Lipoarabinomannan

Last updated

Lipoarabinomannan, also called LAM, is a glycolipid, and a virulence factor associated with Mycobacterium tuberculosis , the bacteria responsible for tuberculosis. Its primary function is to inactivate macrophages and scavenge oxidative radicals.

Contents

The inactivation of macrophages allows for the dissemination of mycobacteria to other parts of the body. The destruction of oxidative radicals allows for the survival of the bacteria, as oxidative free radicals are an important mechanism by which our bodies try to rid ourselves of infection.

Background

Lipoarabinomannan is a lipoglycan and major virulence factor in the bacteria genus Mycobacterium. In addition to serving as a major cell wall component, it is thought to serve as a modulin with immunoregulatory and anti-inflammatory effects. This allows the bacterium to maintain survival in the human reservoir by undermining host resistance and acquired immune responses. [1] These mechanisms include the inhibition of T-cell proliferation and of macrophage microbicidal activity via diminished IFN-γ response. [2] Additional functions of Lipoarabinomannan are thought to include the neutralization of cytotoxic oxygen free radicals produced by macrophages, inhibition of protein kinase C, and induction of early response genes. [3]

Structure

Lipoarabinomannan is synthesized via addition of mannose residues to phosphoinositol by a series of mannosyltransferases to produce PIMs and lipomannan (LM). [4] [5] [6] PIM and LM are then glycosylated with arabinan to form LAM. [7] LAM is known to have three primary structural domains. These include a glycosylphosphatidyl anchor which attaches the molecule to the cell wall, a D-mannan core serving as a carbohydrate skeleton, and a terminal D-arabinan, also composing the carbohydrate skeleton. [7] Many arabinofuranosyl side chains branch off the mannose core. [8] It is the covalent modifications to this terminal D-arabinan that creates various LAM structures with their own unique functions to mediate bacterial survival within a host. The presence and the structure of capping allow classification of LAM molecules into three major classes.

ManLAM

Mannosylated LAMs (ManLAM) are characterized by the presence of mannosyl caps on the terminal D-arabinan. These types of LAMs are most commonly found in more pathogenic Mycobacterium species such as M. tuberculosis, M. leprae, and M. bovis. ManLAM has been shown to be an anti-inflammatory molecule that inhibits production of TNF-α and IL-12 production by human dendritic cells and human macrophages in vitro and to modulate M. tuberculosis-induced macrophage apoptosis via binding to host macrophage mannose receptors. [1] [9] This is particularly important in deactivating host macrophages to allow the bacteria to survive and multiply within them. [2]

Proposed Mechanisms

There are many proposed mechanisms behind ManLAM function. Activation of a PI3K pathway is sufficient to trigger phosphorylation of the Bcl-2 family member Bad by ManLAM. ManLAM is able to activate the serine/threonine kinase Akt via phosphorylation which is then able to phosphorylate Bad. Dephosphorylated Bad serves as a pro-apoptotic protein and its activation allows for cell survival. This demonstrates one virulence-associated mechanism by which bacteria are able to up-regulate signaling pathways to control host cell apoptosis. [8]

ManLAM may also directly activate SHP-1, a phosphotyrosine phosphatase known to be involved in terminating activation signals. SHP-1 negatively regulates pathways related to the actions of IFN-γ and insulin. LAM may regulate SHP-1 by multiple mechanisms including direct interactions, phosphorylation, and subcellular localization. Once activated, SHP-1 translocates from the cytosol to the membrane. By activating a phosphatase, LAM can inhibit LPS and IFN-γ induced protein tyrosine phosphorylation in monocytes. This decreases production of TNF-α, a molecule necessary in forming granulomas against M. tuberculosis and important in macrophage defense against bacterium via nitrogen oxide production. LAM's activation of SHP-1 also works to deactivate IL-12. IL-12 is important for innate resistance to M. tuberculosis infections. It activates natural killer cells which produce IFN-γ to activate macrophages. By impairing the function of these two molecules by SHP-1 activation, ManLAM may promote intracellular survival. [2]

