CPSF2

CPSF2
Identifiers
Aliases	CPSF2 , CPSF100, cleavage and polyadenylation specific factor 2
External IDs	OMIM: 606028 MGI: 1861601 HomoloGene: 6460 GeneCards: CPSF2
Gene location (Human)
Chr.	Chromosome 14 (human)
End	92,172,145 bp
Gene location (Mouse)
Chr.	Chromosome 12 (mouse)
End	101,972,683 bp
RNA expression pattern
	Top expressed in
	islet of Langerhans; ; stromal cell of endometrium; ; Achilles tendon; ; appendix; ; monocyte; ; corpus callosum; ; bone marrow cells; ; tibialis anterior muscle; ; internal globus pallidus; ; secondary oocyte;
	Top expressed in
	medial ganglionic eminence; ; secondary oocyte; ; cumulus cell; ; abdominal wall; ; lacrimal gland; ; primitive streak; ; pineal gland; ; epithelium of stomach; ; lens; ; ectoderm;
	More reference expression data
	; ;
	More reference expression data
Gene ontology
Molecular function	protein binding ; RNA binding ;
Cellular component	mRNA cleavage and polyadenylation specificity factor complex ; membrane ; nucleus ; nucleoplasm ;
Biological process	mRNA splicing, via spliceosome ; termination of RNA polymerase II transcription ; mRNA polyadenylation ; mRNA processing ; mRNA cleavage ; mRNA 3'-end processing by stem-loop binding and cleavage ; mRNA 3'-end processing ; mRNA export from nucleus ;
	Sources:Amigo / QuickGO
Orthologs
	53981
	51786
	ENSG00000165934
	ENSMUSG00000041781
	Q9P2I0
	O35218
	NM_017437 ; NM_001322270 ; NM_001322271 ; NM_001322272
	NM_016856
	NP_001309199 ; NP_001309200 ; NP_001309201 ; NP_059133
	NP_058552
	Wikidata
View/Edit Human	View/Edit Mouse

Last updated January 14, 2024

Cleavage and polyadenylation specificity factor subunit 2 is a protein that in humans is encoded by the CPSF2 gene.^[5]^[6]^[7] This protein is a subunit of the cleavage and polyadenylation specificity factor (CPSF) complex which plays a key role in pre-mRNA 3' end processing and polyadenylation.^[8] The CPSF2 protein connects the two subunits of the complex, mCF and mPSF.^[8] Its structure contributes both to the stability of the subunits interaction and to the flexibility of the complex necessary for function.^[9] This protein has been identified as an essential subunit of the complex as certain mutations in the region inhibit CPSF complex formation.^[9]

Related Research Articles

Polyadenylation is the addition of a poly(A) tail to an RNA transcript, typically a messenger RNA (mRNA). The poly(A) tail consists of multiple adenosine monophosphates; in other words, it is a stretch of RNA that has only adenine bases. In eukaryotes, polyadenylation is part of the process that produces mature mRNA for translation. In many bacteria, the poly(A) tail promotes degradation of the mRNA. It, therefore, forms part of the larger process of gene expression.

The TATA-binding protein (TBP) is a general transcription factor that binds specifically to a DNA sequence called the TATA box. This DNA sequence is found about 30 base pairs upstream of the transcription start site in some eukaryotic gene promoters.

Transcriptional modification or co-transcriptional modification is a set of biological processes common to most eukaryotic cells by which an RNA primary transcript is chemically altered following transcription from a gene to produce a mature, functional RNA molecule that can then leave the nucleus and perform any of a variety of different functions in the cell. There are many types of post-transcriptional modifications achieved through a diverse class of molecular mechanisms.

Cleavage and polyadenylation specificity factor (CPSF) is involved in the cleavage of the 3' signaling region from a newly synthesized pre-messenger RNA (pre-mRNA) molecule in the process of gene transcription. In eukaryotes, messenger RNA precursors (pre-mRNA) are transcribed in the nucleus from DNA by the enzyme, RNA polymerase II. The pre-mRNA must undergo post-transcriptional modifications, forming mature RNA (mRNA), before they can be transported into the cytoplasm for translation into proteins. The post-transcriptional modifications are: the addition of a 5' m7G cap, splicing of intronic sequences, and 3' cleavage and polyadenylation.

