| Candida oleophila | |
|---|---|
Scientific classification  | |
| Domain: | Eukaryota |
| Kingdom: | Fungi |
| Division: | Ascomycota |
| Class: | Saccharomycetes |
| Order: | Saccharomycetales |
| Family: | Saccharomycetaceae |
| Genus: | Candida |
| Species: | C. oleophila |
| Binomial name | |
| Candida oleophila Montrocher, 1967 [1] | |
| Synonyms | |
Yarrowia lipolytica | |
Candida oleophila is a species of yeast in the genus Candida in the family of Saccharomycetaceae. It is used in post-harvesting fruit and vegetables as an alternative for fungicides.
Candida oleophila was described by Montrocher in 1967 as the family of Dipodascaceae; in the same year, it was described by Kaisha & Iizuka as a family of Saccharomycetales.
Candida oleophila is a yeast, which is part of Aspire, a product that is used in commercial settings, and is recommended to control postharvest decay of fruit and vegetables. A species of yeast in the genus Candida has a hairpin structure shaped like a dumbbell called SMRTbell. One of the main modes of action is competition for nutrients and space. Also, a major role in the mechanism of action by yeast antagonists is the degradation of enzymes that degrade the fungal wall.
This yeast is commonly found in plants and debris, which are the main natural habitat for most yeast species. Candida oleophila Strain O is a single-celled yeast found naturally on plant tissues (fruits, flowers, and wood) and in water. The exudations, better known as plant secretions, contain sugars and other compounds that help the nutrition of yeasts, better known as epiphytes.
According to Siobhán A. Turner and Geraldine Butler, in conjunction with L Mikhailova of the Department of Morphology of Microorganisms and Electron Microscopy Institute of Microbiology, Bulgarian Academy of Sciences says: Candida olephila shows complex bioactive compounds, which are the primary basis of the benefit of the fruits after the post-harvest. Penicillium cell wall fragments and glucose stimulate the production of these three compounds. One main study on Candida oleophila showed that it could produce and secrete several cell wall-degrading enzymes; this includes compounds like exo-β-1,3-glucanase, protease, and chitinase. Exo-β-1,3-glucanase and chitinase could be produced in the early stages of growth, followed by protease reaching growth in a range of a week, approximately 6 to 8 days. This demonstrated that Candida oleophila could secrete exo-β-1,3-glucanase(CoEXG1) on the wound site of the fruit that was the subject of study for this experiment. These studies were also made based on in vitro in which they showed a proliferation of compounds made to fruits to test how the biocontrol activity on pathogen infection worked in a controlled setting.
Candida oleophila is found everywhere where biocontrol agents are needed to control post-harvest diseases of fruits and vegetables. Studies on prolonging the life of postharvest fruits and vegetables with Candida oleophila have concluded that biocontrol with C. oleophila can be used over fungicides. The fungicides used to maintain food control in agricultural areas, such as fruits and vegetables, are widely used, but a person is exposed for any reason, it can irritate the eyes and skin and cause harm if ingested.
Candida oleophila has previously been used in the laboratory. Together with the active agent I-182 from Candida oleophila, it was engineered as an active agent for commercial products such as Aspire. It is used for the central postharvest handling inside fruits, such as apples and pears, to control the growth of pathogenic fungi, such as the gray mold ( Botrytis cinerea ) and blue mold ( Penicillium expansum ). This helps to avoid losses in postharvest supplies, which leads to monetary losses for farmers.
How the growth of Candida oleophila in wound samples can be characterized is reflected in the ability to compete against pathogens that could damage the nutrition and space of the fruit or vegetable. Candida oleophila showed a rapid expansion observed on the third day of healing in the fruit or vegetable. Candida oleophila could show slow growth for approximately 3 to 7 days. The number of Candida oleophila yeast cells could reach a maximum growth average of 2.2 × 1011 CFU mL−1, which was 20.3 times higher than on day 0; later, the number of yeast decrease quickly. In the end, Candida oleophila reached a 5.3 × 107 CFU mL−1 on day 28, 1/18 of the amount present on day 0. C. oleophilia can colonize wounds and multiply rapidly on the surface of the injury, healing the tissue. At the same time, with rapid growth, losses and infections that could damage another postharvest can be avoided, reducing economic losses.
