MED21

MED21
Identifiers
Aliases	MED21 , SRB7, SURB7, hSrb7, mediator complex subunit 21
External IDs	OMIM: 603800 MGI: 1347064 HomoloGene: 3148 GeneCards: MED21
Gene location (Human) Chr. Chromosome 12 (human) [1] Band 12p11.23 Start 27,022,546 bp [1] End 27,066,343 bp [1]
Gene location (Mouse) Chr. Chromosome 6 (mouse) [2] Band 6 G3|6 77.7 cM Start 146,642,547 bp [2] End 146,650,732 bp [2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • DNA-directed 5'-3' RNA polymerase activity • GO:0001105 transcription coactivator activity • GO:0001104 transcription coregulator activity • GO:0001948 protein binding • GO:1904264, GO:1904822, GO:0090622, GO:0090302 ubiquitin protein ligase activity Cellular component • nucleus • ubiquitin ligase complex • mediator complex Biological process • regulation of transcription by RNA polymerase II • stem cell population maintenance • regulation of transcription, DNA-templated • protein ubiquitination • blastocyst development • transcription, DNA-templated • positive regulation of transcription by RNA polymerase II • RNA biosynthetic process Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	9412	108098
Ensembl	ENSG00000152944	ENSMUSG00000030291
UniProt	Q13503	Q9CQ39
RefSeq (mRNA)	NM_001271811 NM_004264	NM_025315
RefSeq (protein)	NP_001258740 NP_004255	NP_079591
Location (UCSC)	Chr 12: 27.02 – 27.07 Mb	Chr 6: 146.64 – 146.65 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Mediator of RNA polymerase II transcription subunit 21 is an enzyme that in humans is encoded by the MED21 gene. ^{[5] [6]}

Interactions

MED21 has been shown to interact with:

• BRCA1, ^[7]
• CDK8, ^{[8] [9] [10] [11]}
• GTF2F1, ^{[7] [9]}
• GTF2H4, ^[7]
• MED6, ^[12] and
• POLR2A. ^{[7] [8] [9]}

References

1. GRCh38: Ensembl release 89: ENSG00000152944 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000030291 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Chao DM, Gadbois EL, Murray PJ, Anderson SF, Sonu MS, Parvin JD, Young RA (Apr 1996). "A mammalian SRB protein associated with an RNA polymerase II holoenzyme". Nature. 380 (6569): 82–5. Bibcode:1996Natur.380...82C. doi:10.1038/380082a0. PMID   8598913. S2CID   4349547.
6. "Entrez Gene: SURB7 SRB7 suppressor of RNA polymerase B homolog (yeast)".
7. Scully R, Anderson SF, Chao DM, Wei W, Ye L, Young RA, Livingston DM, Parvin JD (May 1997). "BRCA1 is a component of the RNA polymerase II holoenzyme". Proc. Natl. Acad. Sci. U.S.A. 94 (11): 5605–10. Bibcode:1997PNAS...94.5605S. doi: 10.1073/pnas.94.11.5605 . PMC   20825 . PMID   9159119.
8. Cho H, Orphanides G, Sun X, Yang XJ, Ogryzko V, Lees E, Nakatani Y, Reinberg D (Sep 1998). "A human RNA polymerase II complex containing factors that modify chromatin structure". Mol. Cell. Biol. 18 (9): 5355–63. doi:10.1128/MCB.18.9.5355. PMC   109120 . PMID   9710619.
9. Suñé C, Hayashi T, Liu Y, Lane WS, Young RA, Garcia-Blanco MA (Oct 1997). "CA150, a nuclear protein associated with the RNA polymerase II holoenzyme, is involved in Tat-activated human immunodeficiency virus type 1 transcription". Mol. Cell. Biol. 17 (10): 6029–39. doi:10.1128/MCB.17.10.6029. PMC   232452 . PMID   9315662.
10. Ito M, Yuan CX, Malik S, Gu W, Fondell JD, Yamamura S, Fu ZY, Zhang X, Qin J, Roeder RG (Mar 1999). "Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators". Mol. Cell. 3 (3): 361–70. doi: 10.1016/s1097-2765(00)80463-3 . PMID   10198638.
11. Kang YK, Guermah M, Yuan CX, Roeder RG (Mar 2002). "The TRAP/Mediator coactivator complex interacts directly with estrogen receptors alpha and beta through the TRAP220 subunit and directly enhances estrogen receptor function in vitro". Proc. Natl. Acad. Sci. U.S.A. 99 (5): 2642–7. Bibcode:2002PNAS...99.2642K. doi: 10.1073/pnas.261715899 . PMC   122401 . PMID   11867769.
12. Sun X, Zhang Y, Cho H, Rickert P, Lees E, Lane W, Reinberg D (Aug 1998). "NAT, a human complex containing Srb polypeptides that functions as a negative regulator of activated transcription". Mol. Cell. 2 (2): 213–22. doi: 10.1016/s1097-2765(00)80131-8 . PMID   9734358.

