EFTUD2

EFTUD2
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	3JCR
Identifiers
Aliases	EFTUD2 , MFDGA, MFDM, SNRNP116, Snrp116, Snu114, U5-116KD, elongation factor Tu GTP binding domain containing 2
External IDs	OMIM: 603892 MGI: 1336880 HomoloGene: 3133 GeneCards: EFTUD2
Gene location (Human)
Chr.	Chromosome 17 (human)
End	44,899,445 bp
Gene location (Mouse)
Chr.	Chromosome 11 (mouse)
End	102,771,811 bp
RNA expression pattern
	Top expressed in
	bone marrow cells; ; tibialis anterior muscle; ; monocyte; ; ganglionic eminence; ; gastrocnemius muscle; ; stromal cell of endometrium; ; islet of Langerhans; ; Achilles tendon; ; appendix; ; sural nerve;
	Top expressed in
	maxillary prominence; ; abdominal wall; ; somite; ; dermis; ; internal carotid artery; ; external carotid artery; ; primitive streak; ; spermatocyte; ; foot; ; endocardial cushion;
	More reference expression data
	n/a
Gene ontology
Molecular function	nucleotide binding ; GTP binding ; protein binding ; GTPase activity ; RNA binding ; U5 snRNA binding ;
Cellular component	Cajal body ; nuclear speck ; catalytic step 2 spliceosome ; spliceosomal complex ; membrane ; nucleus ; nucleoplasm ; extracellular matrix ; cytosol ; U4/U6 x U5 tri-snRNP complex ; U2-type catalytic step 2 spliceosome ; ribonucleoprotein complex ; U2-type precatalytic spliceosome ;
Biological process	mRNA splicing, via spliceosome ; mRNA processing ; RNA splicing ; response to cocaine ;
	Sources:Amigo / QuickGO
Orthologs
	9343
	20624
	ENSG00000108883
	ENSMUSG00000020929
	Q15029
	O08810
	NM_001142605 ; NM_001258353 ; NM_001258354 ; NM_004247
	NM_001109995 ; NM_011431
	NP_001136077 ; NP_001245282 ; NP_001245283 ; NP_004238
	NP_001103465 ; NP_035561
	Wikidata
View/Edit Human	View/Edit Mouse

Last updated January 14, 2024

116 kDa U5 small nuclear ribonucleoprotein component is a protein that in humans is encoded by the EFTUD2 gene.^[5]^[6]

Disease associations

Heterozygous loss-of-function mutations in EFTUD2 cause Mandibulofacial Dysostosis with Microcephaly (MFDM; OMIM #610536),^[7] a multiple malformation syndrome comprising progressive microcephaly (present in all affected individuals), craniofacial skeletal anomalies, cleft palate, deafness, choanal atresia, small stature, and/or cardiac and thumb anomalies.

Interactions

EFTUD2 has been shown to interact with WDR57 ^[8]^[9] and PRPF8.^[9]

Related Research Articles

A spliceosome is a large ribonucleoprotein (RNP) complex found primarily within the nucleus of eukaryotic cells. The spliceosome is assembled from small nuclear RNAs (snRNA) and numerous proteins. Small nuclear RNA (snRNA) molecules bind to specific proteins to form a small nuclear ribonucleoprotein complex, which in turn combines with other snRNPs to form a large ribonucleoprotein complex called a spliceosome. The spliceosome removes introns from a transcribed pre-mRNA, a type of primary transcript. This process is generally referred to as splicing. An analogy is a film editor, who selectively cuts out irrelevant or incorrect material from the initial film and sends the cleaned-up version to the director for the final cut.

snRNPs, or small nuclear ribonucleoproteins, are RNA-protein complexes that combine with unmodified pre-mRNA and various other proteins to form a spliceosome, a large RNA-protein molecular complex upon which splicing of pre-mRNA occurs. The action of snRNPs is essential to the removal of introns from pre-mRNA, a critical aspect of post-transcriptional modification of RNA, occurring only in the nucleus of eukaryotic cells. Additionally, U7 snRNP is not involved in splicing at all, as U7 snRNP is responsible for processing the 3′ stem-loop of histone pre-mRNA.

5′-3′ exoribonuclease 1 (Xrn1) is a protein that in humans is encoded by the XRN1 gene. Xrn1 hydrolyses RNA in the 5′ to 3′ direction.

Pre-mRNA-processing-splicing factor 8 is a protein that in humans is encoded by the PRPF8 gene.

Small nuclear ribonucleoprotein Sm D1 is a protein that in humans is encoded by the SNRPD1 gene.