Eukaryotic transcription is the elaborate process that eukaryotic cells use to copy genetic information stored in DNA into units of transportable complementary RNA replica. Gene transcription occurs in both eukaryotic and prokaryotic cells. Unlike prokaryotic RNA polymerase that initiates the transcription of all different types of RNA, RNA polymerase in eukaryotes comes in three variations, each translating a different type of gene. A eukaryotic cell has a nucleus that separates the processes of transcription and translation. Eukaryotic transcription occurs within the nucleus where DNA is packaged into nucleosomes and higher order chromatin structures. The complexity of the eukaryotic genome necessitates a great variety and complexity of gene expression control.

Splicing factor 3 subunit 1 is a protein that in humans is encoded by the SF3A1 gene.

Heterogeneous nuclear ribonucleoprotein F is a protein that in humans is encoded by the HNRNPF gene.

Cleavage stimulation factor 64 kDa subunit is a protein that in humans is encoded by the CSTF2 gene.

Cleavage and polyadenylation specificity factor subunit 5 (CPSF5) is an enzyme that in humans is encoded by the NUDT21 gene. It belongs to the Nudix family of hydrolases.

Cleavage and polyadenylation specificity factor subunit 1 is a protein that in humans is encoded by the CPSF1 gene.

Poly(A) polymerase alpha is an enzyme that in humans is encoded by the PAPOLA gene.

Cleavage stimulation factor 50 kDa subunit is a protein that in humans is encoded by the CSTF1 gene.

Cleavage stimulation factor 77 kDa subunit is a protein that in humans is encoded by the CSTF3 gene.

Cleavage stimulation factor 64 kDa subunit, tau variant is a protein that in humans is encoded by the CSTF2T gene.

Cleavage and polyadenylation specificity factor subunit 3 is a protein that in humans is encoded by the CPSF3 gene.

Cleavage and polyadenylation specificity factor subunit 4 is a protein that in humans is encoded by the CPSF4 gene.

Cleavage and polyadenylation specificity factor subunit 6 is a protein that in humans is encoded by the CPSF6 gene.

Integrator complex subunit 11 is a protein that in humans is encoded by the CPSF3L gene.

Poly(A) polymerase gamma is an enzyme that in humans is encoded by the PAPOLG gene.

Cleavage and polyadenylation specificity factor subunit 7 is a protein that in humans is encoded by the CPSF7 gene.

References

1 2 3 GRCh38: Ensembl release 89: ENSG00000165934 - Ensembl, May 2017
1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000041781 - Ensembl, May 2017
↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ Jenny A, Hauri HP, Keller W (Dec 1994). "Characterization of cleavage and polyadenylation specificity factor and cloning of its 100-kilodalton subunit". Mol Cell Biol. 14 (12): 8183–90. doi:10.1128/MCB.14.12.8183. PMC 359357 . PMID 7969155.
↑ Samiotaki M, Balatsos NA, Courtis N, Tsiapalis CM (Jan 2001). "Assignment of the 100-kDa subunit of cleavage and polyadenylation specificity factor (CPSF2) to human chromosome 14q31.3 by radiation hybrid mapping". Cytogenet Cell Genet. 90 (3–4): 328–9. doi:10.1159/000056798. PMID 11124543. S2CID 84335742.
↑ "Entrez Gene: CPSF2 cleavage and polyadenylation specific factor 2, 100kDa".
1 2 "Human Gene CPSF2 (ENST00000298875.9) from GENCODE V43". genome.ucsc.edu. Retrieved 2023-03-05.
1 2 Zhang Y, Sun Y, Shi Y, Walz T, Tong L (2020-02-20). "Structural Insights into the Human Pre-mRNA 3′-End Processing Machinery". Molecular Cell. 77 (4): 800–809.e6. doi:10.1016/j.molcel.2019.11.005. ISSN 1097-2765. PMC 7036032 . PMID 31810758.

External links

Human CPSF2 genome location and CPSF2 gene details page in the UCSC Genome Browser .