Chitinases are hydrolytic enzymes that break down glycosidic bonds in chitin. They catalyse the following reaction:
Ascomycota is a phylum of the kingdom Fungi that, together with the Basidiomycota, forms the subkingdom Dikarya. Its members are commonly known as the sac fungi or ascomycetes. It is the largest phylum of Fungi, with over 64,000 species. The defining feature of this fungal group is the "ascus", a microscopic sexual structure in which nonmotile spores, called ascospores, are formed. However, some species of Ascomycota are asexual and thus do not form asci or ascospores. Familiar examples of sac fungi include morels, truffles, brewers' and bakers' yeast, dead man's fingers, and cup fungi. The fungal symbionts in the majority of lichens such as Cladonia belong to the Ascomycota.
Botrytis cinerea is a necrotrophic fungus that affects many plant species, although its most notable hosts may be wine grapes. In viticulture, it is commonly known as "botrytis bunch rot"; in horticulture, it is usually called "grey mould" or "gray mold".
Candex is a dietary supplement manufactured by Pure Essence Laboratories. It is marketed as an enzymatic remedy to treat the yeast infection candida. Having the status of a dietary supplement, its efficiency has not been proven in scientifically controlled and peer-reviewed trials. Similar formulas exist, such as Candigest.
Echinocandins are a class of antifungal drugs that inhibit the synthesis of β-glucan in the fungal cell wall via noncompetitive inhibition of the enzyme 1,3-β glucan synthase. The class has been termed the "penicillin of antifungals," along with the related papulacandins, as their mechanism of action resembles that of penicillin in bacteria. β-glucans are carbohydrate polymers that are cross-linked with other fungal cell wall components, the fungal equivalent to bacterial peptidoglycan. Caspofungin, micafungin, and anidulafungin are semisynthetic echinocandin derivatives with limited clinical use due to their solubility, antifungal spectrum, and pharmacokinetic properties.
Penicillium expansum is a psychrophilic blue mold that is common throughout the world in soil. It causes Blue Mold of apples, one of the most prevalent and economically damaging post-harvest diseases of apples.
Penicillium funiculosum is a plant pathogen infecting pineapples.
Chaetomium cupreum is a fungus in the family Chaetomiaceae. It is able to decay in manufactured cellulosic materials, and is known to antagonize a wide range of soil microorganisms. This species is component of the biocontrol agent, Ketomium, a commercial biofungicide. It has also been investigated for use in the production of natural dyes. Chaetomium cupreum is mesophilic and known to occur in harsh environments and can rapidly colonize organic substrates in soil. Laboratory cultures of C. cupreum can be propagated on a range of common growth media including potato dextrose at ambient or higher than ambient temperature producing cottony white colonies with a reddish reverse.
Glucanases are enzymes that break down large polysaccharides via hydrolysis. The product of the hydrolysis reaction is called a glucan, a linear polysaccharide made of up to 1200 glucose monomers, held together with glycosidic bonds. Glucans are abundant in the endosperm cell walls of cereals such as barley, rye, sorghum, rice, and wheat. Glucanases are also referred to as lichenases, hydrolases, glycosidases, glycosyl hydrolases, and/or laminarinases. Many types of glucanases share similar amino acid sequences but vastly different substrates. Of the known endo-glucanases, 1,3-1,4-β-glucanase is considered the most active.
The role of yeast in winemaking is the most important element that distinguishes wine from fruit juice. In the absence of oxygen, yeast converts the sugars of the fruit into alcohol and carbon dioxide through the process of fermentation. The more sugars in the grapes, the higher the potential alcohol level of the wine if the yeast are allowed to carry out fermentation to dryness. Sometimes winemakers will stop fermentation early in order to leave some residual sugars and sweetness in the wine such as with dessert wines. This can be achieved by dropping fermentation temperatures to the point where the yeast are inactive, sterile filtering the wine to remove the yeast or fortification with brandy or neutral spirits to kill off the yeast cells. If fermentation is unintentionally stopped, such as when the yeasts become exhausted of available nutrients and the wine has not yet reached dryness, this is considered a stuck fermentation.