Other models suggest that ManLAM acts to mediate immunosuppressive effects through suppression of LPS-induced IL-12 p40 protein production. ManLAM is thought to inhibit the IL-1 receptor-associated kinase (IRAK)-TRAF6 interaction, IκB-α phosphorylation, and nuclear translocation of c-Rel and p50 which causes reduced IL-12 p40 production. [10]

Proposed mechanisms of ManLAM functions ManLAMpathway.jpg
Proposed mechanisms of ManLAM functions

PILAM

LAMS capped with phosphoinositol are typically found in nonpathogenic species including M. smegmatis. In contrast to ManLAMs, PILAMs are pro-inflammatory. CD14, a recognition receptor present on macrophages, associate with toll-like receptor 2 (TLR2) is described to be a receptor for PILAM. [11] Binding of PILAM to the receptor elicits the activation of an intracellular signaling cascade which activates transcription factors that initiate transcription of proinflammatory cytokine genes. This may lead to TNF-α, IL-8, and IL-12 activation and apoptosis of macrophages. [1] [12]

AraLAM (CheLAM)

Certain species of rapid-growing bacterium such as M. chelonae and laboratory strains (H37Ra) contain LAMs that are absent of both mannose and phosphoinosital caps. [1] This form of LAM is characterized by 1,3 –mannosyl side chains instead of the 1,2 commonly found in others mycobacterial species. [12] These forms are considered to be more potent than the mannose-capped ManLAM in inducing functions associated with macrophage activation. [9] In addition to stimulation of early genes such as c-fos, KC, and JE, AraLAM induces transcription of the mRNA for cytokines (such as TNF-α, IL 1-α, IL 1-β, IL-6, IL-8, and IL-10) characteristically produced by macrophages. [2] [9] Proto-oncogenes c-fos and c-myc are involved in the regulation of gene transcription while JE and KC are peptide cytokines that serve as specific chemoattractants for neutrophils and monocytes. [13] Activation of TNF-α creates pathologic manifestations of disease such as tissue necrosis, nerve damage, and protective immunity. [14] O-acyl groups of the arabinomannan moiety may be responsible for TNF-inducing activity which causes the tuberculosis symptoms of fever, weight loss, and necrosis. [15] However, the presence of ManLAMs decreases AraLAM activity, suppressing an immune response. [9]

Point-of-care TB Diagnosis

Fujifilm SILVAMP TB LAM is a LAM based urine point-of-care test, using silver halide amplification technology. [16] Up to 60% of people with HIV are unable to produce a sputum sample, leading to delays in TB diagnosis for these patients, which often proves deadly. [17] Foundation for Innovative New Diagnostics (FIND) and Fujifilm developed the test, which is particularly useful in low-income settings, where the burden of HIV and TB is the highest. It takes an hour, doesn’t rely on electricity, and requires limited training for health workers. [18] A study with 968 HIV+ hospital inpatients found the Fujifilm SILVAMP TB LAM test to have a 28.1% higher sensitivity than the Alere Determine TB LAM Ag and the Fujifilm SILVAMP TB LAM could diagnose 65% of patients with active TB within 24 h. [19] A meta-analysis with 1,595 inpatients and outpatients showed 70.7% sensitivity and 90.9% specificity for TB diagnosis in people living with HIV for Fujifilm SILVAMP TB LAM. Further, FujiLAM showed good sensitivity for the detection of extrapulmonary TB (EPTB) ranging from 47 to 94% across different forms of ETB [20] and could have rapidly diagnosed TB in up to 89% of HIV-positive inpatients who died within 12 weeks. [21] The test showed a high positive predictive value (95.2%) in HIV-negative outpatients and has the potential to improve rapid, urine-based TB diagnosis in general populations at the point-of-care. [22] Large prospective studies are on the way. [23]

Related Research Articles

<span class="mw-page-title-main">Phagosome</span>

In cell biology, a phagosome is a vesicle formed around a particle engulfed by a phagocyte via phagocytosis. Professional phagocytes include macrophages, neutrophils, and dendritic cells (DCs).