Murthy KG, Manley JL (1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation". Genes Dev. 9 (21): 2672–83. doi: 10.1101/gad.9.21.2672 . PMID 7590244.
Thuresson AC, Aström J, Aström A, et al. (1994). "Multiple forms of poly(A) polymerases in human cells". Proc. Natl. Acad. Sci. U.S.A. 91 (3): 979–83. Bibcode:1994PNAS...91..979T. doi: 10.1073/pnas.91.3.979 . PMC 521437 . PMID 8302877.
Bienroth S, Keller W, Wahle E (1993). "Assembly of a processive messenger RNA polyadenylation complex". EMBO J. 12 (2): 585–94. doi:10.1002/j.1460-2075.1993.tb05690.x. PMC 413241 . PMID 8440247.
Schul W, Groenhout B, Koberna K, et al. (1996). "The RNA 3' cleavage factors CstF 64 kDa and CPSF 100 kDa are concentrated in nuclear domains closely associated with coiled bodies and newly synthesized RNA". EMBO J. 15 (11): 2883–92. doi:10.1002/j.1460-2075.1996.tb00649.x. PMC 450226 . PMID 8654386.
McCracken S, Fong N, Yankulov K, et al. (1997). "The C-terminal domain of RNA polymerase II couples mRNA processing to transcription". Nature. 385 (6614): 357–61. Bibcode:1997Natur.385..357M. doi:10.1038/385357a0. PMID 9002523. S2CID 4307073.
Takagaki Y, Manley JL (2000). "Complex protein interactions within the human polyadenylation machinery identify a novel component". Mol. Cell. Biol. 20 (5): 1515–25. doi:10.1128/MCB.20.5.1515-1525.2000. PMC 85326 . PMID 10669729.
Nagase T, Kikuno R, Ishikawa KI, et al. (2000). "Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 7 (1): 65–73. doi: 10.1093/dnares/7.1.65 . PMID 10718198.
Monsalve M, Wu Z, Adelmant G, et al. (2000). "Direct coupling of transcription and mRNA processing through the thermogenic coactivator PGC-1". Mol. Cell. 6 (2): 307–16. doi: 10.1016/S1097-2765(00)00031-9 . PMID 10983978.
de Vries H, Rüegsegger U, Hübner W, et al. (2000). "Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors". EMBO J. 19 (21): 5895–904. doi:10.1093/emboj/19.21.5895. PMC 305781 . PMID 11060040.
Hodgman R, Tay J, Mendez R, Richter JD (2001). "CPEB phosphorylation and cytoplasmic polyadenylation are catalyzed by the kinase IAK1/Eg2 in maturing mouse oocytes". Development. 128 (14): 2815–22. doi:10.1242/dev.128.14.2815. PMID 11526086.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC 139241 . PMID 12477932.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi: 10.1038/ng1285 . PMID 14702039.
Kaufmann I, Martin G, Friedlein A, et al. (2005). "Human Fip1 is a subunit of CPSF that binds to U-rich RNA elements and stimulates poly(A) polymerase". EMBO J. 23 (3): 616–26. doi:10.1038/sj.emboj.7600070. PMC 1271804 . PMID 14749727.
Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. Bibcode:2004PNAS..10112130B. doi: 10.1073/pnas.0404720101 . PMC 514446 . PMID 15302935.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928 . PMID 15489334.
Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129 . PMID 16344560.
Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi: 10.1016/j.cell.2006.09.026 . PMID 17081983.
Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948 . PMID 17353931.

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[refGRCh38Ensembl-1] 1 2 3 GRCh38: Ensembl release 89: ENSG00000165934 - Ensembl, May 2017

[refGRCm38Ensembl-2] 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000041781 - Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid7969155-5] Jenny A, Hauri HP, Keller W (Dec 1994). "Characterization of cleavage and polyadenylation specificity factor and cloning of its 100-kilodalton subunit". Mol Cell Biol. 14 (12): 8183–90. doi:10.1128/MCB.14.12.8183. PMC 359357 . PMID 7969155.

[pmid11124543-6] Samiotaki M, Balatsos NA, Courtis N, Tsiapalis CM (Jan 2001). "Assignment of the 100-kDa subunit of cleavage and polyadenylation specificity factor (CPSF2) to human chromosome 14q31.3 by radiation hybrid mapping". Cytogenet Cell Genet. 90 (3–4): 328–9. doi:10.1159/000056798. PMID 11124543. S2CID 84335742.

[entrez-7] "Entrez Gene: CPSF2 cleavage and polyadenylation specific factor 2, 100kDa".

[:0-8] 1 2 "Human Gene CPSF2 (ENST00000298875.9) from GENCODE V43". genome.ucsc.edu. Retrieved 2023-03-05.

[:1-9] 1 2 Zhang Y, Sun Y, Shi Y, Walz T, Tong L (2020-02-20). "Structural Insights into the Human Pre-mRNA 3′-End Processing Machinery". Molecular Cell. 77 (4): 800–809.e6. doi:10.1016/j.molcel.2019.11.005. ISSN 1097-2765. PMC 7036032 . PMID 31810758.

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CPSF2

Contents

Related Research Articles

References

External links

Further reading