Splicing factor, proline- and glutamine-rich is a protein that in humans is encoded by the SFPQ gene.

PRP31 pre-mRNA processing factor 31 homolog , also known as PRPF31, is a protein which in humans is encoded by the PRPF31 gene.

U4/U6 small nuclear ribonucleoprotein Prp3 is a protein that in humans is encoded by the PRPF3 gene.

Splicing factor 3B subunit 1 is a protein that in humans is encoded by the SF3B1 gene.

U5 small nuclear ribonucleoprotein 200 kDa helicase is an enzyme that in humans is encoded by the SNRNP200 gene.

Splicing factor 3A subunit 2 is a protein that in humans is encoded by the SF3A2 gene.

Splicing factor 3B subunit 2 is a protein that in humans is encoded by the SF3B2 gene.

Splicing factor 3B subunit 4 is a protein that in humans is encoded by the SF3B4 gene.

Pre-mRNA-processing factor 6 is a protein that in humans is encoded by the PRPF6 gene.

NHP2-like protein 1 is a protein that in humans is encoded by the SNU13 gene.

Splicing factor 3B, 14 kDa subunit, also known as SF3B14, is a human gene.

WD repeat domain 57 , also known as WDR57, is a gene found in many organisms, including, but not limited to Homo sapiens, Gallus gallus, Pan troglodytes, Canus familiaris, Bos taurus, Mus musculus, and Rattus norvegicus.

Probable ATP-dependent RNA helicase DDX23 is an enzyme that in humans is encoded by the DDX23 gene.

Thioredoxin-like protein 4A is a protein that is encoded by the TXNL4A gene in humans.

Probable ATP-dependent RNA helicase DDX46 is an enzyme that in humans is encoded by the DDX46 gene.

References

1 2 3 GRCh38: Ensembl release 89: ENSG00000108883 - Ensembl, May 2017
1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000020929 - Ensembl, May 2017
↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ Fabrizio P, Laggerbauer B, Lauber J, Lane WS, Lührmann R (Jul 1997). "An evolutionarily conserved U5 snRNP-specific protein is a GTP-binding factor closely related to the ribosomal translocase EF-2". The EMBO Journal. 16 (13): 4092–106. doi:10.1093/emboj/16.13.4092. PMC 1170032 . PMID 9233818.
↑ "Entrez Gene: EFTUD2 elongation factor Tu GTP binding domain containing 2".
↑ Lines MA, Huang L, Schwartzentruber J, Douglas SL, Lynch DC, Beaulieu C, Guion-Almeida ML, Zechi-Ceide RM, Gener B, Gillessen-Kaesbach G, Nava C, Baujat G, Horn D, Kini U, Caliebe A, Alanay Y, Utine GE, Lev D, Kohlhase J, Grix AW, Lohmann DR, Hehr U, Böhm D, Majewski J, Bulman DE, Wieczorek D, Boycott KM (Feb 2012). "Haploinsufficiency of a spliceosomal GTPase encoded by EFTUD2 causes mandibulofacial dysostosis with microcephaly". American Journal of Human Genetics. 90 (2): 369–77. doi:10.1016/j.ajhg.2011.12.023. PMC 3276671 . PMID 22305528.
↑ Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948 . PMID 17353931.
1 2 Achsel T, Ahrens K, Brahms H, Teigelkamp S, Lührmann R (Nov 1998). "The human U5-220kD protein (hPrp8) forms a stable RNA-free complex with several U5-specific proteins, including an RNA unwindase, a homologue of ribosomal elongation factor EF-2, and a novel WD-40 protein". Molecular and Cellular Biology. 18 (11): 6756–66. doi:10.1128/mcb.18.11.6756. PMC 109259 . PMID 9774689.

