MED27

MED27
Identifiers
Aliases	MED27 , CRAP34, CRSP34, CRSP8, TRAP37, MED3, mediator complex subunit 27, NEDSCAC
External IDs	OMIM: 605044 MGI: 1916225 HomoloGene: 3152 GeneCards: MED27
Gene location (Human) Chr. Chromosome 9 (human) [1] Band 9q34.13 Start 131,852,928 bp [1] End 132,079,867 bp [1]
Gene location (Mouse) Chr. Chromosome 2 (mouse) [2] Band 2 B|2 19.84 cM Start 29,236,831 bp [2] End 29,414,805 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in oocyte endothelial cell secondary oocyte ganglionic eminence islet of Langerhans popliteal artery thoracic aorta ascending aorta hair follicle left coronary artery Top expressed in superior surface of tongue gallbladder plantaris muscle extensor digitorum longus muscle entorhinal cortex corneal stroma secondary oocyte interventricular septum morula extraocular muscle More reference expression data BioGPS More reference expression data
Gene ontology Molecular function transcription coactivator activity protein binding ubiquitin protein ligase activity Cellular component mediator complex nucleolus nucleus transcription regulator complex nucleoplasm ubiquitin ligase complex cytosol Biological process regulation of transcription by RNA polymerase II stem cell population maintenance transcription initiation from RNA polymerase II promoter regulation of transcription, DNA-templated protein ubiquitination transcription, DNA-templated positive regulation of nucleic acid-templated transcription Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 9442 68975 Ensembl ENSG00000160563 ENSMUSG00000026799 UniProt Q6P2C8 Q9DB40 RefSeq (mRNA) NM_001253881 NM_001253882 NM_004269 NM_001290489 NM_026896 NM_029615 NM_001290490 RefSeq (protein) NP_001240810 NP_001240811 NP_004260 NP_001277418 NP_001277419 NP_081172 NP_083891 Location (UCSC) Chr 9: 131.85 – 132.08 Mb Chr 2: 29.24 – 29.41 Mb PubMed search [3] [4]
Wikidata
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Med27
Identifiers
Symbol	Med27
Pfam	PF11571
InterPro	IPR021627
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

Mediator of RNA polymerase II transcription subunit 27 is an enzyme that in humans is encoded by the MED27 gene. ^{[5] [6]} It forms part of the Mediator complex.

The ubiquitous expression of Med27 mRNA suggests a universal requirement for Med27 in transcriptional initiation. Loss of Crsp34/Med27 decreases amacrine cell number, but increases the number of rod photoreceptor cells. ^[7]

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. ^[6]

See also

• Mediator complex

References

1. GRCh38: Ensembl release 89: ENSG00000160563 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000026799 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Ryu S, Zhou S, Ladurner AG, Tjian R (Feb 1999). "The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1". Nature. 397 (6718): 446–50. Bibcode:1999Natur.397..446R. doi:10.1038/17141. PMID   9989412. S2CID   4405569.
6. "Entrez Gene: CRSP8 cofactor required for Sp1 transcriptional activation, subunit 8, 34kDa".
7. Dürr K, Holzschuh J, Filippi A, Ettl AK, Ryu S, Shepherd IT, Driever W (October 2006). "Differential roles of transcriptional mediator complex subunits Crsp34/Med27, Crsp150/Med14 and Trap100/Med24 during zebrafish retinal development". Genetics. 174 (2): 693–705. doi:10.1534/genetics.105.055152. PMC   1602071 . PMID   16582438.

This article incorporates text from the public domain Pfam and InterPro: IPR021627

Further reading

• Roth J, Krause M (1977). "[Relation between manifest bronchial obstruction and bronchial hyperreactivity (author's transl)]". Zeitschrift für Erkrankungen der Atmungsorgane. 146 (1): 12–6. PMID   1020353.
• Gress TM, Müller-Pillasch F, Geng M, et al. (1996). "A pancreatic cancer-specific expression profile". Oncogene. 13 (8): 1819–30. PMID   8895530.
• Malik S, Gu W, Wu W, et al. (2000). "The USA-derived transcriptional coactivator PC2 is a submodule of TRAP/SMCC and acts synergistically with other PCs". Mol. Cell. 5 (4): 753–60. doi: 10.1016/S1097-2765(00)80254-3 . PMID   10882111.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Sato S, Tomomori-Sato C, Banks CA, et al. (2003). "Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3". J. Biol. Chem. 278 (17): 15123–7. doi: 10.1074/jbc.C300054200 . PMID   12584197.
• Sato S, Tomomori-Sato C, Banks CA, et al. (2004). "A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex". J. Biol. Chem. 278 (50): 49671–4. doi: 10.1074/jbc.C300444200 . PMID   14576168.
• Tomomori-Sato C, Sato S, Parmely TJ, et al. (2004). "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2". J. Biol. Chem. 279 (7): 5846–51. doi: 10.1074/jbc.M312523200 . PMID   14638676.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi: 10.1038/ng1285 . PMID   14702039.
• Humphray SJ, Oliver K, Hunt AR, et al. (2004). "DNA sequence and analysis of human chromosome 9". Nature. 429 (6990): 369–74. Bibcode:2004Natur.429..369H. doi:10.1038/nature02465. PMC   2734081 . PMID   15164053.
• Sato S, Tomomori-Sato C, Parmely TJ, et al. (2004). "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology". Mol. Cell. 14 (5): 685–91. doi: 10.1016/j.molcel.2004.05.006 . PMID   15175163.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC   528928 . PMID   15489334.