IPO5

IPO5
Identifiers
Aliases	IPO5 , IMB3, KPNB3, Pse1, RANBP5, imp5, importin 5
External IDs	OMIM: 602008; MGI: 1917822; HomoloGene: 1710; GeneCards: IPO5; OMA:IPO5 - orthologs
Gene location (Human) Chr. Chromosome 13 (human) [1] Band 13q32.2 Start 97,953,658 bp [1] End 98,024,296 bp [1]
Gene location (Mouse) Chr. Chromosome 14 (mouse) [2] Band 14|14 E4- E5 Start 121,148,636 bp [2] End 121,185,411 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in sperm left testis right testis ventricular zone embryo cartilage tissue ganglionic eminence body of pancreas gastrocnemius muscle stromal cell of endometrium Top expressed in seminiferous tubule primitive streak epiblast somite lens tail of embryo spermatid medullary collecting duct ventricular zone mandibular prominence More reference expression data BioGPS More reference expression data
Gene ontology Molecular function GTPase inhibitor activity protein binding nuclear localization sequence binding RNA binding nuclear import signal receptor activity Cellular component membrane nuclear periphery nuclear pore nucleolus nucleus nuclear membrane cytoplasm Biological process ribosomal protein import into nucleus cellular response to amino acid stimulus protein transport positive regulation of protein import into nucleus viral process intracellular protein transport negative regulation of GTPase activity NLS-bearing protein import into nucleus transport protein import into nucleus negative regulation of cyclin-dependent protein serine/threonine kinase activity Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 3843 70572 Ensembl ENSG00000065150 ENSMUSG00000030662 UniProt O00410 Q8BKC5 RefSeq (mRNA) NM_002271 NM_023579 NM_001360602 RefSeq (protein) NP_002262 NP_076068 NP_001347531 Location (UCSC) Chr 13: 97.95 – 98.02 Mb Chr 14: 121.15 – 121.19 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Importin-5 is a protein that in humans is encoded by the IPO5 gene. ^{[5] [6] [7]} The protein encoded by this gene is a member of the importin beta family. Structurally, the protein adopts the shape of a right hand solenoid and is composed of 24 HEAT repeats. ^[8]

Function

Nuclear transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. ^[9]

IPO5 facilitates cytoplasmic polyadenylation element-binding protein (CPEB)3 translocation by binding to RRM1 motif of CPEB3 in neurons. NMDAR signaling increases RanBP1 expression and reduces the level of cytoplasmic GTP-bound Ran. These changes enhance CPEB3–IPO5 interaction, which consequently accelerates the nuclear import of CPEB3 and promotes its nuclear function. ^[10]

References

1. GRCh38: Ensembl release 89: ENSG00000065150 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000030662 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Yaseen NR, Blobel G (April 1997). "Cloning and characterization of human karyopherin beta3". Proceedings of the National Academy of Sciences of the United States of America. 94 (9): 4451–6. Bibcode:1997PNAS...94.4451Y. doi: 10.1073/pnas.94.9.4451 . PMC   20743 . PMID   9114010.
6. Deane R, Schäfer W, Zimmermann HP, Mueller L, Görlich D, Prehn S, et al. (September 1997). "Ran-binding protein 5 (RanBP5) is related to the nuclear transport factor importin-beta but interacts differently with RanBP1". Molecular and Cellular Biology. 17 (9): 5087–96. doi:10.1128/mcb.17.9.5087. PMC   232359 . PMID   9271386.
7. Deng T, Engelhardt OG, Thomas B, Akoulitchev AV, Brownlee GG, Fodor E (December 2006). "Role of ran binding protein 5 in nuclear import and assembly of the influenza virus RNA polymerase complex". Journal of Virology. 80 (24): 11911–9. doi:10.1128/JVI.01565-06. PMC   1676300 . PMID   17005651.
8. Swale C, Da Costa B, Sedano L, Garzoni F, McCarthy AA, Berger I, et al. (May 2020). "X-ray Structure of the Human Karyopherin RanBP5, an Essential Factor for Influenza Polymerase Nuclear Trafficking" (PDF). Journal of Molecular Biology. 432 (10): 3353–3359. doi:10.1016/j.jmb.2020.03.021. PMID   32222384. S2CID   219670671.
9. "Entrez Gene: RANBP5 RAN binding protein 5".
10. Chao HW, Lai YT, Lu YL, Lin CL, Mai W, Huang YS (September 2012). "NMDAR signaling facilitates the IPO5-mediated nuclear import of CPEB3". Nucleic Acids Research. 40 (17): 8484–98. doi:10.1093/nar/gks598. PMC   3458550 . PMID   22730302.

