CPSF6

CPSF6
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	3P5T, 3P6Y, 3Q2S, 3Q2T, 4B4N, 4U0A, 4U0B, 4WYM
Identifiers
Aliases	CPSF6 , CFIM, CFIM68, HPBRII-4, HPBRII-7, cleavage and polyadenylation specific factor 6, CFIM72
External IDs	OMIM: 604979 MGI: 1913948 HomoloGene: 134126 GeneCards: CPSF6
Gene location (Human)
Chr.	Chromosome 12 (human)
End	69,274,358 bp
Gene location (Mouse)
Chr.	Chromosome 10 (mouse)
End	117,215,920 bp
RNA expression pattern
	Top expressed in
	ganglionic eminence; ; bone marrow cells; ; Achilles tendon; ; thymus; ; appendix; ; sural nerve; ; endometrium; ; islet of Langerhans; ; lymph node; ; stromal cell of endometrium;
	Top expressed in
	superior cervical ganglion; ; hand; ; otolith organ; ; utricle; ; cumulus cell; ; trigeminal ganglion; ; maxillary prominence; ; abdominal wall; ; ganglionic eminence; ; vas deferens;
	More reference expression data
	; ;
	More reference expression data
Gene ontology
Molecular function	protein binding ; mRNA binding ; RNA binding ; nucleic acid binding ; ribosomal large subunit binding ; exon-exon junction complex binding ;
Cellular component	paraspeckles ; membrane ; nucleus ; nucleoplasm ; nuclear speck ; mRNA cleavage factor complex ; perichromatin fibrils ; cytoplasm ; mRNA cleavage and polyadenylation specificity factor complex ; interchromatin granule ; ribonucleoprotein complex ;
Biological process	mRNA polyadenylation ; mRNA processing ; protein tetramerization ; positive regulation of RNA export from nucleus ; protein heterotetramerization ; pre-mRNA cleavage required for polyadenylation ; mRNA alternative polyadenylation ; messenger ribonucleoprotein complex assembly ;
	Sources:Amigo / QuickGO
Orthologs
	11052
	432508
	ENSG00000111605
	ENSMUSG00000055531
	Q16630
	Q6NVF9
	NM_001300947 ; NM_007007
	NM_001013391 ; NM_001310609
	NP_001287876 ; NP_008938
	NP_001013409 ; NP_001297538
	Wikidata
View/Edit Human	View/Edit Mouse

Last updated December 12, 2023

Cleavage and polyadenylation specificity factor subunit 6 is a protein that in humans is encoded by the CPSF6 gene.^[5]^[6]^[7]

Function

The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. The cleavage factor complex is composed of four polypeptides. This gene encodes the 68kD subunit. It has a domain organization reminiscent of spliceosomal proteins.^[7]

Interactions

CPSF6 has been shown to interact with WWP1 ^[8] and PLSCR1.^[8]

CPSF6 plays an important role in the nuclear import and integration of HIV-1 capsids.^[9]

Related Research Articles

Polyadenylation is the addition of a poly(A) tail to an RNA transcript, typically a messenger RNA (mRNA). The poly(A) tail consists of multiple adenosine monophosphates; in other words, it is a stretch of RNA that has only adenine bases. In eukaryotes, polyadenylation is part of the process that produces mature mRNA for translation. In many bacteria, the poly(A) tail promotes degradation of the mRNA. It, therefore, forms part of the larger process of gene expression.

Cleavage and polyadenylation specificity factor (CPSF) is involved in the cleavage of the 3' signaling region from a newly synthesized pre-messenger RNA (pre-mRNA) molecule in the process of gene transcription. In eukaryotes, messenger RNA precursors (pre-mRNA) are transcribed in the nucleus from DNA by the enzyme, RNA polymerase II. The pre-mRNA must undergo post-transcriptional modifications, forming mature RNA (mRNA), before they can be transported into the cytoplasm for translation into proteins. The post-transcriptional modifications are: the addition of a 5' m7G cap, splicing of intronic sequences, and 3' cleavage and polyadenylation.

