After completing his post-doc in 1971,Brown joined the Department of Microbiology in the School of Medicine at the University of Virginia as Assistant Professor and ascended through the ranks to Professor in 1987. During the summers between 1977 and 1980 he also held an appointment at the Marine Biological Laboratory at Woods Hole,Massachusetts,where he taught Physiology. He is currently a professor emeritus of microbiology,immunology,and cancer biology at University of Virginia School of Medicine.[6]
He held appointments as a vice-chair in 1998 and a chair in 1999,under the cancer study section at the American Cancer Society.[1]
Research
Brown's research primarily focuses on the areas of molecular biology and microbiology. At the beginning of his career,he explored several aspects of molecular biology,with a particular focus on the genetic code. Later his work was aimed at understanding the fundamentals of microbiology and virology and several aspects related to it. Thereafter,he and his team began to work on the structural and functional properties of herpes simplex virus. More recently,he has branched out into human gene expression.[7]
Brown's current research revolves around the control of human gene expression and Organization of transcription factor binding sites in the promoters of human genes. He has co-written more than 100 peer-reviewed articles for leading journals and has been cited broadly throughout his career.[8]
Molecular Biology
Brown began his work in molecular biology with a substantial interest in the genetic code. During his PhD,under the supervision of Paul Doty,he proposed the development of new methods to synthesize oligonucleotides of defined sequence and studied their coding properties.[9] Subsequently,he demonstrated that the incorporation of N-formylmethionine is stimulated by oligonucleotide messengers that contain the sequence AUG near or at the 5’end of the messenger RNA chain and that it does not stimulate unformulated methionine.[10] He further revealed that AUG is capable of encoding methionine for both initiating and extension methionines.[11]
Following that Brown has worked on the surface glycoproteins and revealed a cell surface glycoprotein characterized as a differentiated state of neuroblastoma C-1300 cells.[12] In his research,he has elucidated significant differences in the amounts of 4 glycopeptide classes extracted from vertebrate cells and also detected a glycopeptide which was only present during the cell-division period and named it glycol-peptide 4.[13] He also identified differential properties of cell surface membrane from the internal membrane,and particularly highlighted that the isolation of glycoproteins with smooth membrane fraction of cell homogenates characterizes them from internal glycoproteins. Based on these criteria,he isolated two molecular weight classes of glycoproteins as the constituents of the plasma membrane of mouse L-929 cells[14] and suggested that band 1 polypeptide might be efficiently involved in regulating L cell growth.[15]
Whilst working on gene expression,he proposed that for the protection of promoter's ability to regulate gene expression from the mutagenic damage the transcription binding sites of promoters are arrayed in multiple forms and ascertained supportive evidence in brain and liver-specific gene expression.[16]
Microbiology
Brown has worked widely on vesicular stomatitis virus,herpes simplex virus,and human papilloma virus. His research justified the view that the vesicular stomatitis virus matrix M (VSV-M) protein plays a vital role in the maintenance of nucleocapsid in a compact form[17] and mentioned the possibility of identical functionality of VSV-M in-vivo.[18] While working on the physical characteristics of N protein of vesicular stomatitis virus through electron microscopy images he inferred that the N protein has a bilobed structure and is wedge-shaped with an approximate 9.0nm,5.0nm,and 3.3nm of length,depth,and width respectively.[19] In 1991,he started working on Human papilloma virus and published its structure using cryoelectron microscopy. His work clarified that the structural capsid hexon of the human papilloma virus is molecular pentameres.[20]
A major part of Brown's work was on herpes simplex virus (HSV) and mainly focuses on its structure[2] and assembly to determine properties that can aid in the development of novel anti-herpes therapeutic drugs. He illustrated the molecular composition of the capsid pentons and the triplexes of HSV 1 using the guanidine-HCl extraction[21] and discovered that the protein of abortive capsids of equine herpes virus is entirely encapsulated within the capsid.[22] Later,he detected that the HSV capsid assembles in a cell-free environment with the help of four capsid proteins,including VP5 as the major capsid protein,VP22a as a scaffolding protein and VP19c and VP23 as triplex proteins.[23] In related research,he discovered that a mature,icosahedral herpes simplex virus capsid is formed by a spherical intermediate named procapsid,[24] studied the maturation of procapsid through cryoelectron microscopy[25] and described the distinct structural and conformational changes that occur when a procapsid and is transformed into a mature one.[26] Furthermore,he identified the portal that allows entry and exit of herpes virus DNA from the capsid,at the capsid vertex and also demonstrated the isolation of intact capsid from insect cells that produce pUL6 protein.[27]
Bibliography
Books
Volk, Wesley A.; Brown, Jay C. (1997). Basic Microbiology. Benjamin Cummings. ISBN978-0-673-99560-5.
Flickinger, Charles J. (1979). Medical Cell Biology. Saunders. ISBN978-0-7216-3721-1.
Selected articles
Brown, Jay C.; Newcomb, William W.; Lawrenz-Smith, Susan (December 1988). "pH-dependent accumulation of the vesicular stomatitis virus glycoprotein at the ends of intact virions". Virology. 167 (2): 625–629. doi:10.1016/0042-6822(88)90126-2. PMID2849241.
1 2 Newcomb, William W.; Trus, Benes L.; Booy, Frank P.; Steven, Alasdair C.; Wall, Joseph S.; Brown, Jay C. (July 1993). "Structure of the Herpes Simplex Virus Capsid Molecular Composition of the Pentons and the Triplexes". Journal of Molecular Biology. 232 (2): 499–511. doi:10.1006/jmbi.1993.1406. PMID8393939.[non-primary source needed]
↑ Brown, J.C.; Doty, P. (February 1971). "Kinetic preference for initiation of protein synthesis at AUG codons". Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis. 228 (3): 746–748. doi:10.1016/0005-2787(71)90740-4. PMID4929431.[non-primary source needed]
↑ Brown, J. C. (January 1972). "Cell surface glycoproteins I: Accumulation of a glycoprotein on the outer surface of mouse LS cells during mitosis". Journal of Supramolecular Structure. 1 (1): 1–7. doi:10.1002/jss.400010102. PMID4650442.[non-primary source needed]
↑ Hunt, Richard C.; Gold, Elizabeth; Brown, Jay C. (December 1975). "Cell cycle dependent exposure of a high molecular weight protein on the surface of mouse L cells". Biochimica et Biophysica Acta (BBA) - Biomembranes. 413 (3): 453–458. doi:10.1016/0005-2736(75)90128-5. PMID1238124.[non-primary source needed]
↑ Heymann, J. Bernard; Cheng, Naiqian; Newcomb, William W.; Trus, Benes L.; Brown, Jay C.; Steven, Alasdair C. (May 2003). "Dynamics of herpes simplex virus capsid maturation visualized by time-lapse cryo-electron microscopy". Nature Structural & Molecular Biology. 10 (5): 334–341. doi:10.1038/nsb922. PMID12704429.[non-primary source needed]
↑ Trus, Benes L.; Booy, Frank P.; Newcomb, William W.; Brown, Jay C.; Homa, Fred L.; Thomsen, Darrell R.; Steven, Alasdair C. (November 1996). "The herpes simplex virus procapsid: structure, conformational changes upon maturation, and roles of the triplex proteins VP19c and VP23 in assembly". Journal of Molecular Biology. 263 (3): 447–462. doi:10.1016/S0022-2836(96)80018-0. PMID8918600.[non-primary source needed]
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