Collectins (collagen-containing C-type lectins) are a part of the innate immune system. They form a family of collagenous Ca2+-dependent defense lectins, which are found in animals. Collectins are soluble pattern recognition receptors (PRRs). Their function is to bind to oligosaccharide structure or lipids that are on the surface of microorganisms. Like other PRRs they bind pathogen-associated molecular patterns (PAMPs) and danger-associated molecular patterns (DAMPs) of oligosaccharide origin. Binding of collectins to microorganisms may trigger elimination of microorganisms by aggregation, complement activation, opsonization, activation of phagocytosis, or inhibition of microbial growth. Other functions of collectins are modulation of inflammatory, allergic responses, adaptive immune system and clearance of apoptotic cells.

<span class="mw-page-title-main">Langerin</span>

Langerin (CD207) is a type II transmembrane protein which is encoded by the CD207 gene in humans. It was discovered by scientists Sem Saeland and Jenny Valladeau as a main part of Birbeck granules. Langerin is C-type lectin receptor on Langerhans cells (LCs) and in mice also on dermal interstitial CD103+ dendritic cells (DC) and on resident CD8+ DC in lymph nodes.

The mannose receptor is a C-type lectin primarily present on the surface of macrophages, immature dendritic cells and liver sinusoidal endothelial cells, but is also expressed on the surface of skin cells such as human dermal fibroblasts and keratinocytes. It is the first member of a family of endocytic receptors that includes Endo180 (CD280), M-type PLA2R, and DEC-205 (CD205).

<span class="mw-page-title-main">C5a receptor</span> Mammalian protein found in Homo sapiens

The C5a receptor also known as complement component 5a receptor 1 (C5AR1) or CD88 is a G protein-coupled receptor for C5a. It functions as a complement receptor. C5a receptor 1 modulates inflammatory responses, obesity, development and cancers. From a signaling transduction perspective, C5a receptor 1 activation is implicated in β-arrestin2 recruitment via Rab5a, coupling of Gαi proteins, ERK1/2 phosphorylation, calcium mobilization and Rho activation leading to downstream functions, such as secretion of cytokines, chemotaxis, and phagocytosis.

ESAT-6 or Early Secreted Antigenic Target 6 kDa, is produced by Mycobacterium tuberculosis, it is a secretory protein and potent T cell antigen. It is used in tuberculosis diagnosis by the whole blood interferon γ test QuantiFERON-TB Gold, in conjunction with CFP-10.

<span class="mw-page-title-main">Tyrosine kinase 2</span> Enzyme and coding gene in humans

Non-receptor tyrosine-protein kinase TYK2 is an enzyme that in humans is encoded by the TYK2 gene.

<span class="mw-page-title-main">Janus kinase 2</span> Non-receptor tyrosine kinase and coding gene in humans

Janus kinase 2 is a non-receptor tyrosine kinase. It is a member of the Janus kinase family and has been implicated in signaling by members of the type II cytokine receptor family, the GM-CSF receptor family, the gp130 receptor family, and the single chain receptors.

<span class="mw-page-title-main">CRKL</span> Protein-coding gene in the species Homo sapiens

Crk-like protein is a protein that in humans is encoded by the CRKL gene.

<span class="mw-page-title-main">PIK3R2</span> Protein-coding gene in the species Homo sapiens

Phosphatidylinositol 3-kinase regulatory subunit beta is an enzyme that in humans is encoded by the PIK3R2 gene.

<span class="mw-page-title-main">TRAF5</span> Protein-coding gene in the species Homo sapiens

TNF receptor-associated factor 5 is a protein that in humans is encoded by the TRAF5 gene.