Splicing factor U2AF 65 kDa subunit is a protein that in humans is encoded by the U2AF2 gene.

Splicing factor U2AF 35 kDa subunit is a protein that in humans is encoded by the U2AF1 gene.

Polyadenylate-binding protein 2 (PABP-2) also known as polyadenylate-binding nuclear protein 1 (PABPN1) is a protein that in humans is encoded by the PABPN1 gene. PABN1 is a member of a larger family of poly(A)-binding proteins in the human genome.

Cleavage stimulation factor 64 kDa subunit is a protein that in humans is encoded by the CSTF2 gene.

Serine/arginine-rich splicing factor 7 (SRSF7) also known as splicing factor, arginine/serine-rich 7 (SFRS7) or splicing factor 9G8 is a protein that in humans is encoded by the SRSF7 gene.

Cleavage and polyadenylation specificity factor subunit 5 (CPSF5) is an enzyme that in humans is encoded by the NUDT21 gene. It belongs to the Nudix family of hydrolases.

Eukaryotic translation initiation factor 3 subunit I (eIF3i) is a protein that in humans is encoded by the EIF3I gene.

Cleavage and polyadenylation specificity factor subunit 2 is a protein that in humans is encoded by the CPSF2 gene. This protein is a subunit of the cleavage and polyadenylation specificity factor (CPSF) complex which plays a key role in pre-mRNA 3' end processing and polyadenylation. The CPSF2 protein connects the two subunits of the complex, mCF and mPSF. Its structure contributes both to the stability of the subunits interaction and to the flexibility of the complex necessary for function. This protein has been identified as an essential subunit of the complex as certain mutations in the region inhibit CPSF complex formation.

Cleavage and polyadenylation specificity factor subunit 1 is a protein that in humans is encoded by the CPSF1 gene.

DNA-directed RNA polymerases I and III subunit RPAC2 is a protein that in humans is encoded by the POLR1D gene.

Poly(A) polymerase alpha is an enzyme that in humans is encoded by the PAPOLA gene.

Cleavage stimulation factor 50 kDa subunit is a protein that in humans is encoded by the CSTF1 gene.

Cleavage stimulation factor 77 kDa subunit is a protein that in humans is encoded by the CSTF3 gene.

Cleavage stimulation factor 64 kDa subunit, tau variant is a protein that in humans is encoded by the CSTF2T gene.

Cleavage and polyadenylation specificity factor subunit 3 is a protein that in humans is encoded by the CPSF3 gene.

Cleavage and polyadenylation specificity factor subunit 4 is a protein that in humans is encoded by the CPSF4 gene.

Caprin-1 is a protein that in humans is encoded by the CAPRIN1 gene. It is suggested that Caprin1 is essential for the formation of long-term memory.

Cleavage and polyadenylation specificity factor subunit 7 is a protein that in humans is encoded by the CPSF7 gene.

References

1 2 3 GRCh38: Ensembl release 89: ENSG00000111605 - Ensembl, May 2017
1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000055531 - Ensembl, May 2017
↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ Rüegsegger U, Blank D, Keller W (Jan 1998). "Human pre-mRNA cleavage factor Im is related to spliceosomal SR proteins and can be reconstituted in vitro from recombinant subunits". Molecular Cell. 1 (2): 243–53. doi: 10.1016/S1097-2765(00)80025-8 . PMID 9659921.
↑ Cardinale S, Cisterna B, Bonetti P, Aringhieri C, Biggiogera M, Barabino SM (Apr 2007). "Subnuclear localization and dynamics of the Pre-mRNA 3' end processing factor mammalian cleavage factor I 68-kDa subunit". Molecular Biology of the Cell. 18 (4): 1282–92. doi:10.1091/mbc.E06-09-0846. PMC 1838998 . PMID 17267687.
1 2 "Entrez Gene: CPSF6 cleavage and polyadenylation specific factor 6, 68kDa".
1 2 Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, et al. (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
↑ Müller, Thorsten G.; Zila, Vojtech; Müller, Barbara; Kräusslich, Hans-Georg (29 September 2022). "Nuclear Capsid Uncoating and Reverse Transcription of HIV-1". Annual Review of Virology. 9 (1): 261–284. doi: 10.1146/annurev-virology-020922-110929 .