Further reading

• Zheng XM, Black D, Chambon P, Egly JM (1990). "Sequencing and expression of complementary DNA for the general transcription factor BTF3". Nature. 344 (6266): 556–9. Bibcode:1990Natur.344..556Z. doi:10.1038/344556a0. PMID   2320128. S2CID   4227320.
• Maldonado E, Shiekhattar R, Sheldon M, Cho H, Drapkin R, Rickert P, Lees E, Anderson CW, Linn S, Reinberg D (1996). "A human RNA polymerase II complex associated with SRB and DNA-repair proteins". Nature. 381 (6577): 86–9. Bibcode:1996Natur.381...86M. doi:10.1038/381086a0. PMID   8609996. S2CID   4327125.
• Cujec TP, Cho H, Maldonado E, Meyer J, Reinberg D, Peterlin BM (1997). "The human immunodeficiency virus transactivator Tat interacts with the RNA polymerase II holoenzyme". Mol. Cell. Biol. 17 (4): 1817–23. doi:10.1128/MCB.17.4.1817. PMC   232028 . PMID   9121429.
• Scully R, Anderson SF, Chao DM, Wei W, Ye L, Young RA, Livingston DM, Parvin JD (1997). "BRCA1 is a component of the RNA polymerase II holoenzyme". Proc. Natl. Acad. Sci. U.S.A. 94 (11): 5605–10. Bibcode:1997PNAS...94.5605S. doi: 10.1073/pnas.94.11.5605 . PMC   20825 . PMID   9159119.
• Pan G, Aso T, Greenblatt J (1997). "Interaction of elongation factors TFIIS and elongin A with a human RNA polymerase II holoenzyme capable of promoter-specific initiation and responsive to transcriptional activators". J. Biol. Chem. 272 (39): 24563–71. doi: 10.1074/jbc.272.39.24563 . PMID   9305922.
• Cho H, Orphanides G, Sun X, Yang XJ, Ogryzko V, Lees E, Nakatani Y, Reinberg D (1998). "A human RNA polymerase II complex containing factors that modify chromatin structure". Mol. Cell. Biol. 18 (9): 5355–63. doi:10.1128/MCB.18.9.5355. PMC   109120 . PMID   9710619.
• Sun X, Zhang Y, Cho H, Rickert P, Lees E, Lane W, Reinberg D (1998). "NAT, a human complex containing Srb polypeptides that functions as a negative regulator of activated transcription". Mol. Cell. 2 (2): 213–22. doi: 10.1016/S1097-2765(00)80131-8 . PMID   9734358.
• Garriga J, Peng J, Parreño M, Price DH, Henderson EE, Graña X (1999). "Upregulation of cyclin T1/CDK9 complexes during T cell activation". Oncogene. 17 (24): 3093–102. doi: 10.1038/sj.onc.1202548 . PMID   9872325. S2CID   58976.
• Gu W, Malik S, Ito M, Yuan CX, Fondell JD, Zhang X, Martinez E, Qin J, Roeder RG (1999). "A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation". Mol. Cell. 3 (1): 97–108. doi: 10.1016/S1097-2765(00)80178-1 . PMID   10024883.
• Ramanathan Y, Rajpara SM, Reza SM, Lees E, Shuman S, Mathews MB, Pe'ery T (2001). "Three RNA polymerase II carboxyl-terminal domain kinases display distinct substrate preferences". J. Biol. Chem. 276 (14): 10913–20. doi: 10.1074/jbc.M010975200 . PMID   11278802.
• Suzuki H, Fukunishi Y, Kagawa I, Saito R, Oda H, Endo T, Kondo S, Bono H, Okazaki Y, Hayashizaki Y (2001). "Protein-protein interaction panel using mouse full-length cDNAs". Genome Res. 11 (10): 1758–65. doi:10.1101/gr.180101. PMC   311163 . PMID   11591653.
• Ge K, Guermah M, Yuan CX, Ito M, Wallberg AE, Spiegelman BM, Roeder RG (2002). "Transcription coactivator TRAP220 is required for PPAR gamma 2-stimulated adipogenesis". Nature. 417 (6888): 563–7. Bibcode:2002Natur.417..563G. doi:10.1038/417563a. PMID   12037571. S2CID   4432077.
• Sato S, Tomomori-Sato C, Banks CA, Parmely TJ, Sorokina I, Brower CS, Conaway RC, Conaway JW (2004). "A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex". J. Biol. Chem. 278 (50): 49671–4. doi: 10.1074/jbc.C300444200 . PMID   14576168.
• Wallberg AE, Yamamura S, Malik S, Spiegelman BM, Roeder RG (2004). "Coordination of p300-mediated chromatin remodeling and TRAP/mediator function through coactivator PGC-1alpha". Mol. Cell. 12 (5): 1137–49. doi: 10.1016/S1097-2765(03)00391-5 . PMID   14636573.
• Tomomori-Sato C, Sato S, Parmely TJ, Banks CA, Sorokina I, Florens L, Zybailov B, Washburn MP, Brower CS, Conaway RC, Conaway JW (2004). "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2". J. Biol. Chem. 279 (7): 5846–51. doi: 10.1074/jbc.M312523200 . PMID   14638676.
• Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC (2004). "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology". Mol. Cell. 14 (5): 685–91. doi: 10.1016/j.molcel.2004.05.006 . PMID   15175163.
• Nevado J, Tenbaum SP, Aranda A (2005). "hSrb7, an essential human Mediator component, acts as a coactivator for the thyroid hormone receptor". Mol. Cell. Endocrinol. 222 (1–2): 41–51. doi:10.1016/j.mce.2004.05.003. PMID   15249124. S2CID   11164060.