<span class="mw-page-title-main">IRAK1</span> Protein-coding gene in the species Homo sapiens

Interleukin-1 receptor-associated kinase 1 (IRAK-1) is an enzyme in humans encoded by the IRAK1 gene. IRAK-1 plays an important role in the regulation of the expression of inflammatory genes by immune cells, such as monocytes and macrophages, which in turn help the immune system in eliminating bacteria, viruses, and other pathogens. IRAK-1 is part of the IRAK family consisting of IRAK-1, IRAK-2, IRAK-3, and IRAK-4, and is activated by inflammatory molecules released by signaling pathways during pathogenic attack. IRAK-1 is classified as a kinase enzyme, which regulates pathways in both innate and adaptive immune systems.

<span class="mw-page-title-main">Testicular receptor 4</span> Protein-coding gene in the species Homo sapiens

Testicular receptor 4 also known as NR2C2 is a protein that in humans is encoded by the NR2C2 gene.

<span class="mw-page-title-main">LIGHT (protein)</span> Secreted protein of the TNF superfamily

LIGHT, also known as tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. It is recognized by herpesvirus entry mediator (HVEM), as well as decoy receptor 3.

<span class="mw-page-title-main">MAP3K8</span> Protein-coding gene in the species Homo sapiens

Mitogen-activated protein kinase kinase kinase 8 is an enzyme that in humans is encoded by the MAP3K8 gene.

<span class="mw-page-title-main">TSG-6</span> Protein-coding gene in the species Homo sapiens

Tumor necrosis factor-inducible gene 6 protein also known as TNF-stimulated gene 6 protein or TSG-6 is a protein that in humans is encoded by the TNFAIP6 gene.

<span class="mw-page-title-main">Tumor necrosis factor receptor 2</span> Membrane receptor protein found in humans

Tumor necrosis factor receptor 2 (TNFR2), also known as tumor necrosis factor receptor superfamily member 1B (TNFRSF1B) and CD120b, is one of two membrane receptors that binds tumor necrosis factor-alpha (TNFα). Like its counterpart, tumor necrosis factor receptor 1 (TNFR1), the extracellular region of TNFR2 consists of four cysteine-rich domains which allow for binding to TNFα. TNFR1 and TNFR2 possess different functions when bound to TNFα due to differences in their intracellular structures, such as TNFR2 lacking a death domain (DD).

<span class="mw-page-title-main">Cord factor</span> Chemical compound

Cord factor, or trehalose dimycolate (TDM), is a glycolipid molecule found in the cell wall of Mycobacterium tuberculosis and similar species. It is the primary lipid found on the exterior of M. tuberculosis cells. Cord factor influences the arrangement of M. tuberculosis cells into long and slender formations, giving its name. Cord factor is virulent towards mammalian cells and critical for survival of M. tuberculosis in hosts, but not outside of hosts. Cord factor has been observed to influence immune responses, induce the formation of granulomas, and inhibit tumor growth. The antimycobacterial drug SQ109 is thought to inhibit TDM production levels and in this way disrupts its cell wall assembly.

Macrophage polarization is a process by which macrophages adopt different functional programs in response to the signals from their microenvironment. This ability is connected to their multiple roles in the organism: they are powerful effector cells of the innate immune system, but also important in removal of cellular debris, embryonic development and tissue repair.

<span class="mw-page-title-main">Mannose receptor C-type 1</span> Protein-coding gene in the species Homo sapiens

Mannose receptor C-type 1 is a protein that in humans is encoded by the MRC1 gene.