Raspberry spur blight is caused by the fungus Didymella applanata. This plant pathogen is more problematic on red raspberries (Rubus idaeus) than on black or purple raspberries. The fungus infects the leaves first and then spreads to the cane. It causes necrotic spots on the cane near the base of the petiole attachment. Raspberry spur blight can cause a significant reduction in yield, fruit blight, premature leaf drop, and weak bud and cane growth. The magnitude of damage is not clearly understood in the United States, however, studies from Scotland suggest damage to the cane itself is limited. The disease has minor economic impacts by reducing leaves in the summer or killing buds. Major economic damage occurs if the disease manages to kill the entire cane. In the United States, this disease is found in Oregon and Washington.
Previously classified under the species complex Aureobasidium pullulans, Aureobasidium subglaciale is a black yeast-like, extremophile, ascomycete fungus that is found in extreme cold habitats. The species was originally isolated from subglacial ice of arctic glaciers. The first isolate of this species was obtained from subglacial ice of the Norwegian island Spitsbergen, one of the coldest places inhabited by humans. of Genomic data collected from specimens in the Aureobasidium pullulans complex justified distinction of four different species
Penicillium oxalicum is an anamorph species of the genus Penicillium which was isolated from rhizosphere soil of pearl millet. Penicillium oxalicum produces secalonic acid D, chitinase, oxalic acid, oxaline and β-N-acetylglucosaminidase and occurs widespread in food and tropical commodities. This fungus could be used against soilborne diseases like downy mildew of tomatoes
Fludioxonil is a synthetic phenylpyrrole chemical introduced by Ciba-Geigy in 1993 for use as a non-systemic fungicide. It is a structural analog of the natural fungicide pyrrolnitrin.
Penicillium digitatum is a mesophilic fungus found in the soil of citrus-producing areas. It is a major source of post-harvest decay in fruits and is responsible for the widespread post-harvest disease in Citrus fruit known as green rot or green mould. In nature, this necrotrophic wound pathogen grows in filaments and reproduces asexually through the production of conidiophores and conidia. However, P. digitatum can also be cultivated in the laboratory setting. Alongside its pathogenic life cycle, P. digitatum is also involved in other human, animal and plant interactions and is currently being used in the production of immunologically based mycological detection assays for the food industry.
Metschnikowia pulcherrima is a ubiquitous species of yeast, with numerous strains, belonging to the family Metschnikowiaceae, and found on grapes, cherries, flowers, spoiled fruit and consequently carried by fruit flies. It is a non-Saccharomyces yeast and plays an important role in the vinification of wine when it is present on grapes or winery equipment, and has historically seen use in South Africa’s wine industry. It is also being studied at the University of Bath as a possible alternative to the use of Palm oil, and early results show promise. M. pulcherrima is ovoid to ellipsoidal in shape and reproduces by budding. Its cells are globose and thick-walled, holding a single, large oil droplet of high refractive index. As the result of incomplete budding where cells remain attached after division, pseudohyphae may form under anaerobic conditions.
Cellulosimicrobium cellulans is a Gram-positive bacterium from the genus of Cellulosimicrobium. Cellulosimicrobium cellulans can cause rare opportunistic infections. The strain EB-8-4 of this species can be used for stereoselective allylic hydroxylation of D-limonene to (+)-trans-carveol.
Rhodotorula glutinis is the type species of the genus Rhodotorula, a basidiomycetous genus of pink yeasts which contains 370 species. Heterogeneity of the genus has made its classification difficult with five varieties having been recognized; however, as of 2011, all are considered to represent a single taxon. The fungus is a common colonist of animals, foods and environmental materials. It can cause opportunistic infections, notably blood infection in the setting of significant underlying disease. It has been used industrially in the production of carotenoid pigments and as a biocontrol agent for post-harvest spoilage diseases of fruits.
Zymolyase is an enzyme mixture used to degrade the cell wall of yeast and form spheroplasts. Essential activities of zymolyase include β-1,3-glucan laminaripentao-hydrolase activity and β-1,3-glucanase activity. A common source of zymolyase is the Actinobacteria Arthrobacter luteus. Commercial sources of zymolyase may have some residual protease activity.
Ibrexafungerp, sold under the brand name Brexafemme, is an antifungal medication used to treat vulvovaginal candidiasis (VVC). It is taken orally. It is also currently undergoing clinical trials for other indications via an intravenous (IV) formulation. An estimated 75% of women will have at least one episode of VVC and 40 to 45% will have two or more episodes in their lifetime.
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