Nomura N, Miyajima N, Sazuka T, Tanaka A, Kawarabayasi Y, Sato S, Nagase T, Seki N, Ishikawa K, Tabata S (1995). "Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1". DNA Research. 1 (1): 27–35. CiteSeerX 10.1.1.494.264 . doi: 10.1093/dnares/1.1.27 . PMID 7584026.
Nomura N, Miyajima N, Sazuka T, Tanaka A, Kawarabayasi Y, Sato S, Nagase T, Seki N, Ishikawa K, Tabata S (1995). "Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1 (supplement)". DNA Research. 1 (1): 47–56. doi: 10.1093/dnares/1.1.47 . PMID 7584028.
Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Achsel T, Ahrens K, Brahms H, Teigelkamp S, Lührmann R (Nov 1998). "The human U5-220kD protein (hPrp8) forms a stable RNA-free complex with several U5-specific proteins, including an RNA unwindase, a homologue of ribosomal elongation factor EF-2, and a novel WD-40 protein". Molecular and Cellular Biology. 18 (11): 6756–66. doi:10.1128/mcb.18.11.6756. PMC 109259 . PMID 9774689.
Makarov EM, Makarova OV, Achsel T, Lührmann R (May 2000). "The human homologue of the yeast splicing factor prp6p contains multiple TPR elements and is stably associated with the U5 snRNP via protein-protein interactions". Journal of Molecular Biology. 298 (4): 567–75. doi:10.1006/jmbi.2000.3685. PMID 10788320.
Jurica MS, Licklider LJ, Gygi SR, Grigorieff N, Moore MJ (Apr 2002). "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis". RNA. 8 (4): 426–39. doi:10.1017/S1355838202021088. PMC 1370266 . PMID 11991638.
Peng R, Dye BT, Pérez I, Barnard DC, Thompson AB, Patton JG (Oct 2002). "PSF and p54nrb bind a conserved stem in U5 snRNA". RNA. 8 (10): 1334–47. doi:10.1017/S1355838202022070. PMC 1370341 . PMID 12403470.
Ajuh P, Chusainow J, Ryder U, Lamond AI (Dec 2002). "A novel function for human factor C1 (HCF-1), a host protein required for herpes simplex virus infection, in pre-mRNA splicing". The EMBO Journal. 21 (23): 6590–602. doi:10.1093/emboj/cdf652. PMC 136956 . PMID 12456665.
Li J, Hawkins IC, Harvey CD, Jennings JL, Link AJ, Patton JG (Nov 2003). "Regulation of alternative splicing by SRrp86 and its interacting proteins". Molecular and Cellular Biology. 23 (21): 7437–47. doi:10.1128/MCB.23.21.7437-7447.2003. PMC 207616 . PMID 14559993.
Will CL, Schneider C, Hossbach M, Urlaub H, Rauhut R, Elbashir S, Tuschl T, Lührmann R (Jun 2004). "The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome". RNA. 10 (6): 929–41. doi:10.1261/rna.7320604. PMC 1370585 . PMID 15146077.
Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (Aug 2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the United States of America. 101 (33): 12130–5. Bibcode:2004PNAS..10112130B. doi: 10.1073/pnas.0404720101 . PMC 514446 . PMID 15302935.
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Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948 . PMID 17353931.

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[refGRCh38Ensembl-1] 1 2 3 GRCh38: Ensembl release 89: ENSG00000108883 - Ensembl, May 2017

[refGRCm38Ensembl-2] 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000020929 - Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid9233818-5] Fabrizio P, Laggerbauer B, Lauber J, Lane WS, Lührmann R (Jul 1997). "An evolutionarily conserved U5 snRNP-specific protein is a GTP-binding factor closely related to the ribosomal translocase EF-2". The EMBO Journal. 16 (13): 4092–106. doi:10.1093/emboj/16.13.4092. PMC 1170032 . PMID 9233818.

[entrez-6] "Entrez Gene: EFTUD2 elongation factor Tu GTP binding domain containing 2".

[7] Lines MA, Huang L, Schwartzentruber J, Douglas SL, Lynch DC, Beaulieu C, Guion-Almeida ML, Zechi-Ceide RM, Gener B, Gillessen-Kaesbach G, Nava C, Baujat G, Horn D, Kini U, Caliebe A, Alanay Y, Utine GE, Lev D, Kohlhase J, Grix AW, Lohmann DR, Hehr U, Böhm D, Majewski J, Bulman DE, Wieczorek D, Boycott KM (Feb 2012). "Haploinsufficiency of a spliceosomal GTPase encoded by EFTUD2 causes mandibulofacial dysostosis with microcephaly". American Journal of Human Genetics. 90 (2): 369–77. doi:10.1016/j.ajhg.2011.12.023. PMC 3276671 . PMID 22305528.

[pmid17353931-8] Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948 . PMID 17353931.

[pmid9774689-9] 1 2 Achsel T, Ahrens K, Brahms H, Teigelkamp S, Lührmann R (Nov 1998). "The human U5-220kD protein (hPrp8) forms a stable RNA-free complex with several U5-specific proteins, including an RNA unwindase, a homologue of ribosomal elongation factor EF-2, and a novel WD-40 protein". Molecular and Cellular Biology. 18 (11): 6756–66. doi:10.1128/mcb.18.11.6756. PMC 109259 . PMID 9774689.

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EFTUD2

Contents

Disease associations

Interactions

Related Research Articles

References

Further reading