References

  1. 1 2 3 4 Guérardel Y, Maes E, Briken V, Chirat F, Leroy Y, Locht C, et al. (September 2003). "Lipomannan and lipoarabinomannan from a clinical isolate of Mycobacterium kansasii: novel structural features and apoptosis-inducing properties". The Journal of Biological Chemistry. 278 (38): 36637–51. doi: 10.1074/jbc.M305427200 . PMID   12829695.
  2. 1 2 3 4 Knutson KL, Hmama Z, Herrera-Velit P, Rochford R, Reiner NE (January 1998). "Lipoarabinomannan of Mycobacterium tuberculosis promotes protein tyrosine dephosphorylation and inhibition of mitogen-activated protein kinase in human mononuclear phagocytes. Role of the Src homology 2 containing tyrosine phosphatase 1". The Journal of Biological Chemistry. 273 (1): 645–52. doi: 10.1074/jbc.273.1.645 . PMID   9417127.
  3. Chan J, Fan XD, Hunter SW, Brennan PJ, Bloom BR (May 1991). "Lipoarabinomannan, a possible virulence factor involved in persistence of Mycobacterium tuberculosis within macrophages". Infection and Immunity. 59 (5): 1755–61. doi:10.1128/IAI.59.5.1755-1761.1991. PMC   257912 . PMID   1850379.
  4. Korduláková J, Gilleron M, Mikusova K, Puzo G, Brennan PJ, Gicquel B, Jackson M (August 2002). "Definition of the first mannosylation step in phosphatidylinositol mannoside synthesis. PimA is essential for growth of mycobacteria". The Journal of Biological Chemistry. 277 (35): 31335–44. doi: 10.1074/jbc.m204060200 . PMID   12068013.
  5. Lea-Smith DJ, Martin KL, Pyke JS, Tull D, McConville MJ, Coppel RL, Crellin PK (March 2008). "Analysis of a new mannosyltransferase required for the synthesis of phosphatidylinositol mannosides and lipoarbinomannan reveals two lipomannan pools in corynebacterineae". The Journal of Biological Chemistry. 283 (11): 6773–82. doi: 10.1074/jbc.m707139200 . PMID   18178556.
  6. Morita YS, Sena CB, Waller RF, Kurokawa K, Sernee MF, Nakatani F, et al. (September 2006). "PimE is a polyprenol-phosphate-mannose-dependent mannosyltransferase that transfers the fifth mannose of phosphatidylinositol mannoside in mycobacteria". The Journal of Biological Chemistry. 281 (35): 25143–55. doi: 10.1074/jbc.m604214200 . PMID   16803893.
  7. 1 2 Guerardel Y, Maes E, Elass E, Leroy Y, Timmerman P, Besra GS, et al. (August 2002). "Structural study of lipomannan and lipoarabinomannan from Mycobacterium chelonae. Presence of unusual components with alpha 1,3-mannopyranose side chains". The Journal of Biological Chemistry. 277 (34): 30635–48. doi: 10.1074/jbc.M204398200 . PMID   12063260.
  8. 1 2 Maiti D, Bhattacharyya A, Basu J (January 2001). "Lipoarabinomannan from Mycobacterium tuberculosis promotes macrophage survival by phosphorylating Bad through a phosphatidylinositol 3-kinase/Akt pathway". The Journal of Biological Chemistry. 276 (1): 329–33. doi: 10.1074/jbc.M002650200 . PMID   11020382.
  9. 1 2 3 4 Gilleron M, Himoudi N, Adam O, Constant P, Venisse A, Rivière M, Puzo G (January 1997). "Mycobacterium smegmatis phosphoinositols-glyceroarabinomannans. Structure and localization of alkali-labile and alkali-stable phosphoinositides". The Journal of Biological Chemistry. 272 (1): 117–24. doi: 10.1074/jbc.272.1.117 . PMID   8995236.
  10. Pathak SK, Basu S, Bhattacharyya A, Pathak S, Kundu M, Basu J (December 2005). "Mycobacterium tuberculosis lipoarabinomannan-mediated IRAK-M induction negatively regulates Toll-like receptor-dependent interleukin-12 p40 production in macrophages". The Journal of Biological Chemistry. 280 (52): 42794–800. doi: 10.1074/jbc.M506471200 . PMID   16263713.
