GTF3C5

GTF3C5
Identifiers
Aliases	GTF3C5 , TFIIIC63, TFIIICepsilon, TFiiiC2-63, general transcription factor IIIC subunit 5
External IDs	OMIM: 604890 MGI: 1917489 HomoloGene: 40806 GeneCards: GTF3C5
Gene location (Human) Chr. Chromosome 9 (human) [1] Band 9q34.13 Start 133,030,675 bp [1] End 133,058,503 bp [1]
Gene location (Mouse) Chr. Chromosome 2 (mouse) [2] Band 2|2 A3 Start 28,456,323 bp [2] End 28,473,763 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in right uterine tube anterior pituitary canal of the cervix tibial nerve skin of abdomen left uterine tube right lobe of thyroid gland left lobe of thyroid gland right adrenal gland ganglionic eminence Top expressed in yolk sac neural tube internal carotid artery external carotid artery spermatocyte morula ganglionic eminence lip dermis mirror More reference expression data BioGPS More reference expression data
Gene ontology Molecular function DNA binding protein binding RNA polymerase III type 1 promoter sequence-specific DNA binding RNA polymerase III type 2 promoter sequence-specific DNA binding RNA polymerase III general transcription initiation factor activity Cellular component transcription factor TFIIIC complex nucleus nucleoplasm Biological process skeletal muscle cell differentiation tRNA transcription by RNA polymerase III 5S class rRNA transcription by RNA polymerase III transcription, DNA-templated transcription by RNA polymerase III RNA polymerase III preinitiation complex assembly transcription initiation from RNA polymerase III promoter Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 9328 70239 Ensembl ENSG00000148308 ENSMUSG00000026816 UniProt Q9Y5Q8 Q8R2T8 RefSeq (mRNA) NM_001122823 NM_001286709 NM_012087 NM_001290484 NM_148928 RefSeq (protein) NP_001116295 NP_001273638 NP_036219 NP_001277413 NP_683730 Location (UCSC) Chr 9: 133.03 – 133.06 Mb Chr 2: 28.46 – 28.47 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

General transcription factor 3C polypeptide 5 is a protein that in humans is encoded by the GTF3C5 gene. ^{[5] [6]}

Model organisms

Gtf3c5 knockout mouse phenotype

Characteristic	Phenotype
Homozygote viability	Abnormal
Recessive lethal study	Abnormal
Fertility	Normal
Body weight	Normal
Anxiety	Normal
Neurological assessment	Normal
Grip strength	Normal
Hot plate	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Body temperature	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology	Normal
Peripheral blood lymphocytes	Normal
Micronucleus test	Normal
Heart weight	Normal
Eye Histopathology	Normal
Salmonella infection	Normal [7]
Citrobacter infection	Normal [8]
All tests and analysis from [9] [10]

Model organisms have been used in the study of GTF3C5 function. A conditional knockout mouse line, called Gtf3c5^{tm2a(KOMP)Wtsi [11] [12]} was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists. ^{[13] [14] [15]}

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion. ^{[9] [16]} Twenty four tests were carried out on mutant mice and two significant abnormalities were observed. ^[9] No homozygous mutant embryos were identified during gestation, and therefore none survived until weaning. The remaining tests were carried out on heterozygous mutant adult mice; no additional significant abnormalities were observed in these animals. ^[9]

Interactions

GTF3C5 has been shown to interact with GTF3C2 ^[5] and GTF3C4. ^[17]

References

1. GRCh38: Ensembl release 89: ENSG00000148308 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000026816 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Hsieh YJ, Wang Z, Kovelman R, Roeder RG (Jul 1999). "Cloning and characterization of two evolutionarily conserved subunits (TFIIIC102 and TFIIIC63) of human TFIIIC and their involvement in functional interactions with TFIIIB and RNA polymerase III". Mol. Cell. Biol. 19 (7): 4944–52. doi:10.1128/mcb.19.7.4944. PMC   84305 . PMID   10373544.
6. "Entrez Gene: GTF3C5 general transcription factor IIIC, polypeptide 5, 63kDa".
7. "Salmonella infection data for Gtf3c5". Wellcome Trust Sanger Institute.
8. "Citrobacter infection data for Gtf3c5". Wellcome Trust Sanger Institute.
9. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. S2CID   85911512.
10. Mouse Resources Portal, Wellcome Trust Sanger Institute.
11. "International Knockout Mouse Consortium".
12. "Mouse Genome Informatics".
13. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC   3572410 . PMID   21677750.
14. Dolgin E (2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi: 10.1038/474262a . PMID   21677718.
15. Collins FS, Rossant J, Wurst W (2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi: 10.1016/j.cell.2006.12.018 . PMID   17218247. S2CID   18872015.
16. van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biol. 12 (6): 224. doi: 10.1186/gb-2011-12-6-224 . PMC   3218837 . PMID   21722353.
17. Hsieh YJ, Kundu TK, Wang Z, Kovelman R, Roeder RG (Nov 1999). "The TFIIIC90 subunit of TFIIIC interacts with multiple components of the RNA polymerase III machinery and contains a histone-specific acetyltransferase activity". Mol. Cell. Biol. 19 (11): 7697–704. doi:10.1128/mcb.19.11.7697. PMC   84812 . PMID   10523658.

Further reading

• Jang KL, Collins MK, Latchman DS (1992). "The human immunodeficiency virus tat protein increases the transcription of human Alu repeated sequences by increasing the activity of the cellular transcription factor TFIIIC". J. Acquir. Immune Defic. Syndr. 5 (11): 1142–7. PMID   1403646.
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID   8125298.
• Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA (1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID   8619474.
• Yu W, Andersson B, Worley KC, Muzny DM, Ding Y, Liu W, Ricafrente JY, Wentland MA, Lennon G, Gibbs RA (1997). "Large-scale concatenation cDNA sequencing". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC   139146 . PMID   9110174.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID   9373149.
• Hsieh YJ, Kundu TK, Wang Z, Kovelman R, Roeder RG (1999). "The TFIIIC90 subunit of TFIIIC interacts with multiple components of the RNA polymerase III machinery and contains a histone-specific acetyltransferase activity". Mol. Cell. Biol. 19 (11): 7697–704. doi:10.1128/mcb.19.11.7697. PMC   84812 . PMID   10523658.
• Dumay-Odelot H, Marck C, Durrieu-Gaillard S, Lefebvre O, Jourdain S, Prochazkova M, Pflieger A, Teichmann M (2007). "Identification, molecular cloning, and characterization of the sixth subunit of human transcription factor TFIIIC". J. Biol. Chem. 282 (23): 17179–89. doi: 10.1074/jbc.M611542200 . PMID   17409385.