CDC45 is a protein that in humans is encoded by the CDC45L gene. [5] [6] [7] [8]
The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein plays a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple polyadenylation sites of this gene are reported. [8]
CDC45-related protein has been shown to interact with:
A pre-replication complex (pre-RC) is a protein complex that forms at the origin of replication during the initiation step of DNA replication. Formation of the pre-RC is required for DNA replication to occur. Complete and faithful replication of the genome ensures that each daughter cell will carry the same genetic information as the parent cell. Accordingly, formation of the pre-RC is a very important part of the cell cycle.
DNA replication licensing factor MCM6 is a protein that in humans is encoded by the MCM6 gene. MCM6 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication.
Eukaryotic DNA replication is a conserved mechanism that restricts DNA replication to once per cell cycle. Eukaryotic DNA replication of chromosomal DNA is central for the duplication of a cell and is necessary for the maintenance of the eukaryotic genome.
The minichromosome maintenance protein complex (MCM) is a DNA helicase essential for genomic DNA replication. Eukaryotic MCM consists of six gene products, Mcm2–7, which form a heterohexamer. As a critical protein for cell division, MCM is also the target of various checkpoint pathways, such as the S-phase entry and S-phase arrest checkpoints. Both the loading and activation of MCM helicase are strictly regulated and are coupled to cell growth cycles. Deregulation of MCM function has been linked to genomic instability and a variety of carcinomas.
Replication protein A 70 kDa DNA-binding subunit is a protein that in humans is encoded by the RPA1 gene.
DNA replication licensing factor MCM7 is a protein that in humans is encoded by the MCM7 gene.
DNA replication licensing factor MCM2 is a protein that in humans is encoded by the MCM2 gene.
DNA replication licensing factor MCM3 is a protein that in humans is encoded by the MCM3 gene.
Cell division control protein 6 homolog is a protein that in humans is encoded by the CDC6 gene.
DNA replication licensing factor MCM4 is a protein that in humans is encoded by the MCM4 gene.
Origin recognition complex subunit 2 is a protein that is encoded by the ORC2 (ORC2L) gene in humans.
DNA replication licensing factor MCM5 is a protein that in humans is encoded by the MCM5 gene.
Cell division cycle 7-related protein kinase is an enzyme that in humans is encoded by the CDC7 gene. The Cdc7 kinase is involved in regulation of the cell cycle at the point of chromosomal DNA replication. The gene CDC7 appears to be conserved throughout eukaryotic evolution; this means that most eukaryotic cells have the Cdc7 kinase protein.
DNA repair protein REV1 is a protein that in humans is encoded by the REV1 gene.
Protein MCM10 homolog is a protein that in humans is encoded by the MCM10 gene. It is essential for activation of the Cdc45:Mcm2-7:GINS helicase, and thus required for proper DNA replication.
80 kDa MCM3-associated protein is a protein that in humans is encoded by the MCM3AP gene.
DNA replication licensing factor MCM8 is a protein that in humans is encoded by the MCM8 gene.
In cell biology, eukaryotes possess a regulatory system that ensures that DNA replication occurs only once per cell cycle.
DNA polymerase alpha subunit 2 is an enzyme that in humans is encoded by the POLA2 gene.
DNA polymerase alpha catalytic subunit is an enzyme that in humans is encoded by the POLA1 gene.