  11. Yu W, Soprana E, Cosentino G, Volta M, Lichenstein HS, Viale G, Vercelli D (October 1998). "Soluble CD14(1-152) confers responsiveness to both lipoarabinomannan and lipopolysaccharide in a novel HL-60 cell bioassay". Journal of Immunology. 161 (8): 4244–51. PMID   9780199.
  12. 1 2 Vignal C, Guérardel Y, Kremer L, Masson M, Legrand D, Mazurier J, Elass E (August 2003). "Lipomannans, but not lipoarabinomannans, purified from Mycobacterium chelonae and Mycobacterium kansasii induce TNF-alpha and IL-8 secretion by a CD14-toll-like receptor 2-dependent mechanism". Journal of Immunology. 171 (4): 2014–23. doi: 10.4049/jimmunol.171.4.2014 . PMID   12902506.
  13. Roach TI, Barton CH, Chatterjee D, Blackwell JM (March 1993). "Macrophage activation: lipoarabinomannan from avirulent and virulent strains of Mycobacterium tuberculosis differentially induces the early genes c-fos, KC, JE, and tumor necrosis factor-alpha". Journal of Immunology. 150 (5): 1886–96. PMID   8436823.
  14. Barnes PF, Chatterjee D, Brennan PJ, Rea TH, Modlin RL (April 1992). "Tumor necrosis factor production in patients with leprosy". Infection and Immunity. 60 (4): 1441–6. doi:10.1128/IAI.60.4.1441-1446.1992. PMC   257016 . PMID   1548069.
  15. Moreno C, Taverne J, Mehlert A, Bate CA, Brealey RJ, Meager A, et al. (May 1989). "Lipoarabinomannan from Mycobacterium tuberculosis induces the production of tumour necrosis factor from human and murine macrophages". Clinical and Experimental Immunology. 76 (2): 240–5. PMC   1541837 . PMID   2503277.
  16. "Fujifilm and FIND* Sign Development Contract to Develop Highly Sensitive Rapid Tuberculosis Diagnosis Kit for Developing Countries". Fujifilm Corporation. Archived from the original on 20 December 2018.
  17. "Historic UN TB declaration 'falls short' say activists". Health24. 27 September 2018.
  18. "Fujifilm SILVAMP TB LAM test procedure". YouTube .
  19. Broger T, Sossen B, du Toit E, Kerkhoff AD, Schutz C, Ivanova Reipold E, et al. (August 2019). "Novel lipoarabinomannan point-of-care tuberculosis test for people with HIV: a diagnostic accuracy study". The Lancet. Infectious Diseases. 19 (8): 852–861. doi:10.1016/s1473-3099(19)30001-5. PMC   6656794 . PMID   31155318.
  20. Kerkhoff AD, Sossen B, Schutz C, Reipold EI, Trollip A, Moreau E, et al. (February 2020). "Diagnostic sensitivity of SILVAMP TB-LAM (FujiLAM) point-of-care urine assay for extra-pulmonary tuberculosis in people living with HIV". The European Respiratory Journal. 55 (2): 1901259. doi:10.1183/13993003.01259-2019. PMC   7002975 . PMID   31699835.
  21. Sossen B, Broger T, Kerkhoff AD, Schutz C, Trollip A, Moreau E, et al. (January 2020). "'SILVAMP TB LAM' rapid urine tuberculosis test predicts mortality in hospitalized HIV patients in South Africa". Clinical Infectious Diseases. 71 (8): 1973–1976. doi: 10.1093/cid/ciaa024 . PMC   8240995 . PMID   31917832.
  22. Broger T, Nicol M, Sigal G, Gotuzzo E, Zimmer AJ, Surtie S, et al. (July 2020). "Diagnostic accuracy of three urine lipoarabinomannan tuberculosis assays in HIV-negative outpatients". The Journal of Clinical Investigation. 130 (11): 5756–5764. doi: 10.1172/JCI140461 . PMC   7598043 . PMID   32692731.
  23. Clinical trial number NCT04089423 for "FujiLAM Prospective Evaluation Trial" at ClinicalTrials.gov

Further reading