External links

Human CPSF6 genome location and CPSF6 gene details page in the UCSC Genome Browser .

Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Rüegsegger U, Beyer K, Keller W (Mar 1996). "Purification and characterization of human cleavage factor Im involved in the 3' end processing of messenger RNA precursors". The Journal of Biological Chemistry. 271 (11): 6107–13. doi: 10.1074/jbc.271.11.6107 . PMID 8626397.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
de Vries H, Rüegsegger U, Hübner W, Friedlein A, Langen H, Keller W (Nov 2000). "Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors". The EMBO Journal. 19 (21): 5895–904. doi:10.1093/emboj/19.21.5895. PMC 305781 . PMID 11060040.
Awasthi S, Alwine JC (Nov 2003). "Association of polyadenylation cleavage factor I with U1 snRNP". RNA. 9 (11): 1400–9. doi:10.1261/rna.5104603. PMC 1287061 . PMID 14561889.
Brown KM, Gilmartin GM (Dec 2003). "A mechanism for the regulation of pre-mRNA 3' processing by human cleavage factor Im". Molecular Cell. 12 (6): 1467–76. doi: 10.1016/S1097-2765(03)00453-2 . PMID 14690600.
Dettwiler S, Aringhieri C, Cardinale S, Keller W, Barabino SM (Aug 2004). "Distinct sequence motifs within the 68-kDa subunit of cleavage factor Im mediate RNA binding, protein-protein interactions, and subcellular localization" (PDF). The Journal of Biological Chemistry. 279 (34): 35788–97. doi: 10.1074/jbc.M403927200 . PMID 15169763. S2CID 39752996.
Venkataraman K, Brown KM, Gilmartin GM (Jun 2005). "Analysis of a noncanonical poly(A) site reveals a tripartite mechanism for vertebrate poly(A) site recognition". Genes & Development. 19 (11): 1315–27. doi:10.1101/gad.1298605. PMC 1142555 . PMID 15937220.
Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
Millevoi S, Loulergue C, Dettwiler S, Karaa SZ, Keller W, Antoniou M, Vagner S (Oct 2006). "An interaction between U2AF 65 and CF I(m) links the splicing and 3' end processing machineries". The EMBO Journal. 25 (20): 4854–64. doi:10.1038/sj.emboj.7601331. PMC 1618107 . PMID 17024186.
Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948 . PMID 17353931.

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[refGRCh38Ensembl-1] 1 2 3 GRCh38: Ensembl release 89: ENSG00000111605 - Ensembl, May 2017

[refGRCm38Ensembl-2] 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000055531 - Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid9659921-5] Rüegsegger U, Blank D, Keller W (Jan 1998). "Human pre-mRNA cleavage factor Im is related to spliceosomal SR proteins and can be reconstituted in vitro from recombinant subunits". Molecular Cell. 1 (2): 243–53. doi: 10.1016/S1097-2765(00)80025-8 . PMID 9659921.

[pmid17267687-6] Cardinale S, Cisterna B, Bonetti P, Aringhieri C, Biggiogera M, Barabino SM (Apr 2007). "Subnuclear localization and dynamics of the Pre-mRNA 3' end processing factor mammalian cleavage factor I 68-kDa subunit". Molecular Biology of the Cell. 18 (4): 1282–92. doi:10.1091/mbc.E06-09-0846. PMC 1838998 . PMID 17267687.

[entrez-7] 1 2 "Entrez Gene: CPSF6 cleavage and polyadenylation specific factor 6, 68kDa".

[pmid16189514-8] 1 2 Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, et al. (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.

[9] Müller, Thorsten G.; Zila, Vojtech; Müller, Barbara; Kräusslich, Hans-Georg (29 September 2022). "Nuclear Capsid Uncoating and Reverse Transcription of HIV-1". Annual Review of Virology. 9 (1): 261–284. doi: 10.1146/annurev-virology-020922-110929 .

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