Further reading

• Bukrinsky MI, Haffar OK (December 1997). "HIV-1 nuclear import: in search of a leader". Frontiers in Bioscience. 2 (4): d578-87. doi: 10.2741/A213 . PMID   9366553.
• Bukrinsky MI, Haffar OK (March 1998). "HIV-1 nuclear import: matrix protein is back on center stage, this time together with Vpr". Molecular Medicine. 4 (3): 138–43. doi:10.1007/BF03401911. PMC   2230352 . PMID   9562972.
• Christophe D, Christophe-Hobertus C, Pichon B (May 2000). "Nuclear targeting of proteins: how many different signals?". Cellular Signalling. 12 (5): 337–41. doi:10.1016/S0898-6568(00)00077-2. PMID   10822175.
• Chook YM, Blobel G (December 2001). "Karyopherins and nuclear import". Current Opinion in Structural Biology. 11 (6): 703–15. doi:10.1016/S0959-440X(01)00264-0. PMID   11751052.
• Bukrinsky MI, Sharova N, Dempsey MP, Stanwick TL, Bukrinskaya AG, Haggerty S, Stevenson M (July 1992). "Active nuclear import of human immunodeficiency virus type 1 preintegration complexes". Proceedings of the National Academy of Sciences of the United States of America. 89 (14): 6580–4. Bibcode:1992PNAS...89.6580B. doi: 10.1073/pnas.89.14.6580 . PMC   49545 . PMID   1631159.
• Bukrinsky MI, Haggerty S, Dempsey MP, Sharova N, Adzhubel A, Spitz L, et al. (October 1993). "A nuclear localization signal within HIV-1 matrix protein that governs infection of non-dividing cells". Nature. 365 (6447): 666–9. Bibcode:1993Natur.365..666B. doi:10.1038/365666a0. PMC   9524217 . PMID   8105392. S2CID   25678.
• Maruyama K, Sugano S (January 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID   8125298.
• Bonaldo MF, Lennon G, Soares MB (September 1996). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Research. 6 (9): 791–806. doi: 10.1101/gr.6.9.791 . PMID   8889548.
• Gallay P, Hope T, Chin D, Trono D (September 1997). "HIV-1 infection of nondividing cells through the recognition of integrase by the importin/karyopherin pathway". Proceedings of the National Academy of Sciences of the United States of America. 94 (18): 9825–30. Bibcode:1997PNAS...94.9825G. doi: 10.1073/pnas.94.18.9825 . PMC   23276 . PMID   9275210.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (October 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID   9373149.
• Henderson BR, Percipalle P (December 1997). "Interactions between HIV Rev and nuclear import and export factors: the Rev nuclear localisation signal mediates specific binding to human importin-beta". Journal of Molecular Biology. 274 (5): 693–707. doi:10.1006/jmbi.1997.1420. PMID   9405152.
• Efthymiadis A, Briggs LJ, Jans DA (January 1998). "The HIV-1 Tat nuclear localization sequence confers novel nuclear import properties". The Journal of Biological Chemistry. 273 (3): 1623–8. doi: 10.1074/jbc.273.3.1623 . PMID   9430704.
• Vodicka MA, Koepp DM, Silver PA, Emerman M (January 1998). "HIV-1 Vpr interacts with the nuclear transport pathway to promote macrophage infection". Genes & Development. 12 (2): 175–85. doi:10.1101/gad.12.2.175. PMC   316441 . PMID   9436978.
• Popov S, Rexach M, Zybarth G, Reiling N, Lee MA, Ratner L, et al. (February 1998). "Viral protein R regulates nuclear import of the HIV-1 pre-integration complex". The EMBO Journal. 17 (4): 909–17. doi:10.1093/emboj/17.4.909. PMC   1170440 . PMID   9463369.
• Popov S, Rexach M, Ratner L, Blobel G, Bukrinsky M (May 1998). "Viral protein R regulates docking of the HIV-1 preintegration complex to the nuclear pore complex". The Journal of Biological Chemistry. 273 (21): 13347–52. doi: 10.1074/jbc.273.21.13347 . PMID   9582382.
• Fouchier RA, Meyer BE, Simon JH, Fischer U, Albright AV, González-Scarano F, Malim MH (July 1998). "Interaction of the human immunodeficiency virus type 1 Vpr protein with the nuclear pore complex". Journal of Virology. 72 (7): 6004–13. doi:10.1128/JVI.72.7.6004-6013.1998. PMC   110405 . PMID   9621063.