food Microbiology. 206: 24–38. doi: 10.1016/j.ijfoodmicro.2015.04.005 . PMID   25916511.
  3. "Oxford Companion to Wine – Brettanomyces". Also Heresztyn, T (1986). "Formation of substituted tetrahydropyridines by species of Brettanomyces and Lactobacillus isolated from mousey wines". American Journal of Enology and Viticulture (37): 127–32.
  4. Burningham, Lucy. The New York Times 1 June 2010. Web. Archived 26 Apr. 2011. Sour Beer Is Risky Business, Starting With the Name.
  5. Alexander, John. 'A Guide To Craft Brewing' page 170.
  6. Lee, Fwu-Wing, and Shung-Chang Jong. "Dekerra Claussenii; The Perfect State of Brettanomyces Claussenii." Mycotaxon 23 (1985): 275–78.
  7. McArthur, C. R., and G.D. Clark-Walker. "Mitochondrial DNA Size Diversity in the Dekkera/Brettanomyces Yeasts." Current Genetics 7 (1983): 29–35.
  8. Wayman, Morris, Rannade S. Parekh, and Sarad R. Parekh. "Simultaneous Saccharification and Fermentation by Mixed Cultures of Brettanomycesclausenii And Pichiaspipitis R of SO2-prehydrolysed Wood." Biotechnology Letters 9 (1987): 435–40.
  9. Bothast, R.J., C.P. Kurtzman, M.D. Saltarelli, and P.J. Slininger. "Ethanol Production by 107 Strains of Yeast on 5, 10 and 20% Lactose." Biotechnology Letters 8.8 (1986): 593–96.
  10. Wilken, T. O., A.J.M. Verhaar, and W. A. Scheffers. "The Influence of Potassium and Sodium Ions on the Negative Pasteur Effect in Brettanomyees Claussenii Clusters." Archiv für Mikrobiologie 42.2 (1962): 226–36.